Reviewed, UniProtKB/Swiss-Prot P28294 (PYRD_AGRAE)

Names and origin

Protein names

Recommended name:
    Dihydroorotate dehydrogenase, mitochondrial
      Short name=DHOdehase
      Short name=DHODase
      Short name=DHOD
    EC=1.3.5.2
Alternative name(s):
    Dihydroorotate oxidase

Gene names

Name:

URA1

Organism

Agrocybe aegerita (Black poplar mushroom)

Taxonomic identifier

5400 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Homobasidiomycetes › Agaricomycetidae › Agaricales › Bolbitiaceae › Agrocybe

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + a quinone = orotate + a quinol.
Cofactor	Binds 1 FMN per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1.
Subcellular location	Mitochondrion inner membrane By similarity; Single-pass membrane protein Potential.
Sequence similarities	Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide Transmembrane
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro UMP biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	dihydroorotate oxidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 328		Dihydroorotate dehydrogenase, mitochondrial		PRO_0000148499
Regions
	Transmembrane	21 – 38	18	Potential
Sites
	Active site	173	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	P28294-1 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 71FA3D2A4D57EF75	FASTA	328	35,085
	10 20 30 40 50 60 MSGLFETLGR RALFAFDAEQ AHGLSIAGLK TGLVTGSAPN DLALSVKVAG LQFPNPLGMA 70 80 90 100 110 120 AGYDKNAEVP DALLKLGFGF TEIGTITPRP QSGNPRPRIF RLVDDKAVIN RLGFNNEGHD 130 140 150 160 170 180 AAFKRLSQRA GKSGIVGVNI GANKDAEDRI ADYVAGIRRF YQLARYFTVN ISSPNTPGLR 190 200 210 220 230 240 NLQARDSLRE LLSRVLEARN EEGKMCTLKR PVFLKIAPDL ANEELDDIAA EATEQKLDGI 250 260 270 280 290 300 IISNTTLSRA GLKSAGGRDE TGGLSGAPLF DRSTIVLARM RERVGPDMPL IGVGGVDSAE 310 320 TALTKVKAGA RSRPALYGPD LSRPEPAY

References

[1]	"Sequence of the URA1 gene encoding dihydroorotate dehydrogenase from the basidiomycete fungus Agrocybe aegerita." Noel T., Labarere J. Gene 122:233-234(1992) [PubMed: 1452035] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: WT-2 / G30.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M90295 Genomic DNA. Translation: AAA32636.1.
PIR	JN0453.
3D structure databases
HSSP	HSSP built from PDB template 1D3G based on UniProtKB Q02127.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.3.3.1. 280072.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR012135. Dihydroorotate_DH_1_2. IPR005719. Dihydroorotate_DH_2. IPR001295. Dihydroorotate_DH_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01180. DHO_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMs	TIGR01036. pyrD_sub2. 1 hit.
PROSITE	PS00911. DHODEHASE_1. 1 hit. PS00912. DHODEHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRD_AGRAE

Accession

Primary (citable) accession number: P28294

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	October 13, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)