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Protein

5-oxoprolinase

Gene

OXP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the cleavage of 5-oxo-L-proline to form L-glutamate coupled to the hydrolysis of ATP to ADP and inorganic phosphate.2 Publications

Catalytic activityi

ATP + 5-oxo-L-proline + 2 H2O = ADP + phosphate + L-glutamate.

Kineticsi

  1. KM=159 µM for 5-oxoproline1 Publication
  1. Vmax=3.5 nmol/h/µg enzyme1 Publication

GO - Molecular functioni

  • 5-oxoprolinase (ATP-hydrolyzing) activity Source: SGD
  • ATP binding Source: UniProtKB-KW

GO - Biological processi

  • glutathione metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-6163-MONOMER.
YEAST:G3O-31973-MONOMER.
BRENDAi3.5.2.9. 984.
ReactomeiR-SCE-174403. Glutathione synthesis and recycling.

Names & Taxonomyi

Protein namesi
Recommended name:
5-oxoprolinase (EC:3.5.2.9)
Alternative name(s):
5-oxo-L-prolinase
Short name:
5-OPase
Pyroglutamase
Gene namesi
Name:OXP1
Ordered Locus Names:YKL215C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL215C.
SGDiS000001698. OXP1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi11 – 111D → A: Impairs ATPase and 5-oxoprolinase activity. 1 Publication
Mutagenesisi324 – 3241D → A: Impairs ATPase and 5-oxoprolinase activity. 1 Publication
Mutagenesisi497 – 4971G → A: Impairs ATPase and 5-oxoprolinase activity; when associated with A-498. 1 Publication
Mutagenesisi498 – 4981G → A: Impairs ATPase and 5-oxoprolinase activity; when associated with A-497. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 128612865-oxoprolinasePRO_0000208582Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei930 – 9301PhosphoserineCombined sources
Modified residuei1077 – 10771PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP28273.

PTM databases

iPTMnetiP28273.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi33950. 16 interactions.
DIPiDIP-6558N.
IntActiP28273. 2 interactions.
MINTiMINT-692651.

Structurei

3D structure databases

ProteinModelPortaliP28273.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the oxoprolinase family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000013463.
InParanoidiP28273.
KOiK01469.
OMAiMSTCISA.
OrthoDBiEOG092C067X.

Family and domain databases

InterProiIPR008040. Hydant_A_N.
IPR002821. Hydantoinase_A.
IPR003692. Hydantoinase_B.
[Graphical view]
PfamiPF05378. Hydant_A_N. 1 hit.
PF01968. Hydantoinase_A. 1 hit.
PF02538. Hydantoinase_B. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P28273-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQKGNIRIAI DKGGTFTDCV GNIGTGKQEH DTVIKLLSVD PKNYPDAPLE
60 70 80 90 100
GIRRLLEVLE HKTIPRGIPL DISNVRSLRM GTTLATNCAL ERNGERCAFI
110 120 130 140 150
TTKGFKDSLL IGDQTRPDIF NLNIKKVVPL YDTVVEIDER VTLEDFSEDP
160 170 180 190 200
YFTKSSPNEQ EGILEGNSGE MVRVIKKPDE SSVRSILKVL YASGIKSIAI
210 220 230 240 250
AFLHSYTFPD HERIVGNIAR EIGFSHVSLS SEVSPMIKFL PRAHSSVADA
260 270 280 290 300
YLTPVIKKYL NSISAGLSHA EDTHIQFMQS DGGLVDGGKF SGLKSILSGP
310 320 330 340 350
AGGVIGYSST CYDKNNNIPL IGFDMGGTST DVSRYGDGRL EHVFETVTAG
360 370 380 390 400
IIIQSPQLDI HTVAAGGSSI LSWKNGLFRV GPDSAAADPG PAAYRKGGPL
410 420 430 440 450
TITDANLFLG RLVPEFFPKI FGPNEDESLD LETTTLKFRE LTDVINKDLN
460 470 480 490 500
SNLTMEEVAY GFIKVANECM ARPVRAITEA KGHVVSQHRL VSFGGAGGQH
510 520 530 540 550
AIAVADSLGI DTVLIHRYSS ILSAYGIFLA DVIEENQEPC SFILGEPETI
560 570 580 590 600
LKVKKRFLEL SKNSIKNLLS QSFSREDIVL ERYLNLRYEG TETSLMILQK
610 620 630 640 650
YDDQWNFREW FSEAHKKEFG FSFDDKRIII DDIRIRAIGK SGVRKEKTVD
660 670 680 690 700
EQLIEISHFK KADVSKDASF TQKAYFDNKW VDTAVFKIDD LPAGTIIEGP
710 720 730 740 750
AILADGTQTN IILPNSQATI LNSHIFIKIN QKAAKTLSKS GYELDIDPIL
760 770 780 790 800
LSIFSHRFMD IALQMGTQLR KTSVSTNVKE RLDFSCALFD SKGNLVANAP
810 820 830 840 850
HVPVHLGSMS TCISAQAKLW EGKLKPGDVL ITNHPDIGGT HLPDITVITP
860 870 880 890 900
SFSSTGELIF YVASRAHHAD IGGILPGSVP PNSKELYEEG TAIYSELVVK
910 920 930 940 950
EGIFQEELIY KLFVEDPGKY PGCSGSRRFS DNISDLKAQV AANTKGIQLI
960 970 980 990 1000
GSLTKEYDLA TILKYMAAIQ TNASESIKKM LAKMVEHFGT TKFSGEDRLD
1010 1020 1030 1040 1050
DGSLIKLQVI IRPEKEEYIF NFDGTSPQVY GNLNAPEAIT NSAILYCLRC
1060 1070 1080 1090 1100
LVGEDIPLNQ GCLKPLTIKI PAGSLLSPRS GAAVVGGNVL TSQRVTDVIL
1110 1120 1130 1140 1150
KTFNVMADSQ GDCNNFTFGT GGNSGNKTDK QIKGFGYYET ICGGSGAGAD
1160 1170 1180 1190 1200
SWRGSGWNGS DAVHTNMTNT RMTDTEVFER RYPVLLKEFS IRRGSGGKGK
1210 1220 1230 1240 1250
YTGGNGVVRD VQFRKAVTAS ILSERRVIGP HGIKGGQDGS RGENLWVRHS
1260 1270 1280
TGALINVGGK NTIYAQPGDR FIIKTPGGGG FGQYKD
Length:1,286
Mass (Da):140,428
Last modified:June 1, 1994 - v2
Checksum:i9A0B8C609B5D6FFF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75951 Genomic DNA. Translation: CAA53558.1.
Z28215 Genomic DNA. Translation: CAA82060.1.
X59371 Genomic DNA. Translation: CAA42015.1.
M83295 Genomic DNA. Translation: AAA34567.1.
BK006944 Genomic DNA. Translation: DAA08954.1.
PIRiS38058.
RefSeqiNP_012707.1. NM_001179780.1.

Genome annotation databases

EnsemblFungiiYKL215C; YKL215C; YKL215C.
GeneIDi853665.
KEGGisce:YKL215C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75951 Genomic DNA. Translation: CAA53558.1.
Z28215 Genomic DNA. Translation: CAA82060.1.
X59371 Genomic DNA. Translation: CAA42015.1.
M83295 Genomic DNA. Translation: AAA34567.1.
BK006944 Genomic DNA. Translation: DAA08954.1.
PIRiS38058.
RefSeqiNP_012707.1. NM_001179780.1.

3D structure databases

ProteinModelPortaliP28273.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33950. 16 interactions.
DIPiDIP-6558N.
IntActiP28273. 2 interactions.
MINTiMINT-692651.

PTM databases

iPTMnetiP28273.

Proteomic databases

MaxQBiP28273.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL215C; YKL215C; YKL215C.
GeneIDi853665.
KEGGisce:YKL215C.

Organism-specific databases

EuPathDBiFungiDB:YKL215C.
SGDiS000001698. OXP1.

Phylogenomic databases

GeneTreeiENSGT00390000013463.
InParanoidiP28273.
KOiK01469.
OMAiMSTCISA.
OrthoDBiEOG092C067X.

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-6163-MONOMER.
YEAST:G3O-31973-MONOMER.
BRENDAi3.5.2.9. 984.
ReactomeiR-SCE-174403. Glutathione synthesis and recycling.

Miscellaneous databases

PROiP28273.

Family and domain databases

InterProiIPR008040. Hydant_A_N.
IPR002821. Hydantoinase_A.
IPR003692. Hydantoinase_B.
[Graphical view]
PfamiPF05378. Hydant_A_N. 1 hit.
PF01968. Hydantoinase_A. 1 hit.
PF02538. Hydantoinase_B. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOPLA_YEAST
AccessioniPrimary (citable) accession number: P28273
Secondary accession number(s): D6VWY8, O60212
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: June 1, 1994
Last modified: September 7, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3180 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.