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P28234 (CXA5_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Gap junction alpha-5 protein
Alternative name(s):
Connexin-40
Short name=Cx40
Gene names
Name:Gja5
Synonyms:Cxn-40
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length356 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.

Subunit structure

A connexon is composed of a hexamer of connexins.

Subcellular location

Cell membrane; Multi-pass membrane protein. Cell junctiongap junction.

Tissue specificity

Highly expressed in lung.

Sequence similarities

Belongs to the connexin family. Alpha-type (group II) subfamily.

Ontologies

Keywords
   Cellular componentCell junction
Cell membrane
Gap junction
Membrane
   DomainTransmembrane
Transmembrane helix
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processSA node cell action potential

Inferred from electronic annotation. Source: Ensembl

angiogenesis

Inferred from electronic annotation. Source: Ensembl

artery morphogenesis

Inferred from electronic annotation. Source: Ensembl

atrial cardiac muscle cell action potential

Inferred from electronic annotation. Source: Ensembl

atrial septum morphogenesis

Inferred from electronic annotation. Source: Ensembl

atrial ventricular junction remodeling

Inferred from electronic annotation. Source: Ensembl

bundle of His cell to Purkinje myocyte communication

Inferred from electronic annotation. Source: Ensembl

cardiac conduction system development

Inferred from electronic annotation. Source: Ensembl

cell communication by chemical coupling

Inferred from direct assay Ref.2. Source: RGD

cell communication by electrical coupling

Inferred from direct assay PubMed 7554128. Source: RGD

embryonic heart tube development

Inferred from electronic annotation. Source: Ensembl

embryonic limb morphogenesis

Inferred from electronic annotation. Source: Ensembl

endothelium development

Inferred from expression pattern PubMed 10990491. Source: RGD

foramen ovale closure

Inferred from electronic annotation. Source: Ensembl

gap junction assembly

Inferred from electronic annotation. Source: Ensembl

heart development

Inferred from expression pattern PubMed 8944820. Source: RGD

mitral valve development

Inferred from electronic annotation. Source: Ensembl

negative regulation of blood pressure

Inferred from mutant phenotype PubMed 18046320. Source: RGD

negative regulation of glomerular filtration

Inferred from mutant phenotype PubMed 17928514. Source: RGD

outflow tract morphogenesis

Inferred from electronic annotation. Source: Ensembl

positive regulation of cell communication by chemical coupling

Inferred from mutant phenotype PubMed 15678088. Source: RGD

positive regulation of vasoconstriction

Inferred from mutant phenotype PubMed 21414209. Source: RGD

positive regulation of vasodilation

Inferred from mutant phenotype PubMed 16037574. Source: RGD

potassium ion transport

Inferred from direct assay PubMed 7554128. Source: RGD

protein oligomerization

Inferred from direct assay PubMed 11557558. Source: RGD

pulmonary valve formation

Inferred from electronic annotation. Source: Ensembl

regulation of atrial cardiac muscle cell membrane depolarization

Inferred from electronic annotation. Source: Ensembl

regulation of heart rate by cardiac conduction

Inferred from electronic annotation. Source: Ensembl

regulation of membrane depolarization during cardiac muscle cell action potential

Inferred from electronic annotation. Source: Ensembl

regulation of renin secretion into blood stream

Inferred from electronic annotation. Source: Ensembl

regulation of ventricular cardiac muscle cell membrane depolarization

Inferred from electronic annotation. Source: Ensembl

regulation of ventricular cardiac muscle cell membrane repolarization

Inferred from electronic annotation. Source: Ensembl

septum primum development

Inferred from electronic annotation. Source: Ensembl

skeletal system development

Inferred from electronic annotation. Source: Ensembl

transmembrane transport

Inferred from direct assay Ref.2. Source: GOC

vasomotion

Inferred from mutant phenotype PubMed 16815985. Source: RGD

ventricular cardiac muscle cell action potential

Inferred from electronic annotation. Source: Ensembl

ventricular septum morphogenesis

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcell projection

Inferred from direct assay PubMed 16037574. Source: RGD

connexon complex

Inferred from direct assay Ref.2PubMed 9851942. Source: RGD

integral component of plasma membrane

Inferred from electronic annotation. Source: Ensembl

intercalated disc

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionconnexin binding

Inferred from physical interaction PubMed 11557558. Source: RGD

gap junction channel activity

Inferred from direct assay Ref.2. Source: RGD

gap junction channel activity involved in AV node cell-bundle of His cell electrical coupling

Inferred from electronic annotation. Source: Ensembl

gap junction hemi-channel activity

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 356356Gap junction alpha-5 protein
PRO_0000057821

