P28263 (UBC8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-conjugating enzyme E2-24 kDa
EC=6.3.2.19

Alternative name(s):
Glucose-induced degradation protein 3
Ubiquitin carrier protein
Ubiquitin-protein ligase

Gene names

Name:	UBC8
Synonyms:	GID3
Ordered Locus Names:	YEL012W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	218 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins. Capable, in vitro, to ubiquitinate histone H2A. Has a role in the negative regulation of gluconeogenesis. Required for proteasome-dependent catabolite degradation of fructose-1,6-bisphosphatase (FBPase). Ref.5 Ref.6
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Subcellular location	Cytoplasm Ref.5.
Miscellaneous	Present with 1590 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.
Sequence caution	The sequence AAB64489.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	negative regulation of gluconeogenesis Inferred from mutant phenotype Ref.6. Source: SGD proteasomal ubiquitin-dependent protein catabolic process Inferred from mutant phenotype Ref.6. Source: SGD protein monoubiquitination Traceable author statement. Source: SGD protein polyubiquitination Traceable author statement. Source: SGD protein ubiquitination involved in ubiquitin-dependent protein catabolic process Inferred from mutant phenotype Ref.5. Source: SGD
Cellular component	cytoplasm Inferred from direct assay Ref.5. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from direct assay Ref.1. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 218	218	Ubiquitin-conjugating enzyme E2-24 kDa		PRO_0000082555
Sites
	Active site	85	1	Glycyl thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P28263 [UniParc]. Last modified June 1, 1994. Version 2. Checksum: 24FFAD8B4679CE6F	FASTA	218	24,633
	10 20 30 40 50 60 MSSSKRRIET DVMKLLMSDH QVDLINDSMQ EFHVKFLGPK DTPYENGVWR LHVELPDNYP 70 80 90 100 110 120 YKSPSIGFVN KIFHPNIDIA SGSICLDVIN STWSPLYDLI NIVEWMIPGL LKEPNGSDPL 130 140 150 160 170 180 NNEAATLQLR DKKLYEEKIK EYIDKYATKE KYQQMFGGDN DSDDSDSGGD LQEEDSDSDE 190 200 210 DMDGTGVSSG DDSVDELSED LSDIDVSDDD DYDEVANQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of a Saccharomyces cerevisiae gene encoding a new member of the ubiquitin-conjugating protein family." Qin S., Nakajima B., Nomura M., Arfin S.M. J. Biol. Chem. 266:15549-15554(1991) [PubMed: 1869573] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"A human ubiquitin-conjugating enzyme homologous to yeast UBC8." Kaiser P., Seufert W., Hoefferer L., Kofler B., Sachsenmaier C., Herzog H., Jentsch S., Schweiger M., Schneider R. J. Biol. Chem. 269:8797-8802(1994) [PubMed: 8132613] [Abstract] Cited for: SEQUENCE REVISION TO N-TERMINUS.
[5]	"Ubc8p functions in catabolite degradation of fructose-1, 6-bisphosphatase in yeast." Schuele T., Rose M., Entian K.-D., Thumm M., Wolf D.H. EMBO J. 19:2161-2167(2000) [PubMed: 10811607] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]	"Catabolite degradation of fructose-1,6-bisphosphatase in the yeast Saccharomyces cerevisiae: a genome-wide screen identifies eight novel GID genes and indicates the existence of two degradation pathways." Regelmann J., Schuele T., Josupeit F.S., Horak J., Rose M., Entian K.-D., Thumm M., Wolf D.H. Mol. Biol. Cell 14:1652-1663(2003) [PubMed: 12686616] [Abstract] Cited for: FUNCTION.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M65083 Genomic DNA. No translation available. U18530 Genomic DNA. Translation: AAB64489.1. Different initiation. Z29329 Genomic DNA. Translation: CAA82526.1. BK006939 Genomic DNA. Translation: DAA07641.1.
PIR	B53516.
RefSeq	NP_010904.2. NM_001178827.1.
3D structure databases
ProteinModelPortal	P28263.
SMR	P28263. Positions 2-150.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5299N.
IntAct	P28263. 4 interactions.
MINT	MINT-473957.
STRING	P28263.
Proteomic databases
PeptideAtlas	P28263.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YEL012W; YEL012W; YEL012W.
GeneID	856704.
KEGG	sce:YEL012W.
NMPDR	fig|4932.3.peg.1960.
Organism-specific databases
CYGD	YEL012w.
SGD	S000000738. UBC8.
Phylogenomic databases
eggNOG	fuNOG05597.
GeneTree	EFGT00050000001509.
HOGENOM	HBG756483.
OMA	VINSTWS.
OrthoDB	EOG46HKM1.
Gene expression databases
ArrayExpress	P28263.
Genevestigator	P28263.
GermOnline	YEL012W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000608. UBQ-conjugat_E2. IPR023313. UBQ-conjugating_AS. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Gene3D	G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.
KO	K10576.
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	982771.

Entry information

Entry name

UBC8_YEAST

Accession

Primary (citable) accession number: P28263
Secondary accession number(s): D3DLN7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	June 1, 1994
Last modified:	December 14, 2011
This is version 109 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program