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P28273

- OPLA_YEAST

UniProt

P28273 - OPLA_YEAST

Protein

5-oxoprolinase

Gene

OXP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 2 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Catalyzes the cleavage of 5-oxo-L-proline to form L-glutamate coupled to the hydrolysis of ATP to ADP and inorganic phosphate.2 Publications

    Catalytic activityi

    ATP + 5-oxo-L-proline + 2 H2O = ADP + phosphate + L-glutamate.

    Kineticsi

    1. KM=159 µM for 5-oxoproline1 Publication

    Vmax=3.5 nmol/h/µg enzyme1 Publication

    GO - Molecular functioni

    1. 5-oxoprolinase (ATP-hydrolyzing) activity Source: SGD
    2. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. glutathione metabolic process Source: SGD

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciRETL1328306-WGS:GSTH-6163-MONOMER.
    YEAST:G3O-31973-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    5-oxoprolinase (EC:3.5.2.9)
    Alternative name(s):
    5-oxo-L-prolinase
    Short name:
    5-OPase
    Pyroglutamase
    Gene namesi
    Name:OXP1
    Ordered Locus Names:YKL215C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XI

    Organism-specific databases

    CYGDiYKL215c.
    SGDiS000001698. OXP1.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi11 – 111D → A: Impairs ATPase and 5-oxoprolinase activity. 1 Publication
    Mutagenesisi324 – 3241D → A: Impairs ATPase and 5-oxoprolinase activity. 1 Publication
    Mutagenesisi497 – 4971G → A: Impairs ATPase and 5-oxoprolinase activity; when associated with A-498. 1 Publication
    Mutagenesisi498 – 4981G → A: Impairs ATPase and 5-oxoprolinase activity; when associated with A-497. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 128612865-oxoprolinasePRO_0000208582Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei930 – 9301Phosphoserine1 Publication
    Modified residuei1077 – 10771Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP28273.
    PaxDbiP28273.
    PeptideAtlasiP28273.

    Expressioni

    Gene expression databases

    GenevestigatoriP28273.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    HSP104P315391EBI-27022,EBI-8050
    SSA1P105911EBI-27022,EBI-8591

    Protein-protein interaction databases

    BioGridi33950. 14 interactions.
    DIPiDIP-6558N.
    IntActiP28273. 2 interactions.
    MINTiMINT-692651.

    Structurei

    3D structure databases

    ProteinModelPortaliP28273.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the oxoprolinase family.Curated

    Phylogenomic databases

    eggNOGiCOG0146.
    GeneTreeiENSGT00390000013463.
    KOiK01469.
    OMAiYEGTETS.
    OrthoDBiEOG7966RF.

    Family and domain databases

    InterProiIPR008040. Hydant_A_N.
    IPR002821. Hydantoinase_A.
    IPR003692. Hydantoinase_B.
    [Graphical view]
    PfamiPF05378. Hydant_A_N. 1 hit.
    PF01968. Hydantoinase_A. 1 hit.
    PF02538. Hydantoinase_B. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P28273-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQKGNIRIAI DKGGTFTDCV GNIGTGKQEH DTVIKLLSVD PKNYPDAPLE     50
    GIRRLLEVLE HKTIPRGIPL DISNVRSLRM GTTLATNCAL ERNGERCAFI 100
    TTKGFKDSLL IGDQTRPDIF NLNIKKVVPL YDTVVEIDER VTLEDFSEDP 150
    YFTKSSPNEQ EGILEGNSGE MVRVIKKPDE SSVRSILKVL YASGIKSIAI 200
    AFLHSYTFPD HERIVGNIAR EIGFSHVSLS SEVSPMIKFL PRAHSSVADA 250
    YLTPVIKKYL NSISAGLSHA EDTHIQFMQS DGGLVDGGKF SGLKSILSGP 300
    AGGVIGYSST CYDKNNNIPL IGFDMGGTST DVSRYGDGRL EHVFETVTAG 350
    IIIQSPQLDI HTVAAGGSSI LSWKNGLFRV GPDSAAADPG PAAYRKGGPL 400
    TITDANLFLG RLVPEFFPKI FGPNEDESLD LETTTLKFRE LTDVINKDLN 450
    SNLTMEEVAY GFIKVANECM ARPVRAITEA KGHVVSQHRL VSFGGAGGQH 500
    AIAVADSLGI DTVLIHRYSS ILSAYGIFLA DVIEENQEPC SFILGEPETI 550
    LKVKKRFLEL SKNSIKNLLS QSFSREDIVL ERYLNLRYEG TETSLMILQK 600
    YDDQWNFREW FSEAHKKEFG FSFDDKRIII DDIRIRAIGK SGVRKEKTVD 650
    EQLIEISHFK KADVSKDASF TQKAYFDNKW VDTAVFKIDD LPAGTIIEGP 700
    AILADGTQTN IILPNSQATI LNSHIFIKIN QKAAKTLSKS GYELDIDPIL 750
    LSIFSHRFMD IALQMGTQLR KTSVSTNVKE RLDFSCALFD SKGNLVANAP 800
    HVPVHLGSMS TCISAQAKLW EGKLKPGDVL ITNHPDIGGT HLPDITVITP 850
    SFSSTGELIF YVASRAHHAD IGGILPGSVP PNSKELYEEG TAIYSELVVK 900
    EGIFQEELIY KLFVEDPGKY PGCSGSRRFS DNISDLKAQV AANTKGIQLI 950
    GSLTKEYDLA TILKYMAAIQ TNASESIKKM LAKMVEHFGT TKFSGEDRLD 1000
    DGSLIKLQVI IRPEKEEYIF NFDGTSPQVY GNLNAPEAIT NSAILYCLRC 1050
    LVGEDIPLNQ GCLKPLTIKI PAGSLLSPRS GAAVVGGNVL TSQRVTDVIL 1100
    KTFNVMADSQ GDCNNFTFGT GGNSGNKTDK QIKGFGYYET ICGGSGAGAD 1150
    SWRGSGWNGS DAVHTNMTNT RMTDTEVFER RYPVLLKEFS IRRGSGGKGK 1200
    YTGGNGVVRD VQFRKAVTAS ILSERRVIGP HGIKGGQDGS RGENLWVRHS 1250
    TGALINVGGK NTIYAQPGDR FIIKTPGGGG FGQYKD 1286
    Length:1,286
    Mass (Da):140,428
    Last modified:June 1, 1994 - v2
    Checksum:i9A0B8C609B5D6FFF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X75951 Genomic DNA. Translation: CAA53558.1.
    Z28215 Genomic DNA. Translation: CAA82060.1.
    X59371 Genomic DNA. Translation: CAA42015.1.
    M83295 Genomic DNA. Translation: AAA34567.1.
    BK006944 Genomic DNA. Translation: DAA08954.1.
    PIRiS38058.
    RefSeqiNP_012707.1. NM_001179780.1.

