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Protein

Glutathione S-transferase Mu 2

Gene

GSTM2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.1 Publication

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei50GlutathioneBy similarity1
Binding sitei116SubstrateBy similarity1
Sitei210Important for substrate specificity1

GO - Molecular functioni

  • enzyme binding Source: BHF-UCL
  • glutathione binding Source: BHF-UCL
  • glutathione peroxidase activity Source: BHF-UCL
  • glutathione transferase activity Source: UniProtKB
  • protein homodimerization activity Source: BHF-UCL
  • receptor binding Source: BHF-UCL

GO - Biological processi

  • cellular detoxification of nitrogen compound Source: BHF-UCL
  • cellular response to caffeine Source: BHF-UCL
  • glutathione derivative biosynthetic process Source: Reactome
  • glutathione metabolic process Source: UniProtKB
  • linoleic acid metabolic process Source: BHF-UCL
  • negative regulation of ryanodine-sensitive calcium-release channel activity Source: BHF-UCL
  • nitrobenzene metabolic process Source: BHF-UCL
  • positive regulation of ryanodine-sensitive calcium-release channel activity Source: BHF-UCL
  • regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion Source: BHF-UCL
  • regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum Source: BHF-UCL
  • regulation of skeletal muscle contraction by regulation of release of sequestered calcium ion Source: BHF-UCL
  • relaxation of cardiac muscle Source: BHF-UCL
  • xenobiotic catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

BioCyciZFISH:HS09818-MONOMER.
BRENDAi2.5.1.18. 2681.
ReactomeiR-HSA-156590. Glutathione conjugation.
SABIO-RKP28161.

Chemistry databases

SwissLipidsiSLP:000001614.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase Mu 2 (EC:2.5.1.18)
Alternative name(s):
GST class-mu 2
GSTM2-2
Gene namesi
Name:GSTM2
Synonyms:GST4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:4634. GSTM2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: BHF-UCL
  • cytosol Source: Reactome
  • extracellular exosome Source: UniProtKB
  • sarcoplasmic reticulum Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi210T → C, F, H, I, K, L, R, Y or W: Reduced enzyme activity. 1 Publication1

Organism-specific databases

DisGeNETi2946.
OpenTargetsiENSG00000213366.
PharmGKBiPA29023.

Chemistry databases

ChEMBLiCHEMBL4589.
DrugBankiDB00143. Glutathione.

Polymorphism and mutation databases

BioMutaiGSTM2.
DMDMi232204.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001858182 – 218Glutathione S-transferase Mu 2Add BLAST217

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei27PhosphoserineBy similarity1
Modified residuei44PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP28161.
MaxQBiP28161.
PaxDbiP28161.
PeptideAtlasiP28161.
PRIDEiP28161.

2D gel databases

OGPiP28161.
REPRODUCTION-2DPAGEP28161.

PTM databases

iPTMnetiP28161.
PhosphoSitePlusiP28161.

Expressioni

Tissue specificityi

Muscle.

Gene expression databases

BgeeiENSG00000213366.
CleanExiHS_GSTM2.
ExpressionAtlasiP28161. baseline and differential.
GenevisibleiP28161. HS.

Organism-specific databases

HPAiCAB040547.
HPA055972.
HPA055973.

Interactioni

Subunit structurei

Homodimer.3 Publications

GO - Molecular functioni

  • enzyme binding Source: BHF-UCL
  • protein homodimerization activity Source: BHF-UCL
  • receptor binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi109201. 6 interactors.
IntActiP28161. 1 interactor.
STRINGi9606.ENSP00000241337.

Chemistry databases

BindingDBiP28161.

Structurei

Secondary structure

1218
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 11Combined sources9
Helixi12 – 14Combined sources3
Helixi15 – 23Combined sources9
Beta strandi28 – 33Combined sources6
Turni38 – 41Combined sources4
Helixi44 – 47Combined sources4
Turni48 – 51Combined sources4
Beta strandi60 – 65Combined sources6
Beta strandi68 – 72Combined sources5
Helixi73 – 83Combined sources11
Helixi91 – 116Combined sources26
Helixi120 – 142Combined sources23
Beta strandi150 – 152Combined sources3
Helixi155 – 170Combined sources16
Turni172 – 177Combined sources6
Helixi179 – 190Combined sources12
Helixi192 – 199Combined sources8
Beta strandi200 – 202Combined sources3
Beta strandi205 – 207Combined sources3
Turni208 – 212Combined sources5
Beta strandi214 – 216Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HNAX-ray1.85A2-218[»]
1HNBX-ray3.50A/B2-218[»]
1HNCX-ray3.00A/B/C/D2-218[»]
1XW5X-ray1.80A/B2-218[»]
1YKCX-ray2.10A/B2-218[»]
2AB6X-ray2.50A/B/C/D2-218[»]
2C4JX-ray1.35A/B/C/D2-218[»]
2GTUX-ray2.55A/B2-218[»]
3GTUX-ray2.80A/C2-218[»]
3GURX-ray2.50A/B/C/D2-218[»]
ProteinModelPortaliP28161.
SMRiP28161.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP28161.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 88GST N-terminalAdd BLAST87
Domaini90 – 208GST C-terminalAdd BLAST119

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni7 – 8Glutathione binding2 Publications2
Regioni43 – 46Glutathione binding2 Publications4
Regioni59 – 60Glutathione binding2 Publications2
Regioni72 – 73Glutathione binding2 Publications2

Sequence similaritiesi

Belongs to the GST superfamily. Mu family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

eggNOGiENOG410IN5J. Eukaryota.
ENOG4110YU0. LUCA.
GeneTreeiENSGT00550000074559.
HOGENOMiHOG000115735.
HOVERGENiHBG106842.
InParanoidiP28161.
KOiK00799.
OMAiRITESWA.
PhylomeDBiP28161.
TreeFamiTF353040.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR003081. GST_mu.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
PRINTSiPR01267. GSTRNSFRASEM.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P28161-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPMTLGYWNI RGLAHSIRLL LEYTDSSYEE KKYTMGDAPD YDRSQWLNEK
60 70 80 90 100
FKLGLDFPNL PYLIDGTHKI TQSNAILRYI ARKHNLCGES EKEQIREDIL
110 120 130 140 150
ENQFMDSRMQ LAKLCYDPDF EKLKPEYLQA LPEMLKLYSQ FLGKQPWFLG
160 170 180 190 200
DKITFVDFIA YDVLERNQVF EPSCLDAFPN LKDFISRFEG LEKISAYMKS
210
SRFLPRPVFT KMAVWGNK
Length:218
Mass (Da):25,745
Last modified:January 23, 2007 - v2
Checksum:i15203709D4A63789
GO
Isoform 2 (identifier: P28161-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     190-218: GLEKISAYMKSSRFLPRPVFTKMAVWGNK → HS

Note: No experimental confirmation available.
Show »
Length:191
Mass (Da):22,644
Checksum:i9267243C014F878F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2P → S in AAV38750 (Ref. 3) Curated1
Sequence conflicti9N → D in AAV38750 (Ref. 3) Curated1
Sequence conflicti51F → L in BAG61446 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_049486173S → N.Corresponds to variant rs2229050dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_045614190 – 218GLEKI…VWGNK → HS in isoform 2. CuratedAdd BLAST29

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63509 mRNA. Translation: AAA60963.1.
AK299482 mRNA. Translation: BAG61446.1.
BT019947 mRNA. Translation: AAV38750.1.
AC000031 Genomic DNA. No translation available.
AC000032 Genomic DNA. No translation available.
BC105038 mRNA. Translation: AAI05039.1.
BC105066 mRNA. Translation: AAI05067.1.
CCDSiCCDS44192.1. [P28161-2]
CCDS808.1. [P28161-1]
PIRiA39375.
RefSeqiNP_000839.1. NM_000848.3. [P28161-1]
NP_001135840.1. NM_001142368.1. [P28161-2]
UniGeneiHs.279837.

