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Protein

Protein-lysine 6-oxidase

Gene

Lox

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. Regulator of Ras expression. May play a role in tumor suppression. Plays a role in the aortic wall architecture (PubMed:27432961).1 Publication

Miscellaneous

The propeptide plays a role in directing the deposition of this enzyme to elastic fibers, via interaction with tropoelastin.

Catalytic activityi

[Protein]-L-lysine + O2 + H2O = [protein]-(S)-2-amino-6-oxohexanoate + NH3 + H2O2.1 Publication

Cofactori

Protein has several cofactor binding sites:
  • Cu cationBy similarity
  • lysine tyrosylquinone residueBy similarityNote: Contains 1 lysine tyrosylquinone.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi286CopperSequence analysis1
Metal bindingi288CopperSequence analysis1
Metal bindingi290CopperSequence analysis1

GO - Molecular functioni

  • copper ion binding Source: Ensembl
  • protein-lysine 6-oxidase activity Source: UniProtKB

GO - Biological processi

  • aorta development Source: MGI
  • blood vessel development Source: MGI
  • blood vessel morphogenesis Source: UniProtKB
  • collagen fibril organization Source: MGI
  • elastic fiber assembly Source: MGI
  • heart development Source: MGI
  • lung development Source: MGI
  • response to drug Source: Ensembl
  • response to steroid hormone Source: Ensembl
  • wound healing Source: Ensembl

Keywordsi

Molecular functionOxidoreductase
LigandCopper, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-1566948 Elastic fibre formation
R-MMU-2243919 Crosslinking of collagen fibrils

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-lysine 6-oxidase (EC:1.4.3.131 Publication)
Alternative name(s):
Lysyl oxidase
Ras excision protein
Gene namesi
Name:Lox
Synonyms:Rrg
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:96817 Lox

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi292M → R: Homozygous mice display tortuous vessels aneurysm, loss of oxidase activity and die perinatally of aortic aneurysm and dissection. Heterozygous mice display thicker arterial wall with discontinuous elastic lamellae. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
PropeptideiPRO_000001852222 – 162By similarityAdd BLAST141
ChainiPRO_0000018523163 – 411Protein-lysine 6-oxidaseAdd BLAST249

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi91N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi138N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi232 ↔ 238By similarity
Disulfide bondi285 ↔ 334By similarity
Cross-linki314 ↔ 349Lysine tyrosylquinone (Lys-Tyr)By similarity
Disulfide bondi318 ↔ 324By similarity
Disulfide bondi345 ↔ 355By similarity
Modified residuei3492',4',5'-topaquinoneBy similarity1
Disulfide bondi392 ↔ 406By similarity

Post-translational modificationi

The lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine.By similarity
Proteolytically activated by BMP1.

Keywords - PTMi

Disulfide bond, Glycoprotein, LTQ, TPQ

Proteomic databases

PaxDbiP28301
PeptideAtlasiP28301
PRIDEiP28301

PTM databases

iPTMnetiP28301
PhosphoSitePlusiP28301

Expressioni

Tissue specificityi

Expressed in aorta (at protein level). Expressed in embryonic, juvenil and adult aorta.1 Publication

Gene expression databases

BgeeiENSMUSG00000024529
CleanExiMM_LOX
ExpressionAtlasiP28301 baseline and differential
GenevisibleiP28301 MM

Interactioni

Subunit structurei

Interacts with MFAP4.By similarity

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi201191, 4 interactors
IntActiP28301, 13 interactors
MINTiP28301
STRINGi10090.ENSMUSP00000025409

Structurei

3D structure databases

ProteinModelPortaliP28301
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni207 – 411Lysyl-oxidase likeAdd BLAST205

Sequence similaritiesi

Belongs to the lysyl oxidase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IHTH Eukaryota
ENOG41101E4 LUCA
GeneTreeiENSGT00900000140830
HOGENOMiHOG000234262
HOVERGENiHBG000226
InParanoidiP28301
KOiK00277
OMAiYSDDNPY
OrthoDBiEOG091G02XD
PhylomeDBiP28301
TreeFamiTF326061

Family and domain databases

InterProiView protein in InterPro
IPR001695 Lysyl_oxidase
IPR019828 Lysyl_oxidase_CS
PfamiView protein in Pfam
PF01186 Lysyl_oxidase, 1 hit
PRINTSiPR00074 LYSYLOXIDASE
PROSITEiView protein in PROSITE
PS00926 LYSYL_OXIDASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28301-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRFAWAVLLL GPLQLCPLLR CAPQTPREPP AAPGAWRQTI QWENNGQVFS
60 70 80 90 100
LLSLGAQYQP QRRRDPSATA RRPDGDAASQ PRTPILLLRD NRTASTRART
110 120 130 140 150
PSPSGVAAGR PRPAARHWFQ AGFSPSGARD GASRRAANRT ASPQPPQLSN
160 170 180 190 200
LRPPSHIDRM VGDDPYNPYK YSDDNPYYNY YDTYERPRPG SRNRPGYGTG
210 220 230 240 250
YFQYGLPDLV PDPYYIQAST YVQKMSMYNL RCAAEENCLA SSAYRADVRD
260 270 280 290 300
YDHRVLLRFP QRVKNQGTSD FLPSRPRYSW EWHSCHQHYH SMDEFSHYDL
310 320 330 340 350
LDANTQRRVA EGHKASFCLE DTSCDYGYHR RFACTAHTQG LSPGCYDTYA
360 370 380 390 400
ADIDCQWIDI TDVQPGNYIL KVSVNPSYLV PESDYTNNVV RCDIRYTGHH
410
AYASGCTISP Y
Length:411
Mass (Da):46,701
Last modified:December 1, 1992 - v1
Checksum:iDBC0563A9C0AEB52
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti333A → G in AAA99899 (PubMed:8100214).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M65142 mRNA Translation: AAA19032.1
M65143 mRNA Translation: AAA20185.1
L04262, L04263, L04264 Genomic DNA Translation: AAA99899.1
BC018439 mRNA Translation: AAH18439.1
CCDSiCCDS50289.1
PIRiA47005 OXMSL
RefSeqiNP_001273110.1, NM_001286181.1
NP_001273111.1, NM_001286182.1
NP_034858.2, NM_010728.3
UniGeneiMm.172
Mm.488403

Genome annotation databases

EnsembliENSMUST00000025409; ENSMUSP00000025409; ENSMUSG00000024529
ENSMUST00000171470; ENSMUSP00000129247; ENSMUSG00000024529
GeneIDi16948
KEGGimmu:16948
UCSCiuc008exe.2 mouse

Similar proteinsi

Entry informationi

Entry nameiLYOX_MOUSE
AccessioniPrimary (citable) accession number: P28301
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: May 23, 2018
This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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