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Protein

Suppressor of hairless protein

Gene

Su(H)

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Binds directly the 5'-GTGRGAR-3' DNA consensus sequence, which is present in the regulatory region of several genes. Required for neurogenesis in imaginal disks. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some Notch protein, it acts as a transcriptional activator that activates transcription of Notch target genes. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. Required for transcription of Sim. Also functions independently of Notch pathway, in the development of the bristle sensory organ precursor cell.3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi132 – 1398By similarity
DNA bindingi266 – 27510By similarity
DNA bindingi339 – 37133By similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • asymmetric cell division Source: FlyBase
  • cell fate determination Source: FlyBase
  • crystal cell differentiation Source: FlyBase
  • hemopoiesis Source: FlyBase
  • I-kappaB phosphorylation Source: FlyBase
  • imaginal disc-derived wing vein morphogenesis Source: FlyBase
  • lateral inhibition Source: FlyBase
  • long-term memory Source: FlyBase
  • negative regulation of compound eye cone cell fate specification Source: FlyBase
  • negative regulation of transcription, DNA-templated Source: FlyBase
  • negative regulation of transcription from RNA polymerase II promoter Source: FlyBase
  • Notch signaling pathway Source: UniProtKB
  • positive regulation of G1/S transition of mitotic cell cycle Source: FlyBase
  • positive regulation of transcription, DNA-templated Source: FlyBase
  • positive regulation of transcription from RNA polymerase II promoter Source: FlyBase
  • sensory organ precursor cell fate determination Source: UniProtKB
  • wing disc dorsal/ventral pattern formation Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein, Repressor

Keywords - Biological processi

Notch signaling pathway, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

SignaLinkiP28159.

Names & Taxonomyi

Protein namesi
Recommended name:
Suppressor of hairless protein
Alternative name(s):
J kappa-recombination signal-binding protein
RBP-J kappa
Gene namesi
Name:Su(H)
Synonyms:dRBP-JK
ORF Names:CG3497
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0004837. Su(H).

Subcellular locationi

  • Nucleus 1 Publication
  • Cytoplasm 1 Publication

  • Note: In imaginal disk, at the onset of differentiation of socket cell, it is also present in the cytoplasm.

GO - Cellular componenti

  • cytoplasm Source: FlyBase
  • nucleus Source: FlyBase
  • polytene chromosome Source: FlyBase
  • protein complex Source: FlyBase
  • transcriptional repressor complex Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi315 – 3151Y → F: No effect. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 594594Suppressor of hairless proteinPRO_0000208572Add
BLAST

Proteomic databases

PaxDbiP28159.
PRIDEiP28159.

Expressioni

Gene expression databases

BgeeiFBgn0004837.
ExpressionAtlasiP28159. differential.
GenevisibleiP28159. DM.

Interactioni

Subunit structurei

Interacts with activated cleaved Notch. Interacts with Hairless, this interaction preventing its DNA-binding activity. Interacts with insv (via BEN domain).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
insvQ8SYK53EBI-92180,EBI-192407
NP072074EBI-92180,EBI-103438
Notch1Q017053EBI-92180,EBI-1392707From a different organism.

Protein-protein interaction databases

BioGridi60899. 39 interactions.
DIPiDIP-177N.
IntActiP28159. 11 interactions.
MINTiMINT-335544.
STRINGi7227.FBpp0080261.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5E24X-ray2.14E/F99-522[»]
ProteinModelPortaliP28159.
SMRiP28159. Positions 103-522.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini429 – 51991IPT/TIGAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi35 – 428Gln-rich
Compositional biasi58 – 8831Gln-richAdd
BLAST
Compositional biasi541 – 55818Asn-richAdd
BLAST
Compositional biasi569 – 5768Gln-rich

Sequence similaritiesi

Belongs to the Su(H) family.Curated
Contains 1 IPT/TIG domain.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3743. Eukaryota.
ENOG410XV7K. LUCA.
InParanoidiP28159.
KOiK06053.
OMAiVIVILHA.
OrthoDBiEOG091G057S.
PhylomeDBiP28159.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1450. 1 hit.
InterProiIPR015350. Beta-trefoil_DNA-bd_dom.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR015351. LAG1_DNA-bd.
IPR008967. p53-like_TF_DNA-bd.
[Graphical view]
PfamiPF09270. BTD. 1 hit.
PF09271. LAG1-DNAbind. 1 hit.
[Graphical view]
SMARTiSM01268. BTD. 1 hit.
SM01267. LAG1_DNAbind. 1 hit.
[Graphical view]
SUPFAMiSSF110217. SSF110217. 1 hit.
SSF49417. SSF49417. 1 hit.
SSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

