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Protein

Protein-lysine 6-oxidase

Gene

Lox

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin.
Regulator of Ras expression. May play a role in tumor suppression.

Catalytic activityi

[Protein]-L-lysine + O2 + H2O = [protein]-(S)-2-amino-6-oxohexanoate + NH3 + H2O2.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi286 – 2861CopperSequence analysis
Metal bindingi288 – 2881CopperSequence analysis
Metal bindingi290 – 2901CopperSequence analysis

GO - Molecular functioni

  • copper ion binding Source: Ensembl
  • protein-lysine 6-oxidase activity Source: MGI

GO - Biological processi

  • aorta development Source: MGI
  • blood vessel development Source: MGI
  • collagen fibril organization Source: MGI
  • elastic fiber assembly Source: MGI
  • heart development Source: MGI
  • lung development Source: MGI
  • response to drug Source: Ensembl
  • response to steroid hormone Source: Ensembl
  • wound healing Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Copper, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-1566948. Elastic fibre formation.
R-MMU-2243919. Crosslinking of collagen fibrils.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-lysine 6-oxidase (EC:1.4.3.13)
Alternative name(s):
Lysyl oxidase
Ras excision protein
Gene namesi
Name:Lox
Synonyms:Rrg
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:96817. Lox.

Subcellular locationi

GO - Cellular componenti

  • collagen trimer Source: MGI
  • extracellular space Source: UniProtKB-SubCell
  • nucleus Source: Ensembl
  • proteinaceous extracellular matrix Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence analysisAdd
BLAST
Propeptidei22 – 162141By similarityPRO_0000018522Add
BLAST
Chaini163 – 411249Protein-lysine 6-oxidasePRO_0000018523Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi91 – 911N-linked (GlcNAc...)Sequence analysis
Glycosylationi138 – 1381N-linked (GlcNAc...)Sequence analysis
Disulfide bondi232 ↔ 238By similarity
Disulfide bondi285 ↔ 334By similarity
Cross-linki314 ↔ 349Lysine tyrosylquinone (Lys-Tyr)By similarity
Disulfide bondi318 ↔ 324By similarity
Disulfide bondi345 ↔ 355By similarity
Modified residuei349 – 34912',4',5'-topaquinoneBy similarity
Disulfide bondi392 ↔ 406By similarity

Post-translational modificationi

The lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine.
Proteolytically activated by BMP1.

Keywords - PTMi

Disulfide bond, Glycoprotein, LTQ, TPQ

Proteomic databases

PaxDbiP28301.
PeptideAtlasiP28301.
PRIDEiP28301.

PTM databases

iPTMnetiP28301.
PhosphoSiteiP28301.

Expressioni

Gene expression databases

BgeeiENSMUSG00000024529.
CleanExiMM_LOX.
ExpressionAtlasiP28301. baseline and differential.
GenevisibleiP28301. MM.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
HSPA1BP081076EBI-642911,EBI-629985From a different organism.
Hspa1bP178792EBI-642911,EBI-397360
RAF1P040492EBI-642911,EBI-365996From a different organism.

Protein-protein interaction databases

BioGridi201191. 3 interactions.
IntActiP28301. 13 interactions.
MINTiMINT-1549500.
STRINGi10090.ENSMUSP00000025409.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni207 – 411205Lysyl-oxidase likeAdd
BLAST

Sequence similaritiesi

Belongs to the lysyl oxidase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IHTH. Eukaryota.
ENOG41101E4. LUCA.
HOGENOMiHOG000234262.
HOVERGENiHBG000226.
InParanoidiP28301.
KOiK00277.
OMAiYSDDNPY.
OrthoDBiEOG091G02XD.
PhylomeDBiP28301.
TreeFamiTF326061.

Family and domain databases

InterProiIPR001695. Lysyl_oxidase.
IPR019828. Lysyl_oxidase_CS.
[Graphical view]
PfamiPF01186. Lysyl_oxidase. 1 hit.
[Graphical view]
PRINTSiPR00074. LYSYLOXIDASE.
PROSITEiPS00926. LYSYL_OXIDASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28301-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRFAWAVLLL GPLQLCPLLR CAPQTPREPP AAPGAWRQTI QWENNGQVFS
60 70 80 90 100
LLSLGAQYQP QRRRDPSATA RRPDGDAASQ PRTPILLLRD NRTASTRART
110 120 130 140 150
PSPSGVAAGR PRPAARHWFQ AGFSPSGARD GASRRAANRT ASPQPPQLSN
160 170 180 190 200
LRPPSHIDRM VGDDPYNPYK YSDDNPYYNY YDTYERPRPG SRNRPGYGTG
210 220 230 240 250
YFQYGLPDLV PDPYYIQAST YVQKMSMYNL RCAAEENCLA SSAYRADVRD
260 270 280 290 300
YDHRVLLRFP QRVKNQGTSD FLPSRPRYSW EWHSCHQHYH SMDEFSHYDL
310 320 330 340 350
LDANTQRRVA EGHKASFCLE DTSCDYGYHR RFACTAHTQG LSPGCYDTYA
360 370 380 390 400
ADIDCQWIDI TDVQPGNYIL KVSVNPSYLV PESDYTNNVV RCDIRYTGHH
410
AYASGCTISP Y
Length:411
Mass (Da):46,701
Last modified:December 1, 1992 - v1
Checksum:iDBC0563A9C0AEB52
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti333 – 3331A → G in AAA99899 (PubMed:8100214).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M65142 mRNA. Translation: AAA19032.1.
M65143 mRNA. Translation: AAA20185.1.
L04262, L04263, L04264 Genomic DNA. Translation: AAA99899.1.
BC018439 mRNA. Translation: AAH18439.1.
CCDSiCCDS50289.1.
PIRiA47005. OXMSL.
RefSeqiNP_001273110.1. NM_001286181.1.
NP_001273111.1. NM_001286182.1.
NP_034858.2. NM_010728.3.
UniGeneiMm.172.
Mm.488403.

Genome annotation databases

EnsembliENSMUST00000025409; ENSMUSP00000025409; ENSMUSG00000024529.
ENSMUST00000171470; ENSMUSP00000129247; ENSMUSG00000024529.
GeneIDi16948.
KEGGimmu:16948.
UCSCiuc008exe.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M65142 mRNA. Translation: AAA19032.1.
M65143 mRNA. Translation: AAA20185.1.
L04262, L04263, L04264 Genomic DNA. Translation: AAA99899.1.
BC018439 mRNA. Translation: AAH18439.1.
CCDSiCCDS50289.1.
PIRiA47005. OXMSL.
RefSeqiNP_001273110.1. NM_001286181.1.
NP_001273111.1. NM_001286182.1.
NP_034858.2. NM_010728.3.
UniGeneiMm.172.
Mm.488403.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201191. 3 interactions.
IntActiP28301. 13 interactions.
MINTiMINT-1549500.
STRINGi10090.ENSMUSP00000025409.

PTM databases

iPTMnetiP28301.
PhosphoSiteiP28301.

Proteomic databases

PaxDbiP28301.
PeptideAtlasiP28301.
PRIDEiP28301.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025409; ENSMUSP00000025409; ENSMUSG00000024529.
ENSMUST00000171470; ENSMUSP00000129247; ENSMUSG00000024529.
GeneIDi16948.
KEGGimmu:16948.
UCSCiuc008exe.2. mouse.

Organism-specific databases

CTDi4015.
MGIiMGI:96817. Lox.

Phylogenomic databases

eggNOGiENOG410IHTH. Eukaryota.
ENOG41101E4. LUCA.
HOGENOMiHOG000234262.
HOVERGENiHBG000226.
InParanoidiP28301.
KOiK00277.
OMAiYSDDNPY.
OrthoDBiEOG091G02XD.
PhylomeDBiP28301.
TreeFamiTF326061.

Enzyme and pathway databases

ReactomeiR-MMU-1566948. Elastic fibre formation.
R-MMU-2243919. Crosslinking of collagen fibrils.

Miscellaneous databases

ChiTaRSiLox. mouse.
PROiP28301.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024529.
CleanExiMM_LOX.
ExpressionAtlasiP28301. baseline and differential.
GenevisibleiP28301. MM.

Family and domain databases

InterProiIPR001695. Lysyl_oxidase.
IPR019828. Lysyl_oxidase_CS.
[Graphical view]
PfamiPF01186. Lysyl_oxidase. 1 hit.
[Graphical view]
PRINTSiPR00074. LYSYLOXIDASE.
PROSITEiPS00926. LYSYL_OXIDASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLYOX_MOUSE
AccessioniPrimary (citable) accession number: P28301
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: September 7, 2016
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The propeptide plays a role in directing the deposition of this enzyme to elastic fibers, via interaction with tropoelastin.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.