Reviewed, UniProtKB/Swiss-Prot P28271 (ACOC_MOUSE)

Names and origin

Protein names

Recommended name:
    Cytoplasmic aconitate hydratase
      Short name=Aconitase
    EC=4.2.1.3
Alternative name(s):
    Citrate hydro-lyase
    Iron-responsive element-binding protein 1
      Short name=IRE-BP 1
    Iron regulatory protein 1
      Short name=IRP1

Gene names

Name:	Aco1
Synonyms:	Ireb1, Irebp

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	889 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA. This protein also expresses aconitase activity.
Catalytic activity	Citrate = isocitrate.
Cofactor	Binds 1 4Fe-4S cluster per subunit By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the aconitase/IPM isomerase family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding RNA-binding
Molecular function	Lyase
Gene Ontology (GO)
Biological process	cellular iron ion homeostasis Inferred from genetic interaction. Source: MGI intestinal absorption Inferred from genetic interaction. Source: MGI post-embryonic development Inferred from genetic interaction. Source: MGI regulation of translation Inferred from mutant phenotype. Source: MGI tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from direct assay. Source: MGI
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW aconitate hydratase activity Inferred from direct assay. Source: MGI iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW iron-responsive element binding Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 889	889	Cytoplasmic aconitate hydratase		PRO_0000076681
Sites
	Metal binding	437	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	503	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	506	1	Iron-sulfur (4Fe-4S) By similarity
Experimental info
	Sequence conflict	406	1	P → S in AAH05454. Ref.2
	Sequence conflict	418	1	N → S in CAA43455. Ref.1
	Sequence conflict	436	1	S → T in CAA43455. Ref.1
	Sequence conflict	603	1	Y → H in CAA43455. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P28271-1 [UniParc]. Last modified April 26, 2004. Version 2. Checksum: 04F0B3968B1FA7BE	FASTA	889	98,179
	10 20 30 40 50 60 MKNPFAHLAE PLDAAQPGKR FFNLNKLEDS RYGRLPFSIR VLLEAAVRNC DEFLVKKNDI 70 80 90 100 110 120 ENILNWNVMQ HKNIEVPFKP ARVILQDFTG VPAVVDFAAM RDAVKKLGGN PEKINPVCPA 130 140 150 160 170 180 DLVIDHSIQV DFNRRADSLQ KNQDLEFERN KERFEFLKWG SQAFCNMRII PPGSGIIHQV 190 200 210 220 230 240 NLEYLARVVF DQDGCYYPDS LVGTDSHTTM IDGLGVLGWG VGGIEAEAVM LGQPISMVLP 250 260 270 280 290 300 QVIGYKLMGK PHPLVTSTDI VLTITKHLRQ VGVVGKFVEF FGPGVAQLSI ADRATIANMC 310 320 330 340 350 360 PEYGATAAFF PVDEVSIAYL LQTGREEDKV KHIQKYLQAV GMFRDFNDTS QDPDFTQVVE 370 380 390 400 410 420 LDLKTVVPCC SGPKRPQDKV AVSEMKKDFE SCLGAKQGFK GFQVAPDRHN DRKTFLYNNS 430 440 450 460 470 480 EFTLAHGSVV IAAITSCTNT SNPSVMLGAG LLAKKAVEAG LSVKPYIKTS LSPGSGVVTY 490 500 510 520 530 540 YLRESGVMPY LSQLGFDVVG YGCMTCIGNS GPLPEPVVEA ITQGDLVAVG VLSGNRNFEG 550 560 570 580 590 600 RVHPNTRANY LASPPLVIAY AIAGTVRIDF EKEPLGVNAQ GRQVFLKDIW PTRDEIQAVE 610 620 630 640 650 660 RQYVIPGMFK EVYQKIETVN KSWNALAAPS EKLYAWNPKS TYIKSPPFFE SLTLDLQPPK 670 680 690 700 710 720 SIVDAYVLLN LGDSVTTDHI SPAGNIARNS PAARYLTNRG LTPREFNSYG SRRGNDAIMA 730 740 750 760 770 780 RGTFANIRLL NKFLNKQAPQ TVHLPSGETL DVFDAAERYQ QAGLPLIVLA GKEYGSGSSR 790 800 810 820 830 840 DWAAKGPFLL GIKAVLAESY ERIHRSNLVG MGVIPLEYLP GETADSLGLT GRERYTINIP 850 860 870 880 EDLKPRMTVQ IKLDTGKTFQ AVMRFDTDVE LTYFHNGGIL NYMIRKMAQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and expression of the murine iron-responsive element binding protein." Philpott C.C., Rouault T.A., Klausner R.D. Nucleic Acids Res. 19:6333-6333(1991) [PubMed: 1956798] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X61147 mRNA. Translation: CAA43455.1. BC005454 mRNA. Translation: AAH05454.1.
IPI	IPI00622780.
PIR	S18720.
UniGene	Mm.331547
3D structure databases
SMR	P28271. Positions 3-889.
ModBase	Search...
PTM databases
PhosphoSite	P28271.
2-D gel databases
SWISS-2DPAGE	P28271.
REPRODUCTION-2DPAGE	P28271.
Proteomic databases
PRIDE	P28271.
Genome annotation databases
Ensembl	ENSMUSG00000028405. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:87879. Aco1.
Phylogenomic databases
HOVERGEN	P28271.
Enzyme and pathway databases
BRENDA	4.2.1.3. 244.
Gene expression databases
ArrayExpress	P28271.
Bgee	P28271.
CleanEx	MM_ACO1.
GermOnline	ENSMUSG00000028405. Mus musculus.
Family and domain databases
InterPro	IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3. IPR001030. Acoase/IPM_deHydtase_lsu_aba. IPR015937. Aconitase-like_core. IPR015928. Aconitase/3IPM_dehydase_swvl. IPR006249. Aconitase/Fe_reg_prot_2. IPR015934. Aconitase/Fe_reg_prot_2/AcnD. IPR015932. Aconitase/IPMdHydase_lsu_aba_2. IPR018136. Aconitase_4Fe-4S_BS. IPR000573. AconitaseA/IPMdHydase_ssu_swvl. [Graphical view]
Gene3D	G3DSA:3.30.499.10. Acnase/IPM_dHydase_lsu_aba_1/3. 2 hits. G3DSA:3.20.19.10. Aconitase/3IPM_dehydase_swvl. 1 hit. G3DSA:3.40.1060.10. Aconitase/IPMdHydase_lsu_aba_2. 1 hit.
PANTHER	PTHR11670. Aconitase-like_core. 1 hit. PTHR11670:SF1. Aconitase/Fe_reg_prot_2/AcnD. 1 hit.
Pfam	PF00330. Aconitase. 1 hit. PF00694. Aconitase_C. 1 hit. [Graphical view]
PRINTS	PR00415. ACONITASE.
ProDom	PD000511. Aconitase_N. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01341. aconitase_1. 1 hit.
PROSITE	PS00450. ACONITASE_1. 1 hit. PS01244. ACONITASE_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

ACOC_MOUSE

Accession

Primary (citable) accession number: P28271
Secondary accession number(s): Q99K54

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	April 26, 2004
Last modified:	June 16, 2009
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)