Reviewed, UniProtKB/Swiss-Prot P28227 (MAOX_ANAPL)

Names and origin

Protein names

Recommended name:
    NADP-dependent malic enzyme
      Short name=NADP-ME
    EC=1.1.1.40

Gene names

Name:

ME1

Organism

Anas platyrhynchos (Domestic duck) (Anas boschas)

Taxonomic identifier

8839 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Anseriformes › Anatidae › Anas

Protein attributes

Sequence length	557 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	(S)-malate + NADP(+) = pyruvate + CO(2) + NADPH.
Cofactor	Divalent metal cations. Prefers magnesium or manganese.
Subunit structure	Homotetramer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the malic enzymes family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 557	557	NADP-dependent malic enzyme		PRO_0000160196
Regions
	Nucleotide binding	290 – 307	18	NADP By similarity
Sites
	Active site	91	1	Proton donor By similarity
	Active site	162	1	Proton acceptor By similarity
	Metal binding	234	1	Divalent metal cation By similarity
	Metal binding	235	1	Divalent metal cation By similarity
	Metal binding	258	1	Divalent metal cation By similarity
	Binding site	144	1	NADP By similarity
	Binding site	258	1	NADP By similarity
	Binding site	397	1	NADP By similarity
	Site	258	1	Important for activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	P28227-1 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: BE6ED42C7017EF19	FASTA	557	61,898
	10 20 30 40 50 60 MKRGYEVLRD PHLNKGMAFT LEERQQLNIH GLLPPCFLGQ DVQVFSILKN FERLTSDLDR 70 80 90 100 110 120 YILLMSLQDR NEKLFYKVLT SDIERFMPIV YTPTVGLACQ QYGLAFRRPR GLFITIHDRG 130 140 150 160 170 180 HIATMLKSWP ESVIKAIVVT DGERILGLGD LGCYGMGIPV GKLALYTACG GVKPHECLPV 190 200 210 220 230 240 MLDVGTDNEA LLKDPLYIGL RHKRIRGQAY DDLLDEFMEA VTSRYGMNCL IQFEDFANAN 250 260 270 280 290 300 AFRLLHKYRN KYCTFNDDIQ GTASVAVAGL LAALRITKNR LSDHTVLFQG AGEAALGIAN 310 320 330 340 350 360 LIVMAMEKEG VSKEAAVKRI WMVDSKGLIV KGRASLTAEK TRFAHEHAEM KNLEDIVKDI 370 380 390 400 410 420 KPSVLIGVAA IGGAFTKEIL QDMAAFNKRP IIFALSNPTS KAECTAEQCY KYTEGRGIFA 430 440 450 460 470 480 SGSPFDPVTL PNGKTLYPGQ GNNSYVFPGV ALGVIACGLK HIGEDVFLTT AEVIAEQVSE 490 500 510 520 530 540 ENLQEGRLYP PLVTIQHVSL KIAVRIAEEA YRNNTASTYP QPKDLEAFIQ SQIYSTDYNS 550 FVADSYTWPE EAMKVKL

References

[1]

"Duck liver 'malic' enzyme. Expression in Escherichia coli and characterization of the wild-type enzyme and site-directed mutants." Hsu R.Y., Glynias M.J., Satterlee J.D., Feeney R., Clarke A.R., Emery D.S., Roe B.A., Wilson R.K., Goodridge A.G., Holbrook J.J. Biochem. J. 284:869-876(1992) [PubMed: 1622402] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, COFACTOR. Tissue: Liver.

[2]

"Duck liver malic enzyme: sequence of a tryptic peptide containing the cysteine residue labeled by the substrate analog bromopyruvate." Satterlee J.D., Hsu R.Y. Biochim. Biophys. Acta 1079:247-252(1991) [PubMed: 1911848] [Abstract] Cited for: PROTEIN SEQUENCE OF 86-107. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X66418 mRNA. Translation: CAA47049.1.
PIR	S23435.
3D structure databases
HSSP	HSSP built from PDB template 1GQ2 based on UniProtKB P40927.
ModBase	Search...
Phylogenomic databases
HOVERGEN	P28227.
Family and domain databases
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD_bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PRINTS	PR00072. MALOXRDTASE.
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAOX_ANAPL

Accession

Primary (citable) accession number: P28227

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	November 25, 2008
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)