P28297 (ACEA_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isocitrate lyase
Short name=ICL
Short name=Isocitrase
Short name=Isocitratase
EC=4.1.3.1

Gene names

Name:	ICL
Ordered Locus Names:	At3g21720
ORF Names:	MSD21.3

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	576 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in storage lipid mobilization during the growth of higher plant seedling.
Catalytic activity	Isocitrate = succinate + glyoxylate.
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 1/2.
Subunit structure	Homotetramer.
Subcellular location	Glyoxysome.
Sequence similarities	Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Glyoxysome Peroxisome
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-UniPathway tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	glyoxysome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	isocitrate lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BRL1	Q9ZPS9	1	EBI-2293945,EBI-2292728

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 576	576	Isocitrate lyase		PRO_0000068801
Sites
	Active site	213	1	Probable
Experimental info
	Sequence conflict	1 – 10	10	MAASFSVPSM → MIDKPNQ Ref.4
	Sequence conflict	452 – 455	4	QQMV → PLTC Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P28297 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: 528F15CE04DD4A87	FASTA	576	64,245
	10 20 30 40 50 60 MAASFSVPSM IMEEEGRFEA EVAEVQTWWS SERFKLTRRP YTARDVVALR GHLKQGYASN 70 80 90 100 110 120 EMAKKLWRTL KSHQANGTAS RTFGALDPVQ VTMMAKHLDT IYVSGWQCSS THTSTNEPGP 130 140 150 160 170 180 DLADYPYDTV PNKVEHLFFA QQYHDRKQRE ARMSMSREER TKTPFVDYLK PIIADGDTGF 190 200 210 220 230 240 GGTTATVKLC KLFVERGAAG VHIEDQSSVT KKCGHMAGKV LVAVSEHINR LVAARLQFDV 250 260 270 280 290 300 MGTETVLVAR TDAVAATLIQ SNIDARDHQF ILGATNPSLR GKSLSSLLAE GMTVGKNGPA 310 320 330 340 350 360 LQSIEDQWLG SAGLMTFSEA VVQAIKRMNL NENEKNQRLS EWLTHARYEN CLSNEQGRVL 370 380 390 400 410 420 AAKLGVTDLF WDWDLPRTRE GFYRFQGSVA AAVVRGWAFA QIADIIWMET ASPDLNECTQ 430 440 450 460 470 480 FAEGIKSKTP EVMLAYNLSP SFNWDASGMT DQQMVEFIPR IARLGYCWQF ITLAGFHADA 490 500 510 520 530 540 LVVDTFAKDY ARRGMLAYVE RIQREERTHG VDTLAHQKWS GANYYDRYLK TVQGGISSTA 550 560 570 AMGKGVTEEQ FKESWTRPGA DGMGEGTSLV VAKSRM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S. DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Isocitrate lyase gene from Arabidopsis thaliana." Bernhard W.R., Matile P. Submitted (FEB-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE OF 1-455.
[5]	"An inventory of 1152 expressed sequence tags obtained by partial sequencing of cDNAs from Arabidopsis thaliana." Hoefte H., Desprez T., Amselem J., Chiapello H., Rouze P., Caboche M., Moisan A., Jourjon M.-F., Charpenteau J.-L., Berthomieu P., Guerrier D., Giraudat J., Quigley F., Thomas F., Yu D.-Y., Mache R., Raynal M., Cooke R. , Grellet F., Delseny M., Parmentier Y., de Marcillac G., Gigot C., Fleck J., Philipps G., Axelos M., Bardet C., Tremousaygue D., Lescure B. Plant J. 4:1051-1061(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 50-152. Strain: cv. Columbia. Tissue: Seedling.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB025634 Genomic DNA. Translation: BAB02834.1. CP002686 Genomic DNA. Translation: AEE76544.1. AY140000 mRNA. Translation: AAM98142.1. BT010397 mRNA. Translation: AAQ56840.1. M83534 Genomic DNA. Translation: AAA32823.1. Z18772 mRNA. Translation: CAA79248.1.
IPI	IPI00516999.
RefSeq	NP_188809.2. NM_113067.3.
UniGene	At.38005.
3D structure databases
ProteinModelPortal	P28297.
SMR	P28297. Positions 9-535.
ModBase	Search...
Protein-protein interaction databases
IntAct	P28297. 2 interactions.
Proteomic databases
PaxDb	P28297.
PRIDE	P28297.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G21720.1; AT3G21720.1; AT3G21720.
GeneID	821726.
KEGG	ath:AT3G21720.
Organism-specific databases
TAIR	At3g21720.
Phylogenomic databases
eggNOG	COG2224.
HOGENOM	HOG000238475.
InParanoid	P28297.
KO	K01637.
OMA	MVTQMAK.
PhylomeDB	P28297.
ProtClustDB	PLN02892.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-1601.
UniPathway	UPA00703; UER00719.
Gene expression databases
ArrayExpress	P28297.
Genevestigator	P28297.
GermOnline	AT3G21720. Arabidopsis thaliana.
Family and domain databases
Gene3D	3.20.20.60. 2 hits.
InterPro	IPR006254. Isocitrate_lyase. IPR000918. Isocitrate_lyase/Pmutase. IPR018523. Isocitrate_lyase_ph_CS. IPR015813. Pyrv/PenolPyrv_Kinase-like_dom. [Graphical view]
PANTHER	PTHR21631:SF3. PTHR21631:SF3. 1 hit.
Pfam	PF00463. ICL. 1 hit. [Graphical view]
PIRSF	PIRSF001362. Isocit_lyase. 1 hit.
SUPFAM	SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMs	TIGR01346. isocit_lyase. 1 hit.
PROSITE	PS00161. ISOCITRATE_LYASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACEA_ARATH

Accession

Primary (citable) accession number: P28297
Secondary accession number(s): Q41948, Q9LSZ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 2000
Last modified:	May 29, 2013
This is version 111 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program