UniProtKB - P28274 (URA7_YEAST)
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Protein
CTP synthase 1
Gene
URA7
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen.
Miscellaneous
Present with 57600 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
ATP + UTP + L-glutamine = ADP + phosphate + CTP + L-glutamate.
Enzyme regulationi
Activated by GTP and inhibited by CTP.1 Publication
Kineticsi
- KM=60 µM for UTP1 Publication
- KM=0.9 mM for ATP1 Publication
- KM=0.4 mM for L-glutamine1 Publication
pH dependencei
Optimum pH is 8.0.1 Publication
: CTP biosynthesis via de novo pathway Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes CTP from UDP.Proteins known to be involved in the 2 steps of the subpathway in this organism are:
- no protein annotated in this organism
- CTP synthase 1 (URA7), CTP synthase 2 (URA8)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CTP from UDP, the pathway CTP biosynthesis via de novo pathway and in Pyrimidine metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 404 | For GATase activityPROSITE-ProRule annotation | 1 | |
Active sitei | 535 | For GATase activityPROSITE-ProRule annotation | 1 | |
Active sitei | 537 | For GATase activityPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- CTP synthase activity Source: SGD
GO - Biological processi
- 'de novo' CTP biosynthetic process Source: UniProtKB-UniPathway
- CTP biosynthetic process Source: SGD
- glutamine metabolic process Source: UniProtKB-KW
- phospholipid biosynthetic process Source: SGD
- pyrimidine nucleobase biosynthetic process Source: SGD
Keywordsi
Molecular function | Ligase |
Biological process | Pyrimidine biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | YEAST:YBL039C-MONOMER |
BRENDAi | 6.3.4.2 984 |
SABIO-RKi | P28274 |
UniPathwayi | UPA00159; UER00277 |
Protein family/group databases
MEROPSi | C26.A36 |
Names & Taxonomyi
Protein namesi | Recommended name: CTP synthase 1 (EC:6.3.4.2)Alternative name(s): CTP synthetase 1 UTP--ammonia ligase 1 |
Gene namesi | Name:URA7 Ordered Locus Names:YBL039C ORF Names:YBL0410 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YBL039C |
SGDi | S000000135 URA7 |
Subcellular locationi
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000138283 | 1 – 579 | CTP synthase 1Add BLAST | 579 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 422 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication |
Keywords - PTMi
Isopeptide bond, Ubl conjugationProteomic databases
MaxQBi | P28274 |
PaxDbi | P28274 |
PRIDEi | P28274 |
PTM databases
iPTMneti | P28274 |
Interactioni
Subunit structurei
Homodimer. Oligomerizes to a tetramer in the presence of its substrates UTP and ATP.1 Publication
Binary interactionsi
Protein-protein interaction databases
BioGridi | 32658243 interactors. |
DIPi | DIP-2954N |
IntActi | P28274 96 interactors. |
MINTi | P28274 |
STRINGi | 4932.YBL039C |
Structurei
3D structure databases
ProteinModelPortali | P28274 |
SMRi | P28274 |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 305 – 559 | Glutamine amidotransferase type-1PROSITE-ProRule annotationAdd BLAST | 255 |
Sequence similaritiesi
Belongs to the CTP synthase family.Curated
Keywords - Domaini
Glutamine amidotransferasePhylogenomic databases
GeneTreei | ENSGT00910000144179 |
HOGENOMi | HOG000077514 |
InParanoidi | P28274 |
KOi | K01937 |
OMAi | EFNNAYR |
OrthoDBi | EOG092C23X9 |
Family and domain databases
CDDi | cd01746 GATase1_CTP_Synthase, 1 hit |
Gene3Di | 3.40.50.8801 hit |
InterProi | View protein in InterPro IPR029062 Class_I_gatase-like IPR004468 CTP_synthase IPR017456 CTP_synthase_N IPR017926 GATASE IPR033828 GATase1_CTP_Synthase IPR027417 P-loop_NTPase |
PANTHERi | PTHR11550 PTHR11550, 1 hit |
Pfami | View protein in Pfam PF06418 CTP_synth_N, 1 hit PF00117 GATase, 1 hit |
SUPFAMi | SSF52317 SSF52317, 1 hit SSF52540 SSF52540, 1 hit |
TIGRFAMsi | TIGR00337 PyrG, 1 hit |
PROSITEi | View protein in PROSITE PS51273 GATASE_TYPE_1, 1 hit |
i Sequence
Sequence statusi: Complete.
P28274-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKYVVVSGGV ISGIGKGVLA SSTGMLMKTL GLKVTSIKID PYMNIDAGTM
60 70 80 90 100
SPLEHGECFV LDDGGETDLD LGNYERYLGV TLTKDHNITT GKIYSHVIAK
110 120 130 140 150
ERKGDYLGKT VQIVPHLTNA IQDWIERVAK IPVDDTGMEP DVCIIELGGT
160 170 180 190 200
VGDIESAPFV EALRQFQFKV GKENFALIHV SLVPVIHGEQ KTKPTQAAIK
210 220 230 240 250
GLRSLGLVPD MIACRCSETL DKPTIDKIAM FCHVGPEQVV NVHDVNSTYH
260 270 280 290 300
VPLLLLEQKM IDYLHARLKL DEISLTEEEK QRGLELLSKW KATTGNFDES
310 320 330 340 350
METVKIALVG KYTNLKDSYL SVIKALEHSS MKCRRKLDIK WVEATDLEPE
360 370 380 390 400
AQESNKTKFH EAWNMVSTAD GILIPGGFGV RGTEGMVLAA RWARENHIPF
410 420 430 440 450
LGVCLGLQIA TIEFTRSVLG RKDSHSAEFY PDIDEKNHVV VFMPEIDKET
460 470 480 490 500
MGGSMRLGLR PTFFQNETEW SQIKKLYGDV SEVHERHRHR YEINPKMVDE
510 520 530 540 550
LENNGLIFVG KDDTGKRCEI LELKNHPYYI ATQYHPEYTS KVLDPSKPFL
560 570
GLVAASAGIL QDVIEGKYDL EAGENKFNF
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 307 | A → R in CAA37941 (PubMed:1753946).Curated | 1 | |
Sequence conflicti | 418 | V → S in CAA37941 (PubMed:1753946).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X53995 Genomic DNA Translation: CAA37941.1 X78214 Genomic DNA Translation: CAA55055.1 Z35800 Genomic DNA Translation: CAA84859.1 BK006936 Genomic DNA Translation: DAA07080.1 |
PIRi | S50291 |
RefSeqi | NP_009514.1, NM_001178279.1 |
Genome annotation databases
EnsemblFungii | YBL039C; YBL039C; YBL039C |
GeneIDi | 852241 |
KEGGi | sce:YBL039C |
Similar proteinsi
Entry informationi
Entry namei | URA7_YEAST | |
Accessioni | P28274Primary (citable) accession number: P28274 Secondary accession number(s): D6VPW0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 1992 |
Last sequence update: | October 1, 1994 | |
Last modified: | March 28, 2018 | |
This is version 170 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |