Reviewed, UniProtKB/Swiss-Prot P28166 (ZFH1_DROME)

Names and origin

Protein names

Recommended name:
    Zinc finger protein 1
Alternative name(s):
    Zinc finger homeodomain protein 1

Gene names

Name:	zfh1
Synonyms:	zfh-1
ORF Names:	CG1322

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	1054 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the development of the embryonic central nervous system, embryonic mesoderm and adult musculature. Ref.1
Subcellular location	Nucleus Probable.
Tissue specificity	Mesoderm and mesodermally-derived structures in the embryo including the dorsal vessel, support cells of the gonads, and segment-specific arrays of adult muscle precursor. Also identified in motor neurons of developing CNS. Ref.1
Sequence similarities	Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family. Contains 9 C2H2-type zinc fingers. Contains 1 homeobox DNA-binding domain.
Sequence caution	The sequence AAM50023.1 differs from that shown. Reason: Frameshift at position 539.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Homeobox Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	antimicrobial humoral response Inferred from mutant phenotype. Source: FlyBase garland cell differentiation Inferred from mutant phenotype. Source: FlyBase gonad development Traceable author statement. Source: FlyBase heart development Non-traceable author statement. Source: FlyBase lymph gland development Inferred from mutant phenotype. Source: FlyBase mesoderm development Inferred from expression pattern. Source: FlyBase motor axon guidance Inferred from mutant phenotype. Source: FlyBase pole cell migration Traceable author statement. Source: FlyBase regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro transcription factor activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q8IPY9	1	EBI-142903,EBI-83191
Bj1	P25171	1	EBI-142903,EBI-181948

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P28166-1)

Also known as: B;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P28166-2)

Also known as: A;

The sequence of this isoform differs from the canonical sequence as follows:
     1-307: Missing.
     308-324: EQLHSPCGPAAVSNVSQ → MSAAACLLSSSTSSFEK

Note: No experimental confirmation available.

Isoform 3 (identifier: P28166-3)

Also known as: C;

The sequence of this isoform differs from the canonical sequence as follows:
     344-538: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1054	1054	Zinc finger protein 1		PRO_0000047241
Regions
	Zinc finger	74 – 97	24	C2H2-type 1
	Zinc finger	289 – 311	23	C2H2-type 2
	Zinc finger	324 – 346	23	C2H2-type 3
	Zinc finger	355 – 377	23	C2H2-type 4
	Zinc finger	383 – 407	25	C2H2-type 5
	Zinc finger	628 – 651	24	C2H2-type 6
	DNA binding	699 – 758	60	Homeobox
	Zinc finger	967 – 989	23	C2H2-type 7
	Zinc finger	995 – 1017	23	C2H2-type 8
	Zinc finger	1023 – 1044	22	C2H2-type 9
	Compositional bias	222 – 246	25	Gln-rich (OPA repeat)
Amino acid modifications
	Modified residue	582	1	Phosphoserine Ref.6
	Modified residue	586	1	Phosphoserine Ref.6
	Modified residue	934	1	Phosphoserine Ref.6
Natural variations
	Alternative sequence	1 – 307	307	Missing in isoform 2.		VSP_009670
	Alternative sequence	308 – 324	17	EQLHS…SNVSQ → MSAAACLLSSSTSSFEK in isoform 2.		VSP_009671
	Alternative sequence	344 – 538	195	Missing in isoform 3.		VSP_009672
Experimental info
	Sequence conflict	78	1	Q → K in AAR82746. Ref.5
	Sequence conflict	147	1	S → T in AAR82746. Ref.5
	Sequence conflict	239	1	Q → QMQQQQQ Ref.1
	Sequence conflict	625	1	A → S Ref.1
	Sequence conflict	625	1	A → S Ref.4
	Sequence conflict	625	1	A → S in AAR82746. Ref.5
	Sequence conflict	954	1	A → V in AAA29050. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (B) [UniParc]. Last modified March 15, 2004. Version 2. Checksum: 5189AB2214AB5B8B	FASTA	1,054	116,598
	10 20 30 40 50 60 MLSCLAPSSS RFGQEDTIIQ QSMPSTSPFA MQFPSLASTL LHHNQSPKHS NPGSSGIQDA 70 80 90 100 110 120 HPNQPGAAAD AFLVKCTQCH KRFPEYQSLS EHIASEHPHD KLNCGAAQPE SDAEDEQSNM 130 140 150 160 170 180 SGSSRRYAKS PLASNNNSST ANANNNSTSS QSMNNNSELA KNHNSANKMS PMCSPGSLTP 190 200 210 220 230 240 GDLFAQLQHP PPQLPPHLHA QFMAAAASLA MQSARTASSP SQQQQQQLQQ QQQLQQQQQH 250 260 270 280 290 300 QMAMQQLLPP QLPGSNSSVG SNSAYDLDLS APRSTSSPGS TTGDLSGAYP CMQCTASFAS 310 320 330 340 350 360 REQLEQHEQL HSPCGPAAVS NVSQTCRICH KAFANVYRLQ RHMISHDESA LLRKFKCKEC 370 380 390 400 410 420 DKAFKFKHHL KEHVRIHSGE KPFGCDNCGK RFSHSGSFSS HMTSKKCISM GLKLNNNRAL 430 440 450 460 470 480 LKRLEKSPGS ASSASRRSPS DHGKGKLPEQ PSLPGLPHPM SYFASDAQVQ GGSAAPAPFP 490 500 510 520 530 540 PFHPNYMNAA LLAFPHNFMA AAAGLDPRVH PYSIQRLLQL SAAGQQQREE EREEQQKQQQ 550 560 570 580 590 600 HDEEETPDEP KLVMDIEEPE TKEMAPTPEA TEAATPIKRE ESREASPDPE SYRSSSQAIK 610 620 630 640 650 660 QEQEPLNVAE ERQTPVEEHA PVEHAADLRC SRCSKQFNHP TELVQHEKVL CGLIKEELEQ 670 680 690 700 710 720 HFQQQQATSF ALASASEEDE EDEEMDVEEE PRQESGERKV RVRTAINEEQ QQQLKQHYSL 730 740 750 760 770 780 NARPSRDEFR MIAARLQLDP RVVQVWFQNN RSRERKMQSF QNNQAAGAAP PMPIDSQASL 790 800 810 820 830 840 TREDQPLDLS VKRDPLTPKS ESSPPYIAPP SGEALNPEAI NLSRKFSTSA SMSPASISPS 850 860 870 880 890 900 SAAALYFGAA PPPSPPNSQL DSTPRSGQAF PGLPPYMLPM SLPMEALFKM RPGGDFASNH 910 920 930 940 950 960 ALMNSIKLPD YRGTSLSPGG SEKRSWRDDD SRISHEDEFG AGVLMPPKPR RGKAETHGHA 970 980 990 1000 1010 1020 GDPDLPYVCD QCDKAFAKQS SLARHKYEHS GQRPYQCIEC PKAFKHKHHL TEHKRLHSGE 1030 1040 1050 KPFQCSKCLK RFSHSGSYSQ HMNHRYSYCK PYRE
	Isoform 2 (A). Checksum: 2F34280BBA626A89	FASTA	747	83,777
	10 20 30 40 50 60 MSAAACLLSS STSSFEKTCR ICHKAFANVY RLQRHMISHD ESALLRKFKC KECDKAFKFK 70 80 90 100 110 120 HHLKEHVRIH SGEKPFGCDN CGKRFSHSGS FSSHMTSKKC ISMGLKLNNN RALLKRLEKS 130 140 150 160 170 180 PGSASSASRR SPSDHGKGKL PEQPSLPGLP HPMSYFASDA QVQGGSAAPA PFPPFHPNYM 190 200 210 220 230 240 NAALLAFPHN FMAAAAGLDP RVHPYSIQRL LQLSAAGQQQ REEEREEQQK QQQHDEEETP 250 260 270 280 290 300 DEPKLVMDIE EPETKEMAPT PEATEAATPI KREESREASP DPESYRSSSQ AIKQEQEPLN 310 320 330 340 350 360 VAEERQTPVE EHAPVEHAAD LRCSRCSKQF NHPTELVQHE KVLCGLIKEE LEQHFQQQQA 370 380 390 400 410 420 TSFALASASE EDEEDEEMDV EEEPRQESGE RKVRVRTAIN EEQQQQLKQH YSLNARPSRD 430 440 450 460 470 480 EFRMIAARLQ LDPRVVQVWF QNNRSRERKM QSFQNNQAAG AAPPMPIDSQ ASLTREDQPL 490 500 510 520 530 540 DLSVKRDPLT PKSESSPPYI APPSGEALNP EAINLSRKFS TSASMSPASI SPSSAAALYF 550 560 570 580 590 600 GAAPPPSPPN SQLDSTPRSG QAFPGLPPYM LPMSLPMEAL FKMRPGGDFA SNHALMNSIK 610 620 630 640 650 660 LPDYRGTSLS PGGSEKRSWR DDDSRISHED EFGAGVLMPP KPRRGKAETH GHAGDPDLPY 670 680 690 700 710 720 VCDQCDKAFA KQSSLARHKY EHSGQRPYQC IECPKAFKHK HHLTEHKRLH SGEKPFQCSK 730 740 CLKRFSHSGS YSQHMNHRYS YCKPYRE
	Isoform 3 (C). Checksum: 9A9ADF04C8886BF3	FASTA	859	95,032
	10 20 30 40 50 60 MLSCLAPSSS RFGQEDTIIQ QSMPSTSPFA MQFPSLASTL LHHNQSPKHS NPGSSGIQDA 70 80 90 100 110 120 HPNQPGAAAD AFLVKCTQCH KRFPEYQSLS EHIASEHPHD KLNCGAAQPE SDAEDEQSNM 130 140 150 160 170 180 SGSSRRYAKS PLASNNNSST ANANNNSTSS QSMNNNSELA KNHNSANKMS PMCSPGSLTP 190 200 210 220 230 240 GDLFAQLQHP PPQLPPHLHA QFMAAAASLA MQSARTASSP SQQQQQQLQQ QQQLQQQQQH 250 260 270 280 290 300 QMAMQQLLPP QLPGSNSSVG SNSAYDLDLS APRSTSSPGS TTGDLSGAYP CMQCTASFAS 310 320 330 340 350 360 REQLEQHEQL HSPCGPAAVS NVSQTCRICH KAFANVYRLQ RHMQQHDEEE TPDEPKLVMD 370 380 390 400 410 420 IEEPETKEMA PTPEATEAAT PIKREESREA SPDPESYRSS SQAIKQEQEP LNVAEERQTP 430 440 450 460 470 480 VEEHAPVEHA ADLRCSRCSK QFNHPTELVQ HEKVLCGLIK EELEQHFQQQ QATSFALASA 490 500 510 520 530 540 SEEDEEDEEM DVEEEPRQES GERKVRVRTA INEEQQQQLK QHYSLNARPS RDEFRMIAAR 550 560 570 580 590 600 LQLDPRVVQV WFQNNRSRER KMQSFQNNQA AGAAPPMPID SQASLTREDQ PLDLSVKRDP 610 620 630 640 650 660 LTPKSESSPP YIAPPSGEAL NPEAINLSRK FSTSASMSPA SISPSSAAAL YFGAAPPPSP 670 680 690 700 710 720 PNSQLDSTPR SGQAFPGLPP YMLPMSLPME ALFKMRPGGD FASNHALMNS IKLPDYRGTS 730 740 750 760 770 780 LSPGGSEKRS WRDDDSRISH EDEFGAGVLM PPKPRRGKAE THGHAGDPDL PYVCDQCDKA 790 800 810 820 830 840 FAKQSSLARH KYEHSGQRPY QCIECPKAFK HKHHLTEHKR LHSGEKPFQC SKCLKRFSHS 850 GSYSQHMNHR YSYCKPYRE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Drosophila zfh-1 and zfh-2 genes encode novel proteins containing both zinc-finger and homeodomain motifs." Fortini M.E., Lai Z., Rubin G.M. Mech. Dev. 34:113-122(1991) [PubMed: 1680376] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Strain: Berkeley. Tissue: Embryo.
[5]	Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A. , Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E. Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 30-1054 (ISOFORM 1). Strain: Berkeley. Tissue: Embryo.
[6]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed: 18327897] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-582; SER-586 AND SER-934, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M63449 Genomic DNA. Translation: AAA29050.1. AE014297 Genomic DNA. Translation: AAF57083.1. AE014297 Genomic DNA. Translation: AAF57084.1. AE014297 Genomic DNA. Translation: AAN14258.2. AY118654 mRNA. Translation: AAM50023.1. Frameshift. BT003277 mRNA. Translation: AAO25034.1. BT011080 mRNA. Translation: AAR82746.1.
PIR	S33641.
RefSeq	NP_476850.1. NP_733401.2. NP_733402.1.
UniGene	Dm.4708
3D structure databases
HSSP	HSSP built from PDB template 1ZFD based on UniProtKB P08153.
ModBase	Search...
Protein-protein interaction databases
IntAct	P28166. 12 interactions.
Genome annotation databases
Ensembl	FBgn0004606. Drosophila melanogaster. [Contig view]
GeneID	43650.
KEGG	dme:Dmel_CG1322.
NMPDR	fig|7227.3.peg.15505.
Organism-specific databases
FlyBase	FBgn0004606. zfh1.
Phylogenomic databases
HOGENOM	P28166.
OMA	P28166. SERSICL.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-014232-MON.
Gene expression databases
ArrayExpress	P28166.
GermOnline	CG1322. Drosophila melanogaster.
Family and domain databases
InterPro	IPR001356. Homeobox. IPR017970. Homeobox_CS. IPR012287. Homeodomain-rel. IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
Gene3D	G3DSA:1.10.10.60. Homeodomain-rel. 1 hit. G3DSA:3.30.160.60. Znf_C2H2/integrase_DNA-bd. 3 hits.
Pfam	PF00046. Homeobox. 1 hit. PF00096. zf-C2H2. 8 hits. [Graphical view]
ProDom	PD000010. Homeobox. 1 hit. PD000003. Znf_C2H2. 2 hits. [Graphical view] [Entries sharing at least one domain]
SMART	SM00389. HOX. 1 hit. SM00355. ZnF_C2H2. 9 hits. [Graphical view]
PROSITE	PS00027. HOMEOBOX_1. 1 hit. PS50071. HOMEOBOX_2. 1 hit. PS00028. ZINC_FINGER_C2H2_1. 6 hits. PS50157. ZINC_FINGER_C2H2_2. 9 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	835065.

Entry information

Entry name

ZFH1_DROME

Accession

Primary (citable) accession number: P28166
Secondary accession number(s): Q59DT3 , Q6NP51, Q8MSQ8, Q9VA39, Q9VA40

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	March 15, 2004
Last modified:	June 16, 2009
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project