Reviewed, UniProtKB/Swiss-Prot P28234 (CXA5_RAT)

Names and origin

Protein names

Recommended name:
Gap junction alpha-5 protein

Alternative name(s):
Connexin-40
Short name=Cx40

Gene names

Name:	Gja5
Synonyms:	Cxn-40

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
Subunit structure	A connexon is composed of a hexamer of connexins.
Subcellular location	Cell membrane; Multi-pass membrane protein. Cell junction › gap junction.
Tissue specificity	Highly expressed in lung.
Sequence similarities	Belongs to the connexin family. Alpha-type (group II) subfamily.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Gap junction Membrane
Domain	Transmembrane
Technical term	3D-structure
Gene Ontology (GO)
Biological process	cell-cell signaling Ref.2 Inferred from physical interaction. Source: RGD
Cellular component	connexon complex Ref.2 Inferred from direct assay. Source: RGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	gap junction channel activity Ref.2 Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 356	355	Gap junction alpha-5 protein		PRO_0000057821
Regions
	Topological domain	2 – 19	18	Cytoplasmic Potential
	Transmembrane	20 – 40	21	Potential
	Topological domain	41 – 76	36	Extracellular Potential
	Transmembrane	77 – 97	21	Potential
	Topological domain	98 – 163	66	Cytoplasmic Potential
	Transmembrane	164 – 184	21	Potential
	Topological domain	185 – 204	20	Extracellular Potential
	Transmembrane	205 – 225	21	Potential
	Topological domain	226 – 356	131	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	P28234-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 7D459FE69D9ACF0C	FASTA	356	40,233
	10 20 30 40 50 60 MGDWSFLGEF LEEVHKHSTV IGKVWLTVLF IFRMLVLGTA AESSWGDEQA DFRCDTIQPG 70 80 90 100 110 120 CQNVCYDQAF PISHIRYWVL QIIFVSTPSL VYMGHAMHTV RMQEKQKLRE AEKAKEAGGT 130 140 150 160 170 180 GTYEYLAEKA ELSCWKEVNG KIVLQGTLLN TYVCTILIRT AMEVAFMVGQ YLLYGIFLDT 190 200 210 220 230 240 LHVCRRSPCP HPVNCYVSRP TEKNVFIVFM MAVAGLSLFL SLAELYHLGW KKIRQRLAKS 250 260 270 280 290 300 RQGDKHQLLG PSTSLVQGLT PPPDFNQCLK NSPDEKFFSD FSNNMGSRKN PDPLATEEVP 310 320 330 340 350 NQEQIPEEGF IHTQYGQKPE QPSGASAGHR FPQGYHSDKR RLSKASSKAR SDDLSV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Four novel members of the connexin family of gap junction proteins. Molecular cloning, expression, and chromosome mapping." Haefliger J.-A., Bruzzone R., Jenkins N.A., Gilbert D.J., Copeland N.G., Paul D.L. J. Biol. Chem. 267:2057-2064(1992) [PubMed: 1370487] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: Sprague-Dawley.
[2]	"Molecular cloning and expression of rat connexin40, a gap junction protein expressed in vascular smooth muscle." Beyer E.C., Reed K.E., Westphale E.M., Kanter H.L., Larson D.M. J. Membr. Biol. 127:69-76(1992) [PubMed: 1328644] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Mouse connexin40: gene structure and promoter analysis." Seul K.H., Tadros P.N., Beyer E.C. Genomics 46:120-126(1997) [PubMed: 9403066] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M76535 Genomic DNA. Translation: AAA41000.1.
M83092 mRNA. Translation: AAA41194.1.
AF021806 mRNA. Translation: AAC53502.1.
AF022136 Genomic DNA. Translation: AAC53503.1.
BC070935 mRNA. Translation: AAH70935.1.

IPI

IPI00230782.

PIR

A42053.

RefSeq

NP_062153.1.

UniGene

Rn.88300

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2K7M	NMR	-	A	252-356	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

P28234.

Genome annotation databases

Ensembl

ENSRNOT00000023488; ENSRNOP00000023488; ENSRNOG00000017484; Rattus norvegicus. [Genome view]

GeneID

50563.

KEGG

rno:50563.

UCSC

NM_019280. rat.

Organism-specific databases

CTD

50563.

RGD

2692. Gja5.

Phylogenomic databases

eggNOG

roNOG11929.

HOVERGEN

HBG009576.

InParanoid

P28234.

OMA

FLGEFLE.

OrthoDB

EOG9D55GZ.

PhylomeDB

P28234.

Gene expression databases

ArrayExpress

P28234.

Genevestigator

P28234.

GermOnline

ENSRNOG00000017484. Rattus norvegicus.

Family and domain databases

InterPro

IPR000500. Connexin.
IPR002264. Connexin40.
IPR017992. Connexin40_cons-rgn.
IPR019570. Connexin_CCC.
IPR017990. Connexin_CS.
IPR013092. Connexin_N.
IPR017991. connexins_rgn.
[Graphical view]

PANTHER

PTHR11984. Connexin. 1 hit.
PTHR11984:SF13. Connexin40. 1 hit.

Pfam

PF00029. Connexin. 1 hit.
PF10582. Connexin_CCC. 1 hit.
[Graphical view]

PRINTS

PR00206. CONNEXIN.
PR01135. CONNEXINA5.

SMART

SM00037. CNX. 1 hit.
[Graphical view]

PROSITE

PS00407. CONNEXINS_1. 1 hit.
PS00408. CONNEXINS_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

610390.

Entry information

Entry name

CXA5_RAT

Accession

Primary (citable) accession number: P28234

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	January 23, 2007
Last modified:	March 2, 2010
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)