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Protein

Gap junction alpha-5 protein

Gene

Gja5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Gap junction alpha-5 protein
Alternative name(s):
Connexin-40
Short name:
Cx40
Gene namesi
Name:Gja5
Synonyms:Cxn-40
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi2692. Gja5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1919CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei20 – 4021HelicalSequence AnalysisAdd
BLAST
Topological domaini41 – 7636ExtracellularSequence AnalysisAdd
BLAST
Transmembranei77 – 9721HelicalSequence AnalysisAdd
BLAST
Topological domaini98 – 16366CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei164 – 18421HelicalSequence AnalysisAdd
BLAST
Topological domaini185 – 20420ExtracellularSequence AnalysisAdd
BLAST
Transmembranei205 – 22521HelicalSequence AnalysisAdd
BLAST
Topological domaini226 – 356131CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • cell projection Source: RGD
  • connexon complex Source: RGD
  • integral component of plasma membrane Source: Ensembl
  • intercalated disc Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Gap junction, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 356356Gap junction alpha-5 proteinPRO_0000057821Add
BLAST

Proteomic databases

PRIDEiP28234.

Expressioni

Tissue specificityi

Highly expressed in lung.

Gene expression databases

GenevisibleiP28234. RN.

Interactioni

Subunit structurei

A connexon is composed of a hexamer of connexins.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000023488.

Structurei

Secondary structure

1
356
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi261 – 2633Combined sources
Turni266 – 2694Combined sources
Beta strandi293 – 2975Combined sources
Beta strandi300 – 3034Combined sources
Beta strandi323 – 3253Combined sources
Beta strandi327 – 3293Combined sources
Turni332 – 3343Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2K7MNMR-A252-356[»]
ProteinModelPortaliP28234.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP28234.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG45265.
GeneTreeiENSGT00800000124029.
HOGENOMiHOG000231127.
HOVERGENiHBG009576.
InParanoidiP28234.
KOiK07614.
OMAiHKHSTVI.
OrthoDBiEOG7P2XSS.
PhylomeDBiP28234.
TreeFamiTF329606.

Family and domain databases

InterProiIPR000500. Connexin.
IPR002264. Connexin40.
IPR019570. Connexin_CCC.
IPR017990. Connexin_CS.
IPR013092. Connexin_N.
[Graphical view]
PANTHERiPTHR11984. PTHR11984. 1 hit.
PTHR11984:SF13. PTHR11984:SF13. 1 hit.
PfamiPF00029. Connexin. 1 hit.
PF10582. Connexin_CCC. 1 hit.
[Graphical view]
PRINTSiPR00206. CONNEXIN.
PR01135. CONNEXINA5.
SMARTiSM00037. CNX. 1 hit.
SM01089. Connexin_CCC. 1 hit.
[Graphical view]
PROSITEiPS00407. CONNEXINS_1. 1 hit.
PS00408. CONNEXINS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P28234-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDWSFLGEF LEEVHKHSTV IGKVWLTVLF IFRMLVLGTA AESSWGDEQA
60 70 80 90 100
DFRCDTIQPG CQNVCYDQAF PISHIRYWVL QIIFVSTPSL VYMGHAMHTV
110 120 130 140 150
RMQEKQKLRE AEKAKEAGGT GTYEYLAEKA ELSCWKEVNG KIVLQGTLLN
160 170 180 190 200
TYVCTILIRT AMEVAFMVGQ YLLYGIFLDT LHVCRRSPCP HPVNCYVSRP
210 220 230 240 250
TEKNVFIVFM MAVAGLSLFL SLAELYHLGW KKIRQRLAKS RQGDKHQLLG
260 270 280 290 300
PSTSLVQGLT PPPDFNQCLK NSPDEKFFSD FSNNMGSRKN PDPLATEEVP
310 320 330 340 350
NQEQIPEEGF IHTQYGQKPE QPSGASAGHR FPQGYHSDKR RLSKASSKAR

SDDLSV
Length:356
Mass (Da):40,233
Last modified:January 23, 2007 - v2
Checksum:i7D459FE69D9ACF0C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M76535 Genomic DNA. Translation: AAA41000.1.
M83092 mRNA. Translation: AAA41194.1.
AF021806 mRNA. Translation: AAC53502.1.
AF022136 Genomic DNA. Translation: AAC53503.1.
BC070935 mRNA. Translation: AAH70935.1.
PIRiA42053.
RefSeqiNP_062153.1. NM_019280.1.
XP_008759550.1. XM_008761328.1.
UniGeneiRn.88300.

Genome annotation databases

EnsembliENSRNOT00000023488; ENSRNOP00000023488; ENSRNOG00000017484.
GeneIDi50563.
KEGGirno:50563.
UCSCiRGD:2692. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M76535 Genomic DNA. Translation: AAA41000.1.
M83092 mRNA. Translation: AAA41194.1.
AF021806 mRNA. Translation: AAC53502.1.
AF022136 Genomic DNA. Translation: AAC53503.1.
BC070935 mRNA. Translation: AAH70935.1.
PIRiA42053.
RefSeqiNP_062153.1. NM_019280.1.
XP_008759550.1. XM_008761328.1.
UniGeneiRn.88300.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2K7MNMR-A252-356[»]
ProteinModelPortaliP28234.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000023488.

Proteomic databases

PRIDEiP28234.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000023488; ENSRNOP00000023488; ENSRNOG00000017484.
GeneIDi50563.
KEGGirno:50563.
UCSCiRGD:2692. rat.

Organism-specific databases

CTDi2702.
RGDi2692. Gja5.

Phylogenomic databases

eggNOGiNOG45265.
GeneTreeiENSGT00800000124029.
HOGENOMiHOG000231127.
HOVERGENiHBG009576.
InParanoidiP28234.
KOiK07614.
OMAiHKHSTVI.
OrthoDBiEOG7P2XSS.
PhylomeDBiP28234.
TreeFamiTF329606.

Miscellaneous databases

EvolutionaryTraceiP28234.
NextBioi610390.
PROiP28234.

Gene expression databases

GenevisibleiP28234. RN.

Family and domain databases

InterProiIPR000500. Connexin.
IPR002264. Connexin40.
IPR019570. Connexin_CCC.
IPR017990. Connexin_CS.
IPR013092. Connexin_N.
[Graphical view]
PANTHERiPTHR11984. PTHR11984. 1 hit.
PTHR11984:SF13. PTHR11984:SF13. 1 hit.
PfamiPF00029. Connexin. 1 hit.
PF10582. Connexin_CCC. 1 hit.
[Graphical view]
PRINTSiPR00206. CONNEXIN.
PR01135. CONNEXINA5.
SMARTiSM00037. CNX. 1 hit.
SM01089. Connexin_CCC. 1 hit.
[Graphical view]
PROSITEiPS00407. CONNEXINS_1. 1 hit.
PS00408. CONNEXINS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Four novel members of the connexin family of gap junction proteins. Molecular cloning, expression, and chromosome mapping."
    Haefliger J.-A., Bruzzone R., Jenkins N.A., Gilbert D.J., Copeland N.G., Paul D.L.
    J. Biol. Chem. 267:2057-2064(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: Sprague-Dawley.
  2. "Molecular cloning and expression of rat connexin40, a gap junction protein expressed in vascular smooth muscle."
    Beyer E.C., Reed K.E., Westphale E.M., Kanter H.L., Larson D.M.
    J. Membr. Biol. 127:69-76(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Mouse connexin40: gene structure and promoter analysis."
    Seul K.H., Tadros P.N., Beyer E.C.
    Genomics 46:120-126(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.

Entry informationi

Entry nameiCXA5_RAT
AccessioniPrimary (citable) accession number: P28234
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: January 23, 2007
Last modified: July 22, 2015
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.