P28239 (IPYR2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inorganic pyrophosphatase, mitochondrial
EC=3.6.1.1

Alternative name(s):
Pyrophosphate phospho-hydrolase
Short name=PPase

Gene names

Name:	PPA2
Synonyms:	IPP2
Ordered Locus Names:	YMR267W
ORF Names:	YM8156.09

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in energy production. Its activity is stimulated by uncouplers of ATP synthesis.
Catalytic activity	Diphosphate + H2O = 2 phosphate.
Cofactor	Binds 4 magnesium ions per subunit. Other metal ions can support activity, but at a lower rate. Two magnesium ions are required for the activation of the enzyme and are present before substrate binds, two additional magnesium ions form complexes with substrate and product By similarity.
Subunit structure	Homodimer that binds non-covalently to a protein complex in the inner mitochondrial membrane By similarity.
Subcellular location	Mitochondrion.
Miscellaneous	Present with 195 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the PPase family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	aerobic respiration Inferred from mutant phenotype Ref.1. Source: SGD phosphate-containing compound metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrion Inferred from direct assay. Source: SGD
Molecular function	inorganic diphosphatase activity Inferred from mutant phenotype Ref.1. Source: SGD magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 30	30	Mitochondrion Potential
	Chain	31 – 310	280	Inorganic pyrophosphatase, mitochondrial		PRO_0000025413
Sites
	Metal binding	152	1	Magnesium 1 By similarity
	Metal binding	157	1	Magnesium 1 By similarity
	Metal binding	157	1	Magnesium 2 By similarity
	Metal binding	189	1	Magnesium 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P28239 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 985F6E9BDD50BA25	FASTA	310	35,573
	10 20 30 40 50 60 MNLLRMNALT SKARSIERLK QTLNILSIRN HRQFSTIQQG SKYTLGFKKY LTLLNGEVGS 70 80 90 100 110 120 FFHDVPLDLN EHEKTVNMIV EVPRWTTGKF EISKELRFNP IVQDTKNGKL RFVNNIFPYH 130 140 150 160 170 180 GYIHNYGAIP QTWEDPTIEH KLGKCDVALK GDNDPLDCCE IGSDVLEMGS IKKVKVLGSL 190 200 210 220 230 240 ALIDDGELDW KVIVIDVNDP LSSKIDDLEK IEEYFPGILD TTREWFRKYK VPAGKPLNSF 250 260 270 280 290 300 AFHEQYQNSN KTIQTIKKCH NSWKNLISGS LQEKYDNLPN TERAGNGVTL EDSVKPPSQI 310 PPEVQKWYYV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Yeast PPA2 gene encodes a mitochondrial inorganic pyrophosphatase that is essential for mitochondrial function." Lundin M., Baltscheffsky H., Ronne H. J. Biol. Chem. 266:12168-12172(1991) [PubMed: 1648084] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 208353 / W303-1A.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Computer modeling of two inorganic pyrophosphatases." Vihinen M., Lundin M., Baltscheffsky H. Biochem. Biophys. Res. Commun. 186:122-128(1992) [PubMed: 1321599] [Abstract] Cited for: 3D-STRUCTURE MODELING.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M81880 Genomic DNA. Translation: AAA34893.1. Z49260 Genomic DNA. Translation: CAA89250.1. BK006946 Genomic DNA. Translation: DAA10167.1.
PIR	A40867.
RefSeq	NP_013994.1. NM_001182774.1.
3D structure databases
ProteinModelPortal	P28239.
SMR	P28239. Positions 33-310.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1522N.
IntAct	P28239. 5 interactions.
MINT	MINT-385495.
STRING	P28239.
Proteomic databases
PeptideAtlas	P28239.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR267W; YMR267W; YMR267W.
GeneID	855309.
KEGG	sce:YMR267W.
NMPDR	fig|4932.3.peg.5050.
Organism-specific databases
CYGD	YMR267w.
SGD	S000004880. PPA2.
Phylogenomic databases
eggNOG	fuNOG04257.
GeneTree	EFGT00050000006068.
HOGENOM	HBG434750.
OMA	IMVINTE.
OrthoDB	EOG43FM63.
Gene expression databases
ArrayExpress	P28239.
Genevestigator	P28239.
GermOnline	YMR267W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR008162. Pyrophosphatase. [Graphical view]
Gene3D	G3DSA:3.90.80.10. Pyrophosphatase. 1 hit.
KO	K01507.
PANTHER	PTHR10286. Pyrophosphatase. 1 hit.
Pfam	PF00719. Pyrophosphatase. 1 hit. [Graphical view]
SUPFAM	SSF50324. Pyrophosphatase. 1 hit.
PROSITE	PS00387. PPASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	978991.

Entry information

Entry name

IPYR2_YEAST

Accession

Primary (citable) accession number: P28239
Secondary accession number(s): D6W093

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	December 14, 2011
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program