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P28293 (CATG_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cathepsin G

EC=3.4.21.20
Alternative name(s):
Vimentin-specific protease
Short name=VSP
Gene names
Name:Ctsg
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length261 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This vimentin-specific protease may regulate the reorganization of vimentin filaments, occurring during cell differentiation, movement and mitosis.

Catalytic activity

Specificity similar to chymotrypsin C.

Subcellular location

Membrane. Note: Strongly associated with membranes.

Sequence similarities

Belongs to the peptidase S1 family.

Contains 1 peptidase S1 domain.

Ontologies

Keywords
   Cellular componentIntermediate filament
Membrane
   DomainSignal
   Molecular functionHydrolase
Protease
Serine protease
   PTMDisulfide bond
Glycoprotein
Zymogen
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processdefense response to Gram-positive bacterium

Inferred from mutant phenotype PubMed 11907569. Source: MGI

defense response to fungus

Inferred from mutant phenotype PubMed 10714686. Source: MGI

negative regulation of growth of symbiont in host

Inferred from mutant phenotype PubMed 10714686. Source: MGI

neutrophil mediated killing of gram-positive bacterium

Inferred from mutant phenotype PubMed 11907569. Source: MGI

positive regulation of immune response

Inferred from mutant phenotype PubMed 10714686. Source: MGI

proteolysis

Inferred from Biological aspect of Ancestor. Source: RefGenome

response to lipopolysaccharide

Inferred from mutant phenotype PubMed 10714686. Source: MGI

   Cellular_componentextracellular matrix

Inferred from electronic annotation. Source: Ensembl

extracellular space

Inferred from electronic annotation. Source: Ensembl

intermediate filament

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: Ensembl

secretory granule

Inferred from Biological aspect of Ancestor. Source: RefGenome

   Molecular_functionheparin binding

Inferred from electronic annotation. Source: Ensembl

serine-type endopeptidase activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 By similarity
Propeptide19 – 202Activation peptide
PRO_0000027514
Chain21 – 261241Cathepsin G
PRO_0000027515

Regions

Domain21 – 243223Peptidase S1

Sites

Active site641Charge relay system By similarity
Active site1081Charge relay system By similarity
Active site2011Charge relay system By similarity

Amino acid modifications

Glycosylation711N-linked (GlcNAc...) Potential
Disulfide bond49 ↔ 65 By similarity
Disulfide bond142 ↔ 207 By similarity
Disulfide bond172 ↔ 186 By similarity

Experimental info

Sequence conflict511G → S AA sequence Ref.4
Sequence conflict561E → G AA sequence Ref.4
Sequence conflict601L → P AA sequence Ref.4

Sequences

Sequence LengthMass (Da)Tools
P28293 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: 5EFA1A6E10E1D7FC

FASTA26129,096
        10         20         30         40         50         60 
MQPLLLLLTF ILLQGDEAGK IIGGREARPH SYPYMAFLLI QSPEGLSACG GFLVREDFVL 

        70         80         90        100        110        120 
TAAHCLGSSI NVTLGAHNIQ MRERTQQLIT VLRAIRHPDY NPQNIRNDIM LLQLRRRARR 

       130        140        150        160        170        180 
SGSVKPVALP QASKKLQPGD LCTVAGWGRV SQSRGTNVLQ EVQLRVQMDQ MCANRFQFYN 

       190        200        210        220        230        240 
SQTQICVGNP RERKSAFRGD SGGPLVCSNV AQGIVSYGSN NGNPPAVFTK IQSFMPWIKR 

       250        260 
TMRRFAPRYQ RPANSLSQAQ T 

« Hide

References

[1]"Molecular cloning, chromosomal location, and tissue-specific expression of the murine cathepsin G gene."
Heusel J.W., Scarpati E.M., Jenkins N.A., Gilbert D.J., Copeland N.G., Shapiro S.D., Ley T.J.
Blood 81:1614-1623(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Swiss Webster.
Tissue: Embryonic fibroblast.
[2]Kulmburg P., Baumruker T., Werner F.
Submitted (DEC-1992) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
[3]Huang R., Aveskogh M., Hellman L.T.
Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: Leaden X A1.
[4]"Purification and characterization of a vimentin-specific protease in mouse myeloid leukemia cells. Regulation during differentiation and identity with cathepsin G."
Nakamura N., Tsuru A., Hirayoshi K., Nagata K.
Eur. J. Biochem. 205:947-954(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 21-60.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M96801 Genomic DNA. Translation: AAA37376.1.
X70057 Genomic DNA. Translation: CAA49661.1.
X78544 mRNA. Translation: CAA55290.1.
PIRS40162.
RefSeqNP_031826.1. NM_007800.1.
UniGeneMm.4858.

3D structure databases

ProteinModelPortalP28293.
SMRP28293. Positions 21-243.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000015583.

Chemistry

BindingDBP28293.
ChEMBLCHEMBL5622.

Protein family/group databases

MEROPSS01.133.

PTM databases

PhosphoSiteP28293.

Proteomic databases

PRIDEP28293.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000015583; ENSMUSP00000015583; ENSMUSG00000040314.
GeneID13035.
KEGGmmu:13035.
UCSCuc007ubn.1. mouse.

Organism-specific databases

CTD1511.
MGIMGI:88563. Ctsg.

Phylogenomic databases

eggNOGCOG5640.
HOGENOMHOG000251820.
HOVERGENHBG013304.
InParanoidP28293.
KOK01319.
OMASQTQICV.
OrthoDBEOG7RRF7Z.
PhylomeDBP28293.
TreeFamTF333630.

Gene expression databases

ArrayExpressP28293.
BgeeP28293.
CleanExMM_CTSG.
GenevestigatorP28293.

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio282916.
PROP28293.
SOURCESearch...

Entry information

Entry nameCATG_MOUSE
AccessionPrimary (citable) accession number: P28293
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot