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Protein

Ubiquitin-conjugating enzyme E2-24 kDa

Gene

UBC8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins. Capable, in vitro, to ubiquitinate histone H2A. Has a role in the negative regulation of gluconeogenesis. Required for proteasome-dependent catabolite degradation of fructose-1,6-bisphosphatase (FBPase).PROSITE-ProRule annotation2 Publications

Miscellaneous

Present with 1590 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei85Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • negative regulation of gluconeogenesis Source: SGD
  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: SGD
  • ubiquitin-dependent protein catabolic process Source: SGD

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30138-MONOMER
ReactomeiR-SCE-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes
R-SCE-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2-24 kDa (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme 8
Glucose-induced degradation protein 3
Ubiquitin carrier protein
Ubiquitin-protein ligase
Gene namesi
Name:UBC8
Synonyms:GID3
Ordered Locus Names:YEL012W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YEL012W
SGDiS000000738 UBC8

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000825551 – 218Ubiquitin-conjugating enzyme E2-24 kDaAdd BLAST218

Proteomic databases

MaxQBiP28263
PaxDbiP28263
PRIDEiP28263

PTM databases

CarbonylDBiP28263

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
VIP1Q066853EBI-19755,EBI-35034

GO - Molecular functioni

Protein-protein interaction databases

BioGridi36719, 104 interactors
DIPiDIP-5299N
IntActiP28263, 9 interactors
MINTiP28263
STRINGi4932.YEL012W

Structurei

3D structure databases

ProteinModelPortaliP28263
SMRiP28263
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00390000004852
HOGENOMiHOG000233452
InParanoidiP28263
KOiK10576
OMAiNLVNDNM
OrthoDBiEOG092C50OL

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequencei

Sequence statusi: Complete.

P28263-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSKRRIET DVMKLLMSDH QVDLINDSMQ EFHVKFLGPK DTPYENGVWR
60 70 80 90 100
LHVELPDNYP YKSPSIGFVN KIFHPNIDIA SGSICLDVIN STWSPLYDLI
110 120 130 140 150
NIVEWMIPGL LKEPNGSDPL NNEAATLQLR DKKLYEEKIK EYIDKYATKE
160 170 180 190 200
KYQQMFGGDN DSDDSDSGGD LQEEDSDSDE DMDGTGVSSG DDSVDELSED
210
LSDIDVSDDD DYDEVANQ
Length:218
Mass (Da):24,633
Last modified:June 1, 1994 - v2
Checksum:i24FFAD8B4679CE6F
GO

Sequence cautioni

The sequence AAB64489 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M65083 Genomic DNA No translation available.
U18530 Genomic DNA Translation: AAB64489.1 Different initiation.
Z29329 Genomic DNA Translation: CAA82526.1
BK006939 Genomic DNA Translation: DAA07641.1
PIRiB53516
RefSeqiNP_010904.2, NM_001178827.1

Genome annotation databases

EnsemblFungiiYEL012W; YEL012W; YEL012W
GeneIDi856704
KEGGisce:YEL012W

Similar proteinsi

Entry informationi

Entry nameiUBC8_YEAST
AccessioniPrimary (citable) accession number: P28263
Secondary accession number(s): D3DLN7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: June 1, 1994
Last modified: April 25, 2018
This is version 158 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health