P28147 (TBP1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
TATA-box-binding protein 1

Alternative name(s):
TATA sequence-binding protein 1
Short name=TBP-1
TATA-binding factor 1
TATA-box factor 1
Transcription initiation factor TFIID TBP-1 subunit

Gene names

Name:	TBP1
Ordered Locus Names:	At3g13445
ORF Names:	MRP15.10

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	200 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II.
Subunit structure	Belongs to the TFIID complex together with the TBP-associated factors (TAFs). Binds DNA as monomer.
Subcellular location	Nucleus.
Sequence similarities	Belongs to the TBP family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Repeat
Ligand	DNA-binding
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro transcription initiation from RNA polymerase II promoter Inferred from electronic annotation. Source: InterPro
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
HSFA1A	P41151	3	EBI-1247453,EBI-1544927

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: P28147-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 200

200

TATA-box-binding protein 1

PRO_0000153976

Regions

Repeat

25 – 101

77

1

Repeat

115 – 192

78

2

Secondary structure

1

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200

Helix Strand Turn

Details...

Beta strand

24 – 33

10

Helix

40 – 46

7

Turn

54 – 56

3

Beta strand

58 – 64

7

Turn

65 – 68

4

Beta strand

69 – 73

5

Beta strand

77 – 86

10

Helix

87 – 103

17

Beta strand

111 – 123

13

Helix

130 – 136

7

Turn

137 – 140

4

Turn

145 – 147

3

Beta strand

149 – 155

7

Turn

156 – 159

4

Beta strand

160 – 164

5

Beta strand

168 – 177

10

Helix

178 – 194

17

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 9E15DC2308818919	FASTA	200	22,368
	10 20 30 40 50 60 MTDQGLEGSN PVDLSKHPSG IVPTLQNIVS TVNLDCKLDL KAIALQARNA EYNPKRFAAV 70 80 90 100 110 120 IMRIREPKTT ALIFASGKMV CTGAKSEDFS KMAARKYARI VQKLGFPAKF KDFKIQNIVG 130 140 150 160 170 180 SCDVKFPIRL EGLAYSHAAF SSYEPELFPG LIYRMKVPKI VLLIFVSGKI VITGAKMRDE 190 200 TYKAFENIYP VLSEFRKIQQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Arabidopsis thaliana contains two genes for TFIID." Gasch A., Hoffmann A., Horikoshi M., Roeder R.G., Chua N.-H. Nature 346:390-394(1990) [PubMed: 2197561] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S. DNA Res. 7:217-221(2000) [PubMed: 10907853] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"2.1 A resolution refined structure of a TATA box-binding protein (TBP)." Nikolov D.B., Burley S.K. Nat. Struct. Biol. 1:621-637(1994) [PubMed: 7634102] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 7-199.
[7]	"Crystal structure of a TFIIB-TBP-TATA-element ternary complex." Nikolov D.B., Chen H., Halay E.D., Usheva A.A., Hisatake K., Lee D.K., Roeder R.G., Burley S.K. Nature 377:119-128(1995) [PubMed: 7675079] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF COMPLEX WITH TFIIB.
[8]	"TATA element recognition by the TATA box-binding protein has been conserved throughout evolution." Patikoglou G.A., Kim J.L., Sun L., Yang S.H., Kodadek T., Burley S.K. Genes Dev. 13:3217-3230(1999) [PubMed: 10617571] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X54996 mRNA. Translation: CAA38743.1.
AP000603 Genomic DNA. Translation: BAB01751.1.
CP002686 Genomic DNA. Translation: AEE75355.1.
AF324696 mRNA. Translation: AAG40047.1.
AF327429 mRNA. Translation: AAG42019.1.
AF349523 mRNA. Translation: AAK15570.1.
AY054608 mRNA. Translation: AAK96799.1.
AY072455 mRNA. Translation: AAL66870.1.
AY084881 mRNA. Translation: AAM61444.1.

IPI

IPI00529932.

PIR

S10946.

RefSeq

NP_187953.1. NM_112190.5.

UniGene

At.20074.
At.24512.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1QN3	X-ray	1.95	A/B	1-200	[»]
1QN4	X-ray	1.86	A/B	1-200	[»]
1QN5	X-ray	1.93	A/B	1-200	[»]
1QN6	X-ray	2.10	A/B	1-200	[»]
1QN7	X-ray	2.30	A/B	1-200	[»]
1QN8	X-ray	2.10	A/B	1-200	[»]
1QN9	X-ray	1.90	A/B	1-200	[»]
1QNA	X-ray	1.80	A/B	1-200	[»]
1QNB	X-ray	2.23	A/B	1-200	[»]
1QNC	X-ray	2.30	A/B	1-200	[»]
1QNE	X-ray	1.90	A/B	1-200	[»]
1VOK	X-ray	2.10	A/B	1-200	[»]
1VOL	X-ray	2.70	B	1-200	[»]
1VTL	X-ray	2.25	E/F	13-198	[»]
1VTO	X-ray	1.90	A/B	11-200	[»]

ProteinModelPortal

P28147.

SMR

P28147. Positions 12-197.

ModBase

Search...

Protein-protein interaction databases

IntAct

P28147. 3 interactions.

STRING

P28147.

Proteomic databases

PRIDE

P28147.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblPlants

AT3G13445.1; AT3G13445.1; AT3G13445.

GeneID

820546.

GenomeReviews

Gene locus AT3G13445 in contig BA000014_GR.

KEGG

ath:AT3G13445.

Organism-specific databases

TAIR

At3g13445.

Phylogenomic databases

eggNOG

KOG3302.

HOGENOM

HBG499930.

InParanoid

P28147.

OMA

STQIGEN.

PhylomeDB

P28147.

ProtClustDB

PLN00062.

Gene expression databases

ArrayExpress

P28147.

Genevestigator

P28147.

Family and domain databases

InterPro

IPR000814. TBP.
IPR012294. TFIID_C/glycos_N.
[Graphical view]

KO

K03120.

PANTHER

PTHR10126. TBP. 1 hit.

Pfam

PF00352. TBP. 2 hits.
[Graphical view]

PRINTS

PR00686. TIFACTORIID.

SUPFAM

SSF55945. TFIID_C/glycos_N. 2 hits.

PROSITE

PS00351. TFIID. 2 hits.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

TBP1_ARATH

Accession

Primary (citable) accession number: P28147

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	January 25, 2012
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program