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Protein

Metallothionein-3

Gene

Mt3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds heavy metals. Contains three zinc and three copper atoms per polypeptide chain and only a negligible amount of cadmium. Inhibits survival and neurite formation of cortical neurons in vitro (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi6Divalent metal cation; cluster BBy similarity1
Metal bindingi8Divalent metal cation; cluster BBy similarity1
Metal bindingi14Divalent metal cation; cluster BBy similarity1
Metal bindingi16Divalent metal cation; cluster BBy similarity1
Metal bindingi20Divalent metal cation; cluster BBy similarity1
Metal bindingi22Divalent metal cation; cluster BBy similarity1
Metal bindingi25Divalent metal cation; cluster BBy similarity1
Metal bindingi27Divalent metal cation; cluster BBy similarity1
Metal bindingi30Divalent metal cation; cluster BBy similarity1
Metal bindingi34Divalent metal cation; cluster ABy similarity1
Metal bindingi35Divalent metal cation; cluster ABy similarity1
Metal bindingi37Divalent metal cation; cluster ABy similarity1
Metal bindingi38Divalent metal cation; cluster ABy similarity1
Metal bindingi42Divalent metal cation; cluster ABy similarity1
Metal bindingi45Divalent metal cation; cluster ABy similarity1
Metal bindingi49Divalent metal cation; cluster ABy similarity1
Metal bindingi51Divalent metal cation; cluster ABy similarity1
Metal bindingi64Divalent metal cation; cluster ABy similarity1
Metal bindingi66Divalent metal cation; cluster ABy similarity1
Metal bindingi67Divalent metal cation; cluster ABy similarity1

GO - Molecular functioni

GO - Biological processi

  • activation of protein kinase B activity Source: UniProtKB
  • astrocyte development Source: UniProtKB
  • brain development Source: Ensembl
  • cadmium ion homeostasis Source: UniProtKB
  • cellular lipid catabolic process Source: UniProtKB
  • cellular metal ion homeostasis Source: MGI
  • cellular response to cadmium ion Source: UniProtKB
  • cellular response to drug Source: UniProtKB
  • cellular response to hypoxia Source: Ensembl
  • cellular response to nitric oxide Source: UniProtKB
  • cellular response to oxidative stress Source: UniProtKB
  • cellular zinc ion homeostasis Source: UniProtKB
  • cholesterol catabolic process Source: UniProtKB
  • energy reserve metabolic process Source: UniProtKB
  • ERK1 and ERK2 cascade Source: UniProtKB
  • histone modification Source: UniProtKB
  • leptin-mediated signaling pathway Source: UniProtKB
  • negative regulation of apoptotic process Source: UniProtKB
  • negative regulation of autophagy Source: UniProtKB
  • negative regulation of axon extension Source: UniProtKB
  • negative regulation of cell growth Source: UniProtKB
  • negative regulation of cysteine-type endopeptidase activity Source: UniProtKB
  • negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
  • negative regulation of hydrogen peroxide catabolic process Source: Ensembl
  • negative regulation of necrotic cell death Source: UniProtKB
  • negative regulation of neurogenesis Source: MGI
  • negative regulation of neuron apoptotic process Source: UniProtKB
  • negative regulation of oxidoreductase activity Source: UniProtKB
  • negative regulation of reactive oxygen species metabolic process Source: UniProtKB
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of catalytic activity Source: UniProtKB
  • positive regulation of cell death Source: UniProtKB
  • positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
  • positive regulation of gene expression Source: UniProtKB
  • positive regulation of lysosomal membrane permeability Source: UniProtKB
  • positive regulation of necrotic cell death Source: UniProtKB
  • positive regulation of oxygen metabolic process Source: UniProtKB
  • positive regulation of protein phosphorylation Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter in response to oxidative stress Source: UniProtKB
  • positive regulation of vascular endothelial growth factor receptor signaling pathway Source: UniProtKB
  • protein import into nucleus, translocation Source: UniProtKB
  • protein kinase B signaling Source: UniProtKB
  • protein stabilization Source: UniProtKB
  • regulation of protein glycosylation Source: UniProtKB
  • regulation of response to food Source: UniProtKB
  • removal of superoxide radicals Source: UniProtKB
  • response to hypoxia Source: UniProtKB
  • response to oxidative stress Source: UniProtKB
  • zinc II ion transport Source: UniProtKB
  • zinc ion homeostasis Source: UniProtKB

Keywordsi

LigandCopper, Metal-binding, Metal-thiolate cluster, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-5661231 Metallothioneins bind metals

Names & Taxonomyi

Protein namesi
Recommended name:
Metallothionein-3
Short name:
MT-3
Alternative name(s):
Growth inhibitory factor
Short name:
GIF
Metallothionein-III
Short name:
MT-III
Gene namesi
Name:Mt3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:97173 Mt3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001972511 – 68Metallothionein-3Add BLAST68

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei33PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP28184
PaxDbiP28184
PeptideAtlasiP28184
PRIDEiP28184

PTM databases

iPTMnetiP28184
PhosphoSitePlusiP28184

Expressioni

Tissue specificityi

Brain.

Gene expression databases

BgeeiENSMUSG00000031760
CleanExiMM_MT3
ExpressionAtlasiP28184 baseline and differential
GenevisibleiP28184 MM

Interactioni

Protein-protein interaction databases

BioGridi201581, 5 interactors
CORUMiP28184
STRINGi10090.ENSMUSP00000034211

Structurei

Secondary structure

168
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi36 – 38Combined sources3
Turni43 – 48Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JI9NMR-A32-68[»]
ProteinModelPortaliP28184
SMRiP28184
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP28184

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 30BetaAdd BLAST30
Regioni31 – 68AlphaAdd BLAST38

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00730000111484
HOGENOMiHOG000236262
HOVERGENiHBG094960
InParanoidiP28184
KOiK14740
OMAiVCKGEEG

Family and domain databases

Gene3Di4.10.10.10, 1 hit
InterProiView protein in InterPro
IPR003019 Metalthion
IPR017854 Metalthion_dom_sf
IPR023587 Metalthion_dom_sf_vert
IPR000006 Metalthion_vert
IPR018064 Metalthion_vert_metal_BS
PANTHERiPTHR23299 PTHR23299, 1 hit
PfamiView protein in Pfam
PF00131 Metallothio, 1 hit
PRINTSiPR00860 MTVERTEBRATE
SUPFAMiSSF57868 SSF57868, 1 hit
PROSITEiView protein in PROSITE
PS00203 METALLOTHIONEIN_VRT, 1 hit

Sequencei

Sequence statusi: Complete.

P28184-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPETCPCPT GGSCTCSDKC KCKGCKCTNC KKSCCSCCPA GCEKCAKDCV
60
CKGEEGAKAE AEKCSCCQ
Length:68
Mass (Da):7,009
Last modified:December 1, 1992 - v1
Checksum:i791AF60E38FED3CA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93310 Genomic DNA Translation: AAA39529.1
S72046 Genomic DNA Translation: AAB31397.1
BC059725 mRNA Translation: AAH59725.1
CCDSiCCDS40436.1
PIRiA46034
RefSeqiNP_038631.1, NM_013603.2
UniGeneiMm.2064

Genome annotation databases

EnsembliENSMUST00000034211; ENSMUSP00000034211; ENSMUSG00000031760
GeneIDi17751
KEGGimmu:17751
UCSCiuc009mvu.2 mouse

Similar proteinsi

Entry informationi

Entry nameiMT3_MOUSE
AccessioniPrimary (citable) accession number: P28184
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: March 28, 2018
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health