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P28077

- PSB9_RAT

UniProt

P28077 - PSB9_RAT

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Protein

Proteasome subunit beta type-9

Gene

Psmb9

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides.

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei20 – 212Cleavage; by autocatalysisBy similarity
Active sitei21 – 211NucleophileBy similarity

GO - Molecular functioni

  1. threonine-type endopeptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. immune system process Source: UniProtKB-KW
  2. proteolysis involved in cellular protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Keywords - Biological processi

Immunity

Protein family/group databases

MEROPSiT01.013.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit beta type-9 (EC:3.4.25.1)
Alternative name(s):
Low molecular mass protein 2
Macropain chain 7
Multicatalytic endopeptidase complex chain 7
Proteasome chain 7
Proteasome subunit beta-1i
Really interesting new gene 12 protein
Gene namesi
Name:Psmb9
Synonyms:Lmp2, Ring12
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi3427. Psmb9.

Subcellular locationi

Cytoplasm PROSITE-ProRule annotation. Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. nucleus Source: UniProtKB-KW
  3. proteasome core complex Source: InterPro
  4. spermatoproteasome complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 2020Removed in mature form1 PublicationPRO_0000026631Add
BLAST
Chaini21 – 219199Proteasome subunit beta type-9PRO_0000026632Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei53 – 531N6-acetyllysineBy similarity
Modified residuei109 – 1091N6-acetyllysineBy similarity

Post-translational modificationi

Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity By similarity.By similarity

Keywords - PTMi

Acetylation, Zymogen

Proteomic databases

PaxDbiP28077.
PRIDEiP28077.

Expressioni

Tissue specificityi

Detected in the cytoplasmic lobe of elongated spermatids, in residual bodies, and in the acrosomal cap of round spermatids.1 Publication

Inductioni

Up-regulated by interferon gamma (at protein level). Up-regulated by theophylline (THP), a reprotoxic agent thought to induce infertility.1 Publication

Gene expression databases

GenevestigatoriP28077.

Interactioni

Subunit structurei

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Component of the immunoproteasome, where it displaces the equivalent houskeeping subunit PSMB6. Component of the spermatoproteasome, a form of the proteasome specifically found in testis. Interacts with NCOA2 and NCOA3 By similarity.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000000532.

Structurei

3D structure databases

ProteinModelPortaliP28077.
SMRiP28077. Positions 21-216.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG291022.
HOGENOMiHOG000091079.
HOVERGENiHBG000123.
InParanoidiP28077.
KOiK02741.
PhylomeDBiP28077.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
PRINTSiPR00141. PROTEASOME.
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28077-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLQAGAPTAG SFRTGEVHTG TTIMAVEFDG GVVVGSDSRV SAGAAVVNRV
60 70 80 90 100
FDKLSPLHQR IYCALSGSAA DAQAIADMAA YQLELHGLEL EEPPLVLAAA
110 120 130 140 150
NIVKNISYKY REDLLAHLMV AGWDQHEGGQ VYGTMGGMLI RQPFAIGGSG
160 170 180 190 200
STYIYGYVDA AYKPGMTPEE CRRFTTDAIT LAMNRDGSSG GVIYLVTITA
210
DGVDHRVILG DELPKFYDE
Length:219
Mass (Da):23,324
Last modified:February 1, 1994 - v2
Checksum:iE713C94AF33836E4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D10757 mRNA. Translation: BAA01589.1.
PIRiJX0231.
S09088.
RefSeqiNP_036840.2. NM_012708.2.
UniGeneiRn.13686.

Genome annotation databases

GeneIDi24967.
KEGGirno:24967.
UCSCiRGD:3427. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D10757 mRNA. Translation: BAA01589.1 .
PIRi JX0231.
S09088.
RefSeqi NP_036840.2. NM_012708.2.
UniGenei Rn.13686.

3D structure databases

ProteinModelPortali P28077.
SMRi P28077. Positions 21-216.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000000532.

Protein family/group databases

MEROPSi T01.013.

Proteomic databases

PaxDbi P28077.
PRIDEi P28077.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 24967.
KEGGi rno:24967.
UCSCi RGD:3427. rat.

Organism-specific databases

CTDi 5698.
RGDi 3427. Psmb9.

Phylogenomic databases

eggNOGi NOG291022.
HOGENOMi HOG000091079.
HOVERGENi HBG000123.
InParanoidi P28077.
KOi K02741.
PhylomeDBi P28077.

Miscellaneous databases

NextBioi 605004.
PROi P28077.

Gene expression databases

Genevestigatori P28077.

Family and domain databases

Gene3Di 3.60.20.10. 1 hit.
InterProi IPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view ]
Pfami PF00227. Proteasome. 1 hit.
[Graphical view ]
PRINTSi PR00141. PROTEASOME.
SUPFAMi SSF56235. SSF56235. 1 hit.
PROSITEi PS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning of cDNAs for rat proteasomes: deduced primary structures of four other subunits."
    Tamura T., Shimbara N., Aki M., Ishida N., Bey F., Scherrer K., Tanaka K., Ichihara A.
    J. Biochem. 112:530-534(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "N-terminal sequence similarities between components of the multicatalytic proteinase complex."
    Lilley K.S., Davison M.D., Rivett A.J.
    FEBS Lett. 262:327-329(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 21-41.
  3. "Differential expression of genes encoding constitutive and inducible 20S proteasomal core subunits in the testis and epididymis of theophylline- or 1,3-dinitrobenzene-exposed rats."
    Tengowski M.W., Feng D., Sutovsky M., Sutovsky P.
    Biol. Reprod. 76:149-163(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY THP, TISSUE SPECIFICITY.

Entry informationi

Entry nameiPSB9_RAT
AccessioniPrimary (citable) accession number: P28077
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: February 1, 1994
Last modified: October 29, 2014
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3