P28229 (CXG1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gap junction gamma-1 protein

Alternative name(s):
Connexin-45
Short name=Cx45
Gap junction alpha-7 protein

Gene names

Name:	Gjc1
Synonyms:	Cxn-45, Gja7

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
Subunit structure	A connexon is composed of a hexamer of connexins. Interacts with CNST. Ref.4
Subcellular location	Cell membrane; Multi-pass membrane protein. Cell junction › gap junction.
Sequence similarities	Belongs to the connexin family. Gamma-type subfamily.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Gap junction Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cardiac muscle tissue development Inferred from mutant phenotype PubMed 14681043. Source: MGI cell development Inferred from mutant phenotype PubMed 14681043. Source: MGI synaptic transmission Inferred from mutant phenotype PubMed 15659592. Source: MGI vasculogenesis Inferred from mutant phenotype PubMed 10976050. Source: MGI visual perception Inferred from mutant phenotype PubMed 15659592. Source: MGI
Cellular_component	connexon complex Inferred from genetic interaction PubMed 15879306. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	gap junction channel activity Inferred from electronic annotation. Source: Compara ion channel activity Inferred from genetic interaction PubMed 15879306. Source: MGI
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Gja1	P23242	2	EBI-1767271,EBI-298630

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 396	396	Gap junction gamma-1 protein		PRO_0000057827
Regions
	Topological domain	1 – 22	22	Cytoplasmic Potential
	Transmembrane	23 – 45	23	Helical; Potential
	Topological domain	46 – 75	30	Extracellular Potential
	Transmembrane	76 – 95	20	Helical; Potential
	Topological domain	96 – 175	80	Cytoplasmic Potential
	Transmembrane	176 – 198	23	Helical; Potential
	Topological domain	199 – 228	30	Extracellular Potential
	Transmembrane	229 – 248	20	Helical; Potential
	Topological domain	249 – 396	148	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	P28229 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 9CD5721BB071B65C	FASTA	396	45,666
	10 20 30 40 50 60 MSWSFLTRLL EEIHNHSTFV GKIWLTVLIV FRIVLTAVGG ESIYYDEQSK FVCNTEQPGC 70 80 90 100 110 120 ENVCYDAFAP LSHVRFWVFQ IILVATPSVM YLGYAIHKIA KMEHGEADKK AARSKPYAMR 130 140 150 160 170 180 WKQHRALEET EEDHEEDPMM YPEMELESEK ENKEQSQPKP KHDGRRRIRE DGLMKIYVLQ 190 200 210 220 230 240 LLARTVFEVG FLIGQYFLYG FQVHPFYVCS RLPCPHKIDC FISRPTEKTI FLLIMYGVTG 250 260 270 280 290 300 LCLLLNIWEM LHLGFGTIRD SLNSKRRELD DPGAYNYPFT WNTPSAPPGY NIAVKPDQIQ 310 320 330 340 350 360 YTELSNAKIA YKQNKANIAQ EQQYGSHEEH LPADLETLQR EIRMAQERLD LAIQAYHHQN 370 380 390 NPHGPREKKA KVGSKSGSNK SSISSKSGDG KTSVWI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of gap junction genes expressed in F9 embryonic carcinoma cells: molecular cloning of mouse connexin31 and -45 cDNAs." Hennemann H., Schwarz H.J., Willecke K. Eur. J. Cell Biol. 57:51-58(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Sequence and structure of the mouse connexin45 gene." Baldridge D., Lecanda F., Shin C.S., Stains J., Civitelli R. Biosci. Rep. 21:683-689(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Embryo.
[4]	"Consortin, a trans-Golgi network cargo receptor for the plasma membrane targeting and recycling of connexins." del Castillo F.J., Cohen-Salmon M., Charollais A., Caille D., Lampe P.D., Chavrier P., Meda P., Petit C. Hum. Mol. Genet. 19:262-275(2010) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CNST.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X63100 mRNA. Translation: CAA44814.1. AJ300716 Genomic DNA. Translation: CAC21557.1. BC050840 mRNA. Translation: AAH50840.1. BC071230 mRNA. Translation: AAH71230.1.
IPI	IPI00118423.
PIR	S23589.
RefSeq	NP_001152854.1. NM_001159382.1. NP_001152855.1. NM_001159383.1. NP_032148.1. NM_008122.3.
UniGene	Mm.390369.
3D structure databases
ProteinModelPortal	P28229.
SMR	P28229. Positions 3-253.
ModBase	Search...
Protein-protein interaction databases
IntAct	P28229. 2 interactions.
MINT	MINT-210461.
STRING	10090.ENSMUSP00000090228.
PTM databases
PhosphoSite	P28229.
Proteomic databases
PRIDE	P28229.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000068933; ENSMUSP00000066901; ENSMUSG00000034520. ENSMUST00000092567; ENSMUSP00000090228; ENSMUSG00000034520. ENSMUST00000107075; ENSMUSP00000102690; ENSMUSG00000034520.
GeneID	14615.
KEGG	mmu:14615.
Organism-specific databases
CTD	10052.
MGI	MGI:95718. Gjc1.
Phylogenomic databases
eggNOG	NOG44555.
GeneTree	ENSGT00700000104468.
HOGENOM	HOG000231126.
HOVERGEN	HBG009576.
InParanoid	P28229.
KO	K07616.
OMA	KQHRGLE.
OrthoDB	EOG45HRXR.
Gene expression databases
ArrayExpress	P28229.
Bgee	P28229.
CleanEx	MM_GJC1.
Genevestigator	P28229.
GermOnline	ENSMUSG00000034520. Mus musculus.
Family and domain databases
InterPro	IPR000500. Connexin. IPR002265. Connexin45. IPR019570. Connexin_CCC. IPR017990. Connexin_CS. IPR013092. Connexin_N. [Graphical view]
PANTHER	PTHR11984. PTHR11984. 1 hit.
Pfam	PF00029. Connexin. 1 hit. PF10582. Connexin_CCC. 1 hit. [Graphical view]
PRINTS	PR00206. CONNEXIN. PR01136. CONNEXINA6.
SMART	SM00037. CNX. 1 hit. SM01089. Connexin_CCC. 1 hit. [Graphical view]
PROSITE	PS00407. CONNEXINS_1. 1 hit. PS00408. CONNEXINS_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	GJC1. mouse.
NextBio	286424.
SOURCE	Search...

Entry information

Entry name

CXG1_MOUSE

Accession

Primary (citable) accession number: P28229

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	May 1, 2013
This is version 114 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program