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Protein

Bicyclomycin resistance protein

Gene

bcr

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance (PubMed:2694948). Probable membrane translocase. A transporter able to export peptides. When overexpressed, allows cells deleted for multiple peptidases (pepA, pepB, pepD and pepN) to grow in the presence of dipeptides Ala-Gln or Gly-Tyr which otherwise inhibit growth (PubMed:20067529). Cells overexpressing this protein have decreased intracellular levels of Ala-Gln dipeptide, and in a system that produces the Ala-Gln dipeptide overproduction of this protein increases export of the dipeptide (PubMed:20067529).1 Publication

GO - Molecular functioni

  • dipeptide transmembrane transporter activity Source: EcoCyc
  • drug transmembrane transporter activity Source: InterPro

GO - Biological processi

  • dipeptide transmembrane transport Source: EcoCyc
  • response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Antibiotic resistance, Peptide transport, Protein transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:BCR-MONOMER.
ECOL316407:JW5363-MONOMER.

Protein family/group databases

TCDBi2.A.1.2.7. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Bicyclomycin resistance protein
Alternative name(s):
Sulfonamide resistance protein
Gene namesi
Name:bcr
Synonyms:bicA, bicR, sur1 Publication, suxA
Ordered Locus Names:b2182, JW5363
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11419. bcr.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 88CytoplasmicSequence analysis
Transmembranei9 – 2618HelicalSequence analysisAdd
BLAST
Topological domaini27 – 4822PeriplasmicSequence analysisAdd
BLAST
Transmembranei49 – 6517HelicalSequence analysisAdd
BLAST
Topological domaini66 – 7611CytoplasmicSequence analysisAdd
BLAST
Transmembranei77 – 9822HelicalSequence analysisAdd
BLAST
Topological domaini99 – 1057PeriplasmicSequence analysis
Transmembranei106 – 12217HelicalSequence analysisAdd
BLAST
Topological domaini123 – 13715CytoplasmicSequence analysisAdd
BLAST
Transmembranei138 – 15922HelicalSequence analysisAdd
BLAST
Topological domaini160 – 1656PeriplasmicSequence analysis
Transmembranei166 – 18520HelicalSequence analysisAdd
BLAST
Topological domaini186 – 21429CytoplasmicSequence analysisAdd
BLAST
Transmembranei215 – 23925HelicalSequence analysisAdd
BLAST
Topological domaini240 – 25011PeriplasmicSequence analysisAdd
BLAST
Transmembranei251 – 26818HelicalSequence analysisAdd
BLAST
Topological domaini269 – 28517CytoplasmicSequence analysisAdd
BLAST
Transmembranei286 – 30217HelicalSequence analysisAdd
BLAST
Topological domaini303 – 3086PeriplasmicSequence analysis
Transmembranei309 – 33224HelicalSequence analysisAdd
BLAST
Topological domaini333 – 34412CytoplasmicSequence analysisAdd
BLAST
Transmembranei345 – 36622HelicalSequence analysisAdd
BLAST
Topological domaini367 – 3726PeriplasmicSequence analysis
Transmembranei373 – 39018HelicalSequence analysisAdd
BLAST
Topological domaini391 – 3966Cytoplasmic1 Publication

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 396396Bicyclomycin resistance proteinPRO_0000173315Add
BLAST

Proteomic databases

EPDiP28246.
PaxDbiP28246.

Interactioni

Protein-protein interaction databases

BioGridi4260471. 144 interactions.
STRINGi511145.b2182.

