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P28077

- PSB9_RAT

UniProt

P28077 - PSB9_RAT

Protein

Proteasome subunit beta type-9

Gene

Psmb9

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 2 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides.

    Catalytic activityi

    Cleavage of peptide bonds with very broad specificity.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei20 – 212Cleavage; by autocatalysisBy similarity
    Active sitei21 – 211NucleophileBy similarity

    GO - Molecular functioni

    1. threonine-type endopeptidase activity Source: UniProtKB-KW

    GO - Biological processi

    1. immune system process Source: UniProtKB-KW
    2. proteolysis involved in cellular protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Threonine protease

    Keywords - Biological processi

    Immunity

    Protein family/group databases

    MEROPSiT01.013.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proteasome subunit beta type-9 (EC:3.4.25.1)
    Alternative name(s):
    Low molecular mass protein 2
    Macropain chain 7
    Multicatalytic endopeptidase complex chain 7
    Proteasome chain 7
    Proteasome subunit beta-1i
    Really interesting new gene 12 protein
    Gene namesi
    Name:Psmb9
    Synonyms:Lmp2, Ring12
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi3427. Psmb9.

    Subcellular locationi

    Cytoplasm PROSITE-ProRule annotation. Nucleus By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell
    3. proteasome core complex Source: InterPro
    4. spermatoproteasome complex Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus, Proteasome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei1 – 2020Removed in mature form1 PublicationPRO_0000026631Add
    BLAST
    Chaini21 – 219199Proteasome subunit beta type-9PRO_0000026632Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei53 – 531N6-acetyllysineBy similarity
    Modified residuei109 – 1091N6-acetyllysineBy similarity

    Post-translational modificationi

    Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity By similarity.By similarity

    Keywords - PTMi

    Acetylation, Zymogen

    Proteomic databases

    PaxDbiP28077.
    PRIDEiP28077.

    Expressioni

    Tissue specificityi

    Detected in the cytoplasmic lobe of elongated spermatids, in residual bodies, and in the acrosomal cap of round spermatids.1 Publication

    Inductioni

    Up-regulated by interferon gamma (at protein level). Up-regulated by theophylline (THP), a reprotoxic agent thought to induce infertility.1 Publication

    Gene expression databases

    GenevestigatoriP28077.

    Interactioni

    Subunit structurei

    The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Component of the immunoproteasome, where it displaces the equivalent houskeeping subunit PSMB6. Component of the spermatoproteasome, a form of the proteasome specifically found in testis. Interacts with NCOA2 and NCOA3 By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000000532.

    Structurei

    3D structure databases

    ProteinModelPortaliP28077.
    SMRiP28077. Positions 21-216.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase T1B family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG291022.
    HOGENOMiHOG000091079.
    HOVERGENiHBG000123.
    InParanoidiP28077.
    KOiK02741.
    PhylomeDBiP28077.

    Family and domain databases

    Gene3Di3.60.20.10. 1 hit.
    InterProiIPR029055. Ntn_hydrolases_N.
    IPR000243. Pept_T1A_subB.
    IPR016050. Proteasome_bsu_CS.
    IPR001353. Proteasome_sua/b.
    IPR023333. Proteasome_suB-type.
    [Graphical view]
    PfamiPF00227. Proteasome. 1 hit.
    [Graphical view]
    PRINTSiPR00141. PROTEASOME.
    SUPFAMiSSF56235. SSF56235. 1 hit.
    PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
    PS51476. PROTEASOME_BETA_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P28077-1 [UniParc]FASTAAdd to Basket

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    MLQAGAPTAG SFRTGEVHTG TTIMAVEFDG GVVVGSDSRV SAGAAVVNRV    50
    FDKLSPLHQR IYCALSGSAA DAQAIADMAA YQLELHGLEL EEPPLVLAAA 100
    NIVKNISYKY REDLLAHLMV AGWDQHEGGQ VYGTMGGMLI RQPFAIGGSG 150
    STYIYGYVDA AYKPGMTPEE CRRFTTDAIT LAMNRDGSSG GVIYLVTITA 200
    DGVDHRVILG DELPKFYDE 219
    Length:219
    Mass (Da):23,324
    Last modified:February 1, 1994 - v2
    Checksum:iE713C94AF33836E4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D10757 mRNA. Translation: BAA01589.1.
    PIRiJX0231.
    S09088.
    RefSeqiNP_036840.2. NM_012708.2.
    UniGeneiRn.13686.

    Genome annotation databases

    GeneIDi24967.
    KEGGirno:24967.
    UCSCiRGD:3427. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D10757 mRNA. Translation: BAA01589.1 .
    PIRi JX0231.
    S09088.
    RefSeqi NP_036840.2. NM_012708.2.
    UniGenei Rn.13686.

    3D structure databases

    ProteinModelPortali P28077.
    SMRi P28077. Positions 21-216.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000000532.

    Protein family/group databases

    MEROPSi T01.013.

    Proteomic databases

    PaxDbi P28077.
    PRIDEi P28077.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 24967.
    KEGGi rno:24967.
    UCSCi RGD:3427. rat.

    Organism-specific databases

    CTDi 5698.
    RGDi 3427. Psmb9.

    Phylogenomic databases

    eggNOGi NOG291022.
    HOGENOMi HOG000091079.
    HOVERGENi HBG000123.
    InParanoidi P28077.
    KOi K02741.
    PhylomeDBi P28077.

    Miscellaneous databases

    NextBioi 605004.
    PROi P28077.

    Gene expression databases

    Genevestigatori P28077.

    Family and domain databases

    Gene3Di 3.60.20.10. 1 hit.
    InterProi IPR029055. Ntn_hydrolases_N.
    IPR000243. Pept_T1A_subB.
    IPR016050. Proteasome_bsu_CS.
    IPR001353. Proteasome_sua/b.
    IPR023333. Proteasome_suB-type.
    [Graphical view ]
    Pfami PF00227. Proteasome. 1 hit.
    [Graphical view ]
    PRINTSi PR00141. PROTEASOME.
    SUPFAMi SSF56235. SSF56235. 1 hit.
    PROSITEi PS00854. PROTEASOME_BETA_1. 1 hit.
    PS51476. PROTEASOME_BETA_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of cDNAs for rat proteasomes: deduced primary structures of four other subunits."
      Tamura T., Shimbara N., Aki M., Ishida N., Bey F., Scherrer K., Tanaka K., Ichihara A.
      J. Biochem. 112:530-534(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "N-terminal sequence similarities between components of the multicatalytic proteinase complex."
      Lilley K.S., Davison M.D., Rivett A.J.
      FEBS Lett. 262:327-329(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 21-41.
    3. "Differential expression of genes encoding constitutive and inducible 20S proteasomal core subunits in the testis and epididymis of theophylline- or 1,3-dinitrobenzene-exposed rats."
      Tengowski M.W., Feng D., Sutovsky M., Sutovsky P.
      Biol. Reprod. 76:149-163(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION BY THP, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiPSB9_RAT
    AccessioniPrimary (citable) accession number: P28077
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 114 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3