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Protein

Gap junction beta-1 protein

Gene

Gjb1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.

GO - Molecular functioni

  • gap junction channel activity Source: Ensembl
  • protein homodimerization activity Source: MGI

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-190704. Oligomerization of connexins into connexons.
R-MMU-190861. Gap junction assembly.

Names & Taxonomyi

Protein namesi
Recommended name:
Gap junction beta-1 protein
Alternative name(s):
Connexin-32
Short name:
Cx32
Gene namesi
Name:Gjb1
Synonyms:Cxn-32
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:95719. Gjb1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2222CytoplasmicSequence analysisAdd
BLAST
Transmembranei23 – 4523HelicalSequence analysisAdd
BLAST
Topological domaini46 – 7530ExtracellularSequence analysisAdd
BLAST
Transmembranei76 – 9520HelicalSequence analysisAdd
BLAST
Topological domaini96 – 13035CytoplasmicSequence analysisAdd
BLAST
Transmembranei131 – 15323HelicalSequence analysisAdd
BLAST
Topological domaini154 – 19138ExtracellularSequence analysisAdd
BLAST
Transmembranei192 – 21423HelicalSequence analysisAdd
BLAST
Topological domaini215 – 28369CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • connexon complex Source: InterPro
  • cytoplasm Source: MGI
  • endoplasmic reticulum Source: Ensembl
  • gap junction Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Gap junction, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice lacking both Gja12 and Gjb1 display a severe demyelination phenotype associated with oligodendrocyte death. These mice develop action tremors, tonic seizures, sporadic convulsions and loss of consciousness preceding death in the sixth week after birth.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 283283Gap junction beta-1 proteinPRO_0000057851Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei233 – 2331PhosphoserineBy similarity
Modified residuei258 – 2581PhosphoserineCombined sources
Modified residuei266 – 2661PhosphoserineCombined sources
Modified residuei277 – 2771PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP28230.
PaxDbiP28230.
PRIDEiP28230.

PTM databases

iPTMnetiP28230.
PhosphoSiteiP28230.

Miscellaneous databases

PMAP-CutDBP28230.

Expressioni

Gene expression databases

BgeeiP28230.
CleanExiMM_GJB1.
GenevisibleiP28230. MM.

Interactioni

Subunit structurei

A connexon is composed of a hexamer of connexins. Interacts with CNST.1 Publication

GO - Molecular functioni

  • protein homodimerization activity Source: MGI

Protein-protein interaction databases

IntActiP28230. 1 interaction.
STRINGi10090.ENSMUSP00000062723.

Structurei

3D structure databases

ProteinModelPortaliP28230.
SMRiP28230. Positions 2-215.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IFM8. Eukaryota.
ENOG410Y7VN. LUCA.
GeneTreeiENSGT00840000129674.
HOGENOMiHOG000231127.
HOVERGENiHBG009576.
InParanoidiP28230.
KOiK07620.
OMAiYLVVRAC.
OrthoDBiEOG7P2XSS.
PhylomeDBiP28230.
TreeFamiTF329606.

Family and domain databases

InterProiIPR000500. Connexin.
IPR002267. Connexin32.
IPR019570. Connexin_CCC.
IPR017990. Connexin_CS.
IPR013092. Connexin_N.
[Graphical view]
PANTHERiPTHR11984. PTHR11984. 1 hit.
PfamiPF00029. Connexin. 1 hit.
[Graphical view]
PRINTSiPR00206. CONNEXIN.
PR01138. CONNEXINB1.
SMARTiSM00037. CNX. 1 hit.
SM01089. Connexin_CCC. 1 hit.
[Graphical view]
PROSITEiPS00407. CONNEXINS_1. 1 hit.
PS00408. CONNEXINS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P28230-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNWTGLYTLL SGVNRHSTAI GRVWLSVIFI FRIMVLVVAA ESVWGDEKSS
60 70 80 90 100
FICNTLQPGC NSVCYDHFFP ISHVRLWSLQ LILVSTPALL VAMHVAHQQH
110 120 130 140 150
IEKKMLRLEG HGDPLHLEEV KRHKVHISGT LWWTYVISVV FRLLFEAVFM
160 170 180 190 200
YVFYLLYPGY AMVRLVKCEA FPCPNTVDCF VSRPTEKTVF TVFMLAASGI
210 220 230 240 250
CIILNVAEVV YLIIRACARR AQRRSNPPSR KGSGFGHRLS PEYKQNEINK
260 270 280
LLSEQDGSLK DILRRSPGTG AGLAEKSDRC SAC
Length:283
Mass (Da):32,004
Last modified:May 10, 2004 - v3
Checksum:iC79FC46AA13BC5D7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti235 – 2351F → S in AAA37296 (PubMed:2060697).Curated
Sequence conflicti263 – 2664LRRS → PXPH in AAA37296 (PubMed:2060697).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63802 mRNA. Translation: AAA37296.2.
M81447 Genomic DNA. Translation: AAA37496.1.
AJ271753 Genomic DNA. Translation: CAB72446.1.
BC026833 mRNA. Translation: AAH26833.1.
CCDSiCCDS30314.1.
PIRiB49769.
RefSeqiNP_001289425.1. NM_001302496.1.
NP_001289426.1. NM_001302497.1.
NP_001289427.1. NM_001302498.1.
NP_032150.2. NM_008124.3.
UniGeneiMm.21198.

Genome annotation databases

EnsembliENSMUST00000052130; ENSMUSP00000062723; ENSMUSG00000047797.
ENSMUST00000119080; ENSMUSP00000113904; ENSMUSG00000047797.
ENSMUST00000119190; ENSMUSP00000113516; ENSMUSG00000047797.
GeneIDi14618.
KEGGimmu:14618.
UCSCiuc009txk.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63802 mRNA. Translation: AAA37296.2.
M81447 Genomic DNA. Translation: AAA37496.1.
AJ271753 Genomic DNA. Translation: CAB72446.1.
BC026833 mRNA. Translation: AAH26833.1.
CCDSiCCDS30314.1.
PIRiB49769.
RefSeqiNP_001289425.1. NM_001302496.1.
NP_001289426.1. NM_001302497.1.
NP_001289427.1. NM_001302498.1.
NP_032150.2. NM_008124.3.
UniGeneiMm.21198.

3D structure databases

ProteinModelPortaliP28230.
SMRiP28230. Positions 2-215.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP28230. 1 interaction.
STRINGi10090.ENSMUSP00000062723.

PTM databases

iPTMnetiP28230.
PhosphoSiteiP28230.

Proteomic databases

MaxQBiP28230.
PaxDbiP28230.
PRIDEiP28230.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000052130; ENSMUSP00000062723; ENSMUSG00000047797.
ENSMUST00000119080; ENSMUSP00000113904; ENSMUSG00000047797.
ENSMUST00000119190; ENSMUSP00000113516; ENSMUSG00000047797.
GeneIDi14618.
KEGGimmu:14618.
UCSCiuc009txk.2. mouse.

Organism-specific databases

CTDi2705.
MGIiMGI:95719. Gjb1.

Phylogenomic databases

eggNOGiENOG410IFM8. Eukaryota.
ENOG410Y7VN. LUCA.
GeneTreeiENSGT00840000129674.
HOGENOMiHOG000231127.
HOVERGENiHBG009576.
InParanoidiP28230.
KOiK07620.
OMAiYLVVRAC.
OrthoDBiEOG7P2XSS.
PhylomeDBiP28230.
TreeFamiTF329606.

Enzyme and pathway databases

ReactomeiR-MMU-190704. Oligomerization of connexins into connexons.
R-MMU-190861. Gap junction assembly.

Miscellaneous databases

PMAP-CutDBP28230.
PROiP28230.
SOURCEiSearch...

Gene expression databases

BgeeiP28230.
CleanExiMM_GJB1.
GenevisibleiP28230. MM.

Family and domain databases

InterProiIPR000500. Connexin.
IPR002267. Connexin32.
IPR019570. Connexin_CCC.
IPR017990. Connexin_CS.
IPR013092. Connexin_N.
[Graphical view]
PANTHERiPTHR11984. PTHR11984. 1 hit.
PfamiPF00029. Connexin. 1 hit.
[Graphical view]
PRINTSiPR00206. CONNEXIN.
PR01138. CONNEXINB1.
SMARTiSM00037. CNX. 1 hit.
SM01089. Connexin_CCC. 1 hit.
[Graphical view]
PROSITEiPS00407. CONNEXINS_1. 1 hit.
PS00408. CONNEXINS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Developmental regulation of gap junction gene expression during mouse embryonic development."
    Nishi M., Kumar N.M., Gilula N.B.
    Dev. Biol. 146:117-130(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Molecular cloning of mouse connexins26 and -32: similar genomic organization but distinct promoter sequences of two gap junction genes."
    Willecke K., Nicholson B.J., Dahl E., Kozjek G., Hennemann H.
    Eur. J. Cell Biol. 58:81-89(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "A new alternatively spliced transcript of the mouse connexin32 gene is expressed in embryonic stem cells, oocytes and liver."
    Soehl G., Theis M., Hallas G., Brambach S., Dahl E., Kidder G., Willecke K.
    Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Liver.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Mammary tumor.
  5. "Connexins are critical for normal myelination in the CNS."
    Menichella D.M., Goodenough D.A., Sirkowski E., Scherer S.S., Paul D.L.
    J. Neurosci. 23:5963-5973(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  6. "Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis."
    Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H.
    J. Proteome Res. 7:3957-3967(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-258 AND SER-266, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  7. "Consortin, a trans-Golgi network cargo receptor for the plasma membrane targeting and recycling of connexins."
    del Castillo F.J., Cohen-Salmon M., Charollais A., Caille D., Lampe P.D., Chavrier P., Meda P., Petit C.
    Hum. Mol. Genet. 19:262-275(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CNST.

Entry informationi

Entry nameiCXB1_MOUSE
AccessioniPrimary (citable) accession number: P28230
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: May 10, 2004
Last modified: June 8, 2016
This is version 135 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.