Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

TATA-box-binding protein 1

Gene

TBP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-ATH-674695. RNA Polymerase II Pre-transcription Events.
R-ATH-73776. RNA Polymerase II Promoter Escape.
R-ATH-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-ATH-75953. RNA Polymerase II Transcription Initiation.
R-ATH-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.

Names & Taxonomyi

Protein namesi
Recommended name:
TATA-box-binding protein 1
Alternative name(s):
TATA sequence-binding protein 1
Short name:
TBP-1
TATA-binding factor 1
TATA-box factor 1
Transcription initiation factor TFIID TBP-1 subunit
Gene namesi
Name:TBP1
Ordered Locus Names:At3g13445
ORF Names:MRP15.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G13445.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001539762 – 200TATA-box-binding protein 1Add BLAST199

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylthreonineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP28147.

Expressioni

Gene expression databases

ExpressionAtlasiP28147. baseline and differential.
GenevisibleiP28147. AT.

Interactioni

Subunit structurei

Belongs to the TFIID complex together with the TBP-associated factors (TAFs). Binds DNA as monomer. Interacts with TAF1 (via N-terminus) (PubMed:17340043). Interacts with MEE12/CCG1 (PubMed:26462908).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
HSFA1AP411513EBI-1247453,EBI-1544927

Protein-protein interaction databases

BioGridi5880. 2 interactors.
IntActiP28147. 3 interactors.
MINTiMINT-1508178.
STRINGi3702.AT3G13445.1.

Structurei

Secondary structure

1200
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni14 – 16Combined sources3
Beta strandi24 – 33Combined sources10
Helixi40 – 46Combined sources7
Beta strandi48 – 52Combined sources5
Turni54 – 56Combined sources3
Beta strandi58 – 64Combined sources7
Turni65 – 68Combined sources4
Beta strandi69 – 73Combined sources5
Beta strandi77 – 86Combined sources10
Helixi87 – 103Combined sources17
Beta strandi111 – 123Combined sources13
Helixi130 – 136Combined sources7
Turni137 – 140Combined sources4
Beta strandi141 – 143Combined sources3
Turni145 – 147Combined sources3
Beta strandi149 – 155Combined sources7
Turni156 – 159Combined sources4
Beta strandi160 – 164Combined sources5
Beta strandi168 – 177Combined sources10
Helixi178 – 194Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1QN3X-ray1.95A/B1-200[»]
1QN4X-ray1.86A/B1-200[»]
1QN5X-ray1.93A/B1-200[»]
1QN6X-ray2.10A/B1-200[»]
1QN7X-ray2.30A/B1-200[»]
1QN8X-ray2.10A/B1-200[»]
1QN9X-ray1.90A/B1-200[»]
1QNAX-ray1.80A/B1-200[»]
1QNBX-ray2.23A/B1-200[»]
1QNCX-ray2.30A/B1-200[»]
1QNEX-ray1.90A/B1-200[»]
1VOKX-ray2.10A/B1-200[»]
1VOLX-ray2.70B1-200[»]
1VTLX-ray2.25E/F13-198[»]
1VTOX-ray1.90A/B11-200[»]
ProteinModelPortaliP28147.
SMRiP28147.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP28147.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati25 – 1011Add BLAST77
Repeati115 – 1922Add BLAST78

Sequence similaritiesi

Belongs to the TBP family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3302. Eukaryota.
COG2101. LUCA.
HOGENOMiHOG000105162.
InParanoidiP28147.
KOiK03120.
OMAiKEGIMAM.
OrthoDBiEOG09360MFO.
PhylomeDBiP28147.

Family and domain databases

CDDicd04516. TBP_eukaryotes. 1 hit.
Gene3Di3.30.310.10. 2 hits.
HAMAPiMF_00408. TATA_bind_prot_arch. 1 hit.
InterProiIPR012295. Beta2_adaptin/TBP_C_dom.
IPR000814. TBP.
IPR030491. TBP_CS.
IPR033710. TBP_eukaryotic.
[Graphical view]
PfamiPF00352. TBP. 2 hits.
[Graphical view]
PRINTSiPR00686. TIFACTORIID.
PROSITEiPS00351. TFIID. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: P28147-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTDQGLEGSN PVDLSKHPSG IVPTLQNIVS TVNLDCKLDL KAIALQARNA
60 70 80 90 100
EYNPKRFAAV IMRIREPKTT ALIFASGKMV CTGAKSEDFS KMAARKYARI
110 120 130 140 150
VQKLGFPAKF KDFKIQNIVG SCDVKFPIRL EGLAYSHAAF SSYEPELFPG
160 170 180 190 200
LIYRMKVPKI VLLIFVSGKI VITGAKMRDE TYKAFENIYP VLSEFRKIQQ
Length:200
Mass (Da):22,368
Last modified:December 1, 1992 - v1
Checksum:i9E15DC2308818919
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54996 mRNA. Translation: CAA38743.1.
AY463625 mRNA. Translation: AAR28027.1.
AP000603 Genomic DNA. Translation: BAB01751.1.
CP002686 Genomic DNA. Translation: AEE75355.1.
AF324696 mRNA. Translation: AAG40047.1.
AF327429 mRNA. Translation: AAG42019.1.
AF349523 mRNA. Translation: AAK15570.1.
AY054608 mRNA. Translation: AAK96799.1.
AY072455 mRNA. Translation: AAL66870.1.
AY084881 mRNA. Translation: AAM61444.1.
AB493611 mRNA. Translation: BAH30449.1.
PIRiS10946.
RefSeqiNP_187953.1. NM_112190.5. [P28147-1]
UniGeneiAt.20074.
At.24512.

Genome annotation databases

EnsemblPlantsiAT3G13445.1; AT3G13445.1; AT3G13445. [P28147-1]
GeneIDi820546.
GrameneiAT3G13445.1; AT3G13445.1; AT3G13445.
KEGGiath:AT3G13445.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54996 mRNA. Translation: CAA38743.1.
AY463625 mRNA. Translation: AAR28027.1.
AP000603 Genomic DNA. Translation: BAB01751.1.
CP002686 Genomic DNA. Translation: AEE75355.1.
AF324696 mRNA. Translation: AAG40047.1.
AF327429 mRNA. Translation: AAG42019.1.
AF349523 mRNA. Translation: AAK15570.1.
AY054608 mRNA. Translation: AAK96799.1.
AY072455 mRNA. Translation: AAL66870.1.
AY084881 mRNA. Translation: AAM61444.1.
AB493611 mRNA. Translation: BAH30449.1.
PIRiS10946.
RefSeqiNP_187953.1. NM_112190.5. [P28147-1]
UniGeneiAt.20074.
At.24512.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1QN3X-ray1.95A/B1-200[»]
1QN4X-ray1.86A/B1-200[»]
1QN5X-ray1.93A/B1-200[»]
1QN6X-ray2.10A/B1-200[»]
1QN7X-ray2.30A/B1-200[»]
1QN8X-ray2.10A/B1-200[»]
1QN9X-ray1.90A/B1-200[»]
1QNAX-ray1.80A/B1-200[»]
1QNBX-ray2.23A/B1-200[»]
1QNCX-ray2.30A/B1-200[»]
1QNEX-ray1.90A/B1-200[»]
1VOKX-ray2.10A/B1-200[»]
1VOLX-ray2.70B1-200[»]
1VTLX-ray2.25E/F13-198[»]
1VTOX-ray1.90A/B11-200[»]
ProteinModelPortaliP28147.
SMRiP28147.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi5880. 2 interactors.
IntActiP28147. 3 interactors.
MINTiMINT-1508178.
STRINGi3702.AT3G13445.1.

Proteomic databases

PaxDbiP28147.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G13445.1; AT3G13445.1; AT3G13445. [P28147-1]
GeneIDi820546.
GrameneiAT3G13445.1; AT3G13445.1; AT3G13445.
KEGGiath:AT3G13445.

Organism-specific databases

TAIRiAT3G13445.

Phylogenomic databases

eggNOGiKOG3302. Eukaryota.
COG2101. LUCA.
HOGENOMiHOG000105162.
InParanoidiP28147.
KOiK03120.
OMAiKEGIMAM.
OrthoDBiEOG09360MFO.
PhylomeDBiP28147.

Enzyme and pathway databases

ReactomeiR-ATH-674695. RNA Polymerase II Pre-transcription Events.
R-ATH-73776. RNA Polymerase II Promoter Escape.
R-ATH-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-ATH-75953. RNA Polymerase II Transcription Initiation.
R-ATH-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.

Miscellaneous databases

EvolutionaryTraceiP28147.
PROiP28147.

Gene expression databases

ExpressionAtlasiP28147. baseline and differential.
GenevisibleiP28147. AT.

Family and domain databases

CDDicd04516. TBP_eukaryotes. 1 hit.
Gene3Di3.30.310.10. 2 hits.
HAMAPiMF_00408. TATA_bind_prot_arch. 1 hit.
InterProiIPR012295. Beta2_adaptin/TBP_C_dom.
IPR000814. TBP.
IPR030491. TBP_CS.
IPR033710. TBP_eukaryotic.
[Graphical view]
PfamiPF00352. TBP. 2 hits.
[Graphical view]
PRINTSiPR00686. TIFACTORIID.
PROSITEiPS00351. TFIID. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTBP1_ARATH
AccessioniPrimary (citable) accession number: P28147
Secondary accession number(s): Q53YT8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: November 2, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.