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P28173

- PUR1_CHICK

UniProt

P28173 - PUR1_CHICK

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Protein
Amidophosphoribosyltransferase
Gene
PPAT, GPAT
Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

Cofactori

Binds 1 magnesium ion per subunit By similarity.
Binds 1 4Fe-4S cluster per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei12 – 121For GATase activity By similarity
Metal bindingi280 – 2801Iron-sulfur (4Fe-4S) By similarity
Metal bindingi327 – 3271Magnesium By similarity
Metal bindingi389 – 3891Magnesium By similarity
Metal bindingi390 – 3901Magnesium By similarity
Metal bindingi426 – 4261Iron-sulfur (4Fe-4S) By similarity
Metal bindingi496 – 4961Iron-sulfur (4Fe-4S) By similarity
Metal bindingi499 – 4991Iron-sulfur (4Fe-4S) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. amidophosphoribosyltransferase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: Reactome
  2. glutamine metabolic process Source: UniProtKB-KW
  3. nucleobase-containing small molecule metabolic process Source: Reactome
  4. nucleoside metabolic process Source: InterPro
  5. purine nucleobase biosynthetic process Source: InterPro
  6. purine nucleobase metabolic process Source: Reactome
  7. small molecule metabolic process Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Magnesium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_116007. De novo synthesis of IMP.
UniPathwayiUPA00074; UER00124.

Protein family/group databases

MEROPSiC44.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Amidophosphoribosyltransferase (EC:2.4.2.14)
Short name:
ATase
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase
Short name:
GPAT
Gene namesi
Name:PPAT
Synonyms:GPAT
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: Reactome
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 1111 Inferred
PRO_0000029281Add
BLAST
Chaini12 – 510499Amidophosphoribosyltransferase
PRO_0000029282Add
BLAST

Proteomic databases

PaxDbiP28173.
PRIDEiP28173.

Interactioni

Subunit structurei

Homotetramer.

Structurei

3D structure databases

ProteinModelPortaliP28173.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini12 – 261250Glutamine amidotransferase type-2
Add
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0034.
HOGENOMiHOG000033688.
HOVERGENiHBG002589.
InParanoidiP28173.
KOiK00764.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00310. GATase_2. 2 hits.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28173-1 [UniParc]FASTAAdd to Basket

« Hide

MELEELGIRE ECGVFGCIAA GVWPTELDVP HVITLGLVGL QHRGQESAGI    50
VTSDGESSQA FKVHKGMGLI NHVFNADSLK KLYVSNLGIG HTRYSTSGIS 100
ELQNCQPFVV ETLHGKIAVA HNGELTNAVR LRRKLMRHGV GLSTSSDSEL 150
ITQLLAFTPP LENDDTADWV ARIKNLMNET PTSYSLLIMH KDIIYAVRDP 200
YGNRPLCIGR LIPVGDINGK GKDNSETEGW VVSSESCSFL SIGAEYYREV 250
LPGEIVKISR YDVQTLDVVP RPEGDPSAFC IFEYVYFARP DSIFEGQMVY 300
SVRRRCGQQL AIEAPVEADL VSTVPESATP AALGYAQKCG LPYVEVLCKN 350
RYVGRTFIQP NMRLRQLGVA KKFGVLSDNF KGKRVVIIDD SIVRGNTISP 400
IIKLLRESGA KEVHIRVASP PIRFPCYMGI NIPTKEELIA NRPEFHDLAN 450
YIGADSVVYL SVEGLVSSVQ ESIKARKENE NSLKTQKSRV GKIGHCTACL 500
TGDYPVELEW 510
Length:510
Mass (Da):56,257
Last modified:December 1, 1992 - v1
Checksum:iF4371FE1FEC7C744
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M60069 mRNA. Translation: AAA62736.1.
L12533 Unassigned DNA. Translation: AAA17895.1.
PIRiA38337.
RefSeqiNP_001004401.1. NM_001004401.1.
UniGeneiGga.2870.

Genome annotation databases

GeneIDi422743.
KEGGigga:422743.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M60069 mRNA. Translation: AAA62736.1 .
L12533 Unassigned DNA. Translation: AAA17895.1 .
PIRi A38337.
RefSeqi NP_001004401.1. NM_001004401.1.
UniGenei Gga.2870.

3D structure databases

ProteinModelPortali P28173.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C44.001.

Proteomic databases

PaxDbi P28173.
PRIDEi P28173.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 422743.
KEGGi gga:422743.

Organism-specific databases

CTDi 5471.

Phylogenomic databases

eggNOGi COG0034.
HOGENOMi HOG000033688.
HOVERGENi HBG002589.
InParanoidi P28173.
KOi K00764.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00124 .
Reactomei REACT_116007. De novo synthesis of IMP.

Miscellaneous databases

NextBioi 20825333.
PROi P28173.

Family and domain databases

Gene3Di 3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
InterProi IPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view ]
Pfami PF00310. GATase_2. 2 hits.
PF00156. Pribosyltran. 1 hit.
[Graphical view ]
PIRSFi PIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMi SSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsi TIGR01134. purF. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and expression of avian glutamine phosphoribosylpyrophosphate amidotransferase. Conservation of a bacterial propeptide sequence supports a role for posttranslational processing."
    Zhou G., Dixon J.E., Zalkin H.
    J. Biol. Chem. 265:21152-21159(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Coexpression of two closely linked avian genes for purine nucleotide synthesis from a bidirectional promoter."
    Gavalas A., Dixon J.E., Brayton K.A., Zalkin H.
    Mol. Cell. Biol. 13:4784-4792(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 1-42.
  3. Erratum
    Gavalas A., Dixon J.E., Brayton K.A., Zalkin H.
    Mol. Cell. Biol. 13:7977-7977(1993)

Entry informationi

Entry nameiPUR1_CHICK
AccessioniPrimary (citable) accession number: P28173
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: September 3, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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