P28311 (DEFA4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-defensin 4

Alternative name(s):
Defensin-related cryptdin-4

Gene names

Name:	Defa4
Synonyms:	Defcr4

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	92 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probably contributes to the antimicrobial barrier function of the small bowel mucosa.
Subcellular location	Secreted.
Tissue specificity	Paneth cells of the small bowel.
Sequence similarities	Belongs to the alpha-defensin family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Antibiotic Antimicrobial Defensin
PTM	Disulfide bond
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	defense response to bacterium Inferred from direct assay. Source: MGI
Cellular component	extracellular space Inferred from electronic annotation. Source: InterPro
Molecular function	phospholipid binding Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 19

19

Potential

Propeptide

20 – 58

39

PRO_0000006823

Peptide

59 – 92

34

Alpha-defensin 4

PRO_0000006824

Amino acid modifications

Disulfide bond

64 ↔ 89

Ref.6

Disulfide bond

66 ↔ 81

Ref.6

Disulfide bond

71 ↔ 88

Ref.6

Experimental info

Sequence conflict

30

1

K → N in AAI34361. Ref.2

Secondary structure

1

.

.

.

.

.

92

Helix Strand Turn

Details...

Beta strand

65 – 69

5

Beta strand

81 – 88

8

Sequences

	Sequence		Length	Mass (Da)
	P28311 [UniParc]. Last modified June 20, 2002. Version 4. Checksum: BF632838D52AFCE2	FASTA	92	10,272
	10 20 30 40 50 60 MKTLVLLSAL VLLAFQVQAD PIQNTDEETK TEEQPGEEDQ AVSISFGGQE GSALHEKSLR 70 80 90 GLLCYCRKGH CKRGERVRGT CGIRFLYCCP RR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Peptide localization and gene structure of cryptdin 4, a differentially expressed mouse Paneth cell alpha-defensin." Ouellette A.J., Darmoul D., Tran D., Huttner K.M., Yuan J., Selsted M.E. Infect. Immun. 67:6643-6651(1999) [PubMed: 10569786] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/SvJ.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"Mouse Paneth cell defensins: primary structures and antibacterial activities of numerous cryptdin isoforms." Ouellette A.J., Hsieh M.M., Nosek M.T., Cano-Gauci D.F., Huttner K.M., Buick R.N., Selsted M.E. Infect. Immun. 62:5040-5047(1994) [PubMed: 7927786] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 4-92. Strain: C3H/HeJ and Swiss Webster. Tissue: Intestinal crypt.
[4]	"Structure and diversity of the murine cryptdin gene family." Huttner K.M., Selsted M.E., Ouellette A.J. Genomics 19:448-453(1994) [PubMed: 8188287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 59-92. Strain: 129/SvJ and C3H/HeJ. Tissue: Small intestine.
[5]	"Enteric defensins: antibiotic peptide components of intestinal host defense." Selsted M.E., Miller S.I., Henschen A.H., Ouellette A.J. J. Cell Biol. 118:929-936(1992) [PubMed: 1500431] [Abstract] Cited for: PROTEIN SEQUENCE OF 61-92. Strain: CD-1. Tissue: Ileum and Jejunum.
[6]	"Solution structure of cryptdin-4, a mouse paneth cell alpha-defensin." Jing W., Hunter H.N., Tanabe H., Ouellette A.J., Vogel H.J. Biochemistry 43:15759-15766(2004) [PubMed: 15595831] [Abstract] Cited for: STRUCTURE BY NMR OF 61-92, DISULFIDE BONDS.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF178040 Genomic DNA. Translation: AAD51759.1.
BC134360 mRNA. Translation: AAI34361.1.
U03032 mRNA. Translation: AAA57172.1.

IPI

IPI00119476.

PIR

I48887.

RefSeq

NP_034169.1. NM_010039.1.

UniGene

Mm.465152.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1TV0	NMR	-	A	61-92	[»]
2GW9	NMR	-	A	61-92	[»]
2GWP	NMR	-	A	61-92	[»]

ProteinModelPortal

P28311.

SMR

P28311. Positions 61-92.

DisProt

DP00388.

ModBase

Search...

Protein family/group databases

TCDB

1.C.19.1.5. defensin family.

Proteomic databases

PRIDE

P28311.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

13238.

KEGG

mmu:13238.

Organism-specific databases

CTD

1669.

MGI

MGI:99584. Defa4.

Phylogenomic databases

eggNOG

roNOG17814.

HOVERGEN

HBG011703.

Gene expression databases

CleanEx

MM_DEFCR4.

Genevestigator

P28311.

Family and domain databases

InterPro

IPR016327. Alpha-defensin.
IPR006080. Defensin_beta/neutrophil.
IPR002366. Defensin_propep.
[Graphical view]

KO

K05230.

PANTHER

PTHR11876. PTHR11876. 1 hit.

Pfam

PF00879. Defensin_propep. 1 hit.
[Graphical view]

PIRSF

PIRSF001875. Alpha-defensin. 1 hit.

SMART

SM00048. DEFSN. 1 hit.
[Graphical view]

PROSITE

PS00269. DEFENSIN. False negative.
[Graphical view]

ProtoNet

Search...

Other

NextBio

283444.

PMAP-CutDB

P28311.

SOURCE

Search...

Entry information

Entry name

DEFA4_MOUSE

Accession

Primary (citable) accession number: P28311
Secondary accession number(s): A3KPC1, Q9QZL4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	June 20, 2002
Last modified:	November 16, 2011
This is version 93 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program