Deoxycytidine triphosphate deaminase - Escherichia coli (strain K12)

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P28248 (DCD_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 103. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Deoxycytidine triphosphate deaminase
Short name=dCTP deaminase
EC=3.5.4.13

Gene names

Name:	dcd
Synonyms:	dus, paxA
Ordered Locus Names:	b2065, JW2050

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	193 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	dCTP + H₂O = dUTP + NH₃. HAMAP-Rule MF_00146
Pathway	Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 1/2. HAMAP-Rule MF_00146
Subunit structure	Homotetramer Probable.
Sequence similarities	Belongs to the dCTP deaminase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	dUMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway dUTP biosynthetic process Inferred from electronic annotation. Source: InterPro nucleobase-containing small molecule interconversion Inferred from mutant phenotype PubMed 5541540. Source: EcoCyc pyrimidine ribonucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	dCTP deaminase activity Inferred from direct assay PubMed 15539408. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 193

193

Deoxycytidine triphosphate deaminase HAMAP-Rule MF_00146

PRO_0000155984

Secondary structure

193

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P28248 [UniParc].

Last modified December 1, 1992. Version 1.
Checksum: B0044051ADE7F919
Show »

FASTA

193

21,249

        10         20         30         40         50         60 
MRLCDRDIEA WLDEGRLSIN PRPPVERING ATVDVRLGNK FRTFRGHTAA FIDLSGPKDE 

        70         80         90        100        110        120 
VSAALDRVMS DEIVLDEGEA FYLHPGELAL AVTLESVTLP ADLVGWLDGR SSLARLGLMV 

       130        140        150        160        170        180 
HVTAHRIDPG WSGCIVLEFY NSGKLPLALR PGMLIGALSF EPLSGPAVRP YNRREDAKYR 

       190 
NQQGAVASRI DKD

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"dcd (dCTP deaminase) gene of Escherichia coli: mapping, cloning, sequencing, and identification as a locus of suppressors of lethal dut (dUTPase) mutations." Wang L., Weiss B. J. Bacteriol. 174:5647-5653(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10. Strain: K12.
[2]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. Horiuchi T. DNA Res. 3:379-392(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M90069 Genomic DNA. Translation: AAA23669.1.
U00096 Genomic DNA. Translation: AAC75126.1.
AP009048 Genomic DNA. Translation: BAA15918.1.

PIR

A42940.

RefSeq

NP_416569.1. NC_000913.2.
YP_490307.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1XS1	X-ray	1.80	A/B/C/D/E/F	1-193	[»]
1XS4	X-ray	2.53	A/B/C/D/E/F	1-193	[»]
1XS6	X-ray	2.00	A/B/C/D/E/F	1-193	[»]
2J4H	X-ray	2.70	A/B	1-193	[»]
2J4Q	X-ray	2.60	A/B	1-193	[»]
2V9X	X-ray	2.20	A/B/C/D/E/F/G/H/I/J/K/L	1-193	[»]

ProteinModelPortal

P28248.

SMR

P28248. Positions 1-193.

ModBase

Search...

Protein-protein interaction databases

IntAct

P28248. 2 interactions.

STRING

511145.b2065.

Proteomic databases

PaxDb

P28248.

PRIDE

P28248.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC75126; AAC75126; b2065.
BAA15918; BAA15918; BAA15918.

GeneID

12930690.
946593.

KEGG

ecj:Y75_p2028.
eco:b2065.

PATRIC

32119463. VBIEscCol129921_2142.

Organism-specific databases

EchoBASE

EB1389.

EcoGene

EG11418. dcd.

Phylogenomic databases

eggNOG

COG0717.

HOGENOM

HOG000228601.

K01494.

OMA

RQDAKYK.

ProtClustDB

PRK00416.

Enzyme and pathway databases

BioCyc

EcoCyc:DCTP-DEAM-MONOMER.
ECOL316407:JW2050-MONOMER.
MetaCyc:DCTP-DEAM-MONOMER.

UniPathway

UPA00610; UER00665.

Gene expression databases

Genevestigator

P28248.

Family and domain databases

HAMAP

MF_00146. dCTP_deaminase.

InterPro

IPR011962. dCTP_deam.
IPR008180. dUTP_pyroPase.
[Graphical view]

Pfam

PF00692. dUTPase. 1 hit.
[Graphical view]

TIGRFAMs

TIGR02274. dCTP_deam. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P28248.

Entry information

Entry name

DCD_ECOLI

Accession

Primary (citable) accession number: P28248

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	May 1, 2013
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents