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P28184

- MT3_MOUSE

UniProt

P28184 - MT3_MOUSE

Protein

Metallothionein-3

Gene

Mt3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Binds heavy metals. Contains three zinc and three copper atoms per polypeptide chain and only a negligible amount of cadmium. Inhibits survival and neurite formation of cortical neurons in vitro By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi6 – 61Divalent metal cation; cluster BBy similarity
    Metal bindingi8 – 81Divalent metal cation; cluster BBy similarity
    Metal bindingi14 – 141Divalent metal cation; cluster BBy similarity
    Metal bindingi16 – 161Divalent metal cation; cluster BBy similarity
    Metal bindingi20 – 201Divalent metal cation; cluster BBy similarity
    Metal bindingi22 – 221Divalent metal cation; cluster BBy similarity
    Metal bindingi25 – 251Divalent metal cation; cluster BBy similarity
    Metal bindingi27 – 271Divalent metal cation; cluster BBy similarity
    Metal bindingi30 – 301Divalent metal cation; cluster BBy similarity
    Metal bindingi34 – 341Divalent metal cation; cluster ABy similarity
    Metal bindingi35 – 351Divalent metal cation; cluster ABy similarity
    Metal bindingi37 – 371Divalent metal cation; cluster ABy similarity
    Metal bindingi38 – 381Divalent metal cation; cluster ABy similarity
    Metal bindingi42 – 421Divalent metal cation; cluster ABy similarity
    Metal bindingi45 – 451Divalent metal cation; cluster ABy similarity
    Metal bindingi49 – 491Divalent metal cation; cluster ABy similarity
    Metal bindingi51 – 511Divalent metal cation; cluster ABy similarity
    Metal bindingi64 – 641Divalent metal cation; cluster ABy similarity
    Metal bindingi66 – 661Divalent metal cation; cluster ABy similarity
    Metal bindingi67 – 671Divalent metal cation; cluster ABy similarity

    GO - Molecular functioni

    1. cadmium ion binding Source: UniProtKB
    2. copper ion binding Source: UniProtKB
    3. cysteine-type endopeptidase inhibitor activity involved in apoptotic process Source: UniProtKB
    4. drug binding Source: UniProtKB
    5. metal ion binding Source: MGI
    6. protein kinase activator activity Source: UniProtKB
    7. zinc ion binding Source: UniProtKB

    GO - Biological processi

    1. activation of protein kinase B activity Source: UniProtKB
    2. astrocyte development Source: UniProtKB
    3. cadmium ion homeostasis Source: UniProtKB
    4. cellular lipid catabolic process Source: UniProtKB
    5. cellular metal ion homeostasis Source: MGI
    6. cellular response to cadmium ion Source: UniProtKB
    7. cellular response to drug Source: UniProtKB
    8. cellular response to hypoxia Source: Ensembl
    9. cellular response to nitric oxide Source: UniProtKB
    10. cellular response to oxidative stress Source: UniProtKB
    11. cellular zinc ion homeostasis Source: UniProtKB
    12. cholesterol catabolic process Source: UniProtKB
    13. energy reserve metabolic process Source: UniProtKB
    14. ERK1 and ERK2 cascade Source: UniProtKB
    15. histone modification Source: UniProtKB
    16. leptin-mediated signaling pathway Source: UniProtKB
    17. negative regulation of apoptotic process Source: UniProtKB
    18. negative regulation of autophagy Source: UniProtKB
    19. negative regulation of axon extension Source: UniProtKB
    20. negative regulation of cell growth Source: UniProtKB
    21. negative regulation of cysteine-type endopeptidase activity Source: UniProtKB
    22. negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
    23. negative regulation of necrotic cell death Source: UniProtKB
    24. negative regulation of neurogenesis Source: MGI
    25. negative regulation of neuron apoptotic process Source: UniProtKB
    26. negative regulation of oxidoreductase activity Source: UniProtKB
    27. negative regulation of reactive oxygen species metabolic process Source: UniProtKB
    28. negative regulation of transcription, DNA-templated Source: UniProtKB
    29. positive regulation of catalytic activity Source: UniProtKB
    30. positive regulation of cell death Source: UniProtKB
    31. positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
    32. positive regulation of gene expression Source: UniProtKB
    33. positive regulation of lysosomal membrane permeability Source: UniProtKB
    34. positive regulation of necrotic cell death Source: UniProtKB
    35. positive regulation of oxygen metabolic process Source: UniProtKB
    36. positive regulation of protein phosphorylation Source: UniProtKB
    37. positive regulation of transcription, DNA-templated Source: UniProtKB
    38. positive regulation of transcription from RNA polymerase II promoter in response to oxidative stress Source: UniProtKB
    39. positive regulation of vascular endothelial growth factor receptor signaling pathway Source: UniProtKB
    40. protein import into nucleus, translocation Source: UniProtKB
    41. protein kinase B signaling Source: UniProtKB
    42. protein stabilization Source: UniProtKB
    43. regulation of protein glycosylation Source: UniProtKB
    44. regulation of response to food Source: UniProtKB
    45. removal of superoxide radicals Source: UniProtKB
    46. response to hypoxia Source: UniProtKB
    47. response to oxidative stress Source: UniProtKB
    48. zinc ion homeostasis Source: UniProtKB
    49. zinc ion transport Source: UniProtKB

    Keywords - Ligandi

    Copper, Metal-binding, Metal-thiolate cluster, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Metallothionein-3
    Short name:
    MT-3
    Alternative name(s):
    Growth inhibitory factor
    Short name:
    GIF
    Metallothionein-III
    Short name:
    MT-III
    Gene namesi
    Name:Mt3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 8

    Organism-specific databases

    MGIiMGI:97173. Mt3.

