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Protein

Cytoplasmic aconitate hydratase

Gene

Aco1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Iron sensor. Binds a 4Fe-4S cluster and functions as aconitase when cellular iron levels are high. Functions as mRNA binding protein that regulates uptake, sequestration and utilization of iron when cellular iron levels are low. Binds to iron-responsive elements (IRES) in target mRNA species when iron levels are low. Binding of a 4Fe-4S cluster precludes RNA binding (By similarity).By similarity
Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.By similarity

Catalytic activityi

Citrate = isocitrate.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei86SubstrateBy similarity1
Metal bindingi437Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi503Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi506Iron-sulfur (4Fe-4S)By similarity1
Binding sitei536SubstrateBy similarity1
Binding sitei541SubstrateBy similarity1
Binding sitei699SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cellular iron ion homeostasis Source: MGI
  • citrate metabolic process Source: UniProtKB
  • intestinal absorption Source: MGI
  • post-embryonic development Source: MGI
  • regulation of gene expression Source: MGI
  • regulation of translation Source: MGI
  • response to iron(II) ion Source: UniProtKB
  • tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, RNA-binding

Enzyme and pathway databases

BRENDAi4.2.1.3. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytoplasmic aconitate hydratase (EC:4.2.1.3)
Short name:
Aconitase
Alternative name(s):
Citrate hydro-lyase
Iron regulatory protein 1
Short name:
IRP1
Iron-responsive element-binding protein 1
Short name:
IRE-BP 1
Gene namesi
Name:Aco1
Synonyms:Ireb1, Irebp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:87879. Aco1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytosol Source: MGI
  • endoplasmic reticulum Source: MGI
  • extracellular exosome Source: MGI
  • Golgi apparatus Source: MGI
  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000766811 – 889Cytoplasmic aconitate hydrataseAdd BLAST889

Proteomic databases

EPDiP28271.
MaxQBiP28271.
PaxDbiP28271.
PeptideAtlasiP28271.
PRIDEiP28271.

2D gel databases

REPRODUCTION-2DPAGEP28271.
SWISS-2DPAGEP28271.

PTM databases

iPTMnetiP28271.
PhosphoSitePlusiP28271.

Expressioni

Gene expression databases

BgeeiENSMUSG00000028405.
CleanExiMM_ACO1.
GenevisibleiP28271. MM.

Interactioni

Subunit structurei

Interacts (when associated with the 4Fe-4S) with FBXL5.By similarity

Protein-protein interaction databases

BioGridi197924. 1 interactor.
STRINGi10090.ENSMUSP00000100038.

Structurei

3D structure databases

ProteinModelPortaliP28271.
SMRiP28271.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni205 – 207Substrate bindingBy similarity3
Regioni779 – 780Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the aconitase/IPM isomerase family.Curated

Phylogenomic databases

eggNOGiKOG0452. Eukaryota.
COG1048. LUCA.
GeneTreeiENSGT00860000133751.
HOGENOMiHOG000025704.
HOVERGENiHBG052147.
InParanoidiP28271.
KOiK01681.
OMAiENLAKWG.
OrthoDBiEOG091G01IX.
TreeFamiTF313476.

Family and domain databases

Gene3Di3.20.19.10. 1 hit.
3.30.499.10. 3 hits.
3.40.1060.10. 1 hit.
InterProiIPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015928. Aconitase/3IPM_dehydase_swvl.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR006249. Aconitase/IRP2.
IPR018136. Aconitase_4Fe-4S_BS.
IPR000573. AconitaseA/IPMdHydase_ssu_swvl.
IPR029784. IRE-BP1.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 4 hits.
PTHR11670:SF32. PTHR11670:SF32. 4 hits.
PfamiPF00330. Aconitase. 1 hit.
PF00694. Aconitase_C. 1 hit.
[Graphical view]
PRINTSiPR00415. ACONITASE.
SUPFAMiSSF52016. SSF52016. 1 hit.
SSF53732. SSF53732. 1 hit.
TIGRFAMsiTIGR01341. aconitase_1. 1 hit.
PROSITEiPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P28271-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNPFAHLAE PLDAAQPGKR FFNLNKLEDS RYGRLPFSIR VLLEAAVRNC
60 70 80 90 100
DEFLVKKNDI ENILNWNVMQ HKNIEVPFKP ARVILQDFTG VPAVVDFAAM
110 120 130 140 150
RDAVKKLGGN PEKINPVCPA DLVIDHSIQV DFNRRADSLQ KNQDLEFERN
160 170 180 190 200
KERFEFLKWG SQAFCNMRII PPGSGIIHQV NLEYLARVVF DQDGCYYPDS
210 220 230 240 250
LVGTDSHTTM IDGLGVLGWG VGGIEAEAVM LGQPISMVLP QVIGYKLMGK
260 270 280 290 300
PHPLVTSTDI VLTITKHLRQ VGVVGKFVEF FGPGVAQLSI ADRATIANMC
310 320 330 340 350
PEYGATAAFF PVDEVSIAYL LQTGREEDKV KHIQKYLQAV GMFRDFNDTS
360 370 380 390 400
QDPDFTQVVE LDLKTVVPCC SGPKRPQDKV AVSEMKKDFE SCLGAKQGFK
410 420 430 440 450
GFQVAPDRHN DRKTFLYSNS EFTLAHGSVV IAAITSCTNT SNPSVMLGAG
460 470 480 490 500
LLAKKAVEAG LSVKPYIKTS LSPGSGVVTY YLRESGVMPY LSQLGFDVVG
510 520 530 540 550
YGCMTCIGNS GPLPEPVVEA ITQGDLVAVG VLSGNRNFEG RVHPNTRANY
560 570 580 590 600
LASPPLVIAY AIAGTVRIDF EKEPLGVNAQ GRQVFLKDIW PTRDEIQAVE
610 620 630 640 650
RQHVIPGMFK EVYQKIETVN KSWNALAAPS EKLYAWNPKS TYIKSPPFFE
660 670 680 690 700
SLTLDLQPPK SIVDAYVLLN LGDSVTTDHI SPAGNIARNS PAARYLTNRG
710 720 730 740 750
LTPREFNSYG SRRGNDAIMA RGTFANIRLL NKFLNKQAPQ TVHLPSGETL
760 770 780 790 800
DVFDAAERYQ QAGLPLIVLA GKEYGSGSSR DWAAKGPFLL GIKAVLAESY
810 820 830 840 850
ERIHRSNLVG MGVIPLEYLP GETADSLGLT GRERYTINIP EDLKPRMTVQ
860 870 880
IKLDTGKTFQ AVMRFDTDVE LTYFHNGGIL NYMIRKMAQ
Length:889
Mass (Da):98,126
Last modified:July 27, 2011 - v3
Checksum:i704B8A018EAC6B4C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti406P → S in AAH05454 (PubMed:15489334).Curated1
Sequence conflicti418S → N in AAH05454 (PubMed:15489334).Curated1
Sequence conflicti436S → T in CAA43455 (PubMed:1956798).Curated1
Sequence conflicti603H → Y in AAH05454 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61147 mRNA. Translation: CAA43455.1.
AK163985 mRNA. Translation: BAE37570.1.
AL831793 Genomic DNA. Translation: CAM27573.1.
CH466538 Genomic DNA. Translation: EDL05446.1.
BC005454 mRNA. Translation: AAH05454.1.
CCDSiCCDS18042.1.
PIRiS18720.
RefSeqiNP_031412.2. NM_007386.2.
UniGeneiMm.331547.

Genome annotation databases

EnsembliENSMUST00000102973; ENSMUSP00000100038; ENSMUSG00000028405.
GeneIDi11428.
KEGGimmu:11428.
UCSCiuc008shd.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61147 mRNA. Translation: CAA43455.1.
AK163985 mRNA. Translation: BAE37570.1.
AL831793 Genomic DNA. Translation: CAM27573.1.
CH466538 Genomic DNA. Translation: EDL05446.1.
BC005454 mRNA. Translation: AAH05454.1.
CCDSiCCDS18042.1.
PIRiS18720.
RefSeqiNP_031412.2. NM_007386.2.
UniGeneiMm.331547.

3D structure databases

ProteinModelPortaliP28271.
SMRiP28271.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi197924. 1 interactor.
STRINGi10090.ENSMUSP00000100038.

PTM databases

iPTMnetiP28271.
PhosphoSitePlusiP28271.

2D gel databases

REPRODUCTION-2DPAGEP28271.
SWISS-2DPAGEP28271.

Proteomic databases

EPDiP28271.
MaxQBiP28271.
PaxDbiP28271.
PeptideAtlasiP28271.
PRIDEiP28271.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102973; ENSMUSP00000100038; ENSMUSG00000028405.
GeneIDi11428.
KEGGimmu:11428.
UCSCiuc008shd.1. mouse.

Organism-specific databases

CTDi48.
MGIiMGI:87879. Aco1.

Phylogenomic databases

eggNOGiKOG0452. Eukaryota.
COG1048. LUCA.
GeneTreeiENSGT00860000133751.
HOGENOMiHOG000025704.
HOVERGENiHBG052147.
InParanoidiP28271.
KOiK01681.
OMAiENLAKWG.
OrthoDBiEOG091G01IX.
TreeFamiTF313476.

Enzyme and pathway databases

BRENDAi4.2.1.3. 3474.

Miscellaneous databases

ChiTaRSiAco1. mouse.
PROiP28271.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028405.
CleanExiMM_ACO1.
GenevisibleiP28271. MM.

Family and domain databases

Gene3Di3.20.19.10. 1 hit.
3.30.499.10. 3 hits.
3.40.1060.10. 1 hit.
InterProiIPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015928. Aconitase/3IPM_dehydase_swvl.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR006249. Aconitase/IRP2.
IPR018136. Aconitase_4Fe-4S_BS.
IPR000573. AconitaseA/IPMdHydase_ssu_swvl.
IPR029784. IRE-BP1.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 4 hits.
PTHR11670:SF32. PTHR11670:SF32. 4 hits.
PfamiPF00330. Aconitase. 1 hit.
PF00694. Aconitase_C. 1 hit.
[Graphical view]
PRINTSiPR00415. ACONITASE.
SUPFAMiSSF52016. SSF52016. 1 hit.
SSF53732. SSF53732. 1 hit.
TIGRFAMsiTIGR01341. aconitase_1. 1 hit.
PROSITEiPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACOC_MOUSE
AccessioniPrimary (citable) accession number: P28271
Secondary accession number(s): Q3TQ15, Q99K54
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 155 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.