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P28299

- ACEA_NEUCR

UniProt

P28299 - ACEA_NEUCR

Protein

Isocitrate lyase

Gene

acu-3

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 2 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Isocitrate = succinate + glyoxylate.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei215 – 2151Curated

    GO - Molecular functioni

    1. isocitrate lyase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-UniPathway
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Enzyme and pathway databases

    UniPathwayiUPA00703; UER00719.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isocitrate lyase (EC:4.1.3.1)
    Short name:
    ICL
    Short name:
    Isocitrase
    Short name:
    Isocitratase
    Gene namesi
    Name:acu-3
    ORF Names:2E4.10, NCU04230
    OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
    Taxonomic identifieri367110 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
    ProteomesiUP000001805: Chromosome 2, Linkage Group V

    Subcellular locationi

    GO - Cellular componenti

    1. glyoxysome Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Glyoxysome, Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 548548Isocitrate lyasePRO_0000068794Add
    BLAST

    Expressioni

    Inductioni

    By acetate.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    STRINGi5141.NCU04230.1.

    Structurei

    3D structure databases

    ProteinModelPortaliP28299.
    SMRiP28299. Positions 16-531.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG2224.
    HOGENOMiHOG000238475.
    KOiK01637.
    OMAiALISHQF.
    OrthoDBiEOG73Z331.

    Family and domain databases

    Gene3Di3.20.20.60. 2 hits.
    InterProiIPR006254. Isocitrate_lyase.
    IPR018523. Isocitrate_lyase_ph_CS.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    [Graphical view]
    PANTHERiPTHR21631:SF3. PTHR21631:SF3. 1 hit.
    PfamiPF00463. ICL. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001362. Isocit_lyase. 1 hit.
    SUPFAMiSSF51621. SSF51621. 1 hit.
    TIGRFAMsiTIGR01346. isocit_lyase. 1 hit.
    PROSITEiPS00161. ISOCITRATE_LYASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P28299-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAANNMVNPA VDPALEDELF AKEVEEVKKW WSDSRWRQTK RPFTAEQIVS    50
    KRGNLKIEYA SNAQAKKLWK ILEDRFAKRD ASYTYGCLEP TMVTQMAKYL 100
    DTVYVSGWQS SSTASSSDEP GPDLADYPYT TVPNKVGHLF MAQLFHDRKQ 150
    RQERLSVPKD QREKLANIDY LRPIVADADT GHGGLTAVMK LTKLFIEKGA 200
    AGIHIEDQAP GTKKCGHMAG KVLVPIQEHI NRLVAIRAQA DIMGSDLLCI 250
    ARTDAEAATL ITTTIDPRDH AFILGCTNPD LEPLADLMMK AEAEGKTGAQ 300
    LQAIEDDWLA KADLKRFDEA VLDVIAKGKF SNAKDLAAKY QAAVKGKQIS 350
    NREARAIARQ LLGQEIFFDW ESPRTREGYY RLKGGCDCSI NRAISYAPYC 400
    DAIWMESKLP DYAQAEEFAK GVHAVWPEQK LAYNLSPSFN WKTAMGRDDQ 450
    ETYIRRLAKL GYCWQFITLA GLHTTALISD QFAKAYSKIG MRAYGELVQE 500
    PEIDNGVDVV KHQKWSGATY VDELQKMVTG GVSSTAAMGK GVTEDQFH 548
    Length:548
    Mass (Da):61,214
    Last modified:June 1, 2001 - v2
    Checksum:i4C3414CE0794D44C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti132 – 1321V → C in CAA44573. (PubMed:1531185)Curated
    Sequence conflicti286 – 2861D → H in CAA44573. (PubMed:1531185)Curated
    Sequence conflicti422 – 4243VHA → PR in CAA44573. (PubMed:1531185)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X62697 Genomic DNA. Translation: CAA44573.1.
    AL451022 Genomic DNA. Translation: CAC18302.1.
    CM002240 Genomic DNA. Translation: EAA31618.1.
    PIRiS26858.
    RefSeqiXP_960854.1. XM_955761.2.
    UniGeneiNcr.25128.

    Genome annotation databases

    EnsemblFungiiEFNCRT00000003928; EFNCRP00000003928; EFNCRG00000003923.
    GeneIDi3877001.
    KEGGincr:NCU04230.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X62697 Genomic DNA. Translation: CAA44573.1 .
    AL451022 Genomic DNA. Translation: CAC18302.1 .
    CM002240 Genomic DNA. Translation: EAA31618.1 .
    PIRi S26858.
    RefSeqi XP_960854.1. XM_955761.2.
    UniGenei Ncr.25128.

    3D structure databases

    ProteinModelPortali P28299.
    SMRi P28299. Positions 16-531.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5141.NCU04230.1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii EFNCRT00000003928 ; EFNCRP00000003928 ; EFNCRG00000003923 .
    GeneIDi 3877001.
    KEGGi ncr:NCU04230.

    Phylogenomic databases

    eggNOGi COG2224.
    HOGENOMi HOG000238475.
    KOi K01637.
    OMAi ALISHQF.
    OrthoDBi EOG73Z331.

    Enzyme and pathway databases

    UniPathwayi UPA00703 ; UER00719 .

    Family and domain databases

    Gene3Di 3.20.20.60. 2 hits.
    InterProi IPR006254. Isocitrate_lyase.
    IPR018523. Isocitrate_lyase_ph_CS.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    [Graphical view ]
    PANTHERi PTHR21631:SF3. PTHR21631:SF3. 1 hit.
    Pfami PF00463. ICL. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001362. Isocit_lyase. 1 hit.
    SUPFAMi SSF51621. SSF51621. 1 hit.
    TIGRFAMsi TIGR01346. isocit_lyase. 1 hit.
    PROSITEi PS00161. ISOCITRATE_LYASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of the glyoxysomal isocitrate lyase genes of Aspergillus nidulans (acuD) and Neurospora crassa (acu-3)."
      Gainey L.D.S., Connerton I.F., Lewis E.H., Turner G., Ballance D.J.
      Curr. Genet. 21:43-47(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 74A.
    2. "What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence."
      Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.
      Nucleic Acids Res. 31:1944-1954(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
    3. "The genome sequence of the filamentous fungus Neurospora crassa."
      Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
      , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
      Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

    Entry informationi

    Entry nameiACEA_NEUCR
    AccessioniPrimary (citable) accession number: P28299
    Secondary accession number(s): Q7RVC2, Q9HEI3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 121 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3