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Protein

Aminodeoxychorismate lyase

Gene

pabC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of p-aminobenzoate (PABA), a precursor of tetrahydrofolate. Converts 4-amino-4-deoxychorismate into 4-aminobenzoate (PABA) and pyruvate.2 Publications

Catalytic activityi

4-amino-4-deoxychorismate = 4-aminobenzoate + pyruvate.1 Publication

Cofactori

pyridoxal 5'-phosphate3 Publications

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes 4-aminobenzoate from chorismate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Aminodeoxychorismate synthase component 2 (pabA), Aminodeoxychorismate synthase component 1 (pabB)
  2. Aminodeoxychorismate lyase (pabC)
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-aminobenzoate from chorismate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

  • 4-amino-4-deoxychorismate lyase activity Source: UniProtKB
  • branched-chain-amino-acid transaminase activity Source: GO_Central
  • pyridoxal phosphate binding Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Folate biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciEcoCyc:ADCLY-MONOMER.
ECOL316407:JW1082-MONOMER.
MetaCyc:ADCLY-MONOMER.
BRENDAi4.1.3.38. 2026.
UniPathwayiUPA00077; UER00150.

Names & Taxonomyi

Protein namesi
Recommended name:
Aminodeoxychorismate lyase (EC:4.1.3.38)
Alternative name(s):
4-amino-4-deoxychorismate lyase
Short name:
ADC lyase
Short name:
ADCL
Gene namesi
Name:pabC
Ordered Locus Names:b1096, JW1082
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11493. pabC.

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene are unable to produce PABA.1 Publication

Chemistry databases

ChEMBLiCHEMBL1075099.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001033051 – 269Aminodeoxychorismate lyaseAdd BLAST269

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei140N6-(pyridoxal phosphate)lysine1

Proteomic databases

PaxDbiP28305.
PRIDEiP28305.

Interactioni

Subunit structurei

Homodimer.4 Publications

Protein-protein interaction databases

BioGridi4259420. 470 interactors.
DIPiDIP-10435N.
IntActiP28305. 8 interactors.
STRINGi511145.b1096.

Chemistry databases

BindingDBiP28305.

Structurei

Secondary structure

1269
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 4Combined sources3
Beta strandi7 – 10Combined sources4
Helixi17 – 21Combined sources5
Beta strandi24 – 32Combined sources9
Helixi39 – 52Combined sources14
Helixi60 – 74Combined sources15
Beta strandi76 – 84Combined sources9
Beta strandi101 – 107Combined sources7
Helixi112 – 119Combined sources8
Beta strandi121 – 125Combined sources5
Turni134 – 137Combined sources4
Helixi145 – 154Combined sources10
Turni155 – 158Combined sources4
Beta strandi160 – 166Combined sources7
Beta strandi169 – 173Combined sources5
Beta strandi175 – 183Combined sources9
Beta strandi186 – 190Combined sources5
Beta strandi193 – 197Combined sources5
Helixi200 – 211Combined sources12
Beta strandi212 – 219Combined sources8
Helixi223 – 227Combined sources5
Beta strandi230 – 235Combined sources6
Beta strandi237 – 239Combined sources3
Beta strandi241 – 247Combined sources7
Helixi256 – 266Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ET0X-ray2.20A1-269[»]
1I2KX-ray1.79A1-269[»]
1I2LX-ray2.30A1-269[»]
ProteinModelPortaliP28305.
SMRiP28305.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP28305.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105YAZ. Bacteria.
COG0115. LUCA.
HOGENOMiHOG000276707.
InParanoidiP28305.
KOiK02619.
OMAiGALVECC.
PhylomeDBiP28305.