P28159-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSYSQFNLN AAAPPAIAYE TTVVNPNGSP LDPHQQQQQQ SQDMPHFGLP
60 70 80 90 100
GPQPPSSQQQ QQQLQVHHQQ QQQQQQQQQQ QQHQQQMQMS LLPGPYRPHI
110 120 130 140 150
EEKKLTRDAM EKYMRERNDM VIVILHAKVA QKSYGNEKRF FCPPPCIYLF
160 170 180 190 200
GSGWRRRYEE MLQQGEGEQG AQLCAFIGIG SSDQDMQQLD LNGKQYCAAK
210 220 230 240 250
TLFISDSDKR KHFMLSVKMF YGNGHDIGVF NSKRIKVISK PSKKKQSLKN
260 270 280 290 300
ADLCIASGTN VALFNRLRSQ TVSTRYLHVE NGHFHASSTQ WGAFTIHLLD
310 320 330 340 350
DNESESEEFQ VRDGYIHYGA TVKLVCSVTG MALPRLIIRK VDKQMALLEA
360 370 380 390 400
DDPVSQLHKC AFYMKDTDRM YLCLSQEKII QFQATPCPKE PNKEMINDGA
410 420 430 440 450
CWTIISTDKA EYQFYEGMGP VASPVTPVPI VNSLNLNGGG DVAMLELSGD
460 470 480 490 500
NFTPHLQVWF GDVEAETMYR CTETLLCVVP EISQFRGEWL WVRQPTQVPI
510 520 530 540 550
SLVRNDGIIY ATGLTFTYTP EPGPRPHCNT QAEDVMRARQ NNNNNNITSI
560 570 580 590
SNNNNSNNAG SPAAGGGLQQ QQQQHQALPS ISEVQWNSHG SGLS
Length:594
Mass (Da):66,924
Last modified:July 1, 1993 - v1
Checksum:i145144F336CD8F4C
GO

Sequence cautioni

The sequence AAA06211 differs from that shown. Reason: Frameshift at position 6. Curated
The sequence CAA41282 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti116 – 1172ER → DG in AAA06211 (PubMed:1744127).Curated
Sequence conflicti116 – 1172ER → DG in CAA41282 (PubMed:1744127).Curated
Sequence conflicti492 – 4921V → A in AAA06211 (PubMed:1744127).Curated
Sequence conflicti492 – 4921V → A in CAA41282 (PubMed:1744127).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti80 – 823Missing in strain: LAMTO 3, LAMTO 4, LAMTO 12, LAMTO 18, LAMTO 19, LAMTO 21, LAMTO 31, LAMTO 35, LAMTO 101 and LAMTO 111.
Natural varianti81 – 822Missing in strain: LAMTO 13, LAMTO 37 and LAMTO 134.
Natural varianti82 – 821Missing in strain: LAMTO 124.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S69213 Genomic DNA. Translation: AAA06211.1. Frameshift.
X58393 mRNA. Translation: CAA41282.1. Different initiation.
M94383 mRNA. Translation: AAA28919.1.
AE014134 Genomic DNA. Translation: AAF53434.1.
AY069091 mRNA. Translation: AAL39236.1.
AF088255 Genomic DNA. Translation: AAD39698.1.
AF088256 Genomic DNA. Translation: AAD39699.1.
AF088257 Genomic DNA. Translation: AAD39700.1.
AF088258 Genomic DNA. Translation: AAD39701.1.
AF088259 Genomic DNA. Translation: AAD39702.1.
AF088260 Genomic DNA. Translation: AAD39703.1.
AF088261 Genomic DNA. Translation: AAD39704.1.
AF088262 Genomic DNA. Translation: AAD39705.1.
AF088263 Genomic DNA. Translation: AAD39706.1.
AF088264 Genomic DNA. Translation: AAD39707.1.
AF088265 Genomic DNA. Translation: AAD39708.1.
AF088266 Genomic DNA. Translation: AAD39709.1.
AF088267 Genomic DNA. Translation: AAD39710.1.
AF088268 Genomic DNA. Translation: AAD39711.1.
AF088269 Genomic DNA. Translation: AAD39712.1.
AF088270 Genomic DNA. Translation: AAD39713.1.
AF088271 Genomic DNA. Translation: AAD39714.1.
AF088272 Genomic DNA. Translation: AAD39715.1.
AF088273 Genomic DNA. Translation: AAD39716.1.
AF088274 Genomic DNA. Translation: AAD39717.1.
PIRiA41585.
A42770.
RefSeqiNP_001285948.1. NM_001299019.1.
NP_476868.1. NM_057520.4.
UniGeneiDm.2288.

Genome annotation databases

EnsemblMetazoaiFBtr0080700; FBpp0080261; FBgn0004837.
FBtr0346621; FBpp0312201; FBgn0004837.
GeneIDi34881.
KEGGidme:Dmel_CG3497.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S69213 Genomic DNA. Translation: AAA06211.1. Frameshift.
X58393 mRNA. Translation: CAA41282.1. Different initiation.
M94383 mRNA. Translation: AAA28919.1.
AE014134 Genomic DNA. Translation: AAF53434.1.
AY069091 mRNA. Translation: AAL39236.1.
AF088255 Genomic DNA. Translation: AAD39698.1.
AF088256 Genomic DNA. Translation: AAD39699.1.
AF088257 Genomic DNA. Translation: AAD39700.1.
AF088258 Genomic DNA. Translation: AAD39701.1.
AF088259 Genomic DNA. Translation: AAD39702.1.
AF088260 Genomic DNA. Translation: AAD39703.1.
AF088261 Genomic DNA. Translation: AAD39704.1.
AF088262 Genomic DNA. Translation: AAD39705.1.
AF088263 Genomic DNA. Translation: AAD39706.1.
AF088264 Genomic DNA. Translation: AAD39707.1.
AF088265 Genomic DNA. Translation: AAD39708.1.
AF088266 Genomic DNA. Translation: AAD39709.1.
AF088267 Genomic DNA. Translation: AAD39710.1.
AF088268 Genomic DNA. Translation: AAD39711.1.
AF088269 Genomic DNA. Translation: AAD39712.1.
AF088270 Genomic DNA. Translation: AAD39713.1.
AF088271 Genomic DNA. Translation: AAD39714.1.
AF088272 Genomic DNA. Translation: AAD39715.1.
AF088273 Genomic DNA. Translation: AAD39716.1.
AF088274 Genomic DNA. Translation: AAD39717.1.
PIRiA41585.
A42770.
RefSeqiNP_001285948.1. NM_001299019.1.
NP_476868.1. NM_057520.4.
UniGeneiDm.2288.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5E24X-ray2.14E/F99-522[»]
ProteinModelPortaliP28159.
SMRiP28159. Positions 103-522.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi60899. 39 interactions.
DIPiDIP-177N.
IntActiP28159. 11 interactions.
MINTiMINT-335544.
STRINGi7227.FBpp0080261.

Proteomic databases

PaxDbiP28159.
PRIDEiP28159.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0080700; FBpp0080261; FBgn0004837.
FBtr0346621; FBpp0312201; FBgn0004837.
GeneIDi34881.
KEGGidme:Dmel_CG3497.

Organism-specific databases

CTDi34881.
FlyBaseiFBgn0004837. Su(H).

Phylogenomic databases

eggNOGiKOG3743. Eukaryota.
ENOG410XV7K. LUCA.
InParanoidiP28159.
KOiK06053.
OMAiVIVILHA.
OrthoDBiEOG091G057S.
PhylomeDBiP28159.

Enzyme and pathway databases

SignaLinkiP28159.

Miscellaneous databases

GenomeRNAii34881.
PROiP28159.

Gene expression databases

BgeeiFBgn0004837.
ExpressionAtlasiP28159. differential.
GenevisibleiP28159. DM.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1450. 1 hit.
InterProiIPR015350. Beta-trefoil_DNA-bd_dom.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR015351. LAG1_DNA-bd.
IPR008967. p53-like_TF_DNA-bd.
[Graphical view]
PfamiPF09270. BTD. 1 hit.
PF09271. LAG1-DNAbind. 1 hit.
[Graphical view]
SMARTiSM01268. BTD. 1 hit.
SM01267. LAG1_DNAbind. 1 hit.
[Graphical view]
SUPFAMiSSF110217. SSF110217. 1 hit.
SSF49417. SSF49417. 1 hit.
SSF81296. SSF81296. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSUH_DROME
AccessioniPrimary (citable) accession number: P28159
Secondary accession number(s): Q3S1M8
, Q9TVK8, Q9TVY7, Q9TW34, Q9U8F9, Q9V446
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: September 7, 2016
This is version 163 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Caution

Despite some similarity with the "phage" integrase family, PubMed:7958912 showed that it has no recombinase activity.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.