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Protein

dCTP deaminase

Gene

dcd

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

dCTP + H2O = dUTP + NH3.UniRule annotation1 Publication

Pathwayi: dUMP biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes dUMP from dCTP (dUTP route).UniRule annotation1 Publication
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. dCTP deaminase (dcd)
  2. Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut)
This subpathway is part of the pathway dUMP biosynthesis, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dUMP from dCTP (dUTP route), the pathway dUMP biosynthesis and in Pyrimidine metabolism.

GO - Molecular functioni

  • dCTP deaminase activity Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Nucleotide metabolism

Enzyme and pathway databases

BioCyciEcoCyc:DCTP-DEAM-MONOMER.
ECOL316407:JW2050-MONOMER.
MetaCyc:DCTP-DEAM-MONOMER.
BRENDAi3.5.4.13. 2026.
UniPathwayiUPA00610; UER00665.

Names & Taxonomyi

Protein namesi
Recommended name:
dCTP deaminase1 PublicationUniRule annotation (EC:3.5.4.13UniRule annotation1 Publication)
Alternative name(s):
Deoxycytidine triphosphate deaminaseUniRule annotation
Gene namesi
Name:dcd1 PublicationUniRule annotation
Synonyms:dus1 Publication, paxA
Ordered Locus Names:b2065, JW2050
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11418. dcd.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Mutant accumulates dCTP.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001559841 – 193dCTP deaminaseAdd BLAST193

Proteomic databases

PaxDbiP28248.
PRIDEiP28248.

Interactioni

Subunit structurei

Homotetramer.Curated

Protein-protein interaction databases

BioGridi4259684. 4 interactors.
IntActiP28248. 2 interactors.
STRINGi511145.b2065.

Structurei

Secondary structure

1193
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 14Combined sources10
Beta strandi15 – 21Combined sources7
Helixi25 – 27Combined sources3
Beta strandi32 – 37Combined sources6
Beta strandi39 – 42Combined sources4
Helixi46 – 48Combined sources3
Beta strandi54 – 56Combined sources3
Helixi58 – 68Combined sources11
Beta strandi81 – 83Combined sources3
Beta strandi89 – 98Combined sources10
Beta strandi103 – 108Combined sources6
Helixi111 – 114Combined sources4
Turni115 – 117Combined sources3
Beta strandi118 – 120Combined sources3
Beta strandi132 – 141Combined sources10
Beta strandi143 – 145Combined sources3
Beta strandi147 – 149Combined sources3
Beta strandi153 – 162Combined sources10
Helixi171 – 173Combined sources3
Beta strandi180 – 182Combined sources3
Helixi190 – 192Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XS1X-ray1.80A/B/C/D/E/F1-193[»]
1XS4X-ray2.53A/B/C/D/E/F1-193[»]
1XS6X-ray2.00A/B/C/D/E/F1-193[»]
2J4HX-ray2.70A/B1-193[»]
2J4QX-ray2.60A/B1-193[»]
2V9XX-ray2.20A/B/C/D/E/F/G/H/I/J/K/L1-193[»]
ProteinModelPortaliP28248.
SMRiP28248.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP28248.

Family & Domainsi

Sequence similaritiesi

Belongs to the dCTP deaminase family.UniRule annotationCurated

Phylogenomic databases

eggNOGiENOG4105DHP. Bacteria.
COG0717. LUCA.
HOGENOMiHOG000228601.
InParanoidiP28248.
KOiK01494.
OMAiNIPCIHP.
PhylomeDBiP28248.

Family and domain databases

CDDicd07557. trimeric_dUTPase. 1 hit.
Gene3Di2.70.40.10. 1 hit.
HAMAPiMF_00146. dCTP_deaminase. 1 hit.
InterProiIPR011962. dCTP_deam.
IPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
IPR033704. dUTPase_trimeric.
[Graphical view]
PfamiPF00692. dUTPase. 1 hit.
[Graphical view]
SUPFAMiSSF51283. SSF51283. 1 hit.
TIGRFAMsiTIGR02274. dCTP_deam. 1 hit.

