Reviewed, UniProtKB/Swiss-Prot P28222 (5HT1B_HUMAN)

Names and origin

Protein names

Recommended name:
    5-hydroxytryptamine receptor 1B
Alternative name(s):
    5-HT-1D-beta
      Short name=5-HT-1B
      Short name=5-HT1B
    Serotonin receptor 1B
    Serotonin 1D beta receptor
    S12

Gene names

Name:	HTR1B
Synonyms:	HTR1DB

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	390 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Post-translational modification	Phosphorylated. Desensitization of the receptor may be mediated by its phosphorylation. Ref.12 Palmitoylated. Ref.12
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate Phosphoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	G-protein signaling, coupled to cyclic nucleotide second messenger Ref.4 Traceable author statement. Source: ProtInc synaptic transmission Ref.6 Traceable author statement. Source: ProtInc
Cellular component	integral to plasma membrane Ref.4 Traceable author statement. Source: ProtInc
Molecular function	protein binding Inferred from physical interaction. Source: IntAct serotonin receptor activity Ref.1 Traceable author statement. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GSTK1	Q9Y2Q3	1	EBI-1056863,EBI-1053767
NME2	P22392	1	EBI-1056863,EBI-713693

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 390	390	5-hydroxytryptamine receptor 1B		PRO_0000068916
Regions
	Topological domain	1 – 49	49	Extracellular By similarity
	Transmembrane	50 – 73	24	1 By similarity
	Topological domain	74 – 86	13	Cytoplasmic By similarity
	Transmembrane	87 – 109	23	2 By similarity
	Topological domain	110 – 119	10	Extracellular By similarity
	Transmembrane	120 – 145	26	3 By similarity
	Topological domain	146 – 165	20	Cytoplasmic By similarity
	Transmembrane	166 – 187	22	4 By similarity
	Topological domain	188 – 205	18	Extracellular By similarity
	Transmembrane	206 – 229	24	5 By similarity
	Topological domain	230 – 315	86	Cytoplasmic By similarity
	Transmembrane	316 – 340	25	6 By similarity
	Topological domain	341 – 347	7	Extracellular By similarity
	Transmembrane	348 – 373	26	7 By similarity
	Topological domain	374 – 390	17	Cytoplasmic By similarity
Amino acid modifications
	Lipidation	388	1	S-palmitoyl cysteine Potential
	Glycosylation	24	1	N-linked (GlcNAc...) Potential
	Glycosylation	32	1	N-linked (GlcNAc...) Potential
	Disulfide bond	122 ↔ 199		By similarity
Natural variations
	Natural variant	124	1	F → C: dbSNP rs130060. Ref.13		VAR_011715
	Natural variant	219	1	F → L: dbSNP rs130061.		VAR_011831
	Natural variant	367	1	I → V: dbSNP rs130063.		VAR_011832
	Natural variant	374	1	E → K: dbSNP rs130064.		VAR_011833

Sequences

	Sequence		Length	Mass (Da)
	P28222-1 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: CD874DC7EB44CF12	FASTA	390	43,568
	10 20 30 40 50 60 MEEPGAQCAP PPPAGSETWV PQANLSSAPS QNCSAKDYIY QDSISLPWKV LLVMLLALIT 70 80 90 100 110 120 LATTLSNAFV IATVYRTRKL HTPANYLIAS LAVTDLLVSI LVMPISTMYT VTGRWTLGQV 130 140 150 160 170 180 VCDFWLSSDI TCCTASILHL CVIALDRYWA ITDAVEYSAK RTPKRAAVMI ALVWVFSISI 190 200 210 220 230 240 SLPPFFWRQA KAEEEVSECV VNTDHILYTV YSTVGAFYFP TLLLIALYGR IYVEARSRIL 250 260 270 280 290 300 KQTPNRTGKR LTRAQLITDS PGSTSSVTSI NSRVPDVPSE SGSPVYVNQV KVRVSDALLE 310 320 330 340 350 360 KKKLMAARER KATKTLGIIL GAFIVCWLPF FIISLVMPIC KDACWFHLAI FDFFTWLGYL 370 380 390 NSLINPIIYT MSNEDFKQAF HKLIRFKCTS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and functional characterization of a human 5-HT1B serotonin receptor: a homologue of the rat 5-HT1B receptor with 5-HT1D-like pharmacological specificity." Hamblin M.W., Metcalf M.A., McGuffin R.W., Karpells S. Biochem. Biophys. Res. Commun. 184:752-759(1992) [PubMed: 1315531] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Cloning and expression of the human 5-HT1B-type receptor gene." Mochizuki D., Yuyama Y., Tsujita R., Komaki H., Sagai H. Biochem. Biophys. Res. Commun. 185:517-523(1992) [PubMed: 1610347] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Characterization of the human 5-hydroxytryptamine1B receptor." Jin H., Oksenberg D., Ashkenazi A., Peroutka S.J., Duncan A.M.V., Rozmahel R., Yang Y., Mengod G., Palacios J.M., O'Dowd B.F. J. Biol. Chem. 267:5735-5738(1992) [PubMed: 1348246] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Molecular cloning of a human serotonin receptor (S12) with a pharmacological profile resembling that of the 5-HT1D subtype." Levy F.O., Gudermann T., Perez-Reyes E., Birnbaumer M., Kaumann A.J., Birnbaumer L. J. Biol. Chem. 267:7553-7562(1992) [PubMed: 1559993] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"Human serotonin 1D receptor is encoded by a subfamily of two distinct genes: 5-HT1D alpha and 5-HT1D beta." Weinshank R.L., Zgombick J.M., Macchi M.J., Branchek T.A., Hartig P.R. Proc. Natl. Acad. Sci. U.S.A. 89:3630-3634(1992) [PubMed: 1565658] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Placenta.
[6]	"A human serotonin 1D receptor variant (5HT1D beta) encoded by an intronless gene on chromosome 6." Demchyshyn L., Sunahara R.K., Miller K., Teitler M., Hoffman B.J., Kennedy J.L., Seeman P., van Tol H.H.M., Niznik H.B. Proc. Natl. Acad. Sci. U.S.A. 89:5522-5526(1992) [PubMed: 1351684] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[7]	"Cloning and pharmacological characterization of a novel human 5-hydroxytryptamine1D receptor subtype." Veldman S.A., Bienkowski M.J. Mol. Pharmacol. 42:439-444(1992) [PubMed: 1328844] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[8]	"Human-specific amino acid changes found in 103 protein-coding genes." Kitano T., Liu Y.-H., Ueda S., Saitou N. Mol. Biol. Evol. 21:936-944(2004) [PubMed: 15014171] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[9]	"Isolation of complete coding sequence for 5-hydroxytryptamine (serotonin) receptor 1B (HTR1B)." Kopatz S.A., Aronstam R.S., Sharma S.V. Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[10]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed: 14574404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[11]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[12]	"Human serotonin1B receptor expression in Sf9 cells: phosphorylation, palmitoylation, and adenylyl cyclase inhibition." Ng G.Y.K., George S.R., Zastawny R.L., Caron M., Bouvier M., Dennis M., O'Dowd B.F. Biochemistry 32:11727-11733(1993) [PubMed: 8218242] [Abstract] Cited for: PALMITOYLATION, PHOSPHORYLATION.
[13]	"Identification of genetic variation in the human serotonin 1D beta receptor gene." Nothen M.M., Erdmann J., Shimron-Abarbanell D., Propping P. Biochem. Biophys. Res. Commun. 205:1194-1200(1994) [PubMed: 7802650] [Abstract] Cited for: VARIANT CYS-124.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M89478 Genomic DNA. No translation available.
D10995 Genomic DNA. Translation: BAA01763.1.
M83180 Genomic DNA. Translation: AAA36029.1.
L09732 Genomic DNA. Translation: AAA36030.1.
M81590 mRNA. Translation: AAA60316.1.
M75128 Genomic DNA. Translation: AAA58675.1.
AB041370 Genomic DNA. Translation: BAA94455.1.
AY225227 Genomic DNA. Translation: AAO67712.1.
AL049595 Genomic DNA. Translation: CAB51537.1.
BC069065 mRNA. Translation: AAH69065.1.
BC096206 mRNA. Translation: AAH96206.1.
BC096207 mRNA. Translation: AAH96207.1.
BC096208 mRNA. Translation: AAH96208.1.

IPI

IPI00002184.

PIR

JN0268.

RefSeq

NP_000854.1.

UniGene

Hs.123016

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2G1X	model	-	A	1-390	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P28222. 2 interactions.

Protein family/group databases

GPCRDB

Search...

Proteomic databases

PRIDE

P28222.

Genome annotation databases

Ensembl

ENSG00000135312. Homo sapiens. [Contig view]

GeneID

3351.

KEGG

hsa:3351.

UCSC

uc003pil.1. human.

Organism-specific databases

GeneCards

GC06M078228.

H-InvDB

HIX0032923.

HGNC

HGNC:5287. HTR1B.

MIM

182131. gene.

PharmGKB

PA29549.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

P28222.

HOVERGEN

P28222.

OMA

P28222. APSHNCS.

Enzyme and pathway databases

Reactome

REACT_14797. Signaling by GPCR.

Gene expression databases

ArrayExpress

P28222.

Bgee

P28222.

CleanEx

HS_HTR1B.

GermOnline

ENSG00000135312. Homo sapiens.

Family and domain databases

InterPro

IPR002147. 5HT1B_rcpt.
IPR002231. 5HT_rcpt.
IPR000276. 7TM_GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_supfam.
[Graphical view]

PANTHER

PTHR19266:SF67. 5HT1B_rcpt. 1 hit.

Pfam

PF00001. 7tm_1. 1 hit.
[Graphical view]

PRINTS

PR00513. 5HT1BRECEPTR.
PR01101. 5HTRECEPTOR.
PR00237. GPCRRHODOPSN.

PROSITE

PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

DrugBank

DB00918. Almotriptan.
DB01191. Dexfenfluramine.
DB00320. Dihydroergotamine.
DB00216. Eletriptan.
DB00696. Ergotamine.
DB00998. Frovatriptan.
DB00952. Naratriptan.
DB00960. Pindolol.
DB00571. Propranolol.
DB00953. Rizatriptan.
DB00669. Sumatriptan.
DB00285. Venlafaxine.
DB00315. Zolmitriptan.

NextBio

13252.

SOURCE

Search...

Entry information

Entry name

5HT1B_HUMAN

Accession

Primary (citable) accession number: P28222
Secondary accession number(s): Q4VAY7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	July 7, 2009
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)