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P28263 (UBC8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin-conjugating enzyme E2-24 kDa

EC=6.3.2.19
Alternative name(s):
Glucose-induced degradation protein 3
Ubiquitin carrier protein
Ubiquitin-protein ligase
Gene names
Name:UBC8
Synonyms:GID3
Ordered Locus Names:YEL012W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length218 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the covalent attachment of ubiquitin to other proteins. Capable, in vitro, to ubiquitinate histone H2A. Has a role in the negative regulation of gluconeogenesis. Required for proteasome-dependent catabolite degradation of fructose-1,6-bisphosphatase (FBPase). Ref.5 Ref.6

Catalytic activity

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathway

Protein modification; protein ubiquitination.

Subcellular location

Cytoplasm Ref.5.

Miscellaneous

Present with 1590 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family.

Sequence caution

The sequence AAB64489.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 218218Ubiquitin-conjugating enzyme E2-24 kDa
PRO_0000082555

Sites

Active site851Glycyl thioester intermediate By similarity

Sequences

Sequence LengthMass (Da)Tools
P28263 [UniParc].

Last modified June 1, 1994. Version 2.
Checksum: 24FFAD8B4679CE6F

FASTA21824,633
        10         20         30         40         50         60 
MSSSKRRIET DVMKLLMSDH QVDLINDSMQ EFHVKFLGPK DTPYENGVWR LHVELPDNYP 

        70         80         90        100        110        120 
YKSPSIGFVN KIFHPNIDIA SGSICLDVIN STWSPLYDLI NIVEWMIPGL LKEPNGSDPL 

       130        140        150        160        170        180 
NNEAATLQLR DKKLYEEKIK EYIDKYATKE KYQQMFGGDN DSDDSDSGGD LQEEDSDSDE 

       190        200        210 
DMDGTGVSSG DDSVDELSED LSDIDVSDDD DYDEVANQ 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of a Saccharomyces cerevisiae gene encoding a new member of the ubiquitin-conjugating protein family."
Qin S., Nakajima B., Nomura M., Arfin S.M.
J. Biol. Chem. 266:15549-15554(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome V."
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. expand/collapse author list , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.
Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"A human ubiquitin-conjugating enzyme homologous to yeast UBC8."
Kaiser P., Seufert W., Hoefferer L., Kofler B., Sachsenmaier C., Herzog H., Jentsch S., Schweiger M., Schneider R.
J. Biol. Chem. 269:8797-8802(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION TO N-TERMINUS.
[5]"Ubc8p functions in catabolite degradation of fructose-1, 6-bisphosphatase in yeast."
Schuele T., Rose M., Entian K.-D., Thumm M., Wolf D.H.
EMBO J. 19:2161-2167(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]"Catabolite degradation of fructose-1,6-bisphosphatase in the yeast Saccharomyces cerevisiae: a genome-wide screen identifies eight novel GID genes and indicates the existence of two degradation pathways."
Regelmann J., Schuele T., Josupeit F.S., Horak J., Rose M., Entian K.-D., Thumm M., Wolf D.H.
Mol. Biol. Cell 14:1652-1663(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M65083 Genomic DNA. No translation available.
U18530 Genomic DNA. Translation: AAB64489.1. Different initiation.
Z29329 Genomic DNA. Translation: CAA82526.1.
BK006939 Genomic DNA. Translation: DAA07641.1.
PIRB53516.
RefSeqNP_010904.2. NM_001178827.1.

3D structure databases

ProteinModelPortalP28263.
SMRP28263. Positions 2-150.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36719. 84 interactions.
DIPDIP-5299N.
IntActP28263. 1 interaction.
MINTMINT-473957.
STRING4932.YEL012W.

Proteomic databases

PaxDbP28263.
PeptideAtlasP28263.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYEL012W; YEL012W; YEL012W.
GeneID856704.
KEGGsce:YEL012W.

Organism-specific databases

CYGDYEL012w.
SGDS000000738. UBC8.

Phylogenomic databases

eggNOGCOG5078.
GeneTreeENSGT00390000004852.
HOGENOMHOG000233452.
KOK10576.
OMAVINSTWS.
OrthoDBEOG7SBP18.

Enzyme and pathway databases

BioCycYEAST:G3O-30138-MONOMER.
UniPathwayUPA00143.

Gene expression databases

GenevestigatorP28263.

Family and domain databases

Gene3D3.10.110.10. 1 hit.
InterProIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMSSF54495. SSF54495. 1 hit.
PROSITEPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio982771.
PROP28263.

Entry information

Entry nameUBC8_YEAST
AccessionPrimary (citable) accession number: P28263
Secondary accession number(s): D3DLN7
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: June 1, 1994
Last modified: February 19, 2014
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways