P28188 (RAD2A_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ras-related protein RABD2a
Short name=AtRABD2a

Alternative name(s):
Ras-related protein Ara-5
Ras-related protein Rab1B
Short name=AtRab1B

Gene names

Name:	RABD2A
Synonyms:	ARA-5, RAB1B
Ordered Locus Names:	At1g02130
ORF Names:	T7I23.6

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	203 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Protein transport. Regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER). Ref.5 Ref.6
Subunit structure	Does not interact with GC5.
Subcellular location	Golgi apparatus › trans-Golgi network membrane. Golgi apparatus membrane; Lipid-anchor Probable Ref.9.
Sequence similarities	Belongs to the small GTPase superfamily. Rab family.
Sequence caution	The sequence AAC24370.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	ER-Golgi transport Protein transport Transport
Cellular component	Golgi apparatus Membrane
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Prenylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ER to Golgi vesicle-mediated transport Inferred from direct assay Ref.5. Source: TAIR protein transport Inferred from electronic annotation. Source: UniProtKB-KW response to cadmium ion Inferred from expression pattern. Source: TAIR small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	plasma membrane Inferred from direct assay. Source: TAIR trans-Golgi network membrane Inferred from direct assay Ref.9. Source: UniProtKB vacuole Inferred from direct assay. Source: TAIR
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 203	203	Ras-related protein RABD2a		PRO_0000121292
Regions
	Nucleotide binding	15 – 22	8	GTP By similarity
	Nucleotide binding	63 – 67	5	GTP By similarity
	Nucleotide binding	121 – 124	4	GTP By similarity
	Nucleotide binding	151 – 152	2	GTP By similarity
	Motif	37 – 45	9	Effector region By similarity
Amino acid modifications
	Lipidation	200	1	S-geranylgeranyl cysteine By similarity
	Lipidation	201	1	S-geranylgeranyl cysteine By similarity
Experimental info
	Mutagenesis	121	1	N → I: Inhibits trafficking between the ER and Golgi. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P28188 [UniParc]. Last modified September 2, 2008. Version 3. Checksum: 85A2870837300B8D	FASTA	203	22,648
	10 20 30 40 50 60 MNPEYDYLFK LLLIGDSGVG KSCLLLRFSD DSYVESYIST IGVDFKIRTV EQDGKTIKLQ 70 80 90 100 110 120 IWDTAGQERF RTITSSYYRG AHGIIIVYDV TDEESFNNVK QWLSEIDRYA SDNVNKLLVG 130 140 150 160 170 180 NKSDLTENRA IPYETAKAFA DEIGIPFMET SAKDATNVEQ AFMAMSASIK ERMASQPAGN 190 200 NARPPTVQIR GQPVAQKNGC CST

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Isolation and analysis of cDNAs encoding small GTP-binding proteins of Arabidopsis thaliana." Anai T., Hasegawa K., Watanabe Y., Uchimiya H., Ishizaki R., Matsui M. Gene 108:259-264(1991) [PubMed: 1748311] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 9-203. Strain: cv. Columbia, cv. En-1, cv. Est, cv. Landsberg erecta and cv. Lapalmam. Tissue: Leaf.
[5]	"A rab1 GTPase is required for transport between the endoplasmic reticulum and golgi apparatus and for normal golgi movement in plants." Batoko H., Zheng H.-Q., Hawes C., Moore I. Plant Cell 12:2201-2218(2000) [PubMed: 11090219] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF ASN-121.
[6]	"Redistribution of membrane proteins between the Golgi apparatus and endoplasmic reticulum in plants is reversible and not dependent on cytoskeletal networks." Saint-Jore C.M., Evins J., Batoko H., Brandizzi F., Moore I., Hawes C. Plant J. 29:661-678(2002) [PubMed: 11874578] [Abstract] Cited for: FUNCTION.
[7]	"Analysis of the small GTPase gene superfamily of Arabidopsis." Vernoud V., Horton A.C., Yang Z., Nielsen E. Plant Physiol. 131:1191-1208(2003) [PubMed: 12644670] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[8]	"Localization and domain characterization of Arabidopsis golgin candidates." Latijnhouwers M., Gillespie T., Boevink P., Kriechbaumer V., Hawes C., Carvalho C.M. J. Exp. Bot. 58:4373-4386(2007) [PubMed: 18182439] [Abstract] Cited for: INTERACTION WITH GC5.
[9]	"Genetic evidence that the higher plant Rab-D1 and Rab-D2 GTPases exhibit distinct but overlapping interactions in the early secretory pathway." Pinheiro H., Samalova M., Geldner N., Chory J., Martinez A., Moore I. J. Cell Sci. 122:3749-3758(2009) [PubMed: 19789181] [Abstract] Cited for: SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U89959 Genomic DNA. Translation: AAC24370.1. Sequence problems. CP002684 Genomic DNA. Translation: AEE27387.1. AY052333 mRNA. Translation: AAK96526.1. AY061905 mRNA. Translation: AAL31232.1. D01027 mRNA. Translation: BAA00832.1.
IPI	IPI00547166.
PIR	B86153. PS0279.
RefSeq	NP_171715.1. NM_100093.3.
UniGene	At.21490.
3D structure databases
ProteinModelPortal	P28188.
SMR	P28188. Positions 1-173.
ModBase	Search...
Protein-protein interaction databases
STRING	P28188.
Proteomic databases
PRIDE	P28188.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G02130.1; AT1G02130.1; AT1G02130.
GeneID	837023.
GenomeReviews	Gene locus AT1G02130 in contig CT485782_GR.
KEGG	ath:AT1G02130.
Organism-specific databases
TAIR	At1g02130.
Phylogenomic databases
eggNOG	KOG0084.
GeneTree	EPGT00070000028092.
HOGENOM	HBG745225.
InParanoid	P28188.
OMA	VESYIST.
PhylomeDB	P28188.
ProtClustDB	CLSN2914624.
Gene expression databases
ArrayExpress	P28188.
Genevestigator	P28188.
Family and domain databases
InterPro	IPR005225. Small_GTP-bd_dom. IPR001806. Small_GTPase. IPR003579. Small_GTPase_Rab_type. [Graphical view]
KO	K07976.
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
SMART	SM00175. RAB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00231. Small_GTP. 1 hit.
PROSITE	PS51419. RAB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RAD2A_ARATH

Accession

Primary (citable) accession number: P28188
Secondary accession number(s): Q940Z7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	September 2, 2008
Last modified:	November 16, 2011
This is version 95 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program