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Protein

Alpha-defensin 4

Gene

Defa4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probably contributes to the antimicrobial barrier function of the small bowel mucosa.

GO - Molecular functioni

  • phospholipid binding Source: MGI

GO - Biological processi

  • antibacterial humoral response Source: MGI
  • defense response to bacterium Source: MGI
  • membrane disruption in other organism Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Defensin

Protein family/group databases

TCDBi1.C.19.1.5. the defensin (defensin) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-defensin 4
Alternative name(s):
Defensin-related cryptdin-4
Gene namesi
Name:Defa4
Synonyms:Defcr4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:99584. Defa4.

Subcellular locationi

GO - Cellular componenti

  • extracellular space Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence analysisAdd
BLAST
Propeptidei20 – 5839PRO_0000006823Add
BLAST
Peptidei59 – 9234Alpha-defensin 4PRO_0000006824Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi64 ↔ 891 Publication
Disulfide bondi66 ↔ 811 Publication
Disulfide bondi71 ↔ 881 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP28311.

Miscellaneous databases

PMAP-CutDBP28311.

Expressioni

Tissue specificityi

Paneth cells of the small bowel.

Gene expression databases

CleanExiMM_DEFCR4.

Structurei

Secondary structure

1
92
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi65 – 695Combined sources
Beta strandi77 – 804Combined sources
Beta strandi81 – 888Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1TV0NMR-A61-92[»]
2GW9NMR-A61-92[»]
2GWPNMR-A61-92[»]
2LEWNMR-A61-92[»]
2LEYNMR-A61-92[»]
DisProtiDP00388.
ProteinModelPortaliP28311.
SMRiP28311. Positions 61-92.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP28311.

Family & Domainsi

Sequence similaritiesi

Belongs to the alpha-defensin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG011703.
InParanoidiP28311.
KOiK05230.
PhylomeDBiP28311.

Family and domain databases

InterProiIPR016327. Alpha-defensin_pro.
IPR006080. Defensin_beta/alpha.
IPR002366. Defensin_propep.
[Graphical view]
PANTHERiPTHR11876. PTHR11876. 1 hit.
PfamiPF00879. Defensin_propep. 1 hit.
[Graphical view]
PIRSFiPIRSF001875. Alpha-defensin. 1 hit.
SMARTiSM00048. DEFSN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28311-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTLVLLSAL VLLAFQVQAD PIQNTDEETK TEEQPGEEDQ AVSISFGGQE
60 70 80 90
GSALHEKSLR GLLCYCRKGH CKRGERVRGT CGIRFLYCCP RR
Length:92
Mass (Da):10,272
Last modified:June 20, 2002 - v4
Checksum:iBF632838D52AFCE2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti30 – 301K → N in AAI34361 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF178040 Genomic DNA. Translation: AAD51759.1.
BC134360 mRNA. Translation: AAI34361.1.
U03032 mRNA. Translation: AAA57172.1.
PIRiI48887.
RefSeqiNP_034169.2. NM_010039.2.
UniGeneiMm.426634.
Mm.465152.

Genome annotation databases

GeneIDi13238.
KEGGimmu:13238.
UCSCiuc029wro.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF178040 Genomic DNA. Translation: AAD51759.1.
BC134360 mRNA. Translation: AAI34361.1.
U03032 mRNA. Translation: AAA57172.1.
PIRiI48887.
RefSeqiNP_034169.2. NM_010039.2.
UniGeneiMm.426634.
Mm.465152.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1TV0NMR-A61-92[»]
2GW9NMR-A61-92[»]
2GWPNMR-A61-92[»]
2LEWNMR-A61-92[»]
2LEYNMR-A61-92[»]
DisProtiDP00388.
ProteinModelPortaliP28311.
SMRiP28311. Positions 61-92.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi1.C.19.1.5. the defensin (defensin) family.

Proteomic databases

PRIDEiP28311.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi13238.
KEGGimmu:13238.
UCSCiuc029wro.1. mouse.

Organism-specific databases

CTDi1669.
MGIiMGI:99584. Defa4.

Phylogenomic databases

HOVERGENiHBG011703.
InParanoidiP28311.
KOiK05230.
PhylomeDBiP28311.

Miscellaneous databases

EvolutionaryTraceiP28311.
NextBioi283444.
PMAP-CutDBP28311.
PROiP28311.
SOURCEiSearch...

Gene expression databases

CleanExiMM_DEFCR4.

Family and domain databases

InterProiIPR016327. Alpha-defensin_pro.
IPR006080. Defensin_beta/alpha.
IPR002366. Defensin_propep.
[Graphical view]
PANTHERiPTHR11876. PTHR11876. 1 hit.
PfamiPF00879. Defensin_propep. 1 hit.
[Graphical view]
PIRSFiPIRSF001875. Alpha-defensin. 1 hit.
SMARTiSM00048. DEFSN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Peptide localization and gene structure of cryptdin 4, a differentially expressed mouse Paneth cell alpha-defensin."
    Ouellette A.J., Darmoul D., Tran D., Huttner K.M., Yuan J., Selsted M.E.
    Infect. Immun. 67:6643-6651(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/SvJ.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. "Mouse Paneth cell defensins: primary structures and antibacterial activities of numerous cryptdin isoforms."
    Ouellette A.J., Hsieh M.M., Nosek M.T., Cano-Gauci D.F., Huttner K.M., Buick R.N., Selsted M.E.
    Infect. Immun. 62:5040-5047(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 4-92.
    Strain: C3H/HeJ and Swiss Webster.
    Tissue: Intestinal crypt.
  4. "Structure and diversity of the murine cryptdin gene family."
    Huttner K.M., Selsted M.E., Ouellette A.J.
    Genomics 19:448-453(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 59-92.
    Strain: 129/SvJ and C3H/HeJ.
    Tissue: Small intestine.
  5. "Enteric defensins: antibiotic peptide components of intestinal host defense."
    Selsted M.E., Miller S.I., Henschen A.H., Ouellette A.J.
    J. Cell Biol. 118:929-936(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 61-92.
    Strain: CD-1.
    Tissue: Ileum and Jejunum.
  6. "Solution structure of cryptdin-4, a mouse paneth cell alpha-defensin."
    Jing W., Hunter H.N., Tanabe H., Ouellette A.J., Vogel H.J.
    Biochemistry 43:15759-15766(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 61-92, DISULFIDE BONDS.

Entry informationi

Entry nameiDEFA4_MOUSE
AccessioniPrimary (citable) accession number: P28311
Secondary accession number(s): A3KPC1, Q9QZL4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: June 20, 2002
Last modified: May 11, 2016
This is version 115 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.