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Protein

Alkaline protease 1

Gene

alp1

Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Secreted alkaline protease that allows assimilation of proteinaceous substrates. Acts as a significant virulence factor in invasive aspergillosis. Involved in immune evasion from the human and mice complement systems during infection. Efficiently cleaves important components of the complement cascade such as such as C3, C4, C5, and C1q, as well as IgG, which leads to down-regulation of complement activation at the hyphal surface.3 Publications

Catalytic activityi

Hydrolysis of proteins with broad specificity, and of Bz-Arg-OEt > Ac-Tyr-OEt. Does not hydrolyze peptide amides.

pH dependencei

Optimum pH is 7-8.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei162Charge relay systemBy similarity1
Active sitei193Charge relay systemBy similarity1
Active sitei349Charge relay systemBy similarity1

GO - Molecular functioni

  • fibrinogen binding Source: ASPGD
  • IgE binding Source: UniProtKB
  • peptidase activity Source: UniProtKB
  • serine-type endopeptidase activity Source: InterPro
  • serine-type peptidase activity Source: UniProtKB

GO - Biological processi

  • active evasion of host immune response via regulation of host complement system Source: UniProtKB
  • complement activation Source: ASPGD
  • elastin catabolic process Source: ASPGD
  • pathogenesis Source: UniProtKB
  • protein catabolic process Source: ASPGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Virulence

Protein family/group databases

MEROPSiS08.053.

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline protease 1 (EC:3.4.21.63)
Short name:
ALP
Alternative name(s):
Aspergillopeptidase B
Aspergillus proteinase B
Elastase
Elastinolytic serine proteinase
Oryzin
Allergen: Asp f 13
Gene namesi
Name:alp1
Synonyms:alk1, alp
ORF Names:AFUA_4G11800
OrganismiNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Taxonomic identifieri330879 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000002530 Componenti: Chromosome 4

Organism-specific databases

EuPathDBiFungiDB:Afu4g11800.

Subcellular locationi

GO - Cellular componenti

  • extracellular region Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human. Binds to IgE via three immunodominant epitopes localized at the C-terminal part.

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei3111. Asp f 13.0101.
66. Asp f 13.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21By similarityAdd BLAST21
PropeptideiPRO_000002708222 – 1011 PublicationAdd BLAST80
ChainiPRO_0000027083102 – 403Alkaline protease 1Add BLAST302

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi253N-linked (GlcNAc...)Sequence analysis1
Glycosylationi307N-linked (GlcNAc...)Sequence analysis1
Glycosylationi367N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein, Zymogen

Proteomic databases

PRIDEiP28296.

Expressioni

Inductioni

Expression is controlled by the prtT transcription factor.2 Publications

Interactioni

GO - Molecular functioni

  • fibrinogen binding Source: ASPGD
  • IgE binding Source: UniProtKB

Structurei

3D structure databases

ProteinModelPortaliP28296.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini159 – 384Peptidase S8Add BLAST226

Sequence similaritiesi

Belongs to the peptidase S8 family.Curated
Contains 1 peptidase S8 domain.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000199176.
InParanoidiP28296.
KOiK18549.
OMAiINWRTAS.
OrthoDBiEOG092C4A4B.

Family and domain databases

Gene3Di3.30.70.80. 1 hit.
3.40.50.200. 1 hit.
InterProiIPR000209. Peptidase_S8/S53_dom.
IPR023827. Peptidase_S8_Asp-AS.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR009020. Propept_inh.
IPR010259. S8pro/Inhibitor_I9.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 1 hit.
PfamiPF05922. Inhibitor_I9. 1 hit.
PF00082. Peptidase_S8. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF52743. SSF52743. 1 hit.
SSF54897. SSF54897. 1 hit.
PROSITEiPS00136. SUBTILASE_ASP. 1 hit.
PS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28296-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSIKRTLLL LGAVLPAVFG APVQETRRAA QKIPGKYIVT FKPGTDTATI
60 70 80 90 100
ESHTLWATDL HKRNLERRDT TSGEPPVGIE KSYKIKDFAA YAGSFDDATI
110 120 130 140 150
EEIRKSADVA HVEEDQIWYL DALTTQKGAP WGLGSISHKG QASTDYIYDT
160 170 180 190 200
SAGAGTYAYV VDSGINVNHV EFESRASLAY NAAGGSHVDS IGHGTHVAGT
210 220 230 240 250
IGGKTYGVAK KTNLLSVKVF QGESSSTSII LDGFNWAVND IVSKGRTKKA
260 270 280 290 300
AINMSLGGGY SYAFNNAVEN AFDEGVLSVV AAGNENSDAS NTSPASAPNA
310 320 330 340 350
LTVAAINKSN ARASFSNYGS VVDIFAPGQD ILSAWIGSTT ATNTISGTSM
360 370 380 390 400
ATPHIVGLSV YLMGLENLSG PAAVTARIKE LATNGVVTNV KGSPNKLAYN

GNA
Length:403
Mass (Da):42,190
Last modified:December 6, 2005 - v2
Checksum:i2D900D1AE22CDD4A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti106 – 107SA → RG in CAA77666 (PubMed:1601287).Curated2
Sequence conflicti295A → R in AAB07672 (PubMed:8500876).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11580 Genomic DNA. Translation: CAA77666.1.
M99420 Genomic DNA. Translation: AAB07672.1.
AAHF01000005 Genomic DNA. Translation: EAL89613.1.
PIRiS22184.
RefSeqiXP_751651.1. XM_746558.1.

Genome annotation databases

EnsemblFungiiCADAFUAT00008008; CADAFUAP00008008; CADAFUAG00008008.
GeneIDi3509271.
KEGGiafm:AFUA_4G11800.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11580 Genomic DNA. Translation: CAA77666.1.
M99420 Genomic DNA. Translation: AAB07672.1.
AAHF01000005 Genomic DNA. Translation: EAL89613.1.
PIRiS22184.
RefSeqiXP_751651.1. XM_746558.1.

3D structure databases

ProteinModelPortaliP28296.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei3111. Asp f 13.0101.
66. Asp f 13.
MEROPSiS08.053.

Proteomic databases

PRIDEiP28296.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADAFUAT00008008; CADAFUAP00008008; CADAFUAG00008008.
GeneIDi3509271.
KEGGiafm:AFUA_4G11800.

Organism-specific databases

EuPathDBiFungiDB:Afu4g11800.

Phylogenomic databases

HOGENOMiHOG000199176.
InParanoidiP28296.
KOiK18549.
OMAiINWRTAS.
OrthoDBiEOG092C4A4B.

Family and domain databases

Gene3Di3.30.70.80. 1 hit.
3.40.50.200. 1 hit.
InterProiIPR000209. Peptidase_S8/S53_dom.
IPR023827. Peptidase_S8_Asp-AS.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR009020. Propept_inh.
IPR010259. S8pro/Inhibitor_I9.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 1 hit.
PfamiPF05922. Inhibitor_I9. 1 hit.
PF00082. Peptidase_S8. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF52743. SSF52743. 1 hit.
SSF54897. SSF54897. 1 hit.
PROSITEiPS00136. SUBTILASE_ASP. 1 hit.
PS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiORYZ_ASPFU
AccessioniPrimary (citable) accession number: P28296
Secondary accession number(s): Q4WQ71
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 6, 2005
Last modified: November 2, 2016
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.