Reviewed, UniProtKB/Swiss-Prot P28293 (CATG_MOUSE)

Names and origin

Protein names

Recommended name:
    Cathepsin G
    EC=3.4.21.20
Alternative name(s):
    Vimentin-specific protease
      Short name=VSP

Gene names

Name:

Ctsg

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This vimentin-specific protease may regulate the reorganization of vimentin filaments, occurring during cell differentiation, movement and mitosis.
Catalytic activity	Specificity similar to chymotrypsin C.
Subcellular location	Membrane. Note: Strongly associated with membranes.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Intermediate filament Membrane
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	intermediate filament Inferred from electronic annotation. Source: UniProtKB-KW membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	By similarity
	Propeptide	19 – 20	2	Activation peptide Ref.4		PRO_0000027514
	Chain	21 – 261	241	Cathepsin G		PRO_0000027515
Regions
	Domain	21 – 243	223	Peptidase S1
Sites
	Active site	64	1	Charge relay system By similarity
	Active site	108	1	Charge relay system By similarity
	Active site	201	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	71	1	N-linked (GlcNAc...) Potential
	Disulfide bond	49 ↔ 65		By similarity
	Disulfide bond	142 ↔ 207		By similarity
	Disulfide bond	172 ↔ 186		By similarity
Experimental info
	Sequence conflict	51	1	G → S AA sequence Ref.4
	Sequence conflict	56	1	E → G AA sequence Ref.4
	Sequence conflict	60	1	L → P AA sequence Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P28293-1 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: 5EFA1A6E10E1D7FC	FASTA	261	29,096
	10 20 30 40 50 60 MQPLLLLLTF ILLQGDEAGK IIGGREARPH SYPYMAFLLI QSPEGLSACG GFLVREDFVL 70 80 90 100 110 120 TAAHCLGSSI NVTLGAHNIQ MRERTQQLIT VLRAIRHPDY NPQNIRNDIM LLQLRRRARR 130 140 150 160 170 180 SGSVKPVALP QASKKLQPGD LCTVAGWGRV SQSRGTNVLQ EVQLRVQMDQ MCANRFQFYN 190 200 210 220 230 240 SQTQICVGNP RERKSAFRGD SGGPLVCSNV AQGIVSYGSN NGNPPAVFTK IQSFMPWIKR 250 260 TMRRFAPRYQ RPANSLSQAQ T

References

[1]	"Molecular cloning, chromosomal location, and tissue-specific expression of the murine cathepsin G gene." Heusel J.W., Scarpati E.M., Jenkins N.A., Gilbert D.J., Copeland N.G., Shapiro S.D., Ley T.J. Blood 81:1614-1623(1993) [PubMed: 8453108] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Swiss Webster. Tissue: Embryonic fibroblast.
[2]	Kulmburg P., Baumruker T., Werner F. Submitted (DEC-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE.
[3]	Huang R., Aveskogh M., Hellman L.T. Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: Leaden X A1.
[4]	"Purification and characterization of a vimentin-specific protease in mouse myeloid leukemia cells. Regulation during differentiation and identity with cathepsin G." Nakamura N., Tsuru A., Hirayoshi K., Nagata K. Eur. J. Biochem. 205:947-954(1992) [PubMed: 1577012] [Abstract] Cited for: PROTEIN SEQUENCE OF 21-60.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M96801 Genomic DNA. Translation: AAA37376.1. X70057 Genomic DNA. Translation: CAA49661.1. X78544 mRNA. Translation: CAA55290.1.
IPI	IPI00118696.
PIR	S40162.
RefSeq	NP_031826.1.
UniGene	Mm.4858
3D structure databases
HSSP	HSSP built from PDB template 1EZX based on UniProtKB P00760.
SMR	P28293. Positions 21-243.
ModBase	Search...
Genome annotation databases
Ensembl	ENSMUSG00000040314. Mus musculus. [Contig view]
GeneID	13035.
KEGG	mmu:13035.
Organism-specific databases
MGI	MGI:88563. Ctsg.
Phylogenomic databases
HOGENOM	P28293.
HOVERGEN	P28293.
OMA	P28293. ICVGDRR.
Enzyme and pathway databases
BRENDA	3.4.21.20. 244.
Gene expression databases
ArrayExpress	P28293.
Bgee	P28293.
CleanEx	MM_CTSG.
GermOnline	ENSMUSG00000040314. Mus musculus.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	282916.
SOURCE	Search...

Entry information

Entry name

CATG_MOUSE

Accession

Primary (citable) accession number: P28293

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)