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P28234

- CXA5_RAT

UniProt

P28234 - CXA5_RAT

Protein

Gap junction alpha-5 protein

Gene

Gja5

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.

    GO - Molecular functioni

    1. connexin binding Source: RGD
    2. gap junction channel activity Source: RGD
    3. gap junction channel activity involved in AV node cell-bundle of His cell electrical coupling Source: Ensembl
    4. gap junction hemi-channel activity Source: Ensembl

    GO - Biological processi

    1. angiogenesis Source: Ensembl
    2. artery morphogenesis Source: Ensembl
    3. atrial cardiac muscle cell action potential Source: Ensembl
    4. atrial septum morphogenesis Source: Ensembl
    5. atrial ventricular junction remodeling Source: Ensembl
    6. bundle of His cell to Purkinje myocyte communication Source: Ensembl
    7. cardiac conduction system development Source: Ensembl
    8. cell communication by chemical coupling Source: RGD
    9. cell communication by electrical coupling Source: RGD
    10. embryonic heart tube development Source: Ensembl
    11. embryonic limb morphogenesis Source: Ensembl
    12. endothelium development Source: RGD
    13. foramen ovale closure Source: Ensembl
    14. gap junction assembly Source: Ensembl
    15. heart development Source: RGD
    16. mitral valve development Source: Ensembl
    17. negative regulation of blood pressure Source: RGD
    18. negative regulation of glomerular filtration Source: RGD
    19. outflow tract morphogenesis Source: Ensembl
    20. positive regulation of cell communication by chemical coupling Source: RGD
    21. positive regulation of vasoconstriction Source: RGD
    22. positive regulation of vasodilation Source: RGD
    23. potassium ion transport Source: RGD
    24. protein oligomerization Source: RGD
    25. pulmonary valve formation Source: Ensembl
    26. regulation of atrial cardiac muscle cell membrane depolarization Source: Ensembl
    27. regulation of heart rate by cardiac conduction Source: Ensembl
    28. regulation of membrane depolarization during cardiac muscle cell action potential Source: Ensembl
    29. regulation of renin secretion into blood stream Source: Ensembl
    30. regulation of ventricular cardiac muscle cell membrane depolarization Source: Ensembl
    31. regulation of ventricular cardiac muscle cell membrane repolarization Source: Ensembl
    32. SA node cell action potential Source: Ensembl
    33. septum primum development Source: Ensembl
    34. skeletal system development Source: Ensembl
    35. transmembrane transport Source: GOC
    36. vasomotion Source: RGD
    37. ventricular cardiac muscle cell action potential Source: Ensembl
    38. ventricular septum morphogenesis Source: Ensembl

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Gap junction alpha-5 protein
    Alternative name(s):
    Connexin-40
    Short name:
    Cx40
    Gene namesi
    Name:Gja5
    Synonyms:Cxn-40
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 2

    Organism-specific databases

    RGDi2692. Gja5.

    Subcellular locationi

    GO - Cellular componenti

    1. cell projection Source: RGD
    2. connexon complex Source: RGD
    3. integral component of plasma membrane Source: Ensembl
    4. intercalated disc Source: Ensembl

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Gap junction, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 356356Gap junction alpha-5 proteinPRO_0000057821Add
    BLAST

    Proteomic databases

    PRIDEiP28234.

    Expressioni

    Tissue specificityi

    Highly expressed in lung.

    Gene expression databases

    GenevestigatoriP28234.

    Interactioni

    Subunit structurei

    A connexon is composed of a hexamer of connexins.

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000023488.

    Structurei

    Secondary structure

    1
    356
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi261 – 2633
    Turni266 – 2694
    Beta strandi293 – 2975
    Beta strandi300 – 3034
    Beta strandi323 – 3253
    Beta strandi327 – 3293
    Turni332 – 3343

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2K7MNMR-A252-356[»]
    ProteinModelPortaliP28234.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP28234.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1919CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini41 – 7636ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini98 – 16366CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini185 – 20420ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini226 – 356131CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei20 – 4021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei77 – 9721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei164 – 18421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei205 – 22521HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG45265.
    GeneTreeiENSGT00740000115285.
    HOGENOMiHOG000231127.
    HOVERGENiHBG009576.
    InParanoidiP28234.
    KOiK07614.
    OMAiDWSFLGE.
    OrthoDBiEOG7P2XSS.
    PhylomeDBiP28234.
    TreeFamiTF329606.

