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Protein

Ras-related protein RABD2a

Gene

RABD2A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein transport. Regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER).2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi15 – 228GTPBy similarity
Nucleotide bindingi63 – 675GTPBy similarity
Nucleotide bindingi121 – 1244GTPBy similarity
Nucleotide bindingi151 – 1522GTPBy similarity

GO - Molecular functioni

  1. GDP binding Source: GO_Central
  2. GTPase activity Source: GO_Central
  3. GTP binding Source: GO_Central

GO - Biological processi

  1. ER to Golgi vesicle-mediated transport Source: TAIR
  2. intracellular protein transport Source: GO_Central
  3. metabolic process Source: GOC
  4. Rab protein signal transduction Source: GO_Central
  5. response to cadmium ion Source: TAIR
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein RABD2a
Short name:
AtRABD2a
Alternative name(s):
Ras-related protein Ara-5
Ras-related protein Rab1B
Short name:
AtRab1B
Gene namesi
Name:RABD2A
Synonyms:ARA-5, RAB1B
Ordered Locus Names:At1g02130
ORF Names:T7I23.6
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G02130.

Subcellular locationi

Golgi apparatustrans-Golgi network membrane 1 Publication. Golgi apparatus membrane 1 Publication; Lipid-anchor 1 Publication

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: GO_Central
  2. Golgi membrane Source: UniProtKB
  3. plasma membrane Source: TAIR
  4. trans-Golgi network membrane Source: UniProtKB
  5. vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi121 – 1211N → I: Inhibits trafficking between the ER and Golgi. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 203202Ras-related protein RABD2aPRO_0000121292Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi200 – 2001S-geranylgeranyl cysteineBy similarity
Lipidationi201 – 2011S-geranylgeranyl cysteineBy similarity

Keywords - PTMi

Lipoprotein, Prenylation

Proteomic databases

PaxDbiP28188.
PRIDEiP28188.

Expressioni

Gene expression databases

GenevestigatoriP28188.

Interactioni

Subunit structurei

Does not interact with GC5.

Protein-protein interaction databases

BioGridi22265. 1 interaction.
IntActiP28188. 1 interaction.
STRINGi3702.AT1G02130.1-P.

Structurei

3D structure databases

SMRiP28188. Positions 3-173.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi37 – 459Effector regionBy similarity

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiCOG1100.
HOGENOMiHOG000233968.
InParanoidiP28188.
KOiK07874.
OMAiKERMASQ.
PhylomeDBiP28188.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28188-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNPEYDYLFK LLLIGDSGVG KSCLLLRFSD DSYVESYIST IGVDFKIRTV
60 70 80 90 100
EQDGKTIKLQ IWDTAGQERF RTITSSYYRG AHGIIIVYDV TDEESFNNVK
110 120 130 140 150
QWLSEIDRYA SDNVNKLLVG NKSDLTENRA IPYETAKAFA DEIGIPFMET
160 170 180 190 200
SAKDATNVEQ AFMAMSASIK ERMASQPAGN NARPPTVQIR GQPVAQKNGC

CST
Length:203
Mass (Da):22,648
Last modified:September 1, 2008 - v3
Checksum:i85A2870837300B8D
GO

Sequence cautioni

The sequence AAC24370.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U89959 Genomic DNA. Translation: AAC24370.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE27387.1.
AY052333 mRNA. Translation: AAK96526.1.
AY061905 mRNA. Translation: AAL31232.1.
D01027 mRNA. Translation: BAA00832.1.
PIRiB86153.
PS0279.
RefSeqiNP_171715.1. NM_100093.3.
UniGeneiAt.21490.

Genome annotation databases

EnsemblPlantsiAT1G02130.1; AT1G02130.1; AT1G02130.
GeneIDi837023.
KEGGiath:AT1G02130.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U89959 Genomic DNA. Translation: AAC24370.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE27387.1.
AY052333 mRNA. Translation: AAK96526.1.
AY061905 mRNA. Translation: AAL31232.1.
D01027 mRNA. Translation: BAA00832.1.
PIRiB86153.
PS0279.
RefSeqiNP_171715.1. NM_100093.3.
UniGeneiAt.21490.

3D structure databases

SMRiP28188. Positions 3-173.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi22265. 1 interaction.
IntActiP28188. 1 interaction.
STRINGi3702.AT1G02130.1-P.

Proteomic databases

PaxDbiP28188.
PRIDEiP28188.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G02130.1; AT1G02130.1; AT1G02130.
GeneIDi837023.
KEGGiath:AT1G02130.

Organism-specific databases

TAIRiAT1G02130.

Phylogenomic databases

eggNOGiCOG1100.
HOGENOMiHOG000233968.
InParanoidiP28188.
KOiK07874.
OMAiKERMASQ.
PhylomeDBiP28188.

Gene expression databases

GenevestigatoriP28188.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Isolation and analysis of cDNAs encoding small GTP-binding proteins of Arabidopsis thaliana."
    Anai T., Hasegawa K., Watanabe Y., Uchimiya H., Ishizaki R., Matsui M.
    Gene 108:259-264(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 9-203.
    Strain: cv. Columbia, cv. En-1, cv. Est, cv. Landsberg erecta and cv. Lapalmam.
    Tissue: Leaf.
  5. "A rab1 GTPase is required for transport between the endoplasmic reticulum and Golgi apparatus and for normal Golgi movement in plants."
    Batoko H., Zheng H.-Q., Hawes C., Moore I.
    Plant Cell 12:2201-2218(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF ASN-121.
  6. "Redistribution of membrane proteins between the Golgi apparatus and endoplasmic reticulum in plants is reversible and not dependent on cytoskeletal networks."
    Saint-Jore C.M., Evins J., Batoko H., Brandizzi F., Moore I., Hawes C.
    Plant J. 29:661-678(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Analysis of the small GTPase gene superfamily of Arabidopsis."
    Vernoud V., Horton A.C., Yang Z., Nielsen E.
    Plant Physiol. 131:1191-1208(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  8. "Localization and domain characterization of Arabidopsis golgin candidates."
    Latijnhouwers M., Gillespie T., Boevink P., Kriechbaumer V., Hawes C., Carvalho C.M.
    J. Exp. Bot. 58:4373-4386(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GC5.
  9. "Genetic evidence that the higher plant Rab-D1 and Rab-D2 GTPases exhibit distinct but overlapping interactions in the early secretory pathway."
    Pinheiro H., Samalova M., Geldner N., Chory J., Martinez A., Moore I.
    J. Cell Sci. 122:3749-3758(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiRAD2A_ARATH
AccessioniPrimary (citable) accession number: P28188
Secondary accession number(s): Q940Z7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 30, 1992
Last sequence update: September 1, 2008
Last modified: March 31, 2015
This is version 120 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.