Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P28263

- UBC8_YEAST

UniProt

P28263 - UBC8_YEAST

Protein

Ubiquitin-conjugating enzyme E2-24 kDa

Gene

UBC8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 2 (01 Jun 1994)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the covalent attachment of ubiquitin to other proteins. Capable, in vitro, to ubiquitinate histone H2A. Has a role in the negative regulation of gluconeogenesis. Required for proteasome-dependent catabolite degradation of fructose-1,6-bisphosphatase (FBPase).2 PublicationsPROSITE-ProRule annotation

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei85 – 851Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. ubiquitin-protein transferase activity Source: SGD

    GO - Biological processi

    1. negative regulation of gluconeogenesis Source: SGD
    2. proteasome-mediated ubiquitin-dependent protein catabolic process Source: SGD
    3. protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: SGD

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30138-MONOMER.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-conjugating enzyme E2-24 kDa (EC:6.3.2.19)
    Alternative name(s):
    Glucose-induced degradation protein 3
    Ubiquitin carrier protein
    Ubiquitin-protein ligase
    Gene namesi
    Name:UBC8
    Synonyms:GID3
    Ordered Locus Names:YEL012W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome V

    Organism-specific databases

    CYGDiYEL012w.
    SGDiS000000738. UBC8.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 218218Ubiquitin-conjugating enzyme E2-24 kDaPRO_0000082555Add
    BLAST

    Proteomic databases

    MaxQBiP28263.
    PaxDbiP28263.
    PeptideAtlasiP28263.

    Expressioni

    Gene expression databases

    GenevestigatoriP28263.

    Interactioni

    Protein-protein interaction databases

    BioGridi36719. 85 interactions.
    DIPiDIP-5299N.
    IntActiP28263. 1 interaction.
    MINTiMINT-473957.
    STRINGi4932.YEL012W.

    Structurei

    3D structure databases

    ProteinModelPortaliP28263.
    SMRiP28263. Positions 2-150.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5078.
    GeneTreeiENSGT00390000004852.
    HOGENOMiHOG000233452.
    KOiK10576.
    OMAiIWIERER.
    OrthoDBiEOG7SBP18.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 1 hit.
    PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P28263-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSSKRRIET DVMKLLMSDH QVDLINDSMQ EFHVKFLGPK DTPYENGVWR    50
    LHVELPDNYP YKSPSIGFVN KIFHPNIDIA SGSICLDVIN STWSPLYDLI 100
    NIVEWMIPGL LKEPNGSDPL NNEAATLQLR DKKLYEEKIK EYIDKYATKE 150
    KYQQMFGGDN DSDDSDSGGD LQEEDSDSDE DMDGTGVSSG DDSVDELSED 200
    LSDIDVSDDD DYDEVANQ 218
    Length:218
    Mass (Da):24,633
    Last modified:June 1, 1994 - v2
    Checksum:i24FFAD8B4679CE6F
    GO

    Sequence cautioni

    The sequence AAB64489.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M65083 Genomic DNA. No translation available.
    U18530 Genomic DNA. Translation: AAB64489.1. Different initiation.
    Z29329 Genomic DNA. Translation: CAA82526.1.
    BK006939 Genomic DNA. Translation: DAA07641.1.
    PIRiB53516.
    RefSeqiNP_010904.2. NM_001178827.1.

    Genome annotation databases

    EnsemblFungiiYEL012W; YEL012W; YEL012W.
    GeneIDi856704.
    KEGGisce:YEL012W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M65083 Genomic DNA. No translation available.
    U18530 Genomic DNA. Translation: AAB64489.1 . Different initiation.
    Z29329 Genomic DNA. Translation: CAA82526.1 .
    BK006939 Genomic DNA. Translation: DAA07641.1 .
    PIRi B53516.
    RefSeqi NP_010904.2. NM_001178827.1.

    3D structure databases

    ProteinModelPortali P28263.
    SMRi P28263. Positions 2-150.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36719. 85 interactions.
    DIPi DIP-5299N.
    IntActi P28263. 1 interaction.
    MINTi MINT-473957.
    STRINGi 4932.YEL012W.

    Proteomic databases

    MaxQBi P28263.
    PaxDbi P28263.
    PeptideAtlasi P28263.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YEL012W ; YEL012W ; YEL012W .
    GeneIDi 856704.
    KEGGi sce:YEL012W.

    Organism-specific databases

    CYGDi YEL012w.
    SGDi S000000738. UBC8.

    Phylogenomic databases

    eggNOGi COG5078.
    GeneTreei ENSGT00390000004852.
    HOGENOMi HOG000233452.
    KOi K10576.
    OMAi IWIERER.
    OrthoDBi EOG7SBP18.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    BioCyci YEAST:G3O-30138-MONOMER.

    Miscellaneous databases

    NextBioi 982771.
    PROi P28263.

    Gene expression databases

    Genevestigatori P28263.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 1 hit.
    PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of a Saccharomyces cerevisiae gene encoding a new member of the ubiquitin-conjugating protein family."
      Qin S., Nakajima B., Nomura M., Arfin S.M.
      J. Biol. Chem. 266:15549-15554(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "A human ubiquitin-conjugating enzyme homologous to yeast UBC8."
      Kaiser P., Seufert W., Hoefferer L., Kofler B., Sachsenmaier C., Herzog H., Jentsch S., Schweiger M., Schneider R.
      J. Biol. Chem. 269:8797-8802(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: SEQUENCE REVISION TO N-TERMINUS.
    5. "Ubc8p functions in catabolite degradation of fructose-1, 6-bisphosphatase in yeast."
      Schuele T., Rose M., Entian K.-D., Thumm M., Wolf D.H.
      EMBO J. 19:2161-2167(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    6. "Catabolite degradation of fructose-1,6-bisphosphatase in the yeast Saccharomyces cerevisiae: a genome-wide screen identifies eight novel GID genes and indicates the existence of two degradation pathways."
      Regelmann J., Schuele T., Josupeit F.S., Horak J., Rose M., Entian K.-D., Thumm M., Wolf D.H.
      Mol. Biol. Cell 14:1652-1663(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiUBC8_YEAST
    AccessioniPrimary (citable) accession number: P28263
    Secondary accession number(s): D3DLN7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 130 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1590 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome V
      Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

    External Data

    Dasty 3