Sequencei

Sequence statusi: Complete.

P28248-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLCDRDIEA WLDEGRLSIN PRPPVERING ATVDVRLGNK FRTFRGHTAA
60 70 80 90 100
FIDLSGPKDE VSAALDRVMS DEIVLDEGEA FYLHPGELAL AVTLESVTLP
110 120 130 140 150
ADLVGWLDGR SSLARLGLMV HVTAHRIDPG WSGCIVLEFY NSGKLPLALR
160 170 180 190
PGMLIGALSF EPLSGPAVRP YNRREDAKYR NQQGAVASRI DKD
Length:193
Mass (Da):21,249
Last modified:December 1, 1992 - v1
Checksum:iB0044051ADE7F919
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M90069 Genomic DNA. Translation: AAA23669.1.
U00096 Genomic DNA. Translation: AAC75126.1.
AP009048 Genomic DNA. Translation: BAA15918.1.
PIRiA42940.
RefSeqiNP_416569.1. NC_000913.3.
WP_001234768.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75126; AAC75126; b2065.
BAA15918; BAA15918; BAA15918.
GeneIDi946593.
KEGGiecj:JW2050.
eco:b2065.
PATRICi32119463. VBIEscCol129921_2142.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M90069 Genomic DNA. Translation: AAA23669.1.
U00096 Genomic DNA. Translation: AAC75126.1.
AP009048 Genomic DNA. Translation: BAA15918.1.
PIRiA42940.
RefSeqiNP_416569.1. NC_000913.3.
WP_001234768.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XS1X-ray1.80A/B/C/D/E/F1-193[»]
1XS4X-ray2.53A/B/C/D/E/F1-193[»]
1XS6X-ray2.00A/B/C/D/E/F1-193[»]
2J4HX-ray2.70A/B1-193[»]
2J4QX-ray2.60A/B1-193[»]
2V9XX-ray2.20A/B/C/D/E/F/G/H/I/J/K/L1-193[»]
ProteinModelPortaliP28248.
SMRiP28248.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259684. 4 interactors.
IntActiP28248. 2 interactors.
STRINGi511145.b2065.

Proteomic databases

PaxDbiP28248.
PRIDEiP28248.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75126; AAC75126; b2065.
BAA15918; BAA15918; BAA15918.
GeneIDi946593.
KEGGiecj:JW2050.
eco:b2065.
PATRICi32119463. VBIEscCol129921_2142.

Organism-specific databases

EchoBASEiEB1389.
EcoGeneiEG11418. dcd.

Phylogenomic databases

eggNOGiENOG4105DHP. Bacteria.
COG0717. LUCA.
HOGENOMiHOG000228601.
InParanoidiP28248.
KOiK01494.
OMAiNIPCIHP.
PhylomeDBiP28248.

Enzyme and pathway databases

UniPathwayiUPA00610; UER00665.
BioCyciEcoCyc:DCTP-DEAM-MONOMER.
ECOL316407:JW2050-MONOMER.
MetaCyc:DCTP-DEAM-MONOMER.
BRENDAi3.5.4.13. 2026.

Miscellaneous databases

EvolutionaryTraceiP28248.
PROiP28248.

Family and domain databases

CDDicd07557. trimeric_dUTPase. 1 hit.
Gene3Di2.70.40.10. 1 hit.
HAMAPiMF_00146. dCTP_deaminase. 1 hit.
InterProiIPR011962. dCTP_deam.
IPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
IPR033704. dUTPase_trimeric.
[Graphical view]
PfamiPF00692. dUTPase. 1 hit.
[Graphical view]
SUPFAMiSSF51283. SSF51283. 1 hit.
TIGRFAMsiTIGR02274. dCTP_deam. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDCD_ECOLI
AccessioniPrimary (citable) accession number: P28248
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: November 30, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.