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P28263

- UBC8_YEAST

UniProt

P28263 - UBC8_YEAST

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Protein
Ubiquitin-conjugating enzyme E2-24 kDa
Gene
UBC8, GID3, YEL012W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins. Capable, in vitro, to ubiquitinate histone H2A. Has a role in the negative regulation of gluconeogenesis. Required for proteasome-dependent catabolite degradation of fructose-1,6-bisphosphatase (FBPase).2 Publications

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei85 – 851Glycyl thioester intermediate By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. acid-amino acid ligase activity Source: InterPro
  3. ubiquitin-protein transferase activity Source: SGD

GO - Biological processi

  1. negative regulation of gluconeogenesis Source: SGD
  2. proteasome-mediated ubiquitin-dependent protein catabolic process Source: SGD
  3. protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30138-MONOMER.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2-24 kDa (EC:6.3.2.19)
Alternative name(s):
Glucose-induced degradation protein 3
Ubiquitin carrier protein
Ubiquitin-protein ligase
Gene namesi
Name:UBC8
Synonyms:GID3
Ordered Locus Names:YEL012W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

CYGDiYEL012w.
SGDiS000000738. UBC8.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 218218Ubiquitin-conjugating enzyme E2-24 kDa
PRO_0000082555Add
BLAST

Proteomic databases

MaxQBiP28263.
PaxDbiP28263.
PeptideAtlasiP28263.

Expressioni

Gene expression databases

GenevestigatoriP28263.

Interactioni

Protein-protein interaction databases

BioGridi36719. 84 interactions.
DIPiDIP-5299N.
IntActiP28263. 1 interaction.
MINTiMINT-473957.
STRINGi4932.YEL012W.

Structurei

3D structure databases

ProteinModelPortaliP28263.
SMRiP28263. Positions 2-150.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG5078.
GeneTreeiENSGT00390000004852.
HOGENOMiHOG000233452.
KOiK10576.
OMAiIWIERER.
OrthoDBiEOG7SBP18.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P28263-1 [UniParc]FASTAAdd to Basket

« Hide

MSSSKRRIET DVMKLLMSDH QVDLINDSMQ EFHVKFLGPK DTPYENGVWR    50
LHVELPDNYP YKSPSIGFVN KIFHPNIDIA SGSICLDVIN STWSPLYDLI 100
NIVEWMIPGL LKEPNGSDPL NNEAATLQLR DKKLYEEKIK EYIDKYATKE 150
KYQQMFGGDN DSDDSDSGGD LQEEDSDSDE DMDGTGVSSG DDSVDELSED 200
LSDIDVSDDD DYDEVANQ 218
Length:218
Mass (Da):24,633
Last modified:June 1, 1994 - v2
Checksum:i24FFAD8B4679CE6F
GO

Sequence cautioni

The sequence AAB64489.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M65083 Genomic DNA. No translation available.
U18530 Genomic DNA. Translation: AAB64489.1. Different initiation.
Z29329 Genomic DNA. Translation: CAA82526.1.
BK006939 Genomic DNA. Translation: DAA07641.1.
PIRiB53516.
RefSeqiNP_010904.2. NM_001178827.1.

Genome annotation databases

EnsemblFungiiYEL012W; YEL012W; YEL012W.
GeneIDi856704.
KEGGisce:YEL012W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M65083 Genomic DNA. No translation available.
U18530 Genomic DNA. Translation: AAB64489.1 . Different initiation.
Z29329 Genomic DNA. Translation: CAA82526.1 .
BK006939 Genomic DNA. Translation: DAA07641.1 .
PIRi B53516.
RefSeqi NP_010904.2. NM_001178827.1.

3D structure databases

ProteinModelPortali P28263.
SMRi P28263. Positions 2-150.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36719. 84 interactions.
DIPi DIP-5299N.
IntActi P28263. 1 interaction.
MINTi MINT-473957.
STRINGi 4932.YEL012W.

Proteomic databases

MaxQBi P28263.
PaxDbi P28263.
PeptideAtlasi P28263.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YEL012W ; YEL012W ; YEL012W .
GeneIDi 856704.
KEGGi sce:YEL012W.

Organism-specific databases

CYGDi YEL012w.
SGDi S000000738. UBC8.

Phylogenomic databases

eggNOGi COG5078.
GeneTreei ENSGT00390000004852.
HOGENOMi HOG000233452.
KOi K10576.
OMAi IWIERER.
OrthoDBi EOG7SBP18.

Enzyme and pathway databases

UniPathwayi UPA00143 .
BioCyci YEAST:G3O-30138-MONOMER.

Miscellaneous databases

NextBioi 982771.
PROi P28263.

Gene expression databases

Genevestigatori P28263.

Family and domain databases

Gene3Di 3.10.110.10. 1 hit.
InterProi IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view ]
Pfami PF00179. UQ_con. 1 hit.
[Graphical view ]
SUPFAMi SSF54495. SSF54495. 1 hit.
PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of a Saccharomyces cerevisiae gene encoding a new member of the ubiquitin-conjugating protein family."
    Qin S., Nakajima B., Nomura M., Arfin S.M.
    J. Biol. Chem. 266:15549-15554(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "A human ubiquitin-conjugating enzyme homologous to yeast UBC8."
    Kaiser P., Seufert W., Hoefferer L., Kofler B., Sachsenmaier C., Herzog H., Jentsch S., Schweiger M., Schneider R.
    J. Biol. Chem. 269:8797-8802(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION TO N-TERMINUS.
  5. "Ubc8p functions in catabolite degradation of fructose-1, 6-bisphosphatase in yeast."
    Schuele T., Rose M., Entian K.-D., Thumm M., Wolf D.H.
    EMBO J. 19:2161-2167(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  6. "Catabolite degradation of fructose-1,6-bisphosphatase in the yeast Saccharomyces cerevisiae: a genome-wide screen identifies eight novel GID genes and indicates the existence of two degradation pathways."
    Regelmann J., Schuele T., Josupeit F.S., Horak J., Rose M., Entian K.-D., Thumm M., Wolf D.H.
    Mol. Biol. Cell 14:1652-1663(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiUBC8_YEAST
AccessioniPrimary (citable) accession number: P28263
Secondary accession number(s): D3DLN7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: June 1, 1994
Last modified: September 3, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1590 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3

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