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UniProtKB - P28274 (URA7_YEAST)

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Protein

CTP synthase 1

Gene

URA7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen.

Miscellaneous

Present with 57600 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + UTP + L-glutamine = ADP + phosphate + CTP + L-glutamate.

Enzyme regulationi

Activated by GTP and inhibited by CTP.1 Publication

Kineticsi

  1. KM=60 µM for UTP1 Publication
  2. KM=0.9 mM for ATP1 Publication
  3. KM=0.4 mM for L-glutamine1 Publication

    pH dependencei

    Optimum pH is 8.0.1 Publication

    Pathwayi: CTP biosynthesis via de novo pathway

    This protein is involved in step 2 of the subpathway that synthesizes CTP from UDP.
    Proteins known to be involved in the 2 steps of the subpathway in this organism are:
    1. no protein annotated in this organism
    2. CTP synthase 1 (URA7), CTP synthase 2 (URA8)
    This subpathway is part of the pathway CTP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes CTP from UDP, the pathway CTP biosynthesis via de novo pathway and in Pyrimidine metabolism.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei404For GATase activityPROSITE-ProRule annotation1
    Active sitei535For GATase activityPROSITE-ProRule annotation1
    Active sitei537For GATase activityPROSITE-ProRule annotation1

    GO - Molecular functioni

    • ATP binding Source: UniProtKB-KW
    • CTP synthase activity Source: SGD
    Complete GO annotation...

    GO - Biological processi

    • 'de novo' CTP biosynthetic process Source: UniProtKB-UniPathway
    • CTP biosynthetic process Source: SGD
    • glutamine metabolic process Source: UniProtKB-KW
    • phospholipid biosynthetic process Source: SGD
    • pyrimidine nucleobase biosynthetic process Source: SGD
    Complete GO annotation...

    Keywordsi

    Molecular functionLigase
    Biological processPyrimidine biosynthesis
    LigandATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:YBL039C-MONOMER.
    BRENDAi6.3.4.2. 984.
    ReactomeiR-SCE-499943. Interconversion of nucleotide di- and triphosphates.
    SABIO-RKiP28274.
    UniPathwayiUPA00159; UER00277.

    Protein family/group databases

    MEROPSiC26.A36.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    CTP synthase 1 (EC:6.3.4.2)
    Alternative name(s):
    CTP synthetase 1
    UTP--ammonia ligase 1
    Gene namesi
    Name:URA7
    Ordered Locus Names:YBL039C
    ORF Names:YBL0410
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›
    Proteomesi
    • UP000002311 Componenti: Chromosome II

    Organism-specific databases

    EuPathDBiFungiDB:YBL039C.
    SGDiS000000135. URA7.

    Subcellular locationi

    GO - Cellular componenti

    • cytoophidium Source: SGD
    Complete GO annotation...

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001382831 – 579CTP synthase 1Add BLAST579

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Cross-linki422Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

    Keywords - PTMi

    Isopeptide bond, Ubl conjugation

    Proteomic databases

    MaxQBiP28274.
    PRIDEiP28274.

    PTM databases

    iPTMnetiP28274.

    Interactioni

    Subunit structurei

    Homodimer. Oligomerizes to a tetramer in the presence of its substrates UTP and ATP.1 Publication

    Protein-protein interaction databases

    BioGridi32658. 242 interactors.
    DIPiDIP-2954N.
    IntActiP28274. 67 interactors.
    MINTiMINT-506283.

    Structurei

    3D structure databases

    ProteinModelPortaliP28274.
    SMRiP28274.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini305 – 559Glutamine amidotransferase type-1PROSITE-ProRule annotationAdd BLAST255

    Sequence similaritiesi

    Belongs to the CTP synthase family.Curated

    Keywords - Domaini

    Glutamine amidotransferase

    Phylogenomic databases

    GeneTreeiENSGT00880000138861.
    HOGENOMiHOG000077514.
    InParanoidiP28274.
    KOiK01937.
    OMAiEFNNAYR.
    OrthoDBiEOG092C23X9.

    Family and domain databases

    CDDicd01746. GATase1_CTP_Synthase. 1 hit.
    Gene3Di3.40.50.880. 1 hit.
    InterProiView protein in InterPro
    IPR029062. Class_I_gatase-like.
    IPR004468. CTP_synthase.
    IPR017456. CTP_synthase_N.
    IPR017926. GATASE.
    IPR033828. GATase1_CTP_Synthase.
    IPR027417. P-loop_NTPase.
    PANTHERiPTHR11550. PTHR11550. 1 hit.
    PfamiView protein in Pfam
    PF06418. CTP_synth_N. 1 hit.
    PF00117. GATase. 1 hit.
    SUPFAMiSSF52317. SSF52317. 1 hit.
    SSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00337. PyrG. 1 hit.
    PROSITEiView protein in PROSITE
    PS51273. GATASE_TYPE_1. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P28274-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKYVVVSGGV ISGIGKGVLA SSTGMLMKTL GLKVTSIKID PYMNIDAGTM 
            60         70         80         90        100
    SPLEHGECFV LDDGGETDLD LGNYERYLGV TLTKDHNITT GKIYSHVIAK 
           110        120        130        140        150
    ERKGDYLGKT VQIVPHLTNA IQDWIERVAK IPVDDTGMEP DVCIIELGGT 
           160        170        180        190        200
    VGDIESAPFV EALRQFQFKV GKENFALIHV SLVPVIHGEQ KTKPTQAAIK 
           210        220        230        240        250
    GLRSLGLVPD MIACRCSETL DKPTIDKIAM FCHVGPEQVV NVHDVNSTYH 
           260        270        280        290        300
    VPLLLLEQKM IDYLHARLKL DEISLTEEEK QRGLELLSKW KATTGNFDES 
           310        320        330        340        350
    METVKIALVG KYTNLKDSYL SVIKALEHSS MKCRRKLDIK WVEATDLEPE 
           360        370        380        390        400
    AQESNKTKFH EAWNMVSTAD GILIPGGFGV RGTEGMVLAA RWARENHIPF 
           410        420        430        440        450
    LGVCLGLQIA TIEFTRSVLG RKDSHSAEFY PDIDEKNHVV VFMPEIDKET 
           460        470        480        490        500
    MGGSMRLGLR PTFFQNETEW SQIKKLYGDV SEVHERHRHR YEINPKMVDE 
           510        520        530        540        550
    LENNGLIFVG KDDTGKRCEI LELKNHPYYI ATQYHPEYTS KVLDPSKPFL 
           560        570 
    GLVAASAGIL QDVIEGKYDL EAGENKFNF
    Length:579
    Mass (Da):64,710
    Last modified:October 1, 1994 - v2
    Checksum:iEC3766C83B22ED92
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti307A → R in CAA37941 (PubMed:1753946).Curated1
    Sequence conflicti418V → S in CAA37941 (PubMed:1753946).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    		X53995 Genomic DNA. Translation: CAA37941.1.
    X78214 Genomic DNA. Translation: CAA55055.1.
    Z35800 Genomic DNA. Translation: CAA84859.1.
    BK006936 Genomic DNA. Translation: DAA07080.1.
    PIRiS50291.
    RefSeqiNP_009514.1. NM_001178279.1.

    Genome annotation databases

    EnsemblFungiiYBL039C; YBL039C; YBL039C.
    GeneIDi852241.
    KEGGisce:YBL039C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    		X53995 Genomic DNA. Translation: CAA37941.1.
    X78214 Genomic DNA. Translation: CAA55055.1.
    Z35800 Genomic DNA. Translation: CAA84859.1.
    BK006936 Genomic DNA. Translation: DAA07080.1.
    PIRiS50291.
    RefSeqiNP_009514.1. NM_001178279.1.

    3D structure databases

    ProteinModelPortaliP28274.
    SMRiP28274.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi32658. 242 interactors.
    DIPiDIP-2954N.
    IntActiP28274. 67 interactors.
    MINTiMINT-506283.

    Protein family/group databases

    MEROPSiC26.A36.

    PTM databases

    iPTMnetiP28274.

    Proteomic databases

    MaxQBiP28274.
    PRIDEiP28274.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblFungiiYBL039C; YBL039C; YBL039C.
    GeneIDi852241.
    KEGGisce:YBL039C.

    Organism-specific databases

    EuPathDBiFungiDB:YBL039C.
    SGDiS000000135. URA7.

    Phylogenomic databases

    GeneTreeiENSGT00880000138861.
    HOGENOMiHOG000077514.
    InParanoidiP28274.
    KOiK01937.
    OMAiEFNNAYR.
    OrthoDBiEOG092C23X9.

    Enzyme and pathway databases

    UniPathwayiUPA00159; UER00277.
    BioCyciYEAST:YBL039C-MONOMER.
    BRENDAi6.3.4.2. 984.
    ReactomeiR-SCE-499943. Interconversion of nucleotide di- and triphosphates.
    SABIO-RKiP28274.

    Miscellaneous databases

    PROiPR:P28274.

    Family and domain databases

    CDDicd01746. GATase1_CTP_Synthase. 1 hit.
    Gene3Di3.40.50.880. 1 hit.
    InterProiView protein in InterPro
    IPR029062. Class_I_gatase-like.
    IPR004468. CTP_synthase.
    IPR017456. CTP_synthase_N.
    IPR017926. GATASE.
    IPR033828. GATase1_CTP_Synthase.
    IPR027417. P-loop_NTPase.
    PANTHERiPTHR11550. PTHR11550. 1 hit.
    PfamiView protein in Pfam
    PF06418. CTP_synth_N. 1 hit.
    PF00117. GATase. 1 hit.
    SUPFAMiSSF52317. SSF52317. 1 hit.
    SSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00337. PyrG. 1 hit.
    PROSITEiView protein in PROSITE
    PS51273. GATASE_TYPE_1. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiURA7_YEAST
    AccessioniPrimary (citable) accession number: P28274
    Secondary accession number(s): D6VPW0
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: October 1, 1994
    Last modified: June 7, 2017
    This is version 162 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.