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Protein

Zinc finger protein 1

Gene

zfh1

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the development of the embryonic central nervous system, embryonic mesoderm and adult musculature.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri74 – 97C2H2-type 1PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri289 – 311C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri324 – 346C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri355 – 377C2H2-type 4PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri383 – 407C2H2-type 5PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri628 – 651C2H2-type 6PROSITE-ProRule annotationAdd BLAST24
DNA bindingi699 – 758HomeoboxPROSITE-ProRule annotationAdd BLAST60
Zinc fingeri967 – 989C2H2-type 7PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri995 – 1017C2H2-type 8PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri1023 – 1044C2H2-type 9PROSITE-ProRule annotationAdd BLAST22

GO - Molecular functioni

  • DNA binding Source: FlyBase
  • metal ion binding Source: UniProtKB-KW
  • RNA polymerase II regulatory region sequence-specific DNA binding Source: FlyBase
  • transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding Source: FlyBase

GO - Biological processi

  • antimicrobial humoral response Source: FlyBase
  • garland nephrocyte differentiation Source: FlyBase
  • germ cell migration Source: FlyBase
  • gonad development Source: FlyBase
  • heart development Source: FlyBase
  • hemocyte development Source: FlyBase
  • lymph gland development Source: FlyBase
  • mesoderm development Source: FlyBase
  • motor neuron axon guidance Source: FlyBase
  • negative regulation of transcription from RNA polymerase II promoter Source: FlyBase
  • nervous system development Source: FlyBase
  • pole cell migration Source: FlyBase
  • somatic stem cell division Source: FlyBase
Complete GO annotation...

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

SignaLinkiP28166.

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein 1
Alternative name(s):
Zinc finger homeodomain protein 1
Gene namesi
Name:zfh1
Synonyms:zfh-1
ORF Names:CG1322
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0004606. zfh1.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000472411 – 1054Zinc finger protein 1Add BLAST1054

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei582Phosphoserine1 Publication1
Modified residuei586Phosphoserine1 Publication1
Modified residuei934Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP28166.
PRIDEiP28166.

PTM databases

iPTMnetiP28166.

Expressioni

Tissue specificityi

Mesoderm and mesodermally-derived structures in the embryo including the dorsal vessel, support cells of the gonads, and segment-specific arrays of adult muscle precursor. Also identified in motor neurons of developing CNS.1 Publication

Gene expression databases

BgeeiFBgn0004606.
GenevisibleiP28166. DM.

Interactioni

Protein-protein interaction databases

BioGridi68501. 14 interactors.
IntActiP28166. 5 interactors.
MINTiMINT-94472.
STRINGi7227.FBpp0303607.

Structurei

3D structure databases

ProteinModelPortaliP28166.
SMRiP28166.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi222 – 246Gln-rich (OPA repeat)Add BLAST25

Sequence similaritiesi

Contains 9 C2H2-type zinc fingers.PROSITE-ProRule annotation
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri74 – 97C2H2-type 1PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri289 – 311C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri324 – 346C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri355 – 377C2H2-type 4PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri383 – 407C2H2-type 5PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri628 – 651C2H2-type 6PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri967 – 989C2H2-type 7PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri995 – 1017C2H2-type 8PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri1023 – 1044C2H2-type 9PROSITE-ProRule annotationAdd BLAST22

Keywords - Domaini

Homeobox, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG3623. Eukaryota.
ENOG410ZFMZ. LUCA.
GeneTreeiENSGT00630000089829.
InParanoidiP28166.
KOiK09299.
OrthoDBiEOG091G0L5U.
PhylomeDBiP28166.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.30.160.60. 5 hits.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF13912. zf-C2H2_6. 2 hits.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
SM00355. ZnF_C2H2. 9 hits.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 6 hits.
PS50157. ZINC_FINGER_C2H2_2. 9 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform B (identifier: P28166-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLSCLAPSSS RFGQEDTIIQ QSMPSTSPFA MQFPSLASTL LHHNQSPKHS
60 70 80 90 100
NPGSSGIQDA HPNQPGAAAD AFLVKCTQCH KRFPEYQSLS EHIASEHPHD
110 120 130 140 150
KLNCGAAQPE SDAEDEQSNM SGSSRRYAKS PLASNNNSST ANANNNSTSS
160 170 180 190 200
QSMNNNSELA KNHNSANKMS PMCSPGSLTP GDLFAQLQHP PPQLPPHLHA
210 220 230 240 250
QFMAAAASLA MQSARTASSP SQQQQQQLQQ QQQLQQQQQH QMAMQQLLPP
260 270 280 290 300
QLPGSNSSVG SNSAYDLDLS APRSTSSPGS TTGDLSGAYP CMQCTASFAS
310 320 330 340 350
REQLEQHEQL HSPCGPAAVS NVSQTCRICH KAFANVYRLQ RHMISHDESA
360 370 380 390 400
LLRKFKCKEC DKAFKFKHHL KEHVRIHSGE KPFGCDNCGK RFSHSGSFSS
410 420 430 440 450
HMTSKKCISM GLKLNNNRAL LKRLEKSPGS ASSASRRSPS DHGKGKLPEQ
460 470 480 490 500
PSLPGLPHPM SYFASDAQVQ GGSAAPAPFP PFHPNYMNAA LLAFPHNFMA
510 520 530 540 550
AAAGLDPRVH PYSIQRLLQL SAAGQQQREE EREEQQKQQQ HDEEETPDEP
560 570 580 590 600
KLVMDIEEPE TKEMAPTPEA TEAATPIKRE ESREASPDPE SYRSSSQAIK
610 620 630 640 650
QEQEPLNVAE ERQTPVEEHA PVEHAADLRC SRCSKQFNHP TELVQHEKVL
660 670 680 690 700
CGLIKEELEQ HFQQQQATSF ALASASEEDE EDEEMDVEEE PRQESGERKV
710 720 730 740 750
RVRTAINEEQ QQQLKQHYSL NARPSRDEFR MIAARLQLDP RVVQVWFQNN
760 770 780 790 800
RSRERKMQSF QNNQAAGAAP PMPIDSQASL TREDQPLDLS VKRDPLTPKS
810 820 830 840 850
ESSPPYIAPP SGEALNPEAI NLSRKFSTSA SMSPASISPS SAAALYFGAA
860 870 880 890 900
PPPSPPNSQL DSTPRSGQAF PGLPPYMLPM SLPMEALFKM RPGGDFASNH
910 920 930 940 950
ALMNSIKLPD YRGTSLSPGG SEKRSWRDDD SRISHEDEFG AGVLMPPKPR
960 970 980 990 1000
RGKAETHGHA GDPDLPYVCD QCDKAFAKQS SLARHKYEHS GQRPYQCIEC
1010 1020 1030 1040 1050
PKAFKHKHHL TEHKRLHSGE KPFQCSKCLK RFSHSGSYSQ HMNHRYSYCK

