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Protein

Ubiquitin-conjugating enzyme E2-24 kDa

Gene

UBC8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins. Capable, in vitro, to ubiquitinate histone H2A. Has a role in the negative regulation of gluconeogenesis. Required for proteasome-dependent catabolite degradation of fructose-1,6-bisphosphatase (FBPase).2 PublicationsPROSITE-ProRule annotation

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei85 – 851Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. acid-amino acid ligase activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. ubiquitin-protein transferase activity Source: SGD

GO - Biological processi

  1. negative regulation of gluconeogenesis Source: SGD
  2. proteasome-mediated ubiquitin-dependent protein catabolic process Source: SGD
  3. protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30138-MONOMER.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2-24 kDa (EC:6.3.2.19)
Alternative name(s):
Glucose-induced degradation protein 3
Ubiquitin carrier protein
Ubiquitin-protein ligase
Gene namesi
Name:UBC8
Synonyms:GID3
Ordered Locus Names:YEL012W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

CYGDiYEL012w.
SGDiS000000738. UBC8.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 218218Ubiquitin-conjugating enzyme E2-24 kDaPRO_0000082555Add
BLAST

Proteomic databases

MaxQBiP28263.
PaxDbiP28263.
PeptideAtlasiP28263.

Expressioni

Gene expression databases

GenevestigatoriP28263.

Interactioni

Protein-protein interaction databases

BioGridi36719. 85 interactions.
DIPiDIP-5299N.
IntActiP28263. 1 interaction.
MINTiMINT-473957.
STRINGi4932.YEL012W.

Structurei

3D structure databases

ProteinModelPortaliP28263.
SMRiP28263. Positions 2-150.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5078.
GeneTreeiENSGT00390000004852.
HOGENOMiHOG000233452.
InParanoidiP28263.
KOiK10576.
OMAiYQLENIF.
OrthoDBiEOG7SBP18.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P28263-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSSKRRIET DVMKLLMSDH QVDLINDSMQ EFHVKFLGPK DTPYENGVWR
60 70 80 90 100
LHVELPDNYP YKSPSIGFVN KIFHPNIDIA SGSICLDVIN STWSPLYDLI
110 120 130 140 150
NIVEWMIPGL LKEPNGSDPL NNEAATLQLR DKKLYEEKIK EYIDKYATKE
160 170 180 190 200
KYQQMFGGDN DSDDSDSGGD LQEEDSDSDE DMDGTGVSSG DDSVDELSED
210
LSDIDVSDDD DYDEVANQ
Length:218
Mass (Da):24,633
Last modified:June 1, 1994 - v2
Checksum:i24FFAD8B4679CE6F
GO

Sequence cautioni

The sequence AAB64489.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M65083 Genomic DNA. No translation available.
U18530 Genomic DNA. Translation: AAB64489.1. Different initiation.
Z29329 Genomic DNA. Translation: CAA82526.1.
BK006939 Genomic DNA. Translation: DAA07641.1.
PIRiB53516.
RefSeqiNP_010904.2. NM_001178827.1.

Genome annotation databases

EnsemblFungiiYEL012W; YEL012W; YEL012W.
GeneIDi856704.
KEGGisce:YEL012W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M65083 Genomic DNA. No translation available.
U18530 Genomic DNA. Translation: AAB64489.1. Different initiation.
Z29329 Genomic DNA. Translation: CAA82526.1.
BK006939 Genomic DNA. Translation: DAA07641.1.
PIRiB53516.
RefSeqiNP_010904.2. NM_001178827.1.

3D structure databases

ProteinModelPortaliP28263.
SMRiP28263. Positions 2-150.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36719. 85 interactions.
DIPiDIP-5299N.
IntActiP28263. 1 interaction.
MINTiMINT-473957.
STRINGi4932.YEL012W.

Proteomic databases

MaxQBiP28263.
PaxDbiP28263.
PeptideAtlasiP28263.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYEL012W; YEL012W; YEL012W.
GeneIDi856704.
KEGGisce:YEL012W.

Organism-specific databases

CYGDiYEL012w.
SGDiS000000738. UBC8.

Phylogenomic databases

eggNOGiCOG5078.
GeneTreeiENSGT00390000004852.
HOGENOMiHOG000233452.
InParanoidiP28263.
KOiK10576.
OMAiYQLENIF.
OrthoDBiEOG7SBP18.

Enzyme and pathway databases

UniPathwayiUPA00143.
BioCyciYEAST:G3O-30138-MONOMER.

Miscellaneous databases

NextBioi982771.
PROiP28263.

Gene expression databases

GenevestigatoriP28263.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of a Saccharomyces cerevisiae gene encoding a new member of the ubiquitin-conjugating protein family."
    Qin S., Nakajima B., Nomura M., Arfin S.M.
    J. Biol. Chem. 266:15549-15554(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "A human ubiquitin-conjugating enzyme homologous to yeast UBC8."
    Kaiser P., Seufert W., Hoefferer L., Kofler B., Sachsenmaier C., Herzog H., Jentsch S., Schweiger M., Schneider R.
    J. Biol. Chem. 269:8797-8802(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION TO N-TERMINUS.
  5. "Ubc8p functions in catabolite degradation of fructose-1, 6-bisphosphatase in yeast."
    Schuele T., Rose M., Entian K.-D., Thumm M., Wolf D.H.
    EMBO J. 19:2161-2167(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  6. "Catabolite degradation of fructose-1,6-bisphosphatase in the yeast Saccharomyces cerevisiae: a genome-wide screen identifies eight novel GID genes and indicates the existence of two degradation pathways."
    Regelmann J., Schuele T., Josupeit F.S., Horak J., Rose M., Entian K.-D., Thumm M., Wolf D.H.
    Mol. Biol. Cell 14:1652-1663(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiUBC8_YEAST
AccessioniPrimary (citable) accession number: P28263
Secondary accession number(s): D3DLN7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: June 1, 1994
Last modified: January 7, 2015
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1590 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.