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Protein

Alpha-defensin 3

Gene

Defa3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probably contributes to the antimicrobial barrier function of the small bowel mucosa.

GO - Biological processi

  • cellular response to lipopolysaccharide Source: MGI
  • defense response to bacterium Source: MGI
  • defense response to Gram-negative bacterium Source: MGI
  • defense response to Gram-positive bacterium Source: MGI
  • response to drug Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Defensin

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-defensin 3
Alternative name(s):
Defensin-related cryptdin-3
Gene namesi
Name:Defa3
Synonyms:Defcr3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:94883. Defa3.

Subcellular locationi

GO - Cellular componenti

  • extracellular space Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1616Sequence analysisAdd
BLAST
Propeptidei17 – 58421 PublicationPRO_0000006821Add
BLAST
Peptidei59 – 9335Alpha-defensin 3PRO_0000006822Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi64 ↔ 92By similarity
Disulfide bondi66 ↔ 81By similarity
Disulfide bondi71 ↔ 91By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP28310.
PeptideAtlasiP28310.

PTM databases

iPTMnetiP28310.
PhosphoSiteiP28310.

Expressioni

Tissue specificityi

Paneth cells of the small bowel.

Gene expression databases

BgeeiP28310.
CleanExiMM_DEFCR3.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000096492.

Structurei

3D structure databases

ProteinModelPortaliP28310.
SMRiP28310. Positions 62-93.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alpha-defensin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410JFE2. Eukaryota.
ENOG4111A2R. LUCA.
GeneTreeiENSGT00840000130112.
HOVERGENiHBG011703.
InParanoidiP28310.
KOiK05230.
OMAiFACDKIS.
OrthoDBiEOG75TMFQ.
TreeFamiTF338414.

Family and domain databases

InterProiIPR006081. Alpha-defensin.
IPR016327. Alpha-defensin_pro.
IPR006080. Defensin_beta/alpha.
IPR002366. Defensin_propep.
[Graphical view]
PANTHERiPTHR11876. PTHR11876. 1 hit.
PfamiPF00323. Defensin_1. 1 hit.
PF00879. Defensin_propep. 1 hit.
[Graphical view]
PIRSFiPIRSF001875. Alpha-defensin. 1 hit.
SMARTiSM00048. DEFSN. 1 hit.
[Graphical view]
PROSITEiPS00269. DEFENSIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28310-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTLVLLSAL VLLAFQVQAD PIQNTDEETK TEEQPGEDDQ AVSVSFGDPE
60 70 80 90
GSSLQEESLR DLVCYCRKRG CKRRERMNGT CRKGHLMYTL CCR
Length:93
Mass (Da):10,528
Last modified:October 1, 1996 - v2
Checksum:i2B2D61CFCF40E634
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12559 Genomic DNA. Translation: AAA20972.1.
U02999, U02998 Genomic DNA. Translation: AAB60677.1.
U03030 mRNA. Translation: AAA57171.1.
BC099855 mRNA. Translation: AAH99855.1.
CCDSiCCDS40270.1.
PIRiI49102.
RefSeqiNP_001161262.1. NM_001167790.1.
NP_031876.1. NM_007850.2.
UniGeneiMm.425519.
Mm.436573.

Genome annotation databases

EnsembliENSMUST00000079528; ENSMUSP00000078485; ENSMUSG00000060208.
ENSMUST00000098893; ENSMUSP00000096492; ENSMUSG00000074440.
GeneIDi13237.
23855.
KEGGimmu:13237.
mmu:23855.
UCSCiuc009lbn.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12559 Genomic DNA. Translation: AAA20972.1.
U02999, U02998 Genomic DNA. Translation: AAB60677.1.
U03030 mRNA. Translation: AAA57171.1.
BC099855 mRNA. Translation: AAH99855.1.
CCDSiCCDS40270.1.
PIRiI49102.
RefSeqiNP_001161262.1. NM_001167790.1.
NP_031876.1. NM_007850.2.
UniGeneiMm.425519.
Mm.436573.

3D structure databases

ProteinModelPortaliP28310.
SMRiP28310. Positions 62-93.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000096492.

PTM databases

iPTMnetiP28310.
PhosphoSiteiP28310.

Proteomic databases

PaxDbiP28310.
PeptideAtlasiP28310.

Protocols and materials databases

DNASUi13237.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000079528; ENSMUSP00000078485; ENSMUSG00000060208.
ENSMUST00000098893; ENSMUSP00000096492; ENSMUSG00000074440.
GeneIDi13237.
23855.
KEGGimmu:13237.
mmu:23855.
UCSCiuc009lbn.2. mouse.

Organism-specific databases

CTDi1668.
23855.
MGIiMGI:94883. Defa3.

Phylogenomic databases

eggNOGiENOG410JFE2. Eukaryota.
ENOG4111A2R. LUCA.
GeneTreeiENSGT00840000130112.
HOVERGENiHBG011703.
InParanoidiP28310.
KOiK05230.
OMAiFACDKIS.
OrthoDBiEOG75TMFQ.
TreeFamiTF338414.

Miscellaneous databases

PROiP28310.
SOURCEiSearch...

Gene expression databases

BgeeiP28310.
CleanExiMM_DEFCR3.

Family and domain databases

InterProiIPR006081. Alpha-defensin.
IPR016327. Alpha-defensin_pro.
IPR006080. Defensin_beta/alpha.
IPR002366. Defensin_propep.
[Graphical view]
PANTHERiPTHR11876. PTHR11876. 1 hit.
PfamiPF00323. Defensin_1. 1 hit.
PF00879. Defensin_propep. 1 hit.
[Graphical view]
PIRSFiPIRSF001875. Alpha-defensin. 1 hit.
SMARTiSM00048. DEFSN. 1 hit.
[Graphical view]
PROSITEiPS00269. DEFENSIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure and diversity of the murine cryptdin gene family."
    Huttner K.M., Selsted M.E., Ouellette A.J.
    Genomics 19:448-453(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: 129.
    Tissue: Small intestine.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3-93.
  3. "Enteric defensins: antibiotic peptide components of intestinal host defense."
    Selsted M.E., Miller S.I., Henschen A.H., Ouellette A.J.
    J. Cell Biol. 118:929-936(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 59-93.
    Strain: SWR/J.
    Tissue: Small intestine.

Entry informationi

Entry nameiDEFA3_MOUSE
AccessioniPrimary (citable) accession number: P28310
Secondary accession number(s): Q499K7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: October 1, 1996
Last modified: July 6, 2016
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.