Regions

Topological domain1 – 1919Cytoplasmic Potential
Transmembrane20 – 4021Helical; Potential
Topological domain41 – 7636Extracellular Potential
Transmembrane77 – 9721Helical; Potential
Topological domain98 – 16366Cytoplasmic Potential
Transmembrane164 – 18421Helical; Potential
Topological domain185 – 20420Extracellular Potential
Transmembrane205 – 22521Helical; Potential
Topological domain226 – 356131Cytoplasmic Potential

Secondary structure

............... 356
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P28234 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 7D459FE69D9ACF0C

FASTA35640,233
        10         20         30         40         50         60 
MGDWSFLGEF LEEVHKHSTV IGKVWLTVLF IFRMLVLGTA AESSWGDEQA DFRCDTIQPG 

        70         80         90        100        110        120 
CQNVCYDQAF PISHIRYWVL QIIFVSTPSL VYMGHAMHTV RMQEKQKLRE AEKAKEAGGT 

       130        140        150        160        170        180 
GTYEYLAEKA ELSCWKEVNG KIVLQGTLLN TYVCTILIRT AMEVAFMVGQ YLLYGIFLDT 

       190        200        210        220        230        240 
LHVCRRSPCP HPVNCYVSRP TEKNVFIVFM MAVAGLSLFL SLAELYHLGW KKIRQRLAKS 

       250        260        270        280        290        300 
RQGDKHQLLG PSTSLVQGLT PPPDFNQCLK NSPDEKFFSD FSNNMGSRKN PDPLATEEVP 

       310        320        330        340        350 
NQEQIPEEGF IHTQYGQKPE QPSGASAGHR FPQGYHSDKR RLSKASSKAR SDDLSV 

« Hide

References

« Hide 'large scale' references
[1]"Four novel members of the connexin family of gap junction proteins. Molecular cloning, expression, and chromosome mapping."
Haefliger J.-A., Bruzzone R., Jenkins N.A., Gilbert D.J., Copeland N.G., Paul D.L.
J. Biol. Chem. 267:2057-2064(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: Sprague-Dawley.
[2]"Molecular cloning and expression of rat connexin40, a gap junction protein expressed in vascular smooth muscle."
Beyer E.C., Reed K.E., Westphale E.M., Kanter H.L., Larson D.M.
J. Membr. Biol. 127:69-76(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Mouse connexin40: gene structure and promoter analysis."
Seul K.H., Tadros P.N., Beyer E.C.
Genomics 46:120-126(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Lung.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M76535 Genomic DNA. Translation: AAA41000.1.
M83092 mRNA. Translation: AAA41194.1.
AF021806 mRNA. Translation: AAC53502.1.
AF022136 Genomic DNA. Translation: AAC53503.1.
BC070935 mRNA. Translation: AAH70935.1.
PIRA42053.
RefSeqNP_062153.1. NM_019280.1.
UniGeneRn.88300.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2K7MNMR-A252-356[»]
ProteinModelPortalP28234.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000023488.

Proteomic databases

PRIDEP28234.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000023488; ENSRNOP00000023488; ENSRNOG00000017484.
GeneID50563.
KEGGrno:50563.
UCSCRGD:2692. rat.

Organism-specific databases

CTD2702.
RGD2692. Gja5.

Phylogenomic databases

eggNOGNOG45265.
GeneTreeENSGT00740000115285.
HOGENOMHOG000231127.
HOVERGENHBG009576.
InParanoidP28234.
KOK07614.
OMADWSFLGE.
OrthoDBEOG7P2XSS.
PhylomeDBP28234.
TreeFamTF329606.

Gene expression databases

GenevestigatorP28234.

Family and domain databases

InterProIPR000500. Connexin.
IPR002264. Connexin40.
IPR019570. Connexin_CCC.
IPR017990. Connexin_CS.
IPR013092. Connexin_N.
[Graphical view]
PANTHERPTHR11984. PTHR11984. 1 hit.
PTHR11984:SF13. PTHR11984:SF13. 1 hit.
PfamPF00029. Connexin. 1 hit.
PF10582. Connexin_CCC. 1 hit.
[Graphical view]
PRINTSPR00206. CONNEXIN.
PR01135. CONNEXINA5.
SMARTSM00037. CNX. 1 hit.
SM01089. Connexin_CCC. 1 hit.
[Graphical view]
PROSITEPS00407. CONNEXINS_1. 1 hit.
PS00408. CONNEXINS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP28234.
NextBio610390.
PROP28234.

Entry information

Entry nameCXA5_RAT
AccessionPrimary (citable) accession number: P28234
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references