PYRE
Length:1,054
Mass (Da):116,598
Last modified:March 15, 2004 - v2
Checksum:i5189AB2214AB5B8B
GO
Isoform A (identifier: P28166-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-307: Missing.
     308-324: EQLHSPCGPAAVSNVSQ → MSAAACLLSSSTSSFEK

Note: No experimental confirmation available.
Show »
Length:747
Mass (Da):83,777
Checksum:i2F34280BBA626A89
GO

Sequence cautioni

The sequence AAM50023 differs from that shown. Contaminating sequence.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti78Q → K in AAR82746 (Ref. 4) Curated1
Sequence conflicti147S → T in AAR82746 (Ref. 4) Curated1
Sequence conflicti239Q → QMQQQQQ in AAA29050 (PubMed:1680376).Curated1
Sequence conflicti625A → S in AAA29050 (PubMed:1680376).Curated1
Sequence conflicti625A → S in AAR82746 (Ref. 4) Curated1
Sequence conflicti625A → S in AAM50023 (PubMed:12537569).Curated1
Sequence conflicti954A → V in AAA29050 (PubMed:1680376).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0096701 – 307Missing in isoform A. 1 PublicationAdd BLAST307
Alternative sequenceiVSP_009671308 – 324EQLHS…SNVSQ → MSAAACLLSSSTSSFEK in isoform A. 1 PublicationAdd BLAST17

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63449 Genomic DNA. Translation: AAA29050.1.
AE014297 Genomic DNA. Translation: AAF57083.1.
AE014297 Genomic DNA. Translation: AAF57084.1.
BT003277 mRNA. Translation: AAO25034.1.
BT011080 mRNA. Translation: AAR82746.1.
AY118654 mRNA. Translation: AAM50023.1. Sequence problems.
PIRiS33641.
RefSeqiNP_476850.1. NM_057502.5. [P28166-1]
NP_733402.1. NM_170523.3. [P28166-2]
UniGeneiDm.4708.

Genome annotation databases

EnsemblMetazoaiFBtr0085701; FBpp0085063; FBgn0004606. [P28166-1]
GeneIDi43650.
KEGGidme:Dmel_CG1322.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63449 Genomic DNA. Translation: AAA29050.1.
AE014297 Genomic DNA. Translation: AAF57083.1.
AE014297 Genomic DNA. Translation: AAF57084.1.
BT003277 mRNA. Translation: AAO25034.1.
BT011080 mRNA. Translation: AAR82746.1.
AY118654 mRNA. Translation: AAM50023.1. Sequence problems.
PIRiS33641.
RefSeqiNP_476850.1. NM_057502.5. [P28166-1]
NP_733402.1. NM_170523.3. [P28166-2]
UniGeneiDm.4708.

3D structure databases

ProteinModelPortaliP28166.
SMRiP28166.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi68501. 14 interactors.
IntActiP28166. 5 interactors.
MINTiMINT-94472.
STRINGi7227.FBpp0303607.

PTM databases

iPTMnetiP28166.

Proteomic databases

PaxDbiP28166.
PRIDEiP28166.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0085701; FBpp0085063; FBgn0004606. [P28166-1]
GeneIDi43650.
KEGGidme:Dmel_CG1322.

Organism-specific databases

CTDi43650.
FlyBaseiFBgn0004606. zfh1.

Phylogenomic databases

eggNOGiKOG3623. Eukaryota.
ENOG410ZFMZ. LUCA.
GeneTreeiENSGT00630000089829.
InParanoidiP28166.
KOiK09299.
OrthoDBiEOG091G0L5U.
PhylomeDBiP28166.

Enzyme and pathway databases

SignaLinkiP28166.

Miscellaneous databases

GenomeRNAii43650.
PROiP28166.

Gene expression databases

BgeeiFBgn0004606.
GenevisibleiP28166. DM.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.30.160.60. 5 hits.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF13912. zf-C2H2_6. 2 hits.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
SM00355. ZnF_C2H2. 9 hits.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 6 hits.
PS50157. ZINC_FINGER_C2H2_2. 9 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiZFH1_DROME
AccessioniPrimary (citable) accession number: P28166
Secondary accession number(s): Q59DT3
, Q6NP51, Q8MSQ8, Q9VA39, Q9VA40
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: March 15, 2004
Last modified: November 2, 2016
This is version 157 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.