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P28321

- MGLL_YEAST

UniProt

P28321 - MGLL_YEAST

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Protein

Monoglyceride lipase

Gene

YJU3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Converts monoacylglycerides (MAG) to free fatty acids and glycerol. Required for efficient degradation of MAG, short-lived intermediates of glycerolipid metabolism which may also function as lipid signaling molecules. Controls inactivation of the signaling lipid N-palmitoylethanolamine (PEA).2 Publications

Catalytic activityi

Hydrolyzes glycerol monoesters of long-chain fatty acids.

Kineticsi

  1. KM=260 µM for rac-13-oleoylglycerol1 Publication

Vmax=10.3 mmol/h/mg enzyme1 Publication

pH dependencei

Active from pH 4.5 to 8.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei123 – 1231NucleophileBy similarity
Active sitei251 – 2511Charge relay systemBy similarity
Active sitei281 – 2811Charge relay systemBy similarity

GO - Molecular functioni

  1. acylglycerol lipase activity Source: SGD

GO - Biological processi

  1. triglyceride catabolic process Source: UniProtKB-UniPathway
  2. triglyceride metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciMetaCyc:G3O-31885-MONOMER.
YEAST:G3O-31885-MONOMER.
ReactomeiREACT_189024. Acyl chain remodeling of DAG and TAG.
UniPathwayiUPA00256.

Protein family/group databases

MEROPSiS33.993.

Names & Taxonomyi

Protein namesi
Recommended name:
Monoglyceride lipase (EC:3.1.1.23)
Short name:
MGL
Alternative name(s):
Monoacylglycerol hydrolase
Short name:
MAG hydrolase
Short name:
MGH
Monoacylglycerol lipase
Short name:
MAG lipase
Short name:
MAGL
Serine hydrolase YJU3
Gene namesi
Name:YJU3
Ordered Locus Names:YKL094W
ORF Names:YKL441
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XI

Organism-specific databases

SGDiS000001577. YJU3.

Subcellular locationi

Cytoplasm. Endoplasmic reticulum. Lipid droplet. Mitochondrion outer membrane
Note: Although the protein was identified in the cytoplasm, several membrane systems and lipid droplets, MGL activity was only measured in membrane fractions and lipid droplets.

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. lipid particle Source: SGD
  3. membrane Source: SGD
  4. mitochondrial outer membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Lipid droplet, Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 313313Monoglyceride lipasePRO_0000203164Add
BLAST

Proteomic databases

MaxQBiP28321.
PaxDbiP28321.
PeptideAtlasiP28321.

Expressioni

Gene expression databases

GenevestigatoriP28321.

Interactioni

Protein-protein interaction databases

BioGridi34039. 31 interactions.
IntActiP28321. 9 interactions.
MINTiMINT-4083889.
STRINGi4932.YKL094W.

Structurei

3D structure databases

ProteinModelPortaliP28321.
SMRiP28321. Positions 5-307.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2267.
GeneTreeiENSGT00390000011364.
HOGENOMiHOG000247969.
InParanoidiP28321.
OMAiNLSECKA.
OrthoDBiEOG7Q8CZ5.

Family and domain databases

Gene3Di3.40.50.1820. 2 hits.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
[Graphical view]
PfamiPF00561. Abhydrolase_1. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 2 hits.

Sequencei

Sequence statusi: Complete.

P28321-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAPYPYKVQT TVPELQYENF DGAKFGYMFW PVQNGTNEVR GRVLLIHGFG
60 70 80 90 100
EYTKIQFRLM DHLSLNGYES FTFDQRGAGV TSPGRSKGVT DEYHVFNDLE
110 120 130 140 150
HFVEKNLSEC KAKGIPLFMW GHSMGGGICL NYACQGKHKN EISGYIGSGP
160 170 180 190 200
LIILHPHTMY NKPTQIIAPL LAKFLPRVRI DTGLDLKGIT SDKAYRAFLG
210 220 230 240 250
SDPMSVPLYG SFRQIHDFMQ RGAKLYKNEN NYIQKNFAKD KPVIIMHGQD
260 270 280 290 300
DTINDPKGSE KFIQDCPSAD KELKLYPGAR HSIFSLETDK VFNTVFNDMK
310
QWLDKHTTTE AKP
Length:313
Mass (Da):35,563
Last modified:October 5, 2010 - v2
Checksum:i5E0C3B431406DDF0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti244 – 2441I → V in CAA46971. (PubMed:1626433)Curated
Sequence conflicti290 – 2901K → E in CAA46971. (PubMed:1626433)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X66245 Genomic DNA. Translation: CAA46971.1.
X71133 Genomic DNA. Translation: CAA50463.1.
Z28094 Genomic DNA. Translation: CAA81932.1.
BK006944 Genomic DNA. Translation: DAA09064.1.
PIRiS37921.
RefSeqiNP_012829.1. NM_001179660.1.

