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P28184

- MT3_MOUSE

UniProt

P28184 - MT3_MOUSE

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Protein

Metallothionein-3

Gene

Mt3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Binds heavy metals. Contains three zinc and three copper atoms per polypeptide chain and only a negligible amount of cadmium. Inhibits survival and neurite formation of cortical neurons in vitro (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi6 – 61Divalent metal cation; cluster BBy similarity
Metal bindingi8 – 81Divalent metal cation; cluster BBy similarity
Metal bindingi14 – 141Divalent metal cation; cluster BBy similarity
Metal bindingi16 – 161Divalent metal cation; cluster BBy similarity
Metal bindingi20 – 201Divalent metal cation; cluster BBy similarity
Metal bindingi22 – 221Divalent metal cation; cluster BBy similarity
Metal bindingi25 – 251Divalent metal cation; cluster BBy similarity
Metal bindingi27 – 271Divalent metal cation; cluster BBy similarity
Metal bindingi30 – 301Divalent metal cation; cluster BBy similarity
Metal bindingi34 – 341Divalent metal cation; cluster ABy similarity
Metal bindingi35 – 351Divalent metal cation; cluster ABy similarity
Metal bindingi37 – 371Divalent metal cation; cluster ABy similarity
Metal bindingi38 – 381Divalent metal cation; cluster ABy similarity
Metal bindingi42 – 421Divalent metal cation; cluster ABy similarity
Metal bindingi45 – 451Divalent metal cation; cluster ABy similarity
Metal bindingi49 – 491Divalent metal cation; cluster ABy similarity
Metal bindingi51 – 511Divalent metal cation; cluster ABy similarity
Metal bindingi64 – 641Divalent metal cation; cluster ABy similarity
Metal bindingi66 – 661Divalent metal cation; cluster ABy similarity
Metal bindingi67 – 671Divalent metal cation; cluster ABy similarity

GO - Molecular functioni

  1. cadmium ion binding Source: UniProtKB
  2. copper ion binding Source: UniProtKB
  3. cysteine-type endopeptidase inhibitor activity involved in apoptotic process Source: UniProtKB
  4. drug binding Source: UniProtKB
  5. metal ion binding Source: MGI
  6. protein kinase activator activity Source: UniProtKB
  7. zinc ion binding Source: UniProtKB

GO - Biological processi

  1. activation of protein kinase B activity Source: UniProtKB
  2. astrocyte development Source: UniProtKB
  3. cadmium ion homeostasis Source: UniProtKB
  4. cellular lipid catabolic process Source: UniProtKB
  5. cellular metal ion homeostasis Source: MGI
  6. cellular response to cadmium ion Source: UniProtKB
  7. cellular response to drug Source: UniProtKB
  8. cellular response to hypoxia Source: Ensembl
  9. cellular response to nitric oxide Source: UniProtKB
  10. cellular response to oxidative stress Source: UniProtKB
  11. cellular zinc ion homeostasis Source: UniProtKB
  12. cholesterol catabolic process Source: UniProtKB
  13. energy reserve metabolic process Source: UniProtKB
  14. ERK1 and ERK2 cascade Source: UniProtKB
  15. histone modification Source: UniProtKB
  16. leptin-mediated signaling pathway Source: UniProtKB
  17. negative regulation of apoptotic process Source: UniProtKB
  18. negative regulation of autophagy Source: UniProtKB
  19. negative regulation of axon extension Source: UniProtKB
  20. negative regulation of cell growth Source: UniProtKB
  21. negative regulation of cysteine-type endopeptidase activity Source: UniProtKB
  22. negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
  23. negative regulation of necrotic cell death Source: UniProtKB
  24. negative regulation of neurogenesis Source: MGI
  25. negative regulation of neuron apoptotic process Source: UniProtKB
  26. negative regulation of oxidoreductase activity Source: UniProtKB
  27. negative regulation of reactive oxygen species metabolic process Source: UniProtKB
  28. negative regulation of transcription, DNA-templated Source: UniProtKB
  29. positive regulation of catalytic activity Source: UniProtKB
  30. positive regulation of cell death Source: UniProtKB
  31. positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
  32. positive regulation of gene expression Source: UniProtKB
  33. positive regulation of lysosomal membrane permeability Source: UniProtKB
  34. positive regulation of necrotic cell death Source: UniProtKB
  35. positive regulation of oxygen metabolic process Source: UniProtKB
  36. positive regulation of protein phosphorylation Source: UniProtKB
  37. positive regulation of transcription, DNA-templated Source: UniProtKB
  38. positive regulation of transcription from RNA polymerase II promoter in response to oxidative stress Source: UniProtKB
  39. positive regulation of vascular endothelial growth factor receptor signaling pathway Source: UniProtKB
  40. protein import into nucleus, translocation Source: UniProtKB
  41. protein kinase B signaling Source: UniProtKB
  42. protein stabilization Source: UniProtKB
  43. regulation of protein glycosylation Source: UniProtKB
  44. regulation of response to food Source: UniProtKB
  45. removal of superoxide radicals Source: UniProtKB
  46. response to hypoxia Source: UniProtKB
  47. response to oxidative stress Source: UniProtKB
  48. zinc ion homeostasis Source: UniProtKB
  49. zinc ion transport Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

Copper, Metal-binding, Metal-thiolate cluster, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Metallothionein-3
Short name:
MT-3
Alternative name(s):
Growth inhibitory factor
Short name:
GIF
Metallothionein-III
Short name:
MT-III
Gene namesi
Name:Mt3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 8

Organism-specific databases

MGIiMGI:97173. Mt3.

