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P28173 (PUR1_CHICK) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Amidophosphoribosyltransferase

Short name=ATase
EC=2.4.2.14
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase
Short name=GPAT
Gene names
Name:PPAT
Synonyms:GPAT
OrganismGallus gallus (Chicken) [Reference proteome]
Taxonomic identifier9031 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus

Protein attributes

Sequence length510 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Binds 1 4Fe-4S cluster per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.

Subunit structure

Homotetramer.

Sequence similarities

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.

Contains 1 glutamine amidotransferase type-2 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Propeptide1 – 1111 Probable
PRO_0000029281
Chain12 – 510499Amidophosphoribosyltransferase
PRO_0000029282

Regions

Domain12 – 261250Glutamine amidotransferase type-2

Sites

Active site121For GATase activity By similarity
Metal binding2801Iron-sulfur (4Fe-4S) By similarity
Metal binding3271Magnesium By similarity
Metal binding3891Magnesium By similarity
Metal binding3901Magnesium By similarity
Metal binding4261Iron-sulfur (4Fe-4S) By similarity
Metal binding4961Iron-sulfur (4Fe-4S) By similarity
Metal binding4991Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
P28173 [UniParc].

Last modified December 1, 1992. Version 1.
Checksum: F4371FE1FEC7C744

FASTA51056,257
        10         20         30         40         50         60 
MELEELGIRE ECGVFGCIAA GVWPTELDVP HVITLGLVGL QHRGQESAGI VTSDGESSQA 

        70         80         90        100        110        120 
FKVHKGMGLI NHVFNADSLK KLYVSNLGIG HTRYSTSGIS ELQNCQPFVV ETLHGKIAVA 

       130        140        150        160        170        180 
HNGELTNAVR LRRKLMRHGV GLSTSSDSEL ITQLLAFTPP LENDDTADWV ARIKNLMNET 

       190        200        210        220        230        240 
PTSYSLLIMH KDIIYAVRDP YGNRPLCIGR LIPVGDINGK GKDNSETEGW VVSSESCSFL 

       250        260        270        280        290        300 
SIGAEYYREV LPGEIVKISR YDVQTLDVVP RPEGDPSAFC IFEYVYFARP DSIFEGQMVY 

       310        320        330        340        350        360 
SVRRRCGQQL AIEAPVEADL VSTVPESATP AALGYAQKCG LPYVEVLCKN RYVGRTFIQP 

       370        380        390        400        410        420 
NMRLRQLGVA KKFGVLSDNF KGKRVVIIDD SIVRGNTISP IIKLLRESGA KEVHIRVASP 

       430        440        450        460        470        480 
PIRFPCYMGI NIPTKEELIA NRPEFHDLAN YIGADSVVYL SVEGLVSSVQ ESIKARKENE 

       490        500        510 
NSLKTQKSRV GKIGHCTACL TGDYPVELEW 

« Hide

References

[1]"Cloning and expression of avian glutamine phosphoribosylpyrophosphate amidotransferase. Conservation of a bacterial propeptide sequence supports a role for posttranslational processing."
Zhou G., Dixon J.E., Zalkin H.
J. Biol. Chem. 265:21152-21159(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Coexpression of two closely linked avian genes for purine nucleotide synthesis from a bidirectional promoter."
Gavalas A., Dixon J.E., Brayton K.A., Zalkin H.
Mol. Cell. Biol. 13:4784-4792(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE OF 1-42.
[3]Erratum
Gavalas A., Dixon J.E., Brayton K.A., Zalkin H.
Mol. Cell. Biol. 13:7977-7977(1993)

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M60069 mRNA. Translation: AAA62736.1.
L12533 Unassigned DNA. Translation: AAA17895.1.
PIRA38337.
RefSeqNP_001004401.1. NM_001004401.1.
UniGeneGga.2870.

3D structure databases

ProteinModelPortalP28173.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSC44.001.

Proteomic databases

PaxDbP28173.
PRIDEP28173.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID422743.
KEGGgga:422743.

Organism-specific databases

CTD5471.

Phylogenomic databases

eggNOGCOG0034.
HOGENOMHOG000033688.
HOVERGENHBG002589.
InParanoidP28173.
KOK00764.

Enzyme and pathway databases

ReactomeREACT_115655. Metabolism.
UniPathwayUPA00074; UER00124.

Family and domain databases

InterProIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR000836. PRibTrfase_dom.
[Graphical view]
PfamPF00310. GATase_2. 2 hits.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFPIRSF000485. Amd_phspho_trans. 1 hit.
TIGRFAMsTIGR01134. purF. 1 hit.
PROSITEPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20825333.
PROP28173.

Entry information

Entry namePUR1_CHICK
AccessionPrimary (citable) accession number: P28173
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: April 16, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways