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P28077 (PSB9_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Proteasome subunit beta type-9

EC=3.4.25.1
Alternative name(s):
Low molecular mass protein 2
Macropain chain 7
Multicatalytic endopeptidase complex chain 7
Proteasome chain 7
Proteasome subunit beta-1i
Really interesting new gene 12 protein
Gene names
Name:Psmb9
Synonyms:Lmp2, Ring12
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length219 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides.

Catalytic activity

Cleavage of peptide bonds with very broad specificity.

Subunit structure

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Component of the immunoproteasome, where it displaces the equivalent houskeeping subunit PSMB6. Component of the spermatoproteasome, a form of the proteasome specifically found in testis. Interacts with NCOA2 and NCOA3 By similarity.

Subcellular location

Cytoplasm By similarity. Nucleus By similarity.

Tissue specificity

Detected in the cytoplasmic lobe of elongated spermatids, in residual bodies, and in the acrosomal cap of round spermatids. Ref.3

Induction

Up-regulated by interferon gamma (at protein level). Up-regulated by theophylline (THP), a reprotoxic agent thought to induce infertility. Ref.3

Post-translational modification

Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity By similarity.

Sequence similarities

Belongs to the peptidase T1B family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Propeptide1 – 2020Removed in mature form
PRO_0000026631
Chain21 – 219199Proteasome subunit beta type-9
PRO_0000026632

Sites

Active site211Nucleophile By similarity
Site20 – 212Cleavage; by autocatalysis By similarity

Amino acid modifications

Modified residue531N6-acetyllysine By similarity
Modified residue1091N6-acetyllysine By similarity

Sequences

Sequence LengthMass (Da)Tools
P28077 [UniParc].

Last modified February 1, 1994. Version 2.
Checksum: E713C94AF33836E4

FASTA21923,324
        10         20         30         40         50         60 
MLQAGAPTAG SFRTGEVHTG TTIMAVEFDG GVVVGSDSRV SAGAAVVNRV FDKLSPLHQR 

        70         80         90        100        110        120 
IYCALSGSAA DAQAIADMAA YQLELHGLEL EEPPLVLAAA NIVKNISYKY REDLLAHLMV 

       130        140        150        160        170        180 
AGWDQHEGGQ VYGTMGGMLI RQPFAIGGSG STYIYGYVDA AYKPGMTPEE CRRFTTDAIT 

       190        200        210 
LAMNRDGSSG GVIYLVTITA DGVDHRVILG DELPKFYDE 

« Hide

References

[1]"Molecular cloning of cDNAs for rat proteasomes: deduced primary structures of four other subunits."
Tamura T., Shimbara N., Aki M., Ishida N., Bey F., Scherrer K., Tanaka K., Ichihara A.
J. Biochem. 112:530-534(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"N-terminal sequence similarities between components of the multicatalytic proteinase complex."
Lilley K.S., Davison M.D., Rivett A.J.
FEBS Lett. 262:327-329(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 21-41.
[3]"Differential expression of genes encoding constitutive and inducible 20S proteasomal core subunits in the testis and epididymis of theophylline- or 1,3-dinitrobenzene-exposed rats."
Tengowski M.W., Feng D., Sutovsky M., Sutovsky P.
Biol. Reprod. 76:149-163(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION BY THP, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D10757 mRNA. Translation: BAA01589.1.
PIRJX0231.
S09088.
RefSeqNP_036840.2. NM_012708.2.
UniGeneRn.13686.

3D structure databases

ProteinModelPortalP28077.
SMRP28077. Positions 21-216.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000000532.

Protein family/group databases

MEROPST01.013.

Proteomic databases

PaxDbP28077.
PRIDEP28077.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID24967.
KEGGrno:24967.
UCSCRGD:3427. rat.

Organism-specific databases

CTD5698.
RGD3427. Psmb9.

Phylogenomic databases

eggNOGNOG291022.
HOGENOMHOG000091079.
HOVERGENHBG000123.
InParanoidP28077.
KOK02741.
PhylomeDBP28077.

Gene expression databases

GenevestigatorP28077.

Family and domain databases

Gene3D3.60.20.10. 1 hit.
InterProIPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamPF00227. Proteasome. 1 hit.
[Graphical view]
PRINTSPR00141. PROTEASOME.
SUPFAMSSF56235. SSF56235. 1 hit.
PROSITEPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio605004.
PROP28077.

Entry information

Entry namePSB9_RAT
AccessionPrimary (citable) accession number: P28077
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: February 1, 1994
Last modified: June 11, 2014
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries