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Protein

TATA-box-binding protein 1

Gene

TBP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-ATH-674695. RNA Polymerase II Pre-transcription Events.
R-ATH-73776. RNA Polymerase II Promoter Escape.
R-ATH-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-ATH-75953. RNA Polymerase II Transcription Initiation.
R-ATH-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.

Names & Taxonomyi

Protein namesi
Recommended name:
TATA-box-binding protein 1
Alternative name(s):
TATA sequence-binding protein 1
Short name:
TBP-1
TATA-binding factor 1
TATA-box factor 1
Transcription initiation factor TFIID TBP-1 subunit
Gene namesi
Name:TBP1
Ordered Locus Names:At3g13445
ORF Names:MRP15.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G13445.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 200199TATA-box-binding protein 1PRO_0000153976Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylthreonineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP28147.
PRIDEiP28147.

Expressioni

Gene expression databases

ExpressionAtlasiP28147. baseline and differential.
GenevisibleiP28147. AT.

Interactioni

Subunit structurei

Belongs to the TFIID complex together with the TBP-associated factors (TAFs). Binds DNA as monomer. Interacts with TAF1 (via N-terminus) (PubMed:17340043). Interacts with MEE12/CCG1 (PubMed:26462908).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
HSFA1AP411513EBI-1247453,EBI-1544927

Protein-protein interaction databases

BioGridi5880. 2 interactions.
IntActiP28147. 3 interactions.
MINTiMINT-1508178.
STRINGi3702.AT3G13445.1.

Structurei

Secondary structure

1
200
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni14 – 163Combined sources
Beta strandi24 – 3310Combined sources
Helixi40 – 467Combined sources
Beta strandi48 – 525Combined sources
Turni54 – 563Combined sources
Beta strandi58 – 647Combined sources
Turni65 – 684Combined sources
Beta strandi69 – 735Combined sources
Beta strandi77 – 8610Combined sources
Helixi87 – 10317Combined sources
Beta strandi111 – 12313Combined sources
Helixi130 – 1367Combined sources
Turni137 – 1404Combined sources
Beta strandi141 – 1433Combined sources
Turni145 – 1473Combined sources
Beta strandi149 – 1557Combined sources
Turni156 – 1594Combined sources
Beta strandi160 – 1645Combined sources
Beta strandi168 – 17710Combined sources
Helixi178 – 19417Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1QN3X-ray1.95A/B1-200[»]
1QN4X-ray1.86A/B1-200[»]
1QN5X-ray1.93A/B1-200[»]
1QN6X-ray2.10A/B1-200[»]
1QN7X-ray2.30A/B1-200[»]
1QN8X-ray2.10A/B1-200[»]
1QN9X-ray1.90A/B1-200[»]
1QNAX-ray1.80A/B1-200[»]
1QNBX-ray2.23A/B1-200[»]
1QNCX-ray2.30A/B1-200[»]
1QNEX-ray1.90A/B1-200[»]
1VOKX-ray2.10A/B1-200[»]
1VOLX-ray2.70B1-200[»]
1VTLX-ray2.25E/F13-198[»]
1VTOX-ray1.90A/B11-200[»]
ProteinModelPortaliP28147.
SMRiP28147. Positions 12-197.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP28147.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati25 – 101771Add
BLAST
Repeati115 – 192782Add
BLAST

Sequence similaritiesi

Belongs to the TBP family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3302. Eukaryota.
COG2101. LUCA.
HOGENOMiHOG000105162.
InParanoidiP28147.
KOiK03120.
OMAiMDCLEAI.
PhylomeDBiP28147.

Family and domain databases

Gene3Di3.30.310.10. 2 hits.
HAMAPiMF_00408. TATA_bind_prot_arch.
InterProiIPR012295. Beta2_adaptin/TBP_C_dom.
IPR000814. TBP.
IPR030491. TBP_CS.
[Graphical view]
PfamiPF00352. TBP. 2 hits.
[Graphical view]
PRINTSiPR00686. TIFACTORIID.
PROSITEiPS00351. TFIID. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: P28147-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTDQGLEGSN PVDLSKHPSG IVPTLQNIVS TVNLDCKLDL KAIALQARNA
60 70 80 90 100
EYNPKRFAAV IMRIREPKTT ALIFASGKMV CTGAKSEDFS KMAARKYARI
110 120 130 140 150
VQKLGFPAKF KDFKIQNIVG SCDVKFPIRL EGLAYSHAAF SSYEPELFPG
160 170 180 190 200
LIYRMKVPKI VLLIFVSGKI VITGAKMRDE TYKAFENIYP VLSEFRKIQQ
Length:200
Mass (Da):22,368
Last modified:December 1, 1992 - v1
Checksum:i9E15DC2308818919
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54996 mRNA. Translation: CAA38743.1.
AY463625 mRNA. Translation: AAR28027.1.
AP000603 Genomic DNA. Translation: BAB01751.1.
CP002686 Genomic DNA. Translation: AEE75355.1.
AF324696 mRNA. Translation: AAG40047.1.
AF327429 mRNA. Translation: AAG42019.1.
AF349523 mRNA. Translation: AAK15570.1.
AY054608 mRNA. Translation: AAK96799.1.
AY072455 mRNA. Translation: AAL66870.1.
AY084881 mRNA. Translation: AAM61444.1.
AB493611 mRNA. Translation: BAH30449.1.
PIRiS10946.
RefSeqiNP_187953.1. NM_112190.5. [P28147-1]
UniGeneiAt.20074.
At.24512.

