P28299 (ACEA_NEUCR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isocitrate lyase
Short name=ICL
Short name=Isocitrase
Short name=Isocitratase
EC=4.1.3.1

Gene names

Name:	acu-3
ORF Names:	2E4.10, NCU04230

Organism

Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) [Reference proteome]

Taxonomic identifier

367110 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › sordariomyceta › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora › Neurospora crassa

Protein attributes

Sequence length	548 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Isocitrate = succinate + glyoxylate.
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 1/2.
Subunit structure	Homotetramer By similarity.
Subcellular location	Glyoxysome.
Induction	By acetate.
Sequence similarities	Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Glyoxysome Peroxisome
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-UniPathway tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	glyoxysome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	isocitrate lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 548	548	Isocitrate lyase		PRO_0000068794
Sites
	Active site	215	1	Probable
Experimental info
	Sequence conflict	132	1	V → C in CAA44573. Ref.1
	Sequence conflict	286	1	D → H in CAA44573. Ref.1
	Sequence conflict	422 – 424	3	VHA → PR in CAA44573. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P28299 [UniParc]. Last modified June 1, 2001. Version 2. Checksum: 4C3414CE0794D44C	FASTA	548	61,214
	10 20 30 40 50 60 MAANNMVNPA VDPALEDELF AKEVEEVKKW WSDSRWRQTK RPFTAEQIVS KRGNLKIEYA 70 80 90 100 110 120 SNAQAKKLWK ILEDRFAKRD ASYTYGCLEP TMVTQMAKYL DTVYVSGWQS SSTASSSDEP 130 140 150 160 170 180 GPDLADYPYT TVPNKVGHLF MAQLFHDRKQ RQERLSVPKD QREKLANIDY LRPIVADADT 190 200 210 220 230 240 GHGGLTAVMK LTKLFIEKGA AGIHIEDQAP GTKKCGHMAG KVLVPIQEHI NRLVAIRAQA 250 260 270 280 290 300 DIMGSDLLCI ARTDAEAATL ITTTIDPRDH AFILGCTNPD LEPLADLMMK AEAEGKTGAQ 310 320 330 340 350 360 LQAIEDDWLA KADLKRFDEA VLDVIAKGKF SNAKDLAAKY QAAVKGKQIS NREARAIARQ 370 380 390 400 410 420 LLGQEIFFDW ESPRTREGYY RLKGGCDCSI NRAISYAPYC DAIWMESKLP DYAQAEEFAK 430 440 450 460 470 480 GVHAVWPEQK LAYNLSPSFN WKTAMGRDDQ ETYIRRLAKL GYCWQFITLA GLHTTALISD 490 500 510 520 530 540 QFAKAYSKIG MRAYGELVQE PEIDNGVDVV KHQKWSGATY VDELQKMVTG GVSSTAAMGK GVTEDQFH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the glyoxysomal isocitrate lyase genes of Aspergillus nidulans (acuD) and Neurospora crassa (acu-3)." Gainey L.D.S., Connerton I.F., Lewis E.H., Turner G., Ballance D.J. Curr. Genet. 21:43-47(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 74A.
[2]	"What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence." Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U. Nucleic Acids Res. 31:1944-1954(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[3]	"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X62697 Genomic DNA. Translation: CAA44573.1. AL451022 Genomic DNA. Translation: CAC18302.1. AABX02000013 Genomic DNA. Translation: EAA31618.1.
PIR	S26858.
RefSeq	XP_960854.1. XM_955761.2.
UniGene	Ncr.25128.
3D structure databases
ProteinModelPortal	P28299.
SMR	P28299. Positions 16-531.
ModBase	Search...
Protein-protein interaction databases
STRING	5141.NCU04230.1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	EFNCRT00000003928; EFNCRP00000003928; EFNCRG00000003923.
GeneID	3877001.
KEGG	ncr:NCU04230.
Phylogenomic databases
eggNOG	COG2224.
HOGENOM	HOG000238475.
KO	K01637.
OMA	MVTQMAK.
OrthoDB	EOG4P5PJP.
Enzyme and pathway databases
UniPathway	UPA00703; UER00719.
Family and domain databases
Gene3D	3.20.20.60. 2 hits.
InterPro	IPR006254. Isocitrate_lyase. IPR000918. Isocitrate_lyase/Pmutase. IPR018523. Isocitrate_lyase_ph_CS. IPR015813. Pyrv/PenolPyrv_Kinase. [Graphical view]
PANTHER	PTHR21631:SF3. PTHR21631:SF3. 1 hit.
Pfam	PF00463. ICL. 1 hit. [Graphical view]
PIRSF	PIRSF001362. Isocit_lyase. 1 hit.
SUPFAM	SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMs	TIGR01346. isocit_lyase. 1 hit.
PROSITE	PS00161. ISOCITRATE_LYASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACEA_NEUCR

Accession

Primary (citable) accession number: P28299
Secondary accession number(s): Q7RVC2, Q9HEI3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	June 1, 2001
Last modified:	April 3, 2013
This is version 113 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program