Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

TATA-box-binding protein 2

Gene

TBP2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

General transcription factor (GTF) that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II (By similarity). Interacts with TFIIB1 and is required for activated transcription and possibly basal transcription (PubMed:10634912). May act as GTF of RNA polymerase I-dependent transcription and rRNA synthesis. Forms a ternary complex with PBRP1 and the rDNA promoter region (PubMed:18668124).By similarity2 Publications

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • TBP-class protein binding Source: TAIR

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-ATH-674695. RNA Polymerase II Pre-transcription Events.
R-ATH-73776. RNA Polymerase II Promoter Escape.
R-ATH-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-ATH-75953. RNA Polymerase II Transcription Initiation.
R-ATH-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.

Names & Taxonomyi

Protein namesi
Recommended name:
TATA-box-binding protein 2
Alternative name(s):
TATA sequence-binding protein 2
Short name:
TBP-2
TATA-binding factor 2
TATA-box factor 2
Transcription initiation factor TFIID TBP-2 subunit
Gene namesi
Name:TBP2
Ordered Locus Names:At1g55520
ORF Names:T5A14.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G55520.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi144 – 1441E → R: Unable to bind to TFIIB1. 1 Publication
Mutagenesisi146 – 1461E → R: Unable to bind to TFIIB1. 1 Publication
Mutagenesisi197 – 1971K → E: No effect on the binding to TFIIB1. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 200199TATA-box-binding protein 2PRO_0000153977Add
BLAST

Proteomic databases

PaxDbiP28148.
PRIDEiP28148.

Expressioni

Gene expression databases

ExpressionAtlasiP28148. baseline and differential.
GenevisibleiP28148. AT.

Interactioni

Subunit structurei

Belongs to the TFIID complex together with the TBP-associated factors (TAFs). Binds DNA as monomer. Interacts with TAF1 (via N-terminus) (PubMed:17340043). Interacts with TFIIB1 (PubMed:10634912). Interacts with PTF2 (PubMed:23713077). Interacts with HAT5/ATHB-1 and ATHB-7 (PubMed:24531799).4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
HSFA1AP411512EBI-1544976,EBI-1544927

GO - Molecular functioni

  • TBP-class protein binding Source: TAIR

Protein-protein interaction databases

BioGridi27224. 9 interactions.
IntActiP28148. 3 interactions.
STRINGi3702.AT1G55520.1.

Structurei

3D structure databases

ProteinModelPortaliP28148.
SMRiP28148. Positions 12-197.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati25 – 101771Add
BLAST
Repeati115 – 192782Add
BLAST

Sequence similaritiesi

Belongs to the TBP family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3302. Eukaryota.
COG2101. LUCA.
HOGENOMiHOG000105162.
InParanoidiP28148.
KOiK03120.
OMAiLIYRMKL.
PhylomeDBiP28148.

Family and domain databases

Gene3Di3.30.310.10. 2 hits.
HAMAPiMF_00408. TATA_bind_prot_arch.
InterProiIPR012295. Beta2_adaptin/TBP_C_dom.
IPR000814. TBP.
IPR030491. TBP_CS.
[Graphical view]
PANTHERiPTHR10126. PTHR10126. 1 hit.
PfamiPF00352. TBP. 2 hits.
[Graphical view]
PRINTSiPR00686. TIFACTORIID.
PROSITEiPS00351. TFIID. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28148-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADQGTEGSQ PVDLTKHPSG IVPTLQNIVS TVNLDCKLDL KAIALQARNA
60 70 80 90 100
EYNPKRFAAV IMRIREPKTT ALIFASGKMV CTGAKSEHLS KLAARKYARI
110 120 130 140 150
VQKLGFPAKF KDFKIQNIVG SCDVKFPIRL EGLAYSHSAF SSYEPELFPG
160 170 180 190 200
LIYRMKLPKI VLLIFVSGKI VITGAKMREE TYTAFENIYP VLREFRKVQQ
Length:200
Mass (Da):22,396
Last modified:December 1, 1992 - v1
Checksum:iCFB9596EF8D9CEDD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54995 mRNA. Translation: CAA38742.1.
AY463626 mRNA. Translation: AAR28028.1.
AC005223 Genomic DNA. Translation: AAD10645.1.
CP002684 Genomic DNA. Translation: AEE33257.1.
CP002684 Genomic DNA. Translation: AEE33258.1.
BT006488 mRNA. Translation: AAP21296.1.
AK227470 mRNA. Translation: BAE99472.1.
AB493506 mRNA. Translation: BAH30344.1.
PIRiS10945.
RefSeqiNP_175948.1. NM_104427.3.
NP_849812.1. NM_179481.2.
UniGeneiAt.192.

Genome annotation databases

EnsemblPlantsiAT1G55520.1; AT1G55520.1; AT1G55520.
AT1G55520.2; AT1G55520.2; AT1G55520.
GeneIDi841999.
GrameneiAT1G55520.1; AT1G55520.1; AT1G55520.
AT1G55520.2; AT1G55520.2; AT1G55520.
KEGGiath:AT1G55520.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54995 mRNA. Translation: CAA38742.1.
AY463626 mRNA. Translation: AAR28028.1.
AC005223 Genomic DNA. Translation: AAD10645.1.
CP002684 Genomic DNA. Translation: AEE33257.1.
CP002684 Genomic DNA. Translation: AEE33258.1.
BT006488 mRNA. Translation: AAP21296.1.
AK227470 mRNA. Translation: BAE99472.1.
AB493506 mRNA. Translation: BAH30344.1.
PIRiS10945.
RefSeqiNP_175948.1. NM_104427.3.
NP_849812.1. NM_179481.2.
UniGeneiAt.192.

3D structure databases

ProteinModelPortaliP28148.
SMRiP28148. Positions 12-197.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi27224. 9 interactions.
IntActiP28148. 3 interactions.
STRINGi3702.AT1G55520.1.

Proteomic databases

PaxDbiP28148.
PRIDEiP28148.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G55520.1; AT1G55520.1; AT1G55520.
AT1G55520.2; AT1G55520.2; AT1G55520.
GeneIDi841999.
GrameneiAT1G55520.1; AT1G55520.1; AT1G55520.
AT1G55520.2; AT1G55520.2; AT1G55520.
KEGGiath:AT1G55520.

Organism-specific databases

TAIRiAT1G55520.

Phylogenomic databases

eggNOGiKOG3302. Eukaryota.
COG2101. LUCA.
HOGENOMiHOG000105162.
InParanoidiP28148.
KOiK03120.
OMAiLIYRMKL.
PhylomeDBiP28148.

Enzyme and pathway databases

ReactomeiR-ATH-674695. RNA Polymerase II Pre-transcription Events.
R-ATH-73776. RNA Polymerase II Promoter Escape.
R-ATH-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-ATH-75953. RNA Polymerase II Transcription Initiation.
R-ATH-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.

Miscellaneous databases

PROiP28148.

Gene expression databases

ExpressionAtlasiP28148. baseline and differential.
GenevisibleiP28148. AT.

Family and domain databases

Gene3Di3.30.310.10. 2 hits.
HAMAPiMF_00408. TATA_bind_prot_arch.
InterProiIPR012295. Beta2_adaptin/TBP_C_dom.
IPR000814. TBP.
IPR030491. TBP_CS.
[Graphical view]
PANTHERiPTHR10126. PTHR10126. 1 hit.
PfamiPF00352. TBP. 2 hits.
[Graphical view]
PRINTSiPR00686. TIFACTORIID.
PROSITEiPS00351. TFIID. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Arabidopsis thaliana contains two genes for TFIID."
    Gasch A., Hoffmann A., Horikoshi M., Roeder R.G., Chua N.-H.
    Nature 346:390-394(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Binary protein-protein interactions of the Arabidopsis thaliana general transcription factor IId."
    Lawit S.J., Gurley W.B.
    Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  3. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  7. "ORF cloning and analysis of Arabidopsis transcription factor genes."
    Fujita M., Mizukado S., Seki M., Shinozaki K., Mitsuda N., Takiguchi Y., Takagi M.
    Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  8. "Role of the TATA binding protein-transcription factor IIB interaction in supporting basal and activated transcription in plant cells."
    Pan S., Czarnecka-Verner E., Gurley W.B.
    Plant Cell 12:125-136(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH TFIIB1, MUTAGENESIS OF GLU-144; GLU-146 AND LYS-197.
  9. Cited for: INTERACTION WITH TAF1.
  10. "The plant-specific TFIIB-related protein, pBrp, is a general transcription factor for RNA polymerase I."
    Imamura S., Hanaoka M., Tanaka K.
    EMBO J. 27:2317-2327(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "Pollen-expressed transcription factor 2 encodes a novel plant-specific TFIIB-related protein that is required for pollen germination and embryogenesis in Arabidopsis."
    Niu Q.K., Liang Y., Zhou J.J., Dou X.Y., Gao S.C., Chen L.Q., Zhang X.Q., Ye D.
    Mol. Plant 6:1091-1108(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PTF2.
  12. "Plant homeodomain-leucine zipper I transcription factors exhibit different functional AHA motifs that selectively interact with TBP or/and TFIIB."
    Capella M., Re D.A., Arce A.L., Chan R.L.
    Plant Cell Rep. 33:955-967(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HAT5/ATHB-1 AND ATHB-7.

Entry informationi

Entry nameiTBP2_ARATH
AccessioniPrimary (citable) accession number: P28148
Secondary accession number(s): Q53YT7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: July 6, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.