    Subcellular locationi

    GO - Cellular componenti

    1. inclusion body Source: UniProtKB
    2. intracellular Source: UniProtKB
    3. nucleus Source: UniProtKB
    4. perinuclear region of cytoplasm Source: UniProtKB
    5. synaptic vesicle Source: UniProtKB

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 6868Metallothionein-3PRO_0000197251Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PRIDEiP28184.

    PTM databases

    PhosphoSiteiP28184.

    Expressioni

    Tissue specificityi

    Brain.

    Gene expression databases

    BgeeiP28184.
    CleanExiMM_MT3.
    GenevestigatoriP28184.

    Structurei

    Secondary structure

    1
    68
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi36 – 383
    Turni43 – 486

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1JI9NMR-A32-68[»]
    ProteinModelPortaliP28184.
    SMRiP28184. Positions 32-68.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP28184.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 3030BetaAdd
    BLAST
    Regioni31 – 6838AlphaAdd
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    HOGENOMiHOG000236262.
    HOVERGENiHBG094960.
    InParanoidiP28184.
    KOiK14740.
    OMAiTDSCKCE.

    Family and domain databases

    Gene3Di4.10.10.10. 1 hit.
    InterProiIPR017854. Metalthion_dom.
    IPR023587. Metalthion_dom_vert.
    IPR003019. Metalthion_sfam_euk.
    IPR000006. Metalthion_vert.
    IPR018064. Metalthion_vert_metal_BS.
    [Graphical view]
    PANTHERiPTHR23299. PTHR23299. 1 hit.
    PfamiPF00131. Metallothio. 1 hit.
    [Graphical view]
    PRINTSiPR00860. MTVERTEBRATE.
    SUPFAMiSSF57868. SSF57868. 1 hit.
    PROSITEiPS00203. METALLOTHIONEIN_VRT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P28184-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDPETCPCPT GGSCTCSDKC KCKGCKCTNC KKSCCSCCPA GCEKCAKDCV   50
    CKGEEGAKAE AEKCSCCQ 68
    Length:68
    Mass (Da):7,009
    Last modified:December 1, 1992 - v1
    Checksum:i791AF60E38FED3CA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M93310 Genomic DNA. Translation: AAA39529.1.
    S72046 Genomic DNA. Translation: AAB31397.1.
    BC059725 mRNA. Translation: AAH59725.1.
    CCDSiCCDS40436.1.
    PIRiA46034.
    RefSeqiNP_038631.1. NM_013603.2.
    UniGeneiMm.2064.

    Genome annotation databases

    EnsembliENSMUST00000034211; ENSMUSP00000034211; ENSMUSG00000031760.
    GeneIDi17751.
    KEGGimmu:17751.
    UCSCiuc009mvu.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M93310 Genomic DNA. Translation: AAA39529.1 .
    S72046 Genomic DNA. Translation: AAB31397.1 .
    BC059725 mRNA. Translation: AAH59725.1 .
    CCDSi CCDS40436.1.
    PIRi A46034.
    RefSeqi NP_038631.1. NM_013603.2.
    UniGenei Mm.2064.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1JI9 NMR - A 32-68 [» ]
    ProteinModelPortali P28184.
    SMRi P28184. Positions 32-68.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei P28184.

    Proteomic databases

    PRIDEi P28184.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000034211 ; ENSMUSP00000034211 ; ENSMUSG00000031760 .
    GeneIDi 17751.
    KEGGi mmu:17751.
    UCSCi uc009mvu.2. mouse.

    Organism-specific databases

    CTDi 4504.
    MGIi MGI:97173. Mt3.

    Phylogenomic databases

    HOGENOMi HOG000236262.
    HOVERGENi HBG094960.
    InParanoidi P28184.
    KOi K14740.
    OMAi TDSCKCE.

    Miscellaneous databases

    EvolutionaryTracei P28184.
    NextBioi 292415.
    PROi P28184.
    SOURCEi Search...

    Gene expression databases

    Bgeei P28184.
    CleanExi MM_MT3.
    Genevestigatori P28184.

    Family and domain databases

    Gene3Di 4.10.10.10. 1 hit.
    InterProi IPR017854. Metalthion_dom.
    IPR023587. Metalthion_dom_vert.
    IPR003019. Metalthion_sfam_euk.
    IPR000006. Metalthion_vert.
    IPR018064. Metalthion_vert_metal_BS.
    [Graphical view ]
    PANTHERi PTHR23299. PTHR23299. 1 hit.
    Pfami PF00131. Metallothio. 1 hit.
    [Graphical view ]
    PRINTSi PR00860. MTVERTEBRATE.
    SUPFAMi SSF57868. SSF57868. 1 hit.
    PROSITEi PS00203. METALLOTHIONEIN_VRT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "MT-III, a brain-specific member of the metallothionein gene family."
      Palmiter R.D., Findley S.D., Whitmore T.E., Durnam D.M.
      Proc. Natl. Acad. Sci. U.S.A. 89:6333-6337(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Tissue: Brain.
    2. "Structures of the human and mouse growth inhibitory factor-encoding genes."
      Naruse S., Igarashi S., Furuya T., Kobayashi H., Miyatake T., Tsuji S.
      Gene 144:283-287(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    4. "Three-dimensional structure and dynamics of a brain specific growth inhibitory factor: metallothionein-3."
      Oz G., Zangger K., Armitage I.M.
      Biochemistry 40:11433-11441(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 32-68.

    Entry informationi

    Entry nameiMT3_MOUSE
    AccessioniPrimary (citable) accession number: P28184
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: December 1, 1992
    Last modified: October 1, 2014
    This is version 122 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Metallothioneins
      Classification of metallothioneins and list of entries
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3