P28235 (CXA4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gap junction alpha-4 protein

Alternative name(s):
Connexin-37
Short name=Cx37

Gene names

Name:	Gja4
Synonyms:	Cxn-37

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	333 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
Subunit structure	A connexon is composed of a hexamer of connexins.
Subcellular location	Cell membrane; Multi-pass membrane protein. Cell junction › gap junction.
Tissue specificity	Highly expressed in lung.
Sequence similarities	Belongs to the connexin family. Alpha-type (group II) subfamily.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Gap junction Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	blood vessel development Inferred from mutant phenotype PubMed 12435353. Source: MGI calcium ion transport Inferred from electronic annotation. Source: Compara cell-cell signaling Inferred from electronic annotation. Source: Compara response to pain Inferred from electronic annotation. Source: Compara
Cellular_component	connexon complex Inferred from electronic annotation. Source: Compara integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 333	333	Gap junction alpha-4 protein		PRO_0000057815
Regions
	Topological domain	1 – 20	20	Cytoplasmic Potential
	Transmembrane	21 – 40	20	Helical; Potential
	Topological domain	41 – 76	36	Extracellular Potential
	Transmembrane	77 – 99	23	Helical; Potential
	Topological domain	100 – 148	49	Cytoplasmic Potential
	Transmembrane	149 – 171	23	Helical; Potential
	Topological domain	172 – 208	37	Extracellular Potential
	Transmembrane	209 – 231	23	Helical; Potential
	Topological domain	232 – 333	102	Cytoplasmic Potential
Natural variations
	Natural variant	54	1	C → Q Requires 2 nucleotide substitutions. Ref.3
	Natural variant	59	1	P → A. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P28235 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: C73D2B2AC0D926C1	FASTA	333	37,596
	10 20 30 40 50 60 MGDWGFLEKL LDQVQEHSTV VGKIWLTVLF IFRILILGLA GESVWGDEQS DFECNTAQPG 70 80 90 100 110 120 CTNVCYDQAF PISHIRYWVL QFLFVSTPTL IYLGHVIYLS RREERLRQKE GELRALPSKD 130 140 150 160 170 180 LHVERALAAI EHQMAKISVA EDGRLRIRGA LMGTYVVSVL CKSVLEAGFL YGQWRLYGWT 190 200 210 220 230 240 MEPVFVCQRA PCPHIVDCYV SRPTEKTIFI IFMLVVGVIS LVLNLLELVH LLCRCVSREI 250 260 270 280 290 300 KARRDHDARP AQGSASDPYP EQVFFYLPMG EGPSSPPCPT YNGLSSTEQN WANLTTEERL 310 320 330 TSSRPPPFVN TAPQGGRKSP SRPNSSASKK QYV

References

[1]	"Mouse connexin37: cloning and functional expression of a gap junction gene highly expressed in lung." Willecke K., Heynkes R., Dahl E., Stutenkemper R., Hennemann H., Jungbluth S., Suchyna T., Nicholson B.J. J. Cell Biol. 114:1049-1057(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C57BL/6.
[2]	"Mouse connexin37: gene structure and promoter analysis." Seul K.H., Beyer E.C. Biochim. Biophys. Acta 1492:499-504(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: 129/SvJ. Tissue: Lung.
[3]	"CTL induction by a tumour-associated antigen octapeptide derived from a murine lung carcinoma." Mandelboim O., Berke G., Fridkin M., Feldman M., Eisenstein M., Eisenbach L. Nature 369:67-71(1994) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 52-59, VARIANTS GLN-54 AND ALA-59. Tissue: Lung tumor.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X57971 Genomic DNA. Translation: CAA41037.1. AF216832 mRNA. Translation: AAF91221.1.
IPI	IPI00453994.
PIR	A40548.
RefSeq	NP_032146.1. NM_008120.3.
UniGene	Mm.24615.
3D structure databases
ProteinModelPortal	P28235.
SMR	P28235. Positions 3-234.
ModBase	Search...
Proteomic databases
PRIDE	P28235.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000053753; ENSMUSP00000062320; ENSMUSG00000050234.
GeneID	14612.
KEGG	mmu:14612.
Organism-specific databases
CTD	2701.
MGI	MGI:95715. Gja4.
Phylogenomic databases
eggNOG	NOG39708.
HOGENOM	HOG000231127.
HOVERGEN	HBG009576.
InParanoid	P28235.
KO	K07613.
OMA	RGALMGT.
OrthoDB	EOG4PC9SJ.
Gene expression databases
ArrayExpress	P28235.
Bgee	P28235.
CleanEx	MM_GJA4.
Genevestigator	P28235.
GermOnline	ENSMUSG00000050234. Mus musculus.
Family and domain databases
InterPro	IPR000500. Connexin. IPR002263. Connexin37. IPR019570. Connexin_CCC. IPR017990. Connexin_CS. IPR013092. Connexin_N. [Graphical view]
PANTHER	PTHR11984. PTHR11984. 1 hit. PTHR11984:SF17. PTHR11984:SF17. 1 hit.
Pfam	PF00029. Connexin. 1 hit. PF10582. Connexin_CCC. 1 hit. [Graphical view]
PRINTS	PR00206. CONNEXIN. PR01134. CONNEXINA4.
SMART	SM00037. CNX. 1 hit. SM01089. Connexin_CCC. 1 hit. [Graphical view]
PROSITE	PS00407. CONNEXINS_1. 1 hit. PS00408. CONNEXINS_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	GJA4. mouse.
NextBio	286416.
SOURCE	Search...

Entry information

Entry name

CXA4_MOUSE

Accession

Primary (citable) accession number: P28235

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 101 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program