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P28299 (ACEA_NEUCR) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Isocitrate lyase

Short name=ICL
Short name=Isocitrase
Short name=Isocitratase
EC=4.1.3.1
Gene names
Name:acu-3
ORF Names:2E4.10, NCU04230
OrganismNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) [Reference proteome]
Taxonomic identifier367110 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora

Protein attributes

Sequence length548 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Isocitrate = succinate + glyoxylate.

Pathway

Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 1/2.

Subunit structure

Homotetramer By similarity.

Subcellular location

Glyoxysome.

Induction

By acetate.

Sequence similarities

Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family.

Ontologies

Keywords
   Biological processGlyoxylate bypass
Tricarboxylic acid cycle
   Cellular componentGlyoxysome
Peroxisome
   Molecular functionLyase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglyoxylate cycle

Inferred from electronic annotation. Source: UniProtKB-UniPathway

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentglyoxysome

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionisocitrate lyase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 548548Isocitrate lyase
PRO_0000068794

Sites

Active site2151 Probable

Experimental info

Sequence conflict1321V → C in CAA44573. Ref.1
Sequence conflict2861D → H in CAA44573. Ref.1
Sequence conflict422 – 4243VHA → PR in CAA44573. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P28299 [UniParc].

Last modified June 1, 2001. Version 2.
Checksum: 4C3414CE0794D44C

FASTA54861,214
        10         20         30         40         50         60 
MAANNMVNPA VDPALEDELF AKEVEEVKKW WSDSRWRQTK RPFTAEQIVS KRGNLKIEYA 

        70         80         90        100        110        120 
SNAQAKKLWK ILEDRFAKRD ASYTYGCLEP TMVTQMAKYL DTVYVSGWQS SSTASSSDEP 

       130        140        150        160        170        180 
GPDLADYPYT TVPNKVGHLF MAQLFHDRKQ RQERLSVPKD QREKLANIDY LRPIVADADT 

       190        200        210        220        230        240 
GHGGLTAVMK LTKLFIEKGA AGIHIEDQAP GTKKCGHMAG KVLVPIQEHI NRLVAIRAQA 

       250        260        270        280        290        300 
DIMGSDLLCI ARTDAEAATL ITTTIDPRDH AFILGCTNPD LEPLADLMMK AEAEGKTGAQ 

       310        320        330        340        350        360 
LQAIEDDWLA KADLKRFDEA VLDVIAKGKF SNAKDLAAKY QAAVKGKQIS NREARAIARQ 

       370        380        390        400        410        420 
LLGQEIFFDW ESPRTREGYY RLKGGCDCSI NRAISYAPYC DAIWMESKLP DYAQAEEFAK 

       430        440        450        460        470        480 
GVHAVWPEQK LAYNLSPSFN WKTAMGRDDQ ETYIRRLAKL GYCWQFITLA GLHTTALISD 

       490        500        510        520        530        540 
QFAKAYSKIG MRAYGELVQE PEIDNGVDVV KHQKWSGATY VDELQKMVTG GVSSTAAMGK 


GVTEDQFH 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of the glyoxysomal isocitrate lyase genes of Aspergillus nidulans (acuD) and Neurospora crassa (acu-3)."
Gainey L.D.S., Connerton I.F., Lewis E.H., Turner G., Ballance D.J.
Curr. Genet. 21:43-47(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 74A.
[2]"What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence."
Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.
Nucleic Acids Res. 31:1944-1954(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[3]"The genome sequence of the filamentous fungus Neurospora crassa."
Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. expand/collapse author list , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X62697 Genomic DNA. Translation: CAA44573.1.
AL451022 Genomic DNA. Translation: CAC18302.1.
CM002240 Genomic DNA. Translation: EAA31618.1.
PIRS26858.
RefSeqXP_960854.1. XM_955761.2.
UniGeneNcr.25128.

3D structure databases

ProteinModelPortalP28299.
SMRP28299. Positions 16-531.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5141.NCU04230.1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiEFNCRT00000003928; EFNCRP00000003928; EFNCRG00000003923.
GeneID3877001.
KEGGncr:NCU04230.

Phylogenomic databases

eggNOGCOG2224.
HOGENOMHOG000238475.
KOK01637.
OMAALISHQF.
OrthoDBEOG73Z331.

Enzyme and pathway databases

UniPathwayUPA00703; UER00719.

Family and domain databases

Gene3D3.20.20.60. 2 hits.
InterProIPR006254. Isocitrate_lyase.
IPR018523. Isocitrate_lyase_ph_CS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PANTHERPTHR21631:SF3. PTHR21631:SF3. 1 hit.
PfamPF00463. ICL. 1 hit.
[Graphical view]
PIRSFPIRSF001362. Isocit_lyase. 1 hit.
SUPFAMSSF51621. SSF51621. 1 hit.
TIGRFAMsTIGR01346. isocit_lyase. 1 hit.
PROSITEPS00161. ISOCITRATE_LYASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameACEA_NEUCR
AccessionPrimary (citable) accession number: P28299
Secondary accession number(s): Q7RVC2, Q9HEI3
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: June 1, 2001
Last modified: May 14, 2014
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways