Deoxycytidine triphosphate deaminase - Escherichia coli (strain K12)

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Reviewed, UniProtKB/Swiss-Prot P28248 (DCD_ECOLI)

Last modified June 16, 2009. Version 72. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Deoxycytidine triphosphate deaminase
      Short name=dCTP deaminase
    EC=3.5.4.13

Gene names

Name:	dcd
Synonyms:	dus, paxA
Ordered Locus Names:	b2065, JW2050

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length	193 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Catalytic activity	dCTP + H₂O = dUTP + NH₃. HAMAP MF_00146
Pathway	Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 1/2. HAMAP MF_00146
Subunit structure	Homotetramer Probable.
Sequence similarities	Belongs to the dCTP deaminase family.

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Ontologies

Keywords
Biological process	Nucleotide metabolism
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	dUTP biosynthetic process Inferred from electronic annotation. Source: InterPro pyrimidine ribonucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	dCTP deaminase activity Inferred from electronic annotation. Source: HAMAP protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
groS	P0A6F9	1	EBI-1122141,EBI-369169

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 193

193

Deoxycytidine triphosphate deaminase HAMAP MF_00146

PRO_0000155984

Secondary structure

193

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P28248-1 [UniParc].

Last modified December 1, 1992. Version 1.
Checksum: B0044051ADE7F919
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FASTA

193

21,249

        10         20         30         40         50         60 
MRLCDRDIEA WLDEGRLSIN PRPPVERING ATVDVRLGNK FRTFRGHTAA FIDLSGPKDE 

        70         80         90        100        110        120 
VSAALDRVMS DEIVLDEGEA FYLHPGELAL AVTLESVTLP ADLVGWLDGR SSLARLGLMV 

       130        140        150        160        170        180 
HVTAHRIDPG WSGCIVLEFY NSGKLPLALR PGMLIGALSF EPLSGPAVRP YNRREDAKYR 

       190 
NQQGAVASRI DKD

« Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"dcd (dCTP deaminase) gene of Escherichia coli: mapping, cloning, sequencing, and identification as a locus of suppressors of lethal dut (dUTPase) mutations." Wang L., Weiss B. J. Bacteriol. 174:5647-5653(1992) [PubMed: 1324907] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10. Strain: K12.
[2]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. Horiuchi T. DNA Res. 3:379-392(1996) [PubMed: 9097040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

M90069 Genomic DNA. Translation: AAA23669.1.
U00096 Genomic DNA. Translation: AAC75126.1.
AP009048 Genomic DNA. Translation: BAA15918.1.

PIR

A42940.

RefSeq

AP_002665.1.
NP_416569.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1XS1	X-ray	1.80	A/B/C/D/E/F	1-193	[»]
1XS4	X-ray	2.53	A/B/C/D/E/F	1-193	[»]
1XS6	X-ray	2.00	A/B/C/D/E/F	1-193	[»]
2J4H	X-ray	2.70	A/B	1-193	[»]
2J4Q	X-ray	2.60	A/B	1-193	[»]
2V9X	X-ray	2.20	A/B/C/D/E/F/G/H/I/J/K/L	1-193	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P28248. 2 interactions.

Genome annotation databases

GeneID

946593.

GenomeReviews

Gene locus JW2050 in contig AP009048_GR.
Gene locus b2065 in contig U00096_GR.

KEGG

ecj:JW2050.
eco:b2065.

Organism-specific databases

EchoBASE

EB1389.

EcoGene

EG11418. dcd.

CMR

Search...

Phylogenomic databases

HOGENOM

P28248.

OMA

P28248. GSRYQGQ.

Enzyme and pathway databases

BioCyc

EcoCyc:DCTP-DEAM-MON.
MetaCyc:DCTP-DEAM-MON.

Family and domain databases

HAMAP

MF_00146.
[Tree]

InterPro

IPR011962. dCTP_deam.
IPR008180. DeoxyUTPase.
[Graphical view]

Pfam

PF00692. dUTPase. 1 hit.
[Graphical view]

ProDom

PD004900. dCTP_deaminase. 1 hit.
[Graphical view] [Entries sharing at least one domain]

TIGRFAMs

TIGR02274. dCTP_deam. 1 hit.

ProtoNet

Search...

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Entry information

Entry name

DCD_ECOLI

Accession

Primary (citable) accession number: P28248

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	June 16, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents