Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Metallothionein-3

Gene

Mt3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds heavy metals. Contains three zinc and three copper atoms per polypeptide chain and only a negligible amount of cadmium. Inhibits survival and neurite formation of cortical neurons in vitro (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi6Divalent metal cation; cluster BBy similarity1
Metal bindingi8Divalent metal cation; cluster BBy similarity1
Metal bindingi14Divalent metal cation; cluster BBy similarity1
Metal bindingi16Divalent metal cation; cluster BBy similarity1
Metal bindingi20Divalent metal cation; cluster BBy similarity1
Metal bindingi22Divalent metal cation; cluster BBy similarity1
Metal bindingi25Divalent metal cation; cluster BBy similarity1
Metal bindingi27Divalent metal cation; cluster BBy similarity1
Metal bindingi30Divalent metal cation; cluster BBy similarity1
Metal bindingi34Divalent metal cation; cluster ABy similarity1
Metal bindingi35Divalent metal cation; cluster ABy similarity1
Metal bindingi37Divalent metal cation; cluster ABy similarity1
Metal bindingi38Divalent metal cation; cluster ABy similarity1
Metal bindingi42Divalent metal cation; cluster ABy similarity1
Metal bindingi45Divalent metal cation; cluster ABy similarity1
Metal bindingi49Divalent metal cation; cluster ABy similarity1
Metal bindingi51Divalent metal cation; cluster ABy similarity1
Metal bindingi64Divalent metal cation; cluster ABy similarity1
Metal bindingi66Divalent metal cation; cluster ABy similarity1
Metal bindingi67Divalent metal cation; cluster ABy similarity1

GO - Molecular functioni

GO - Biological processi

  • activation of protein kinase B activity Source: UniProtKB
  • astrocyte development Source: UniProtKB
  • brain development Source: Ensembl
  • cadmium ion homeostasis Source: UniProtKB
  • cellular lipid catabolic process Source: UniProtKB
  • cellular metal ion homeostasis Source: MGI
  • cellular response to cadmium ion Source: UniProtKB
  • cellular response to drug Source: UniProtKB
  • cellular response to hypoxia Source: Ensembl
  • cellular response to nitric oxide Source: UniProtKB
  • cellular response to oxidative stress Source: UniProtKB
  • cellular zinc ion homeostasis Source: UniProtKB
  • cholesterol catabolic process Source: UniProtKB
  • energy reserve metabolic process Source: UniProtKB
  • ERK1 and ERK2 cascade Source: UniProtKB
  • histone modification Source: UniProtKB
  • leptin-mediated signaling pathway Source: UniProtKB
  • negative regulation of apoptotic process Source: UniProtKB
  • negative regulation of autophagy Source: UniProtKB
  • negative regulation of axon extension Source: UniProtKB
  • negative regulation of cell growth Source: UniProtKB
  • negative regulation of cysteine-type endopeptidase activity Source: UniProtKB
  • negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
  • negative regulation of hydrogen peroxide catabolic process Source: Ensembl
  • negative regulation of necrotic cell death Source: UniProtKB
  • negative regulation of neurogenesis Source: MGI
  • negative regulation of neuron apoptotic process Source: UniProtKB
  • negative regulation of oxidoreductase activity Source: UniProtKB
  • negative regulation of reactive oxygen species metabolic process Source: UniProtKB
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of catalytic activity Source: UniProtKB
  • positive regulation of cell death Source: UniProtKB
  • positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
  • positive regulation of gene expression Source: UniProtKB
  • positive regulation of lysosomal membrane permeability Source: UniProtKB
  • positive regulation of necrotic cell death Source: UniProtKB
  • positive regulation of oxygen metabolic process Source: UniProtKB
  • positive regulation of protein phosphorylation Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter in response to oxidative stress Source: UniProtKB
  • positive regulation of vascular endothelial growth factor receptor signaling pathway Source: UniProtKB
  • protein import into nucleus, translocation Source: UniProtKB
  • protein kinase B signaling Source: UniProtKB
  • protein stabilization Source: UniProtKB
  • regulation of protein glycosylation Source: UniProtKB
  • regulation of response to food Source: UniProtKB
  • removal of superoxide radicals Source: UniProtKB
  • response to hypoxia Source: UniProtKB
  • response to metal ion Source: Reactome
  • response to oxidative stress Source: UniProtKB
  • zinc II ion transport Source: UniProtKB
  • zinc ion homeostasis Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

Copper, Metal-binding, Metal-thiolate cluster, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-5661231. Metallothioneins bind metals.

Names & Taxonomyi

Protein namesi
Recommended name:
Metallothionein-3
Short name:
MT-3
Alternative name(s):
Growth inhibitory factor
Short name:
GIF
Metallothionein-III
Short name:
MT-III
Gene namesi
Name:Mt3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:97173. Mt3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001972511 – 68Metallothionein-3Add BLAST68

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei33PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP28184.
PaxDbiP28184.
PeptideAtlasiP28184.
PRIDEiP28184.

PTM databases

iPTMnetiP28184.
PhosphoSitePlusiP28184.

Expressioni

Tissue specificityi

Brain.

Gene expression databases

BgeeiENSMUSG00000031760.
CleanExiMM_MT3.
ExpressionAtlasiP28184. baseline and differential.
GenevisibleiP28184. MM.

Interactioni

Protein-protein interaction databases

BioGridi201581. 5 interactors.

Structurei

Secondary structure

168
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi36 – 38Combined sources3
Turni43 – 48Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JI9NMR-A32-68[»]
ProteinModelPortaliP28184.
SMRiP28184.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP28184.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 30BetaAdd BLAST30
Regioni31 – 68AlphaAdd BLAST38

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00730000111484.
HOGENOMiHOG000236262.
HOVERGENiHBG094960.
InParanoidiP28184.
KOiK14740.
OMAiTCPCPTG.

Family and domain databases

Gene3Di4.10.10.10. 1 hit.
InterProiIPR003019. Metalthion.
IPR017854. Metalthion_dom.
IPR023587. Metalthion_dom_vert.
IPR000006. Metalthion_vert.
IPR018064. Metalthion_vert_metal_BS.
[Graphical view]
PANTHERiPTHR23299. PTHR23299. 1 hit.
PfamiPF00131. Metallothio. 1 hit.
[Graphical view]
PRINTSiPR00860. MTVERTEBRATE.
SUPFAMiSSF57868. SSF57868. 1 hit.
PROSITEiPS00203. METALLOTHIONEIN_VRT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P28184-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPETCPCPT GGSCTCSDKC KCKGCKCTNC KKSCCSCCPA GCEKCAKDCV
60
CKGEEGAKAE AEKCSCCQ
Length:68
Mass (Da):7,009
Last modified:December 1, 1992 - v1
Checksum:i791AF60E38FED3CA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93310 Genomic DNA. Translation: AAA39529.1.
S72046 Genomic DNA. Translation: AAB31397.1.
BC059725 mRNA. Translation: AAH59725.1.
CCDSiCCDS40436.1.
PIRiA46034.
RefSeqiNP_038631.1. NM_013603.2.
UniGeneiMm.2064.

Genome annotation databases

EnsembliENSMUST00000034211; ENSMUSP00000034211; ENSMUSG00000031760.
GeneIDi17751.
KEGGimmu:17751.
UCSCiuc009mvu.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93310 Genomic DNA. Translation: AAA39529.1.
S72046 Genomic DNA. Translation: AAB31397.1.
BC059725 mRNA. Translation: AAH59725.1.
CCDSiCCDS40436.1.
PIRiA46034.
RefSeqiNP_038631.1. NM_013603.2.
UniGeneiMm.2064.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JI9NMR-A32-68[»]
ProteinModelPortaliP28184.
SMRiP28184.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201581. 5 interactors.

PTM databases

iPTMnetiP28184.
PhosphoSitePlusiP28184.

Proteomic databases

EPDiP28184.
PaxDbiP28184.
PeptideAtlasiP28184.
PRIDEiP28184.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034211; ENSMUSP00000034211; ENSMUSG00000031760.
GeneIDi17751.
KEGGimmu:17751.
UCSCiuc009mvu.2. mouse.

Organism-specific databases

CTDi4504.
MGIiMGI:97173. Mt3.

Phylogenomic databases

GeneTreeiENSGT00730000111484.
HOGENOMiHOG000236262.
HOVERGENiHBG094960.
InParanoidiP28184.
KOiK14740.
OMAiTCPCPTG.

Enzyme and pathway databases

ReactomeiR-MMU-5661231. Metallothioneins bind metals.

Miscellaneous databases

EvolutionaryTraceiP28184.
PROiP28184.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031760.
CleanExiMM_MT3.
ExpressionAtlasiP28184. baseline and differential.
GenevisibleiP28184. MM.

Family and domain databases

Gene3Di4.10.10.10. 1 hit.
InterProiIPR003019. Metalthion.
IPR017854. Metalthion_dom.
IPR023587. Metalthion_dom_vert.
IPR000006. Metalthion_vert.
IPR018064. Metalthion_vert_metal_BS.
[Graphical view]
PANTHERiPTHR23299. PTHR23299. 1 hit.
PfamiPF00131. Metallothio. 1 hit.
[Graphical view]
PRINTSiPR00860. MTVERTEBRATE.
SUPFAMiSSF57868. SSF57868. 1 hit.
PROSITEiPS00203. METALLOTHIONEIN_VRT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMT3_MOUSE
AccessioniPrimary (citable) accession number: P28184
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: November 2, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Metallothioneins
    Classification of metallothioneins and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.