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Protein

Alkaline protease 1

Gene

alp1

Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Secreted alkaline protease that allows assimilation of proteinaceous substrates. Acts as a significant virulence factor in invasive aspergillosis. Involved in immune evasion from the human and mice complement systems during infection. Efficiently cleaves important components of the complement cascade such as such as C3, C4, C5, and C1q, as well as IgG, which leads to down-regulation of complement activation at the hyphal surface.3 Publications

Catalytic activityi

Hydrolysis of proteins with broad specificity, and of Bz-Arg-OEt > Ac-Tyr-OEt. Does not hydrolyze peptide amides.

pH dependencei

Optimum pH is 7-8.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei162Charge relay systemBy similarity1
Active sitei193Charge relay systemBy similarity1
Active sitei349Charge relay systemBy similarity1

GO - Molecular functioni

  • fibrinogen binding Source: AspGD
  • IgE binding Source: UniProtKB
  • peptidase activity Source: UniProtKB
  • serine-type endopeptidase activity Source: InterPro
  • serine-type peptidase activity Source: UniProtKB

GO - Biological processi

  • active evasion of host immune response via regulation of host complement system Source: UniProtKB
  • complement activation Source: AspGD
  • elastin catabolic process Source: AspGD
  • pathogenesis Source: UniProtKB
  • protein catabolic process Source: AspGD

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
Biological processVirulence

Protein family/group databases

MEROPSiS08.053

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline protease 1 (EC:3.4.21.63)
Short name:
ALP
Alternative name(s):
Aspergillopeptidase B
Aspergillus proteinase B
Elastase
Elastinolytic serine proteinase
Oryzin
Allergen: Asp f 13
Gene namesi
Name:alp1
Synonyms:alk1, alp
ORF Names:AFUA_4G11800
OrganismiNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Taxonomic identifieri330879 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000002530 Componentsi: Chromosome 4, Unassembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:Afu4g11800

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human. Binds to IgE via three immunodominant epitopes localized at the C-terminal part.

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei3111 Asp f 13.0101
66 Asp f 13

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21By similarityAdd BLAST21
PropeptideiPRO_000002708222 – 1011 PublicationAdd BLAST80
ChainiPRO_0000027083102 – 403Alkaline protease 1Add BLAST302

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi253N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi307N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi367N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein, Zymogen

Expressioni

Inductioni

Expression is controlled by the prtT transcription factor.2 Publications

Interactioni

Protein-protein interaction databases

STRINGi5085.CADAFUBP00006700

Structurei

3D structure databases

ProteinModelPortaliP28296
SMRiP28296
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini159 – 384Peptidase S8Add BLAST226

Sequence similaritiesi

Belongs to the peptidase S8 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000199176
InParanoidiP28296
KOiK18549
OMAiHVDTLGH
OrthoDBiEOG092C4A4B

Family and domain databases

CDDicd04077 Peptidases_S8_PCSK9_Proteinase, 1 hit
Gene3Di3.30.70.80, 1 hit
3.40.50.200, 1 hit
InterProiView protein in InterPro
IPR034193 PCSK9_ProteinaseK-like
IPR000209 Peptidase_S8/S53_dom
IPR036852 Peptidase_S8/S53_dom_sf
IPR023827 Peptidase_S8_Asp-AS
IPR022398 Peptidase_S8_His-AS
IPR023828 Peptidase_S8_Ser-AS
IPR015500 Peptidase_S8_subtilisin-rel
IPR010259 S8pro/Inhibitor_I9
IPR037045 S8pro/Inhibitor_I9_sf
PfamiView protein in Pfam
PF05922 Inhibitor_I9, 1 hit
PF00082 Peptidase_S8, 1 hit
PRINTSiPR00723 SUBTILISIN
SUPFAMiSSF52743 SSF52743, 1 hit
PROSITEiView protein in PROSITE
PS00136 SUBTILASE_ASP, 1 hit
PS00137 SUBTILASE_HIS, 1 hit
PS00138 SUBTILASE_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28296-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSIKRTLLL LGAVLPAVFG APVQETRRAA QKIPGKYIVT FKPGTDTATI
60 70 80 90 100
ESHTLWATDL HKRNLERRDT TSGEPPVGIE KSYKIKDFAA YAGSFDDATI
110 120 130 140 150
EEIRKSADVA HVEEDQIWYL DALTTQKGAP WGLGSISHKG QASTDYIYDT
160 170 180 190 200
SAGAGTYAYV VDSGINVNHV EFESRASLAY NAAGGSHVDS IGHGTHVAGT
210 220 230 240 250
IGGKTYGVAK KTNLLSVKVF QGESSSTSII LDGFNWAVND IVSKGRTKKA
260 270 280 290 300
AINMSLGGGY SYAFNNAVEN AFDEGVLSVV AAGNENSDAS NTSPASAPNA
310 320 330 340 350
LTVAAINKSN ARASFSNYGS VVDIFAPGQD ILSAWIGSTT ATNTISGTSM
360 370 380 390 400
ATPHIVGLSV YLMGLENLSG PAAVTARIKE LATNGVVTNV KGSPNKLAYN

GNA
Length:403
Mass (Da):42,190
Last modified:December 6, 2005 - v2
Checksum:i2D900D1AE22CDD4A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti106 – 107SA → RG in CAA77666 (PubMed:1601287).Curated2
Sequence conflicti295A → R in AAB07672 (PubMed:8500876).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11580 Genomic DNA Translation: CAA77666.1
M99420 Genomic DNA Translation: AAB07672.1
AAHF01000005 Genomic DNA Translation: EAL89613.1
PIRiS22184
RefSeqiXP_751651.1, XM_746558.1

Genome annotation databases

EnsemblFungiiCADAFUAT00008008; CADAFUAP00008008; CADAFUAG00008008
GeneIDi3509271
KEGGiafm:AFUA_4G11800

Entry informationi

Entry nameiORYZ_ASPFU
AccessioniPrimary (citable) accession number: P28296
Secondary accession number(s): Q4WQ71
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 6, 2005
Last modified: May 23, 2018
This is version 131 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

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