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P28293

- CATG_MOUSE

UniProt

P28293 - CATG_MOUSE

Protein

Cathepsin G

Gene

Ctsg

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 2 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    This vimentin-specific protease may regulate the reorganization of vimentin filaments, occurring during cell differentiation, movement and mitosis.

    Catalytic activityi

    Specificity similar to chymotrypsin C.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei64 – 641Charge relay systemBy similarity
    Active sitei108 – 1081Charge relay systemBy similarity
    Active sitei201 – 2011Charge relay systemBy similarity

    GO - Molecular functioni

    1. heparin binding Source: Ensembl
    2. serine-type endopeptidase activity Source: RefGenome

    GO - Biological processi

    1. defense response to fungus Source: MGI
    2. defense response to Gram-positive bacterium Source: MGI
    3. negative regulation of growth of symbiont in host Source: MGI
    4. neutrophil mediated killing of gram-positive bacterium Source: MGI
    5. positive regulation of immune response Source: MGI
    6. proteolysis Source: RefGenome
    7. response to lipopolysaccharide Source: MGI

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Enzyme and pathway databases

    ReactomeiREACT_196539. Metabolism of Angiotensinogen to Angiotensins.
    REACT_199000. Activation of Matrix Metalloproteinases.
    REACT_199052. Degradation of the extracellular matrix.

    Protein family/group databases

    MEROPSiS01.133.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cathepsin G (EC:3.4.21.20)
    Alternative name(s):
    Vimentin-specific protease
    Short name:
    VSP
    Gene namesi
    Name:Ctsg
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 14

    Organism-specific databases

    MGIiMGI:88563. Ctsg.

    Subcellular locationi

    Membrane
    Note: Strongly associated with membranes.

    GO - Cellular componenti

    1. extracellular matrix Source: Ensembl
    2. extracellular space Source: Ensembl
    3. intermediate filament Source: UniProtKB-KW
    4. plasma membrane Source: Ensembl
    5. secretory granule Source: RefGenome

    Keywords - Cellular componenti

    Intermediate filament, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818By similarityAdd
    BLAST
    Propeptidei19 – 202Activation peptide1 PublicationPRO_0000027514
    Chaini21 – 261241Cathepsin GPRO_0000027515Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi49 ↔ 65PROSITE-ProRule annotation
    Glycosylationi71 – 711N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi142 ↔ 207PROSITE-ProRule annotation
    Disulfide bondi172 ↔ 186PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Zymogen

    Proteomic databases

    PRIDEiP28293.

    PTM databases

    PhosphoSiteiP28293.

    Expressioni

    Gene expression databases

    ArrayExpressiP28293.
    BgeeiP28293.
    CleanExiMM_CTSG.
    GenevestigatoriP28293.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000015583.

    Structurei

    3D structure databases

    ProteinModelPortaliP28293.
    SMRiP28293. Positions 21-243.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini21 – 243223Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5640.
    HOGENOMiHOG000251820.
    HOVERGENiHBG013304.
    InParanoidiP28293.
    KOiK01319.
    OMAiNIRNDIM.
    OrthoDBiEOG7RRF7Z.
    PhylomeDBiP28293.
    TreeFamiTF333630.

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P28293-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQPLLLLLTF ILLQGDEAGK IIGGREARPH SYPYMAFLLI QSPEGLSACG    50
    GFLVREDFVL TAAHCLGSSI NVTLGAHNIQ MRERTQQLIT VLRAIRHPDY 100
    NPQNIRNDIM LLQLRRRARR SGSVKPVALP QASKKLQPGD LCTVAGWGRV 150
    SQSRGTNVLQ EVQLRVQMDQ MCANRFQFYN SQTQICVGNP RERKSAFRGD 200
    SGGPLVCSNV AQGIVSYGSN NGNPPAVFTK IQSFMPWIKR TMRRFAPRYQ 250
    RPANSLSQAQ T 261
    Length:261
    Mass (Da):29,096
    Last modified:October 1, 1996 - v2
    Checksum:i5EFA1A6E10E1D7FC
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti51 – 511G → S AA sequence (PubMed:1577012)Curated
    Sequence conflicti56 – 561E → G AA sequence (PubMed:1577012)Curated
    Sequence conflicti60 – 601L → P AA sequence (PubMed:1577012)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M96801 Genomic DNA. Translation: AAA37376.1.
    X70057 Genomic DNA. Translation: CAA49661.1.
    X78544 mRNA. Translation: CAA55290.1.
    CCDSiCCDS27142.1.
    PIRiS40162.
    RefSeqiNP_031826.1. NM_007800.1.
    UniGeneiMm.4858.

    Genome annotation databases

    EnsembliENSMUST00000015583; ENSMUSP00000015583; ENSMUSG00000040314.
    GeneIDi13035.
    KEGGimmu:13035.
    UCSCiuc007ubn.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M96801 Genomic DNA. Translation: AAA37376.1 .
    X70057 Genomic DNA. Translation: CAA49661.1 .
    X78544 mRNA. Translation: CAA55290.1 .
    CCDSi CCDS27142.1.
    PIRi S40162.
    RefSeqi NP_031826.1. NM_007800.1.
    UniGenei Mm.4858.

    3D structure databases

    ProteinModelPortali P28293.
    SMRi P28293. Positions 21-243.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000015583.

    Chemistry

    BindingDBi P28293.
    ChEMBLi CHEMBL5622.

    Protein family/group databases

    MEROPSi S01.133.

    PTM databases

    PhosphoSitei P28293.

    Proteomic databases

    PRIDEi P28293.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000015583 ; ENSMUSP00000015583 ; ENSMUSG00000040314 .
    GeneIDi 13035.
    KEGGi mmu:13035.
    UCSCi uc007ubn.1. mouse.

    Organism-specific databases

    CTDi 1511.
    MGIi MGI:88563. Ctsg.

    Phylogenomic databases

    eggNOGi COG5640.
    HOGENOMi HOG000251820.
    HOVERGENi HBG013304.
    InParanoidi P28293.
    KOi K01319.
    OMAi NIRNDIM.
    OrthoDBi EOG7RRF7Z.
    PhylomeDBi P28293.
    TreeFami TF333630.

    Enzyme and pathway databases

    Reactomei REACT_196539. Metabolism of Angiotensinogen to Angiotensins.
    REACT_199000. Activation of Matrix Metalloproteinases.
    REACT_199052. Degradation of the extracellular matrix.

    Miscellaneous databases

    NextBioi 282916.
    PROi P28293.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P28293.
    Bgeei P28293.
    CleanExi MM_CTSG.
    Genevestigatori P28293.

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning, chromosomal location, and tissue-specific expression of the murine cathepsin G gene."
      Heusel J.W., Scarpati E.M., Jenkins N.A., Gilbert D.J., Copeland N.G., Shapiro S.D., Ley T.J.
      Blood 81:1614-1623(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Swiss Webster.
      Tissue: Embryonic fibroblast.
    2. Kulmburg P., Baumruker T., Werner F.
      Submitted (DEC-1992) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
    3. Huang R., Aveskogh M., Hellman L.T.
      Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: Leaden X A1.
    4. "Purification and characterization of a vimentin-specific protease in mouse myeloid leukemia cells. Regulation during differentiation and identity with cathepsin G."
      Nakamura N., Tsuru A., Hirayoshi K., Nagata K.
      Eur. J. Biochem. 205:947-954(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 21-60.

    Entry informationi

    Entry nameiCATG_MOUSE
    AccessioniPrimary (citable) accession number: P28293
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 115 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3