    Genome annotation databases

    EnsemblFungiiYKL215C; YKL215C; YKL215C.
    GeneIDi853665.
    KEGGisce:YKL215C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X75951 Genomic DNA. Translation: CAA53558.1 .
    Z28215 Genomic DNA. Translation: CAA82060.1 .
    X59371 Genomic DNA. Translation: CAA42015.1 .
    M83295 Genomic DNA. Translation: AAA34567.1 .
    BK006944 Genomic DNA. Translation: DAA08954.1 .
    PIRi S38058.
    RefSeqi NP_012707.1. NM_001179780.1.

    3D structure databases

    ProteinModelPortali P28273.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33950. 14 interactions.
    DIPi DIP-6558N.
    IntActi P28273. 2 interactions.
    MINTi MINT-692651.

    Proteomic databases

    MaxQBi P28273.
    PaxDbi P28273.
    PeptideAtlasi P28273.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YKL215C ; YKL215C ; YKL215C .
    GeneIDi 853665.
    KEGGi sce:YKL215C.

    Organism-specific databases

    CYGDi YKL215c.
    SGDi S000001698. OXP1.

    Phylogenomic databases

    eggNOGi COG0146.
    GeneTreei ENSGT00390000013463.
    KOi K01469.
    OMAi YEGTETS.
    OrthoDBi EOG7966RF.

    Enzyme and pathway databases

    BioCyci RETL1328306-WGS:GSTH-6163-MONOMER.
    YEAST:G3O-31973-MONOMER.

    Miscellaneous databases

    NextBioi 974599.
    PROi P28273.

    Gene expression databases

    Genevestigatori P28273.

    Family and domain databases

    InterProi IPR008040. Hydant_A_N.
    IPR002821. Hydantoinase_A.
    IPR003692. Hydantoinase_B.
    [Graphical view ]
    Pfami PF05378. Hydant_A_N. 1 hit.
    PF01968. Hydantoinase_A. 1 hit.
    PF02538. Hydantoinase_B. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete sequencing of a 24.6 kb segment of yeast chromosome XI identified the known loci URA1, SAC1 and TRP3, and revealed 6 new open reading frames including homologues to the threonine dehydratases, membrane transporters, hydantoinases and the phospholipase A2-activating protein."
      Tzermia M., Horaitis O., Alexandraki D.
      Yeast 10:663-679(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "Complete DNA sequence of yeast chromosome XI."
      Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
      , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
      Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Nucleotide sequence of the URA1 gene of Saccharomyces cerevisiae."
      Roy A.
      Gene 118:149-150(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1003-1285.
      Strain: ATCC 28383 / FL100 / VTT C-80102.
    5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1077, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-930, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Metabolomic analysis via reversed-phase ion-pairing liquid chromatography coupled to a stand alone orbitrap mass spectrometer."
      Lu W., Clasquin M.F., Melamud E., Amador-Noguez D., Caudy A.A., Rabinowitz J.D.
      Anal. Chem. 82:3212-3221(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    10. "OXP1/YKL215c encodes an ATP-dependent 5-oxoprolinase in Saccharomyces cerevisiae: functional characterization, domain structure and identification of actin-like ATP-binding motifs in eukaryotic 5-oxoprolinases."
      Kumar A., Bachhawat A.K.
      FEMS Yeast Res. 10:394-401(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF ASP-11; ASP-324; GLY-497 AND GLY-498.
    11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiOPLA_YEAST
    AccessioniPrimary (citable) accession number: P28273
    Secondary accession number(s): D6VWY8, O60212
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 107 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 3180 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XI
      Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

    External Data

    Dasty 3