Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cathepsin G

Gene

Ctsg

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This vimentin-specific protease may regulate the reorganization of vimentin filaments, occurring during cell differentiation, movement and mitosis.

Catalytic activityi

Specificity similar to chymotrypsin C.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei64 – 641Charge relay systemBy similarity
Active sitei108 – 1081Charge relay systemBy similarity
Active sitei201 – 2011Charge relay systemBy similarity

GO - Molecular functioni

  1. heparin binding Source: MGI
  2. peptidase activity Source: MGI
  3. serine-type endopeptidase activity Source: MGI

GO - Biological processi

  1. defense response to fungus Source: MGI
  2. defense response to Gram-positive bacterium Source: MGI
  3. negative regulation of growth of symbiont in host Source: MGI
  4. neutrophil mediated killing of gram-positive bacterium Source: MGI
  5. positive regulation of immune response Source: MGI
  6. protein processing Source: GO_Central
  7. proteolysis Source: MGI
  8. response to lipopolysaccharide Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

ReactomeiREACT_196539. Metabolism of Angiotensinogen to Angiotensins.
REACT_199000. Activation of Matrix Metalloproteinases.
REACT_199052. Degradation of the extracellular matrix.
REACT_235886. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).

Protein family/group databases

MEROPSiS01.133.

Names & Taxonomyi

Protein namesi
Recommended name:
Cathepsin G (EC:3.4.21.20)
Alternative name(s):
Vimentin-specific protease
Short name:
VSP
Gene namesi
Name:Ctsg
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 14

Organism-specific databases

MGIiMGI:88563. Ctsg.

Subcellular locationi

Membrane
Note: Strongly associated with membranes.

GO - Cellular componenti

  1. cytoplasm Source: GO_Central
  2. extracellular matrix Source: Ensembl
  3. extracellular space Source: MGI
  4. extracellular vesicular exosome Source: MGI
  5. intermediate filament Source: UniProtKB-KW
  6. intracellular membrane-bounded organelle Source: GO_Central
  7. nucleus Source: MGI
  8. plasma membrane Source: MGI
  9. secretory granule Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Intermediate filament, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818By similarityAdd
BLAST
Propeptidei19 – 202Activation peptide1 PublicationPRO_0000027514
Chaini21 – 261241Cathepsin GPRO_0000027515Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi49 ↔ 65PROSITE-ProRule annotation
Glycosylationi71 – 711N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi142 ↔ 207PROSITE-ProRule annotation
Disulfide bondi172 ↔ 186PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PRIDEiP28293.

PTM databases

PhosphoSiteiP28293.

Expressioni

Gene expression databases

BgeeiP28293.
CleanExiMM_CTSG.
ExpressionAtlasiP28293. baseline and differential.
GenevestigatoriP28293.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000015583.

Structurei

3D structure databases

ProteinModelPortaliP28293.
SMRiP28293. Positions 21-243.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 243223Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP28293.
KOiK01319.
OMAiNIRNDIM.
OrthoDBiEOG7RRF7Z.
PhylomeDBiP28293.
TreeFamiTF333630.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28293-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQPLLLLLTF ILLQGDEAGK IIGGREARPH SYPYMAFLLI QSPEGLSACG
60 70 80 90 100
GFLVREDFVL TAAHCLGSSI NVTLGAHNIQ MRERTQQLIT VLRAIRHPDY
110 120 130 140 150
NPQNIRNDIM LLQLRRRARR SGSVKPVALP QASKKLQPGD LCTVAGWGRV
160 170 180 190 200
SQSRGTNVLQ EVQLRVQMDQ MCANRFQFYN SQTQICVGNP RERKSAFRGD
210 220 230 240 250
SGGPLVCSNV AQGIVSYGSN NGNPPAVFTK IQSFMPWIKR TMRRFAPRYQ
260
RPANSLSQAQ T
Length:261
Mass (Da):29,096
Last modified:October 1, 1996 - v2
Checksum:i5EFA1A6E10E1D7FC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti51 – 511G → S AA sequence (PubMed:1577012).Curated
Sequence conflicti56 – 561E → G AA sequence (PubMed:1577012).Curated
Sequence conflicti60 – 601L → P AA sequence (PubMed:1577012).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96801 Genomic DNA. Translation: AAA37376.1.
X70057 Genomic DNA. Translation: CAA49661.1.
X78544 mRNA. Translation: CAA55290.1.
CCDSiCCDS27142.1.
PIRiS40162.
RefSeqiNP_031826.1. NM_007800.1.
UniGeneiMm.4858.

Genome annotation databases

EnsembliENSMUST00000015583; ENSMUSP00000015583; ENSMUSG00000040314.
GeneIDi13035.
KEGGimmu:13035.
UCSCiuc007ubn.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96801 Genomic DNA. Translation: AAA37376.1.
X70057 Genomic DNA. Translation: CAA49661.1.
X78544 mRNA. Translation: CAA55290.1.
CCDSiCCDS27142.1.
PIRiS40162.
RefSeqiNP_031826.1. NM_007800.1.
UniGeneiMm.4858.

3D structure databases

ProteinModelPortaliP28293.
SMRiP28293. Positions 21-243.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000015583.

Chemistry

BindingDBiP28293.
ChEMBLiCHEMBL5622.

Protein family/group databases

MEROPSiS01.133.

PTM databases

PhosphoSiteiP28293.

Proteomic databases

PRIDEiP28293.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000015583; ENSMUSP00000015583; ENSMUSG00000040314.
GeneIDi13035.
KEGGimmu:13035.
UCSCiuc007ubn.1. mouse.

Organism-specific databases

CTDi1511.
MGIiMGI:88563. Ctsg.

Phylogenomic databases

eggNOGiCOG5640.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP28293.
KOiK01319.
OMAiNIRNDIM.
OrthoDBiEOG7RRF7Z.
PhylomeDBiP28293.
TreeFamiTF333630.

Enzyme and pathway databases

ReactomeiREACT_196539. Metabolism of Angiotensinogen to Angiotensins.
REACT_199000. Activation of Matrix Metalloproteinases.
REACT_199052. Degradation of the extracellular matrix.
REACT_235886. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).

Miscellaneous databases

NextBioi282916.
PROiP28293.
SOURCEiSearch...

Gene expression databases

BgeeiP28293.
CleanExiMM_CTSG.
ExpressionAtlasiP28293. baseline and differential.
GenevestigatoriP28293.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning, chromosomal location, and tissue-specific expression of the murine cathepsin G gene."
    Heusel J.W., Scarpati E.M., Jenkins N.A., Gilbert D.J., Copeland N.G., Shapiro S.D., Ley T.J.
    Blood 81:1614-1623(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Swiss Webster.
    Tissue: Embryonic fibroblast.
  2. Kulmburg P., Baumruker T., Werner F.
    Submitted (DEC-1992) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
  3. Huang R., Aveskogh M., Hellman L.T.
    Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: Leaden X A1.
  4. "Purification and characterization of a vimentin-specific protease in mouse myeloid leukemia cells. Regulation during differentiation and identity with cathepsin G."
    Nakamura N., Tsuru A., Hirayoshi K., Nagata K.
    Eur. J. Biochem. 205:947-954(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 21-60.

Entry informationi

Entry nameiCATG_MOUSE
AccessioniPrimary (citable) accession number: P28293
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: October 1, 1996
Last modified: March 4, 2015
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.