Genome annotation databases

EnsemblFungiiYKL094W; YKL094W; YKL094W.
GeneIDi853768.
KEGGisce:YKL094W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X66245 Genomic DNA. Translation: CAA46971.1 .
X71133 Genomic DNA. Translation: CAA50463.1 .
Z28094 Genomic DNA. Translation: CAA81932.1 .
BK006944 Genomic DNA. Translation: DAA09064.1 .
PIRi S37921.
RefSeqi NP_012829.1. NM_001179660.1.

3D structure databases

ProteinModelPortali P28321.
SMRi P28321. Positions 5-307.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34039. 31 interactions.
IntActi P28321. 9 interactions.
MINTi MINT-4083889.
STRINGi 4932.YKL094W.

Protein family/group databases

MEROPSi S33.993.

Proteomic databases

MaxQBi P28321.
PaxDbi P28321.
PeptideAtlasi P28321.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YKL094W ; YKL094W ; YKL094W .
GeneIDi 853768.
KEGGi sce:YKL094W.

Organism-specific databases

SGDi S000001577. YJU3.

Phylogenomic databases

eggNOGi COG2267.
GeneTreei ENSGT00390000011364.
HOGENOMi HOG000247969.
InParanoidi P28321.
OMAi NLSECKA.
OrthoDBi EOG7Q8CZ5.

Enzyme and pathway databases

UniPathwayi UPA00256 .
BioCyci MetaCyc:G3O-31885-MONOMER.
YEAST:G3O-31885-MONOMER.
Reactomei REACT_189024. Acyl chain remodeling of DAG and TAG.

Miscellaneous databases

NextBioi 974861.

Gene expression databases

Genevestigatori P28321.

Family and domain databases

Gene3Di 3.40.50.1820. 2 hits.
InterProi IPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
[Graphical view ]
Pfami PF00561. Abhydrolase_1. 1 hit.
[Graphical view ]
SUPFAMi SSF53474. SSF53474. 2 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of the novel essential gene YJU2 and two flanking reading frames located within a 3.2 kb EcoRI fragment from chromosome X of Saccharomyces cerevisiae."
    Forrova H., Kolarov J., Ghislain M., Goffeau A.
    Yeast 8:419-422(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "DNA sequence analysis of a 17 kb fragment of yeast chromosome XI physically localizes the MRB1 gene and reveals eight new open reading frames, including a homologue of the KIN1/KIN2 and SNF1 protein kinases."
    Pallier C., Valens M., Puzos V., Fukuhara H., Cheret G., Sor F., Bolotin-Fukuhara M.
    Yeast 9:1149-1155(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Identification and characterization of major lipid particle proteins of the yeast Saccharomyces cerevisiae."
    Athenstaedt K., Zweytick D., Jandrositz A., Kohlwein S.D., Daum G.
    J. Bacteriol. 181:6441-6448(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.
  6. Cited for: SUBCELLULAR LOCATION.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Synergistic computational and experimental proteomics approaches for more accurate detection of active serine hydrolases in yeast."
    Baxter S.M., Rosenblum J.S., Knutson S., Nelson M.R., Montimurro J.S., Di Gennaro J.A., Speir J.A., Burbaum J.J., Fetrow J.S.
    Mol. Cell. Proteomics 3:209-225(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION AS A SERINE HYDROLASE BY MASS SPECTROMETRY.
  10. "Proteomic analysis of the yeast mitochondrial outer membrane reveals accumulation of a subclass of preproteins."
    Zahedi R.P., Sickmann A., Boehm A.M., Winkler C., Zufall N., Schoenfisch B., Guiard B., Pfanner N., Meisinger C.
    Mol. Biol. Cell 17:1436-1450(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
  11. "Genetic manipulation of palmitoylethanolamide production and inactivation in Saccharomyces cerevisiae."
    Muccioli G.G., Sia A., Muchowski P.J., Stella N.
    PLoS ONE 4:E5942-E5942(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. "Identification of Yju3p as functional orthologue of mammalian monoglyceride lipase in the yeast Saccharomycescerevisiae."
    Heier C., Taschler U., Rengachari S., Oberer M., Wolinski H., Natter K., Kohlwein S.D., Leber R., Zimmermann R.
    Biochim. Biophys. Acta 1801:1063-1071(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiMGLL_YEAST
AccessioniPrimary (citable) accession number: P28321
Secondary accession number(s): D6VXJ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: October 5, 2010
Last modified: October 29, 2014
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2140 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3