    Family and domain databases

    InterProiIPR000500. Connexin.
    IPR002264. Connexin40.
    IPR019570. Connexin_CCC.
    IPR017990. Connexin_CS.
    IPR013092. Connexin_N.
    [Graphical view]
    PANTHERiPTHR11984. PTHR11984. 1 hit.
    PTHR11984:SF13. PTHR11984:SF13. 1 hit.
    PfamiPF00029. Connexin. 1 hit.
    PF10582. Connexin_CCC. 1 hit.
    [Graphical view]
    PRINTSiPR00206. CONNEXIN.
    PR01135. CONNEXINA5.
    SMARTiSM00037. CNX. 1 hit.
    SM01089. Connexin_CCC. 1 hit.
    [Graphical view]
    PROSITEiPS00407. CONNEXINS_1. 1 hit.
    PS00408. CONNEXINS_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P28234-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGDWSFLGEF LEEVHKHSTV IGKVWLTVLF IFRMLVLGTA AESSWGDEQA    50
    DFRCDTIQPG CQNVCYDQAF PISHIRYWVL QIIFVSTPSL VYMGHAMHTV 100
    RMQEKQKLRE AEKAKEAGGT GTYEYLAEKA ELSCWKEVNG KIVLQGTLLN 150
    TYVCTILIRT AMEVAFMVGQ YLLYGIFLDT LHVCRRSPCP HPVNCYVSRP 200
    TEKNVFIVFM MAVAGLSLFL SLAELYHLGW KKIRQRLAKS RQGDKHQLLG 250
    PSTSLVQGLT PPPDFNQCLK NSPDEKFFSD FSNNMGSRKN PDPLATEEVP 300
    NQEQIPEEGF IHTQYGQKPE QPSGASAGHR FPQGYHSDKR RLSKASSKAR 350
    SDDLSV 356
    Length:356
    Mass (Da):40,233
    Last modified:January 23, 2007 - v2
    Checksum:i7D459FE69D9ACF0C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M76535 Genomic DNA. Translation: AAA41000.1.
    M83092 mRNA. Translation: AAA41194.1.
    AF021806 mRNA. Translation: AAC53502.1.
    AF022136 Genomic DNA. Translation: AAC53503.1.
    BC070935 mRNA. Translation: AAH70935.1.
    PIRiA42053.
    RefSeqiNP_062153.1. NM_019280.1.
    UniGeneiRn.88300.

    Genome annotation databases

    EnsembliENSRNOT00000023488; ENSRNOP00000023488; ENSRNOG00000017484.
    GeneIDi50563.
    KEGGirno:50563.
    UCSCiRGD:2692. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M76535 Genomic DNA. Translation: AAA41000.1 .
    M83092 mRNA. Translation: AAA41194.1 .
    AF021806 mRNA. Translation: AAC53502.1 .
    AF022136 Genomic DNA. Translation: AAC53503.1 .
    BC070935 mRNA. Translation: AAH70935.1 .
    PIRi A42053.
    RefSeqi NP_062153.1. NM_019280.1.
    UniGenei Rn.88300.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2K7M NMR - A 252-356 [» ]
    ProteinModelPortali P28234.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000023488.

    Proteomic databases

    PRIDEi P28234.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000023488 ; ENSRNOP00000023488 ; ENSRNOG00000017484 .
    GeneIDi 50563.
    KEGGi rno:50563.
    UCSCi RGD:2692. rat.

    Organism-specific databases

    CTDi 2702.
    RGDi 2692. Gja5.

    Phylogenomic databases

    eggNOGi NOG45265.
    GeneTreei ENSGT00740000115285.
    HOGENOMi HOG000231127.
    HOVERGENi HBG009576.
    InParanoidi P28234.
    KOi K07614.
    OMAi DWSFLGE.
    OrthoDBi EOG7P2XSS.
    PhylomeDBi P28234.
    TreeFami TF329606.

    Miscellaneous databases

    EvolutionaryTracei P28234.
    NextBioi 610390.
    PROi P28234.

    Gene expression databases

    Genevestigatori P28234.

    Family and domain databases

    InterProi IPR000500. Connexin.
    IPR002264. Connexin40.
    IPR019570. Connexin_CCC.
    IPR017990. Connexin_CS.
    IPR013092. Connexin_N.
    [Graphical view ]
    PANTHERi PTHR11984. PTHR11984. 1 hit.
    PTHR11984:SF13. PTHR11984:SF13. 1 hit.
    Pfami PF00029. Connexin. 1 hit.
    PF10582. Connexin_CCC. 1 hit.
    [Graphical view ]
    PRINTSi PR00206. CONNEXIN.
    PR01135. CONNEXINA5.
    SMARTi SM00037. CNX. 1 hit.
    SM01089. Connexin_CCC. 1 hit.
    [Graphical view ]
    PROSITEi PS00407. CONNEXINS_1. 1 hit.
    PS00408. CONNEXINS_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Four novel members of the connexin family of gap junction proteins. Molecular cloning, expression, and chromosome mapping."
      Haefliger J.-A., Bruzzone R., Jenkins N.A., Gilbert D.J., Copeland N.G., Paul D.L.
      J. Biol. Chem. 267:2057-2064(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: Sprague-Dawley.
    2. "Molecular cloning and expression of rat connexin40, a gap junction protein expressed in vascular smooth muscle."
      Beyer E.C., Reed K.E., Westphale E.M., Kanter H.L., Larson D.M.
      J. Membr. Biol. 127:69-76(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Mouse connexin40: gene structure and promoter analysis."
      Seul K.H., Tadros P.N., Beyer E.C.
      Genomics 46:120-126(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Lung.

    Entry informationi

    Entry nameiCXA5_RAT
    AccessioniPrimary (citable) accession number: P28234
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 109 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3