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Reviewed, UniProtKB/Swiss-Prot P28293 (CATG_MOUSE)

Last modified June 16, 2009. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Cathepsin G
    EC=3.4.21.20
Alternative name(s):
    Vimentin-specific protease
      Short name=VSP
Gene names
Name: Ctsg
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMus

Protein attributes

Sequence length261 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

This vimentin-specific protease may regulate the reorganization of vimentin filaments, occurring during cell differentiation, movement and mitosis.

Catalytic activity

Specificity similar to chymotrypsin C.

Subcellular location

Membrane. Note: Strongly associated with membranes.

Sequence similarities

Belongs to the peptidase S1 family.

Contains 1 peptidase S1 domain.

Ontologies

Keywords
   Cellular componentIntermediate filament
Membrane
   DomainSignal
   Molecular functionHydrolase
Protease
Serine protease
   PTMDisulfide bond
Glycoprotein
Zymogen
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological processproteolysis

Inferred from electronic annotation. Source: InterPro

   Cellular componentintermediate filament

Inferred from electronic annotation. Source: UniProtKB-KW

membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionserine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 By similarity
Propeptide19 – 202Activation peptide Ref.4
PRO_0000027514
Chain21 – 261241Cathepsin G
PRO_0000027515

Regions

Domain21 – 243223Peptidase S1

Sites

Active site641Charge relay system By similarity
Active site1081Charge relay system By similarity
Active site2011Charge relay system By similarity

Amino acid modifications

Glycosylation711N-linked (GlcNAc...) Potential
Disulfide bond49 ↔ 65 By similarity
Disulfide bond142 ↔ 207 By similarity
Disulfide bond172 ↔ 186 By similarity

Experimental info

Sequence conflict511G → S AA sequence Ref.4
Sequence conflict561E → G AA sequence Ref.4
Sequence conflict601L → P AA sequence Ref.4

Sequences

Sequence LengthMass (Da)Tools
P28293-1 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: 5EFA1A6E10E1D7FC

FASTA26129,096
        10         20         30         40         50         60 
MQPLLLLLTF ILLQGDEAGK IIGGREARPH SYPYMAFLLI QSPEGLSACG GFLVREDFVL 

        70         80         90        100        110        120 
TAAHCLGSSI NVTLGAHNIQ MRERTQQLIT VLRAIRHPDY NPQNIRNDIM LLQLRRRARR 

       130        140        150        160        170        180 
SGSVKPVALP QASKKLQPGD LCTVAGWGRV SQSRGTNVLQ EVQLRVQMDQ MCANRFQFYN 

       190        200        210        220        230        240 
SQTQICVGNP RERKSAFRGD SGGPLVCSNV AQGIVSYGSN NGNPPAVFTK IQSFMPWIKR 

       250        260 
TMRRFAPRYQ RPANSLSQAQ T 

« Hide

References

[1]"Molecular cloning, chromosomal location, and tissue-specific expression of the murine cathepsin G gene."
Heusel J.W., Scarpati E.M., Jenkins N.A., Gilbert D.J., Copeland N.G., Shapiro S.D., Ley T.J.
Blood 81:1614-1623(1993) [PubMed: 8453108] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Swiss Webster.
Tissue: Embryonic fibroblast.
[2]Kulmburg P., Baumruker T., Werner F.
Submitted (DEC-1992) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
[3]Huang R., Aveskogh M., Hellman L.T.
Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: Leaden X A1.
[4]"Purification and characterization of a vimentin-specific protease in mouse myeloid leukemia cells. Regulation during differentiation and identity with cathepsin G."
Nakamura N., Tsuru A., Hirayoshi K., Nagata K.
Eur. J. Biochem. 205:947-954(1992) [PubMed: 1577012] [Abstract]
Cited for: PROTEIN SEQUENCE OF 21-60.
+Additional computationally mapped references.

Cross-references

Sequence databases

M96801 Genomic DNA. Translation: AAA37376.1.
X70057 Genomic DNA. Translation: CAA49661.1.
X78544 mRNA. Translation: CAA55290.1.
IPIIPI00118696.
PIRS40162.
RefSeqNP_031826.1.
UniGeneMm.4858

3D structure databases

HSSPHSSP built from PDB template 1EZX based on UniProtKB P00760.
SMRP28293. Positions 21-243.
ModBaseSearch...

Genome annotation databases

EnsemblENSMUSG00000040314. Mus musculus. [Contig view]
GeneID13035.
KEGGmmu:13035.

Organism-specific databases

MGIMGI:88563. Ctsg.

Phylogenomic databases

HOGENOMP28293.
HOVERGENP28293.
OMAP28293. ICVGDRR.

Enzyme and pathway databases

BRENDA3.4.21.20. 244.

Gene expression databases

ArrayExpressP28293.
BgeeP28293.
CleanExMM_CTSG.
GermOnlineENSMUSG00000040314. Mus musculus.

Family and domain databases

InterProIPR018114. Peptidase_S1/S6_AS.
IPR001254. Peptidase_S1_S6.
IPR001314. Peptidase_S1A.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio282916.
SOURCESearch...

Entry information

Entry nameCATG_MOUSE
AccessionPrimary (citable) accession number: P28293
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: October 1, 1996
Last modified: June 16, 2009
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents