ID   CCL2_BOVIN              Reviewed;          99 AA.
AC   P28291; A9QWP7;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-1992, sequence version 1.
DT   24-JAN-2024, entry version 143.
DE   RecName: Full=C-C motif chemokine 2;
DE   AltName: Full=Acidic seminal fluid protein;
DE   AltName: Full=Monocyte chemotactic protein 1A;
DE            Short=MCP-1;
DE            Short=MCP-1A;
DE   AltName: Full=Small-inducible cytokine A2;
DE   Flags: Precursor;
GN   Name=CCL2; Synonyms=SCYA2;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Seminal plasma;
RX   PubMed=1721821; DOI=10.1089/dna.1991.10.671;
RA   Wempe F., Henschen A., Scheit K.H.;
RT   "Gene expression and cDNA cloning identified a major basic protein
RT   constituent of bovine seminal plasma as bovine monocyte-chemoattractant
RT   protein-1 (MCP-1).";
RL   DNA Cell Biol. 10:671-679(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Seminal plasma;
RX   PubMed=1543494; DOI=10.1016/0006-291x(92)91633-2;
RA   Wempe F., Einspanier R., Scheit K.H.;
RT   "Characterization by cDNA cloning of the mRNA of a new growth factor from
RT   bovine seminal plasma: acidic seminal fluid protein.";
RL   Biochem. Biophys. Res. Commun. 183:232-237(1992).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8060303; DOI=10.1006/bbrc.1994.2068;
RA   Wempe F., Kuhlmann J.K., Scheit K.H.;
RT   "Characterization of the bovine monocyte chemoattractant protein-1 gene.";
RL   Biochem. Biophys. Res. Commun. 202:1272-1279(1994).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Belted Galloway; TISSUE=Peripheral blood;
RG   U.S. Veterinary Immune Reagent Network;
RA   Hudgens T., Tompkins D., Baldwin C.L.;
RT   "U.S. veterinary immune reagent network: expressed bovine gene sequences.";
RL   Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Acts as a ligand for C-C chemokine receptor CCR2 (By
CC       similarity). Signals through binding and activation of CCR2 and induces
CC       a strong chemotactic response and mobilization of intracellular calcium
CC       ions (By similarity). Exhibits a chemotactic activity for monocytes and
CC       basophils but not neutrophils or eosinophils (By similarity). Plays an
CC       important role in mediating peripheral nerve injury-induced neuropathic
CC       pain (By similarity). Increases NMDA-mediated synaptic transmission in
CC       both dopamine D1 and D2 receptor-containing neurons, which may be
CC       caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B (By
CC       similarity). {ECO:0000250|UniProtKB:P10148,
CC       ECO:0000250|UniProtKB:P13500}.
CC   -!- SUBUNIT: Monomer or homodimer; in equilibrium. Is tethered on
CC       endothelial cells by glycosaminoglycan (GAG) side chains of
CC       proteoglycans. Interacts with TNFAIP6 (via Link domain).
CC       {ECO:0000250|UniProtKB:P13500}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P13500}.
CC   -!- PTM: Processing at the N-terminus can regulate receptor and target cell
CC       selectivity (By similarity). Deletion of the N-terminal residue
CC       converts it from an activator of basophil to an eosinophil
CC       chemoattractant (By similarity). {ECO:0000250|UniProtKB:P13500}.
CC   -!- PTM: N-Glycosylated. {ECO:0000250|UniProtKB:P13500}.
CC   -!- SIMILARITY: Belongs to the intercrine beta (chemokine CC) family.
CC       {ECO:0000305}.
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DR   EMBL; M84602; AAA30651.1; -; mRNA.
DR   EMBL; L32659; AAA60956.1; -; Genomic_DNA.
DR   EMBL; EU276059; ABX72057.1; -; mRNA.
DR   PIR; A39296; A39296.
DR   RefSeq; NP_776431.1; NM_174006.2.
DR   AlphaFoldDB; P28291; -.
DR   SMR; P28291; -.
DR   STRING; 9913.ENSBTAP00000013146; -.
DR   PaxDb; 9913-ENSBTAP00000013146; -.
DR   Ensembl; ENSBTAT00000013146.6; ENSBTAP00000013146.5; ENSBTAG00000037811.3.
DR   GeneID; 281043; -.
DR   KEGG; bta:281043; -.
DR   CTD; 6347; -.
DR   VEuPathDB; HostDB:ENSBTAG00000037811; -.
DR   eggNOG; ENOG502S6ZP; Eukaryota.
DR   GeneTree; ENSGT01100000263482; -.
DR   HOGENOM; CLU_141716_1_0_1; -.
DR   InParanoid; P28291; -.
DR   OMA; PITCCYT; -.
DR   OrthoDB; 4265193at2759; -.
DR   TreeFam; TF334888; -.
DR   Reactome; R-BTA-380108; Chemokine receptors bind chemokines.
DR   Proteomes; UP000009136; Chromosome 19.
DR   Bgee; ENSBTAG00000037811; Expressed in intramuscular adipose tissue and 98 other cell types or tissues.
DR   GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR   GO; GO:0048020; F:CCR chemokine receptor binding; IBA:GO_Central.
DR   GO; GO:0008009; F:chemokine activity; IBA:GO_Central.
DR   GO; GO:0043615; P:astrocyte cell migration; IEA:Ensembl.
DR   GO; GO:0044344; P:cellular response to fibroblast growth factor stimulus; IEA:Ensembl.
DR   GO; GO:0071347; P:cellular response to interleukin-1; IBA:GO_Central.
DR   GO; GO:0071356; P:cellular response to tumor necrosis factor; IBA:GO_Central.
DR   GO; GO:0071346; P:cellular response to type II interferon; IBA:GO_Central.
DR   GO; GO:0070098; P:chemokine-mediated signaling pathway; IBA:GO_Central.
DR   GO; GO:0007010; P:cytoskeleton organization; IEA:Ensembl.
DR   GO; GO:0048245; P:eosinophil chemotaxis; IBA:GO_Central.
DR   GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR   GO; GO:0006954; P:inflammatory response; IBA:GO_Central.
DR   GO; GO:0048247; P:lymphocyte chemotaxis; IBA:GO_Central.
DR   GO; GO:0048246; P:macrophage chemotaxis; IEA:Ensembl.
DR   GO; GO:0002548; P:monocyte chemotaxis; IBA:GO_Central.
DR   GO; GO:2000134; P:negative regulation of G1/S transition of mitotic cell cycle; IEA:Ensembl.
DR   GO; GO:0034351; P:negative regulation of glial cell apoptotic process; IEA:Ensembl.
DR   GO; GO:2000502; P:negative regulation of natural killer cell chemotaxis; IEA:Ensembl.
DR   GO; GO:0043524; P:negative regulation of neuron apoptotic process; IEA:Ensembl.
DR   GO; GO:1905563; P:negative regulation of vascular endothelial cell proliferation; IEA:Ensembl.
DR   GO; GO:0030593; P:neutrophil chemotaxis; IBA:GO_Central.
DR   GO; GO:0090280; P:positive regulation of calcium ion import; IEA:Ensembl.
DR   GO; GO:2000353; P:positive regulation of endothelial cell apoptotic process; IEA:Ensembl.
DR   GO; GO:0070374; P:positive regulation of ERK1 and ERK2 cascade; IBA:GO_Central.
DR   GO; GO:0051968; P:positive regulation of synaptic transmission, glutamatergic; ISS:UniProtKB.
DR   GO; GO:0008360; P:regulation of cell shape; IEA:Ensembl.
DR   GO; GO:0019233; P:sensory perception of pain; ISS:UniProtKB.
DR   CDD; cd00272; Chemokine_CC; 1.
DR   Gene3D; 2.40.50.40; -; 1.
DR   InterPro; IPR039809; Chemokine_b/g/d.
DR   InterPro; IPR000827; Chemokine_CC_CS.
DR   InterPro; IPR001811; Chemokine_IL8-like_dom.
DR   InterPro; IPR036048; Interleukin_8-like_sf.
DR   PANTHER; PTHR12015:SF98; C-C MOTIF CHEMOKINE 2; 1.
DR   PANTHER; PTHR12015; SMALL INDUCIBLE CYTOKINE A; 1.
DR   Pfam; PF00048; IL8; 1.
DR   SMART; SM00199; SCY; 1.
DR   SUPFAM; SSF54117; Interleukin 8-like chemokines; 1.
DR   PROSITE; PS00472; SMALL_CYTOKINES_CC; 1.
PE   3: Inferred from homology;
KW   Chemotaxis; Cytokine; Disulfide bond; Inflammatory response;
KW   Pyrrolidone carboxylic acid; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000250"
FT   CHAIN           24..99
FT                   /note="C-C motif chemokine 2"
FT                   /id="PRO_0000005244"
FT   MOD_RES         24
FT                   /note="Pyrrolidone carboxylic acid"
FT                   /evidence="ECO:0000250|UniProtKB:P13500"
FT   DISULFID        34..59
FT                   /evidence="ECO:0000250"
FT   DISULFID        35..75
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   99 AA;  11114 MW;  0FD79FC1AB0CBE88 CRC64;
     MKVSAALLCL LLTVAAFSTE VLAQPDAINS QVACCYTFNS KKISMQRLMN YRRVTSSKCP
     KEAVIFKTIL GKELCADPKQ KWVQDSINYL NKKNQTPKP
//