Genome annotation databases

EnsemblPlantsiAT3G13445.1; AT3G13445.1; AT3G13445. [P28147-1]
GeneIDi820546.
KEGGiath:AT3G13445.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54996 mRNA. Translation: CAA38743.1.
AY463625 mRNA. Translation: AAR28027.1.
AP000603 Genomic DNA. Translation: BAB01751.1.
CP002686 Genomic DNA. Translation: AEE75355.1.
AF324696 mRNA. Translation: AAG40047.1.
AF327429 mRNA. Translation: AAG42019.1.
AF349523 mRNA. Translation: AAK15570.1.
AY054608 mRNA. Translation: AAK96799.1.
AY072455 mRNA. Translation: AAL66870.1.
AY084881 mRNA. Translation: AAM61444.1.
AB493611 mRNA. Translation: BAH30449.1.
PIRiS10946.
RefSeqiNP_187953.1. NM_112190.5. [P28147-1]
UniGeneiAt.20074.
At.24512.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1QN3X-ray1.95A/B1-200[»]
1QN4X-ray1.86A/B1-200[»]
1QN5X-ray1.93A/B1-200[»]
1QN6X-ray2.10A/B1-200[»]
1QN7X-ray2.30A/B1-200[»]
1QN8X-ray2.10A/B1-200[»]
1QN9X-ray1.90A/B1-200[»]
1QNAX-ray1.80A/B1-200[»]
1QNBX-ray2.23A/B1-200[»]
1QNCX-ray2.30A/B1-200[»]
1QNEX-ray1.90A/B1-200[»]
1VOKX-ray2.10A/B1-200[»]
1VOLX-ray2.70B1-200[»]
1VTLX-ray2.25E/F13-198[»]
1VTOX-ray1.90A/B11-200[»]
ProteinModelPortaliP28147.
SMRiP28147. Positions 12-197.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi5880. 2 interactions.
IntActiP28147. 3 interactions.
MINTiMINT-1508178.
STRINGi3702.AT3G13445.1.

Proteomic databases

PaxDbiP28147.
PRIDEiP28147.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G13445.1; AT3G13445.1; AT3G13445. [P28147-1]
GeneIDi820546.
KEGGiath:AT3G13445.

Organism-specific databases

TAIRiAT3G13445.

Phylogenomic databases

eggNOGiKOG3302. Eukaryota.
COG2101. LUCA.
HOGENOMiHOG000105162.
InParanoidiP28147.
KOiK03120.
OMAiMDCLEAI.
PhylomeDBiP28147.

Enzyme and pathway databases

ReactomeiR-ATH-674695. RNA Polymerase II Pre-transcription Events.
R-ATH-73776. RNA Polymerase II Promoter Escape.
R-ATH-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-ATH-75953. RNA Polymerase II Transcription Initiation.
R-ATH-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.

Miscellaneous databases

EvolutionaryTraceiP28147.
PROiP28147.

Gene expression databases

ExpressionAtlasiP28147. baseline and differential.
GenevisibleiP28147. AT.

Family and domain databases

Gene3Di3.30.310.10. 2 hits.
HAMAPiMF_00408. TATA_bind_prot_arch.
InterProiIPR012295. Beta2_adaptin/TBP_C_dom.
IPR000814. TBP.
IPR030491. TBP_CS.
[Graphical view]
PfamiPF00352. TBP. 2 hits.
[Graphical view]
PRINTSiPR00686. TIFACTORIID.
PROSITEiPS00351. TFIID. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Arabidopsis thaliana contains two genes for TFIID."
    Gasch A., Hoffmann A., Horikoshi M., Roeder R.G., Chua N.-H.
    Nature 346:390-394(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Binary protein-protein interactions of the Arabidopsis thaliana general transcription factor IId."
    Lawit S.J., Gurley W.B.
    Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
    Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
    DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  7. "ORF cloning and analysis of Arabidopsis transcription factor genes."
    Fujita M., Mizukado S., Seki M., Shinozaki K., Mitsuda N., Takiguchi Y., Takagi M.
    Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  8. Cited for: INTERACTION WITH TAF1.
  9. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT THR-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Arabidopsis CBP1 is a novel regulator of transcription initiation in central cell-mediated pollen tube guidance."
    Li H.J., Zhu S.S., Zhang M.X., Wang T., Liang L., Xue Y., Shi D.Q., Liu J., Yang W.C.
    Plant Cell 27:2880-2893(2015) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MEE12/CCG1.
  11. "2.1 A resolution refined structure of a TATA box-binding protein (TBP)."
    Nikolov D.B., Burley S.K.
    Nat. Struct. Biol. 1:621-637(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 7-199.
  12. "Crystal structure of a TFIIB-TBP-TATA-element ternary complex."
    Nikolov D.B., Chen H., Halay E.D., Usheva A.A., Hisatake K., Lee D.K., Roeder R.G., Burley S.K.
    Nature 377:119-128(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF COMPLEX WITH TFIIB.
  13. "TATA element recognition by the TATA box-binding protein has been conserved throughout evolution."
    Patikoglou G.A., Kim J.L., Sun L., Yang S.H., Kodadek T., Burley S.K.
    Genes Dev. 13:3217-3230(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).

Entry informationi

Entry nameiTBP1_ARATH
AccessioniPrimary (citable) accession number: P28147
Secondary accession number(s): Q53YT8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: June 8, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.