Genome annotation databases

EnsembliENST00000241337; ENSP00000241337; ENSG00000213366. [P28161-1]
ENST00000442650; ENSP00000416883; ENSG00000213366. [P28161-2]
ENST00000460717; ENSP00000435910; ENSG00000213366. [P28161-2]
GeneIDi2946.
KEGGihsa:2946.
UCSCiuc001dyj.4. human. [P28161-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63509 mRNA. Translation: AAA60963.1.
AK299482 mRNA. Translation: BAG61446.1.
BT019947 mRNA. Translation: AAV38750.1.
AC000031 Genomic DNA. No translation available.
AC000032 Genomic DNA. No translation available.
BC105038 mRNA. Translation: AAI05039.1.
BC105066 mRNA. Translation: AAI05067.1.
CCDSiCCDS44192.1. [P28161-2]
CCDS808.1. [P28161-1]
PIRiA39375.
RefSeqiNP_000839.1. NM_000848.3. [P28161-1]
NP_001135840.1. NM_001142368.1. [P28161-2]
UniGeneiHs.279837.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HNAX-ray1.85A2-218[»]
1HNBX-ray3.50A/B2-218[»]
1HNCX-ray3.00A/B/C/D2-218[»]
1XW5X-ray1.80A/B2-218[»]
1YKCX-ray2.10A/B2-218[»]
2AB6X-ray2.50A/B/C/D2-218[»]
2C4JX-ray1.35A/B/C/D2-218[»]
2GTUX-ray2.55A/B2-218[»]
3GTUX-ray2.80A/C2-218[»]
3GURX-ray2.50A/B/C/D2-218[»]
ProteinModelPortaliP28161.
SMRiP28161.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109201. 6 interactors.
IntActiP28161. 1 interactor.
STRINGi9606.ENSP00000241337.

Chemistry databases

BindingDBiP28161.
ChEMBLiCHEMBL4589.
DrugBankiDB00143. Glutathione.
SwissLipidsiSLP:000001614.

PTM databases

iPTMnetiP28161.
PhosphoSitePlusiP28161.

Polymorphism and mutation databases

BioMutaiGSTM2.
DMDMi232204.

2D gel databases

OGPiP28161.
REPRODUCTION-2DPAGEP28161.

Proteomic databases

EPDiP28161.
MaxQBiP28161.
PaxDbiP28161.
PeptideAtlasiP28161.
PRIDEiP28161.

Protocols and materials databases

DNASUi2946.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000241337; ENSP00000241337; ENSG00000213366. [P28161-1]
ENST00000442650; ENSP00000416883; ENSG00000213366. [P28161-2]
ENST00000460717; ENSP00000435910; ENSG00000213366. [P28161-2]
GeneIDi2946.
KEGGihsa:2946.
UCSCiuc001dyj.4. human. [P28161-1]

Organism-specific databases

CTDi2946.
DisGeNETi2946.
GeneCardsiGSTM2.
HGNCiHGNC:4634. GSTM2.
HPAiCAB040547.
HPA055972.
HPA055973.
MIMi138380. gene.
neXtProtiNX_P28161.
OpenTargetsiENSG00000213366.
PharmGKBiPA29023.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IN5J. Eukaryota.
ENOG4110YU0. LUCA.
GeneTreeiENSGT00550000074559.
HOGENOMiHOG000115735.
HOVERGENiHBG106842.
InParanoidiP28161.
KOiK00799.
OMAiRITESWA.
PhylomeDBiP28161.
TreeFamiTF353040.

Enzyme and pathway databases

BioCyciZFISH:HS09818-MONOMER.
BRENDAi2.5.1.18. 2681.
ReactomeiR-HSA-156590. Glutathione conjugation.
SABIO-RKP28161.

Miscellaneous databases

ChiTaRSiGSTM2. human.
EvolutionaryTraceiP28161.
GeneWikiiGSTM2.
GenomeRNAii2946.
PROiP28161.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000213366.
CleanExiHS_GSTM2.
ExpressionAtlasiP28161. baseline and differential.
GenevisibleiP28161. HS.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR003081. GST_mu.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
PRINTSiPR01267. GSTRNSFRASEM.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGSTM2_HUMAN
AccessioniPrimary (citable) accession number: P28161
Secondary accession number(s): B4DRY4
, E9PEM9, Q2M318, Q5TZY5, Q8WWE1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 174 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.