Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P28297

- ACEA_ARATH

UniProt

P28297 - ACEA_ARATH

Protein

Isocitrate lyase

Gene

ICL

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 2 (01 Dec 2000)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Involved in storage lipid mobilization during the growth of higher plant seedling.

    Catalytic activityi

    Isocitrate = succinate + glyoxylate.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei213 – 2131Curated

    GO - Molecular functioni

    1. isocitrate lyase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-UniPathway
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Enzyme and pathway databases

    BioCyciARA:AT3G21720-MONOMER.
    MetaCyc:MONOMER-1601.
    UniPathwayiUPA00703; UER00719.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isocitrate lyase (EC:4.1.3.1)
    Short name:
    ICL
    Short name:
    Isocitrase
    Short name:
    Isocitratase
    Gene namesi
    Name:ICL
    Ordered Locus Names:At3g21720
    ORF Names:MSD21.3
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G21720.

    Subcellular locationi

    GO - Cellular componenti

    1. glyoxysome Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Glyoxysome, Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 576576Isocitrate lyasePRO_0000068801Add
    BLAST

    Proteomic databases

    PaxDbiP28297.
    PRIDEiP28297.

    Expressioni

    Gene expression databases

    GenevestigatoriP28297.

    Interactioni

    Subunit structurei

    Homotetramer.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    BRL1Q9ZPS91EBI-2293945,EBI-2292728

    Protein-protein interaction databases

    BioGridi7058. 3 interactions.
    IntActiP28297. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliP28297.
    SMRiP28297. Positions 9-535.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG2224.
    HOGENOMiHOG000238475.
    InParanoidiP28297.
    KOiK01637.
    OMAiALISHQF.
    PhylomeDBiP28297.

    Family and domain databases

    Gene3Di3.20.20.60. 2 hits.
    InterProiIPR006254. Isocitrate_lyase.
    IPR018523. Isocitrate_lyase_ph_CS.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    [Graphical view]
    PANTHERiPTHR21631:SF3. PTHR21631:SF3. 1 hit.
    PfamiPF00463. ICL. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001362. Isocit_lyase. 1 hit.
    SUPFAMiSSF51621. SSF51621. 1 hit.
    TIGRFAMsiTIGR01346. isocit_lyase. 1 hit.
    PROSITEiPS00161. ISOCITRATE_LYASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P28297-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAASFSVPSM IMEEEGRFEA EVAEVQTWWS SERFKLTRRP YTARDVVALR    50
    GHLKQGYASN EMAKKLWRTL KSHQANGTAS RTFGALDPVQ VTMMAKHLDT 100
    IYVSGWQCSS THTSTNEPGP DLADYPYDTV PNKVEHLFFA QQYHDRKQRE 150
    ARMSMSREER TKTPFVDYLK PIIADGDTGF GGTTATVKLC KLFVERGAAG 200
    VHIEDQSSVT KKCGHMAGKV LVAVSEHINR LVAARLQFDV MGTETVLVAR 250
    TDAVAATLIQ SNIDARDHQF ILGATNPSLR GKSLSSLLAE GMTVGKNGPA 300
    LQSIEDQWLG SAGLMTFSEA VVQAIKRMNL NENEKNQRLS EWLTHARYEN 350
    CLSNEQGRVL AAKLGVTDLF WDWDLPRTRE GFYRFQGSVA AAVVRGWAFA 400
    QIADIIWMET ASPDLNECTQ FAEGIKSKTP EVMLAYNLSP SFNWDASGMT 450
    DQQMVEFIPR IARLGYCWQF ITLAGFHADA LVVDTFAKDY ARRGMLAYVE 500
    RIQREERTHG VDTLAHQKWS GANYYDRYLK TVQGGISSTA AMGKGVTEEQ 550
    FKESWTRPGA DGMGEGTSLV VAKSRM 576
    Length:576
    Mass (Da):64,245
    Last modified:December 1, 2000 - v2
    Checksum:i528F15CE04DD4A87
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1 – 1010MAASFSVPSM → MIDKPNQ1 PublicationCurated
    Sequence conflicti452 – 4554QQMV → PLTC1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB025634 Genomic DNA. Translation: BAB02834.1.
    CP002686 Genomic DNA. Translation: AEE76544.1.
    AY140000 mRNA. Translation: AAM98142.1.
    BT010397 mRNA. Translation: AAQ56840.1.
    M83534 Genomic DNA. Translation: AAA32823.1.
    Z18772 mRNA. Translation: CAA79248.1.
    RefSeqiNP_188809.2. NM_113067.3.
    UniGeneiAt.38005.

    Genome annotation databases

    EnsemblPlantsiAT3G21720.1; AT3G21720.1; AT3G21720.
    GeneIDi821726.
    KEGGiath:AT3G21720.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB025634 Genomic DNA. Translation: BAB02834.1 .
    CP002686 Genomic DNA. Translation: AEE76544.1 .
    AY140000 mRNA. Translation: AAM98142.1 .
    BT010397 mRNA. Translation: AAQ56840.1 .
    M83534 Genomic DNA. Translation: AAA32823.1 .
    Z18772 mRNA. Translation: CAA79248.1 .
    RefSeqi NP_188809.2. NM_113067.3.
    UniGenei At.38005.

    3D structure databases

    ProteinModelPortali P28297.
    SMRi P28297. Positions 9-535.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 7058. 3 interactions.
    IntActi P28297. 2 interactions.

    Proteomic databases

    PaxDbi P28297.
    PRIDEi P28297.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G21720.1 ; AT3G21720.1 ; AT3G21720 .
    GeneIDi 821726.
    KEGGi ath:AT3G21720.

    Organism-specific databases

    TAIRi AT3G21720.

    Phylogenomic databases

    eggNOGi COG2224.
    HOGENOMi HOG000238475.
    InParanoidi P28297.
    KOi K01637.
    OMAi ALISHQF.
    PhylomeDBi P28297.

    Enzyme and pathway databases

    UniPathwayi UPA00703 ; UER00719 .
    BioCyci ARA:AT3G21720-MONOMER.
    MetaCyc:MONOMER-1601.

    Miscellaneous databases

    PROi P28297.

    Gene expression databases

    Genevestigatori P28297.

    Family and domain databases

    Gene3Di 3.20.20.60. 2 hits.
    InterProi IPR006254. Isocitrate_lyase.
    IPR018523. Isocitrate_lyase_ph_CS.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    [Graphical view ]
    PANTHERi PTHR21631:SF3. PTHR21631:SF3. 1 hit.
    Pfami PF00463. ICL. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001362. Isocit_lyase. 1 hit.
    SUPFAMi SSF51621. SSF51621. 1 hit.
    TIGRFAMsi TIGR01346. isocit_lyase. 1 hit.
    PROSITEi PS00161. ISOCITRATE_LYASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
      Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
      DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Isocitrate lyase gene from Arabidopsis thaliana."
      Bernhard W.R., Matile P.
      Submitted (FEB-1992) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE OF 1-455.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 50-152.
      Strain: cv. Columbia.
      Tissue: Seedling.
    6. "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana."
      Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E., Rathjen J.P., Peck S.C.
      J. Proteomics 72:439-451(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiACEA_ARATH
    AccessioniPrimary (citable) accession number: P28297
    Secondary accession number(s): Q41948, Q9LSZ2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 121 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3