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P28173

- PUR1_CHICK

UniProt

P28173 - PUR1_CHICK

Protein

Amidophosphoribosyltransferase

Gene

PPAT

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 1 (01 Dec 1992)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity
    Binds 1 4Fe-4S cluster per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei12 – 121For GATase activityBy similarity
    Metal bindingi280 – 2801Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi327 – 3271MagnesiumBy similarity
    Metal bindingi389 – 3891MagnesiumBy similarity
    Metal bindingi390 – 3901MagnesiumBy similarity
    Metal bindingi426 – 4261Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi496 – 4961Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi499 – 4991Iron-sulfur (4Fe-4S)By similarity

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. amidophosphoribosyltransferase activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. 'de novo' IMP biosynthetic process Source: Reactome
    2. glutamine metabolic process Source: UniProtKB-KW
    3. nucleobase-containing small molecule metabolic process Source: Reactome
    4. nucleoside metabolic process Source: InterPro
    5. purine nucleobase biosynthetic process Source: InterPro
    6. purine nucleobase metabolic process Source: Reactome
    7. small molecule metabolic process Source: Reactome

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine biosynthesis

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Magnesium, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_116007. De novo synthesis of IMP.
    UniPathwayiUPA00074; UER00124.

    Protein family/group databases

    MEROPSiC44.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Amidophosphoribosyltransferase (EC:2.4.2.14)
    Short name:
    ATase
    Alternative name(s):
    Glutamine phosphoribosylpyrophosphate amidotransferase
    Short name:
    GPAT
    Gene namesi
    Name:PPAT
    Synonyms:GPAT
    OrganismiGallus gallus (Chicken)
    Taxonomic identifieri9031 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
    ProteomesiUP000000539: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: Reactome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei1 – 1111CuratedPRO_0000029281Add
    BLAST
    Chaini12 – 510499AmidophosphoribosyltransferasePRO_0000029282Add
    BLAST

    Proteomic databases

    PaxDbiP28173.
    PRIDEiP28173.

    Interactioni

    Subunit structurei

    Homotetramer.

    Structurei

    3D structure databases

    ProteinModelPortaliP28173.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini12 – 261250Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.Curated
    Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Glutamine amidotransferase

    Phylogenomic databases

    eggNOGiCOG0034.
    HOGENOMiHOG000033688.
    HOVERGENiHBG002589.
    InParanoidiP28173.
    KOiK00764.

    Family and domain databases

    Gene3Di3.40.50.2020. 1 hit.
    3.60.20.10. 1 hit.
    InterProiIPR005854. Amd_phspho_trans.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view]
    PfamiPF00310. GATase_2. 2 hits.
    PF00156. Pribosyltran. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
    SUPFAMiSSF53271. SSF53271. 1 hit.
    SSF56235. SSF56235. 1 hit.
    TIGRFAMsiTIGR01134. purF. 1 hit.
    PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
    PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P28173-1 [UniParc]FASTAAdd to Basket

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    MELEELGIRE ECGVFGCIAA GVWPTELDVP HVITLGLVGL QHRGQESAGI    50
    VTSDGESSQA FKVHKGMGLI NHVFNADSLK KLYVSNLGIG HTRYSTSGIS 100
    ELQNCQPFVV ETLHGKIAVA HNGELTNAVR LRRKLMRHGV GLSTSSDSEL 150
    ITQLLAFTPP LENDDTADWV ARIKNLMNET PTSYSLLIMH KDIIYAVRDP 200
    YGNRPLCIGR LIPVGDINGK GKDNSETEGW VVSSESCSFL SIGAEYYREV 250
    LPGEIVKISR YDVQTLDVVP RPEGDPSAFC IFEYVYFARP DSIFEGQMVY 300
    SVRRRCGQQL AIEAPVEADL VSTVPESATP AALGYAQKCG LPYVEVLCKN 350
    RYVGRTFIQP NMRLRQLGVA KKFGVLSDNF KGKRVVIIDD SIVRGNTISP 400
    IIKLLRESGA KEVHIRVASP PIRFPCYMGI NIPTKEELIA NRPEFHDLAN 450
    YIGADSVVYL SVEGLVSSVQ ESIKARKENE NSLKTQKSRV GKIGHCTACL 500
    TGDYPVELEW 510
    Length:510
    Mass (Da):56,257
    Last modified:December 1, 1992 - v1
    Checksum:iF4371FE1FEC7C744
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M60069 mRNA. Translation: AAA62736.1.
    L12533 Unassigned DNA. Translation: AAA17895.1.
    PIRiA38337.
    RefSeqiNP_001004401.1. NM_001004401.1.
    UniGeneiGga.2870.

    Genome annotation databases

    GeneIDi422743.
    KEGGigga:422743.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M60069 mRNA. Translation: AAA62736.1 .
    L12533 Unassigned DNA. Translation: AAA17895.1 .
    PIRi A38337.
    RefSeqi NP_001004401.1. NM_001004401.1.
    UniGenei Gga.2870.

    3D structure databases

    ProteinModelPortali P28173.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C44.001.

    Proteomic databases

    PaxDbi P28173.
    PRIDEi P28173.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 422743.
    KEGGi gga:422743.

    Organism-specific databases

    CTDi 5471.

    Phylogenomic databases

    eggNOGi COG0034.
    HOGENOMi HOG000033688.
    HOVERGENi HBG002589.
    InParanoidi P28173.
    KOi K00764.

    Enzyme and pathway databases

    UniPathwayi UPA00074 ; UER00124 .
    Reactomei REACT_116007. De novo synthesis of IMP.

    Miscellaneous databases

    NextBioi 20825333.
    PROi P28173.

    Family and domain databases

    Gene3Di 3.40.50.2020. 1 hit.
    3.60.20.10. 1 hit.
    InterProi IPR005854. Amd_phspho_trans.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view ]
    Pfami PF00310. GATase_2. 2 hits.
    PF00156. Pribosyltran. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000485. Amd_phspho_trans. 1 hit.
    SUPFAMi SSF53271. SSF53271. 1 hit.
    SSF56235. SSF56235. 1 hit.
    TIGRFAMsi TIGR01134. purF. 1 hit.
    PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
    PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of avian glutamine phosphoribosylpyrophosphate amidotransferase. Conservation of a bacterial propeptide sequence supports a role for posttranslational processing."
      Zhou G., Dixon J.E., Zalkin H.
      J. Biol. Chem. 265:21152-21159(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Coexpression of two closely linked avian genes for purine nucleotide synthesis from a bidirectional promoter."
      Gavalas A., Dixon J.E., Brayton K.A., Zalkin H.
      Mol. Cell. Biol. 13:4784-4792(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE OF 1-42.
    3. Erratum
      Gavalas A., Dixon J.E., Brayton K.A., Zalkin H.
      Mol. Cell. Biol. 13:7977-7977(1993)

    Entry informationi

    Entry nameiPUR1_CHICK
    AccessioniPrimary (citable) accession number: P28173
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: December 1, 1992
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3