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Protein

Glutathione S-transferase Mu 2

Gene

GSTM2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.1 Publication

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei50GlutathioneBy similarity1
Binding sitei116SubstrateBy similarity1
Sitei210Important for substrate specificity1

GO - Molecular functioni

  • enzyme binding Source: BHF-UCL
  • glutathione binding Source: BHF-UCL
  • glutathione peroxidase activity Source: BHF-UCL
  • glutathione transferase activity Source: UniProtKB
  • protein homodimerization activity Source: BHF-UCL
  • signaling receptor binding Source: BHF-UCL

GO - Biological processi

  • cellular detoxification of nitrogen compound Source: BHF-UCL
  • cellular response to caffeine Source: BHF-UCL
  • glutathione derivative biosynthetic process Source: Reactome
  • glutathione metabolic process Source: UniProtKB
  • linoleic acid metabolic process Source: BHF-UCL
  • negative regulation of ryanodine-sensitive calcium-release channel activity Source: BHF-UCL
  • nitrobenzene metabolic process Source: BHF-UCL
  • positive regulation of ryanodine-sensitive calcium-release channel activity Source: BHF-UCL
  • regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion Source: BHF-UCL
  • regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum Source: BHF-UCL
  • regulation of skeletal muscle contraction by regulation of release of sequestered calcium ion Source: BHF-UCL
  • relaxation of cardiac muscle Source: BHF-UCL
  • xenobiotic catabolic process Source: UniProtKB

Keywordsi

Molecular functionTransferase

Enzyme and pathway databases

BRENDAi2.5.1.18 2681
ReactomeiR-HSA-156590 Glutathione conjugation
SABIO-RKP28161

Chemistry databases

SwissLipidsiSLP:000001614

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase Mu 2 (EC:2.5.1.18)
Alternative name(s):
GST class-mu 2
GSTM2-2
Gene namesi
Name:GSTM2
Synonyms:GST4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000213366.12
HGNCiHGNC:4634 GSTM2
MIMi138380 gene
neXtProtiNX_P28161

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi210T → C, F, H, I, K, L, R, Y or W: Reduced enzyme activity. 1 Publication1

Organism-specific databases

DisGeNETi2946
OpenTargetsiENSG00000213366
PharmGKBiPA29023

Chemistry databases

ChEMBLiCHEMBL4589
DrugBankiDB00143 Glutathione
DB03310 Oxidized Glutathione Disulfide
DB04701 S-METHYL-GLUTATHIONE

Polymorphism and mutation databases

BioMutaiGSTM2
DMDMi232204

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001858181 – 218Glutathione S-transferase Mu 2Add BLAST218

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei27PhosphoserineBy similarity1
Modified residuei44PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP28161
MaxQBiP28161
PaxDbiP28161
PeptideAtlasiP28161
PRIDEiP28161

2D gel databases

OGPiP28161
REPRODUCTION-2DPAGEP28161

PTM databases

iPTMnetiP28161
PhosphoSitePlusiP28161

Expressioni

Tissue specificityi

Muscle.

Gene expression databases

BgeeiENSG00000213366
CleanExiHS_GSTM2
ExpressionAtlasiP28161 baseline and differential
GenevisibleiP28161 HS

Organism-specific databases

HPAiCAB040547
HPA055972
HPA055973

Interactioni

Subunit structurei

Homodimer.3 Publications

GO - Molecular functioni

  • enzyme binding Source: BHF-UCL
  • protein homodimerization activity Source: BHF-UCL
  • signaling receptor binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi109201, 6 interactors
IntActiP28161, 2 interactors
STRINGi9606.ENSP00000241337

Chemistry databases

BindingDBiP28161

Structurei

Secondary structure

1218
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 11Combined sources9
Helixi12 – 14Combined sources3
Helixi15 – 23Combined sources9
Beta strandi28 – 33Combined sources6
Turni38 – 41Combined sources4
Helixi44 – 47Combined sources4
Turni48 – 51Combined sources4
Beta strandi60 – 65Combined sources6
Beta strandi68 – 72Combined sources5
Helixi73 – 83Combined sources11
Helixi91 – 116Combined sources26
Helixi120 – 142Combined sources23
Beta strandi150 – 152Combined sources3
Helixi155 – 170Combined sources16
Turni172 – 177Combined sources6
Helixi179 – 190Combined sources12
Helixi192 – 199Combined sources8
Beta strandi200 – 202Combined sources3
Beta strandi205 – 207Combined sources3
Turni208 – 212Combined sources5
Beta strandi214 – 216Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HNAX-ray1.85A2-218[»]
1HNBX-ray3.50A/B2-218[»]
1HNCX-ray3.00A/B/C/D2-218[»]
1XW5X-ray1.80A/B2-218[»]
1YKCX-ray2.10A/B2-218[»]
2AB6X-ray2.50A/B/C/D2-218[»]
2C4JX-ray1.35A/B/C/D2-218[»]
2GTUX-ray2.55A/B2-218[»]
3GTUX-ray2.80A/C2-218[»]
3GURX-ray2.50A/B/C/D2-218[»]
5HWLX-ray1.60A/B2-218[»]
ProteinModelPortaliP28161
SMRiP28161
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP28161

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 88GST N-terminalAdd BLAST87
Domaini90 – 208GST C-terminalAdd BLAST119

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni7 – 8Glutathione binding2 Publications2
Regioni43 – 46Glutathione binding2 Publications4
Regioni59 – 60Glutathione binding2 Publications2
Regioni72 – 73Glutathione binding2 Publications2

Sequence similaritiesi

Belongs to the GST superfamily. Mu family.Curated

Phylogenomic databases

eggNOGiENOG410IN5J Eukaryota
ENOG4110YU0 LUCA
GeneTreeiENSGT00550000074559
HOGENOMiHOG000115735
HOVERGENiHBG106842
InParanoidiP28161
KOiK00799
OMAiEFHARIG
PhylomeDBiP28161
TreeFamiTF353040

Family and domain databases

InterProiView protein in InterPro
IPR010987 Glutathione-S-Trfase_C-like
IPR036282 Glutathione-S-Trfase_C_sf
IPR004045 Glutathione_S-Trfase_N
IPR004046 GST_C
IPR003081 GST_mu
IPR036249 Thioredoxin-like_sf
PfamiView protein in Pfam
PF00043 GST_C, 1 hit
PF02798 GST_N, 1 hit
PRINTSiPR01267 GSTRNSFRASEM
SUPFAMiSSF47616 SSF47616, 1 hit
SSF52833 SSF52833, 1 hit
PROSITEiView protein in PROSITE
PS50405 GST_CTER, 1 hit
PS50404 GST_NTER, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P28161-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPMTLGYWNI RGLAHSIRLL LEYTDSSYEE KKYTMGDAPD YDRSQWLNEK
60 70 80 90 100
FKLGLDFPNL PYLIDGTHKI TQSNAILRYI ARKHNLCGES EKEQIREDIL
110 120 130 140 150
ENQFMDSRMQ LAKLCYDPDF EKLKPEYLQA LPEMLKLYSQ FLGKQPWFLG
160 170 180 190 200
DKITFVDFIA YDVLERNQVF EPSCLDAFPN LKDFISRFEG LEKISAYMKS
210
SRFLPRPVFT KMAVWGNK
Length:218
Mass (Da):25,745
Last modified:January 23, 2007 - v2
Checksum:i15203709D4A63789
GO
Isoform 2 (identifier: P28161-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     190-218: GLEKISAYMKSSRFLPRPVFTKMAVWGNK → HS

Note: No experimental confirmation available.
Show »
Length:191
Mass (Da):22,644
Checksum:i9267243C014F878F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2P → S in AAV38750 (Ref. 3) Curated1
Sequence conflicti9N → D in AAV38750 (Ref. 3) Curated1
Sequence conflicti51F → L in BAG61446 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_049486173S → N. Corresponds to variant dbSNP:rs2229050Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_045614190 – 218GLEKI…VWGNK → HS in isoform 2. CuratedAdd BLAST29

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63509 mRNA Translation: AAA60963.1
AK299482 mRNA Translation: BAG61446.1
BT019947 mRNA Translation: AAV38750.1
AC000031 Genomic DNA No translation available.
AC000032 Genomic DNA No translation available.
BC105038 mRNA Translation: AAI05039.1
BC105066 mRNA Translation: AAI05067.1
CCDSiCCDS44192.1 [P28161-2]
CCDS808.1 [P28161-1]
PIRiA39375
RefSeqiNP_000839.1, NM_000848.3 [P28161-1]
NP_001135840.1, NM_001142368.1 [P28161-2]
UniGeneiHs.279837

Genome annotation databases

EnsembliENST00000241337; ENSP00000241337; ENSG00000213366 [P28161-1]
ENST00000442650; ENSP00000416883; ENSG00000213366 [P28161-2]
ENST00000460717; ENSP00000435910; ENSG00000213366 [P28161-2]
GeneIDi2946
KEGGihsa:2946
UCSCiuc001dyj.4 human [P28161-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiGSTM2_HUMAN
AccessioniPrimary (citable) accession number: P28161
Secondary accession number(s): B4DRY4
, E9PEM9, Q2M318, Q5TZY5, Q8WWE1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 185 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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