Subcellular locationi

GO - Cellular componenti

  1. inclusion body Source: UniProtKB
  2. intracellular Source: UniProtKB
  3. nucleus Source: UniProtKB
  4. perinuclear region of cytoplasm Source: UniProtKB
  5. synaptic vesicle Source: UniProtKB
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6868Metallothionein-3PRO_0000197251Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP28184.
PRIDEiP28184.

PTM databases

PhosphoSiteiP28184.

Expressioni

Tissue specificityi

Brain.

Gene expression databases

BgeeiP28184.
CleanExiMM_MT3.
ExpressionAtlasiP28184. baseline and differential.
GenevestigatoriP28184.

Structurei

Secondary structure

1
68
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi36 – 383Combined sources
Turni43 – 486Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1JI9NMR-A32-68[»]
ProteinModelPortaliP28184.
SMRiP28184. Positions 32-68.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP28184.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 3030BetaAdd
BLAST
Regioni31 – 6838AlphaAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000236262.
HOVERGENiHBG094960.
InParanoidiP28184.
KOiK14740.
OMAiTDSCKCE.

Family and domain databases

Gene3Di4.10.10.10. 1 hit.
InterProiIPR017854. Metalthion_dom.
IPR023587. Metalthion_dom_vert.
IPR003019. Metalthion_sfam_euk.
IPR000006. Metalthion_vert.
IPR018064. Metalthion_vert_metal_BS.
[Graphical view]
PANTHERiPTHR23299. PTHR23299. 1 hit.
PfamiPF00131. Metallothio. 1 hit.
[Graphical view]
PRINTSiPR00860. MTVERTEBRATE.
SUPFAMiSSF57868. SSF57868. 1 hit.
PROSITEiPS00203. METALLOTHIONEIN_VRT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P28184-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDPETCPCPT GGSCTCSDKC KCKGCKCTNC KKSCCSCCPA GCEKCAKDCV
60
CKGEEGAKAE AEKCSCCQ
Length:68
Mass (Da):7,009
Last modified:December 1, 1992 - v1
Checksum:i791AF60E38FED3CA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93310 Genomic DNA. Translation: AAA39529.1.
S72046 Genomic DNA. Translation: AAB31397.1.
BC059725 mRNA. Translation: AAH59725.1.
CCDSiCCDS40436.1.
PIRiA46034.
RefSeqiNP_038631.1. NM_013603.2.
UniGeneiMm.2064.

Genome annotation databases

EnsembliENSMUST00000034211; ENSMUSP00000034211; ENSMUSG00000031760.
GeneIDi17751.
KEGGimmu:17751.
UCSCiuc009mvu.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93310 Genomic DNA. Translation: AAA39529.1 .
S72046 Genomic DNA. Translation: AAB31397.1 .
BC059725 mRNA. Translation: AAH59725.1 .
CCDSi CCDS40436.1.
PIRi A46034.
RefSeqi NP_038631.1. NM_013603.2.
UniGenei Mm.2064.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1JI9 NMR - A 32-68 [» ]
ProteinModelPortali P28184.
SMRi P28184. Positions 32-68.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei P28184.

Proteomic databases

MaxQBi P28184.
PRIDEi P28184.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000034211 ; ENSMUSP00000034211 ; ENSMUSG00000031760 .
GeneIDi 17751.
KEGGi mmu:17751.
UCSCi uc009mvu.2. mouse.

Organism-specific databases

CTDi 4504.
MGIi MGI:97173. Mt3.

Phylogenomic databases

HOGENOMi HOG000236262.
HOVERGENi HBG094960.
InParanoidi P28184.
KOi K14740.
OMAi TDSCKCE.

Miscellaneous databases

EvolutionaryTracei P28184.
NextBioi 292415.
PROi P28184.
SOURCEi Search...

Gene expression databases

Bgeei P28184.
CleanExi MM_MT3.
ExpressionAtlasi P28184. baseline and differential.
Genevestigatori P28184.

Family and domain databases

Gene3Di 4.10.10.10. 1 hit.
InterProi IPR017854. Metalthion_dom.
IPR023587. Metalthion_dom_vert.
IPR003019. Metalthion_sfam_euk.
IPR000006. Metalthion_vert.
IPR018064. Metalthion_vert_metal_BS.
[Graphical view ]
PANTHERi PTHR23299. PTHR23299. 1 hit.
Pfami PF00131. Metallothio. 1 hit.
[Graphical view ]
PRINTSi PR00860. MTVERTEBRATE.
SUPFAMi SSF57868. SSF57868. 1 hit.
PROSITEi PS00203. METALLOTHIONEIN_VRT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "MT-III, a brain-specific member of the metallothionein gene family."
    Palmiter R.D., Findley S.D., Whitmore T.E., Durnam D.M.
    Proc. Natl. Acad. Sci. U.S.A. 89:6333-6337(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Brain.
  2. "Structures of the human and mouse growth inhibitory factor-encoding genes."
    Naruse S., Igarashi S., Furuya T., Kobayashi H., Miyatake T., Tsuji S.
    Gene 144:283-287(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  4. "Three-dimensional structure and dynamics of a brain specific growth inhibitory factor: metallothionein-3."
    Oz G., Zangger K., Armitage I.M.
    Biochemistry 40:11433-11441(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 32-68.

Entry informationi

Entry nameiMT3_MOUSE
AccessioniPrimary (citable) accession number: P28184
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: November 26, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Metallothioneins
    Classification of metallothioneins and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3