Structurei

3D structure databases

ProteinModelPortaliP28246.
SMRiP28246. Positions 28-362.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C2C. Bacteria.
ENOG410XNNX. LUCA.
HOGENOMiHOG000143171.
InParanoidiP28246.
KOiK07552.
OMAiITHARKG.
PhylomeDBiP28246.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR004812. Efflux_drug-R_Bcr/CmlA.
IPR011701. MFS.
IPR020846. MFS_dom.
IPR004734. Multidrug-R.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00880. 2_A_01_02. 1 hit.
TIGR00710. efflux_Bcr_CflA. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P28246-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTRQHSSFA IVFILGLLAM LMPLSIDMYL PALPVISAQF GVPAGSTQMT
60 70 80 90 100
LSTYILGFAL GQLIYGPMAD SFGRKPVVLG GTLVFAAAAV ACALANTIDQ
110 120 130 140 150
LIVMRFFHGL AAAAASVVIN ALMRDIYPKE EFSRMMSFVM LVTTIAPLMA
160 170 180 190 200
PIVGGWVLVW LSWHYIFWIL ALAAILASAM IFFLIKETLP PERRQPFHIR
210 220 230 240 250
TTIGNFAALF RHKRVLSYML ASGFSFAGMF SFLSAGPFVY IEINHVAPEN
260 270 280 290 300
FGYYFALNIV FLFVMTIFNS RFVRRIGALN MFRSGLWIQF IMAAWMVISA
310 320 330 340 350
LLGLGFWSLV VGVAAFVGCV SMVSSNAMAV ILDEFPHMAG TASSLAGTFR
360 370 380 390
FGIGAIVGAL LSLATFNSAW PMIWSIAFCA TSSILFCLYA SRPKKR
Length:396
Mass (Da):43,353
Last modified:July 19, 2003 - v4
Checksum:iD609AE35370E6A1D
GO

Sequence cautioni

The sequence AAA16406 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAA45230 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti99 – 991D → V in CAA45230 (PubMed:8486276).Curated
Sequence conflicti246 – 2461V → I (Ref. 1) Curated
Sequence conflicti246 – 2461V → I in CAA45230 (PubMed:8486276).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63703 Genomic DNA. Translation: CAA45230.1. Different initiation.
U00008 Genomic DNA. Translation: AAA16406.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75243.1.
AP009048 Genomic DNA. Translation: BAE76647.1.
PIRiE64987.
RefSeqiNP_416687.1. NC_000913.3.
WP_000213361.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75243; AAC75243; b2182.
BAE76647; BAE76647; BAE76647.
GeneIDi944808.
KEGGiecj:JW5363.
eco:b2182.
PATRICi32119719. VBIEscCol129921_2270.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63703 Genomic DNA. Translation: CAA45230.1. Different initiation.
U00008 Genomic DNA. Translation: AAA16406.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75243.1.
AP009048 Genomic DNA. Translation: BAE76647.1.
PIRiE64987.
RefSeqiNP_416687.1. NC_000913.3.
WP_000213361.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP28246.
SMRiP28246. Positions 28-362.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260471. 144 interactions.
STRINGi511145.b2182.

Protein family/group databases

TCDBi2.A.1.2.7. the major facilitator superfamily (mfs).

Proteomic databases

EPDiP28246.
PaxDbiP28246.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75243; AAC75243; b2182.
BAE76647; BAE76647; BAE76647.
GeneIDi944808.
KEGGiecj:JW5363.
eco:b2182.
PATRICi32119719. VBIEscCol129921_2270.

Organism-specific databases

EchoBASEiEB1390.
EcoGeneiEG11419. bcr.

Phylogenomic databases

eggNOGiENOG4105C2C. Bacteria.
ENOG410XNNX. LUCA.
HOGENOMiHOG000143171.
InParanoidiP28246.
KOiK07552.
OMAiITHARKG.
PhylomeDBiP28246.

Enzyme and pathway databases

BioCyciEcoCyc:BCR-MONOMER.
ECOL316407:JW5363-MONOMER.

Miscellaneous databases

PROiP28246.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR004812. Efflux_drug-R_Bcr/CmlA.
IPR011701. MFS.
IPR020846. MFS_dom.
IPR004734. Multidrug-R.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00880. 2_A_01_02. 1 hit.
TIGR00710. efflux_Bcr_CflA. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBCR_ECOLI
AccessioniPrimary (citable) accession number: P28246
Secondary accession number(s): Q2MAQ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: July 19, 2003
Last modified: September 7, 2016
This is version 132 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.