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Protein

Gap junction alpha-8 protein

Gene

Gja8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.

GO - Molecular functioni

GO - Biological processi

  • camera-type eye development Source: MGI
  • cell-cell signaling Source: Ensembl
  • lens development in camera-type eye Source: MGI
  • protein homooligomerization Source: Ensembl
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Gap junction alpha-8 protein
Alternative name(s):
Connexin-50
Short name:
Cx50
Lens fiber protein MP70
Gene namesi
Name:Gja8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:99953. Gja8.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 2322CytoplasmicSequence analysisAdd
BLAST
Transmembranei24 – 4623HelicalSequence analysisAdd
BLAST
Topological domaini47 – 7630ExtracellularSequence analysisAdd
BLAST
Transmembranei77 – 9923HelicalSequence analysisAdd
BLAST
Topological domaini100 – 15758CytoplasmicSequence analysisAdd
BLAST
Transmembranei158 – 18023HelicalSequence analysisAdd
BLAST
Topological domaini181 – 20929ExtracellularSequence analysisAdd
BLAST
Transmembranei210 – 22920HelicalSequence analysisAdd
BLAST
Topological domaini230 – 440211CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • connexon complex Source: Ensembl
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Gap junction, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 440439Gap junction alpha-8 proteinPRO_0000057831Add
BLAST

Proteomic databases

PaxDbiP28236.
PRIDEiP28236.

PTM databases

PhosphoSiteiP28236.

Expressioni

Tissue specificityi

Eye lens.

Gene expression databases

BgeeiP28236.
CleanExiMM_GJA8.
ExpressionAtlasiP28236. baseline and differential.
GenevisibleiP28236. MM.

Interactioni

Subunit structurei

A connexon is composed of a hexamer of connexins. This particular connexin only forms junctional channels.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000049532.

Structurei

3D structure databases

ProteinModelPortaliP28236.
SMRiP28236. Positions 3-234.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IF3Z. Eukaryota.
ENOG410ZC96. LUCA.
HOGENOMiHOG000231127.
HOVERGENiHBG009576.
InParanoidiP28236.
KOiK07617.
OMAiMYVGHAV.
OrthoDBiEOG7P2XSS.
PhylomeDBiP28236.
TreeFamiTF329606.

Family and domain databases

InterProiIPR000500. Connexin.
IPR002266. Connexin50.
IPR019570. Connexin_CCC.
IPR017990. Connexin_CS.
IPR013092. Connexin_N.
[Graphical view]
PANTHERiPTHR11984. PTHR11984. 2 hits.
PTHR11984:SF19. PTHR11984:SF19. 2 hits.
PfamiPF00029. Connexin. 1 hit.
PF03509. Connexin50. 1 hit.
[Graphical view]
PRINTSiPR00206. CONNEXIN.
PR01137. CONNEXINA8.
SMARTiSM00037. CNX. 1 hit.
SM01089. Connexin_CCC. 1 hit.
[Graphical view]
PROSITEiPS00407. CONNEXINS_1. 1 hit.
PS00408. CONNEXINS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28236-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDWSFLGNI LEEVNEHSTV IGRVWLTVLF IFRILILGTA AEFVWGDEQS
60 70 80 90 100
DFVCNTQQPG CENVCYDEAF PISHIRLWVL QIIFVSTPSL MYVGHAVHHV
110 120 130 140 150
RMEEKRKDRE AEELCQQSRS NGGERVPIAP DQASIRKSSS SSKGTKKFRL
160 170 180 190 200
EGTLLRTYVC HIIFKTLFEV GFIVGHYFLY GFRILPLYRC SRWPCPNVVD
210 220 230 240 250
CFVSRPTEKT IFILFMLSVA FVSLFLNIME MSHLGMKGIR SAFKRPVEQP
260 270 280 290 300
LGEIAEKSLH SIAVSSIQKA KGYQLLEEEK IVSHYFPLTE VGMVETSPLS
310 320 330 340 350
AKPFSQFEEK IGTGPLADMS RSYQETLPSY AQVGVQEVER EEPPIEEAVE
360 370 380 390 400
PEVGEKKQEA EKVAPEGQET VAVPDRERVE TPGVGKEDEK EELQAEKVTK
410 420 430 440
QGLSAEKAPS LCPELTTDDN RPLSRLSKAS SRARSDDLTI
Length:440
Mass (Da):49,598
Last modified:January 23, 2007 - v2
Checksum:i7FD80B0E15DC4C65
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M91243 Genomic DNA. Translation: AAA37497.1.
CCDSiCCDS17651.1.
PIRiI49624.
RefSeqiNP_032149.1. NM_008123.3.
UniGeneiMm.258183.
Mm.455421.

Genome annotation databases

EnsembliENSMUST00000062944; ENSMUSP00000049532; ENSMUSG00000049908.
GeneIDi14616.
KEGGimmu:14616.
UCSCiuc008qon.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M91243 Genomic DNA. Translation: AAA37497.1.
CCDSiCCDS17651.1.
PIRiI49624.
RefSeqiNP_032149.1. NM_008123.3.
UniGeneiMm.258183.
Mm.455421.

3D structure databases

ProteinModelPortaliP28236.
SMRiP28236. Positions 3-234.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000049532.

PTM databases

PhosphoSiteiP28236.

Proteomic databases

PaxDbiP28236.
PRIDEiP28236.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000062944; ENSMUSP00000049532; ENSMUSG00000049908.
GeneIDi14616.
KEGGimmu:14616.
UCSCiuc008qon.2. mouse.

Organism-specific databases

CTDi2703.
MGIiMGI:99953. Gja8.

Phylogenomic databases

eggNOGiENOG410IF3Z. Eukaryota.
ENOG410ZC96. LUCA.
HOGENOMiHOG000231127.
HOVERGENiHBG009576.
InParanoidiP28236.
KOiK07617.
OMAiMYVGHAV.
OrthoDBiEOG7P2XSS.
PhylomeDBiP28236.
TreeFamiTF329606.

Miscellaneous databases

NextBioi286428.
PROiP28236.
SOURCEiSearch...

Gene expression databases

BgeeiP28236.
CleanExiMM_GJA8.
ExpressionAtlasiP28236. baseline and differential.
GenevisibleiP28236. MM.

Family and domain databases

InterProiIPR000500. Connexin.
IPR002266. Connexin50.
IPR019570. Connexin_CCC.
IPR017990. Connexin_CS.
IPR013092. Connexin_N.
[Graphical view]
PANTHERiPTHR11984. PTHR11984. 2 hits.
PTHR11984:SF19. PTHR11984:SF19. 2 hits.
PfamiPF00029. Connexin. 1 hit.
PF03509. Connexin50. 1 hit.
[Graphical view]
PRINTSiPR00206. CONNEXIN.
PR01137. CONNEXINA8.
SMARTiSM00037. CNX. 1 hit.
SM01089. Connexin_CCC. 1 hit.
[Graphical view]
PROSITEiPS00407. CONNEXINS_1. 1 hit.
PS00408. CONNEXINS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Mouse Cx50, a functional member of the connexin family of gap junction proteins, is the lens fiber protein MP70."
    White T.W., Bruzzone R., Goodenough D.A., Paul D.L.
    Mol. Biol. Cell 3:711-720(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Lubec G., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 379-390, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: OF1.
    Tissue: Hippocampus.

Entry informationi

Entry nameiCXA8_MOUSE
AccessioniPrimary (citable) accession number: P28236
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: January 23, 2007
Last modified: May 11, 2016
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.