Family and domain databases

CDDicd01559. ADCL_like. 1 hit.
InterProiIPR017824. Aminodeoxychorismate_lyase_IV.
IPR001544. Aminotrans_IV.
IPR018300. Aminotrans_IV_CS.
[Graphical view]
PANTHERiPTHR11825. PTHR11825. 1 hit.
PfamiPF01063. Aminotran_4. 1 hit.
[Graphical view]
SUPFAMiSSF56752. SSF56752. 1 hit.
TIGRFAMsiTIGR03461. pabC_Proteo. 1 hit.
PROSITEiPS00770. AA_TRANSFER_CLASS_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P28305-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFLINGHKQE SLAVSDRATQ FGDGCFTTAR VIDGKVSLLS AHIQRLQDAC
60 70 80 90 100
QRLMISCDFW PQLEQEMKTL AAEQQNGVLK VVISRGSGGR GYSTLNSGPA
110 120 130 140 150
TRILSVTAYP AHYDRLRNEG ITLALSPVRL GRNPHLAGIK HLNRLEQVLI
160 170 180 190 200
RSHLEQTNAD EALVLDSEGW VTECCAANLF WRKGNVVYTP RLDQAGVNGI
210 220 230 240 250
MRQFCIRLLA QSSYQLVEVQ ASLEESLQAD EMVICNALMP VMPVCACGDV
260
SFSSATLYEY LAPLCERPN
Length:269
Mass (Da):29,715
Last modified:December 1, 1992 - v1
Checksum:i4BC186083F5E999E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93135 Genomic DNA. Translation: AAA24267.1.
U00096 Genomic DNA. Translation: AAC74180.1.
AP009048 Genomic DNA. Translation: BAA35904.1.
PIRiA42954.
RefSeqiNP_415614.1. NC_000913.3.
WP_000478681.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74180; AAC74180; b1096.
BAA35904; BAA35904; BAA35904.
GeneIDi946647.
KEGGiecj:JW1082.
eco:b1096.
PATRICi32117433. VBIEscCol129921_1139.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93135 Genomic DNA. Translation: AAA24267.1.
U00096 Genomic DNA. Translation: AAC74180.1.
AP009048 Genomic DNA. Translation: BAA35904.1.
PIRiA42954.
RefSeqiNP_415614.1. NC_000913.3.
WP_000478681.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ET0X-ray2.20A1-269[»]
1I2KX-ray1.79A1-269[»]
1I2LX-ray2.30A1-269[»]
ProteinModelPortaliP28305.
SMRiP28305.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259420. 470 interactors.
DIPiDIP-10435N.
IntActiP28305. 8 interactors.
STRINGi511145.b1096.

Chemistry databases

BindingDBiP28305.
ChEMBLiCHEMBL1075099.

Proteomic databases

PaxDbiP28305.
PRIDEiP28305.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74180; AAC74180; b1096.
BAA35904; BAA35904; BAA35904.
GeneIDi946647.
KEGGiecj:JW1082.
eco:b1096.
PATRICi32117433. VBIEscCol129921_1139.

Organism-specific databases

EchoBASEiEB1456.
EcoGeneiEG11493. pabC.

Phylogenomic databases

eggNOGiENOG4105YAZ. Bacteria.
COG0115. LUCA.
HOGENOMiHOG000276707.
InParanoidiP28305.
KOiK02619.
OMAiGALVECC.
PhylomeDBiP28305.

Enzyme and pathway databases

UniPathwayiUPA00077; UER00150.
BioCyciEcoCyc:ADCLY-MONOMER.
ECOL316407:JW1082-MONOMER.
MetaCyc:ADCLY-MONOMER.
BRENDAi4.1.3.38. 2026.

Miscellaneous databases

EvolutionaryTraceiP28305.
PROiP28305.

Family and domain databases

CDDicd01559. ADCL_like. 1 hit.
InterProiIPR017824. Aminodeoxychorismate_lyase_IV.
IPR001544. Aminotrans_IV.
IPR018300. Aminotrans_IV_CS.
[Graphical view]
PANTHERiPTHR11825. PTHR11825. 1 hit.
PfamiPF01063. Aminotran_4. 1 hit.
[Graphical view]
SUPFAMiSSF56752. SSF56752. 1 hit.
TIGRFAMsiTIGR03461. pabC_Proteo. 1 hit.
PROSITEiPS00770. AA_TRANSFER_CLASS_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPABC_ECOLI
AccessioniPrimary (citable) accession number: P28305
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: November 2, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.