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Protein

Proteasome subunit beta type-9

Gene

Psmb9

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides.

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei20 – 212Cleavage; by autolysisBy similarity
Active sitei21 – 211NucleophileBy similarity

GO - Molecular functioni

  • endopeptidase activity Source: GO_Central
  • proteasome binding Source: RGD
  • threonine-type endopeptidase activity Source: UniProtKB-KW

GO - Biological processi

  • cellular response to electrical stimulus Source: RGD
  • cellular response to interleukin-1 Source: RGD
  • cellular response to virus Source: RGD
  • liver development Source: RGD
  • muscle atrophy Source: RGD
  • proteasomal ubiquitin-independent protein catabolic process Source: GO_Central
  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: GO_Central
  • response to alkaloid Source: RGD
  • response to benzene Source: RGD
  • response to drug Source: RGD
  • spleen development Source: RGD
  • thymus development Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Keywords - Biological processi

Immunity

Protein family/group databases

MEROPSiT01.013.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit beta type-9 (EC:3.4.25.1)
Alternative name(s):
Low molecular mass protein 2
Macropain chain 7
Multicatalytic endopeptidase complex chain 7
Proteasome chain 7
Proteasome subunit beta-1i
Really interesting new gene 12 protein
Gene namesi
Name:Psmb9
Synonyms:Lmp2, Ring12
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3427. Psmb9.

Subcellular locationi

  • Cytoplasm PROSITE-ProRule annotation
  • Nucleus By similarity

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • cytosol Source: RGD
  • nucleus Source: UniProtKB-SubCell
  • proteasome complex Source: RGD
  • proteasome core complex Source: GO_Central
  • spermatoproteasome complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 2020Removed in mature form1 PublicationPRO_0000026631Add
BLAST
Chaini21 – 219199Proteasome subunit beta type-9PRO_0000026632Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei53 – 531N6-acetyllysineBy similarity
Modified residuei109 – 1091N6-acetyllysineBy similarity

Post-translational modificationi

Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity.By similarity

Keywords - PTMi

Acetylation, Zymogen

Proteomic databases

PaxDbiP28077.
PRIDEiP28077.

Expressioni

Tissue specificityi

Detected in the cytoplasmic lobe of elongated spermatids, in residual bodies, and in the acrosomal cap of round spermatids.1 Publication

Inductioni

Up-regulated by interferon gamma (at protein level). Up-regulated by theophylline (THP), a reprotoxic agent thought to induce infertility.1 Publication

Gene expression databases

GenevisibleiP28077. RN.

Interactioni

Subunit structurei

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Component of the immunoproteasome, where it displaces the equivalent houskeeping subunit PSMB6. Component of the spermatoproteasome, a form of the proteasome specifically found in testis. Interacts with NCOA2 and NCOA3 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000000532.

Structurei

3D structure databases

ProteinModelPortaliP28077.
SMRiP28077. Positions 21-216.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG291022.
HOGENOMiHOG000091079.
HOVERGENiHBG000123.
InParanoidiP28077.
KOiK02741.
PhylomeDBiP28077.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
PRINTSiPR00141. PROTEASOME.
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28077-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLQAGAPTAG SFRTGEVHTG TTIMAVEFDG GVVVGSDSRV SAGAAVVNRV
60 70 80 90 100
FDKLSPLHQR IYCALSGSAA DAQAIADMAA YQLELHGLEL EEPPLVLAAA
110 120 130 140 150
NIVKNISYKY REDLLAHLMV AGWDQHEGGQ VYGTMGGMLI RQPFAIGGSG
160 170 180 190 200
STYIYGYVDA AYKPGMTPEE CRRFTTDAIT LAMNRDGSSG GVIYLVTITA
210
DGVDHRVILG DELPKFYDE
Length:219
Mass (Da):23,324
Last modified:February 1, 1994 - v2
Checksum:iE713C94AF33836E4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10757 mRNA. Translation: BAA01589.1.
PIRiJX0231.
S09088.
RefSeqiNP_036840.2. NM_012708.2.
UniGeneiRn.13686.

Genome annotation databases

GeneIDi24967.
KEGGirno:24967.
UCSCiRGD:3427. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10757 mRNA. Translation: BAA01589.1.
PIRiJX0231.
S09088.
RefSeqiNP_036840.2. NM_012708.2.
UniGeneiRn.13686.

3D structure databases

ProteinModelPortaliP28077.
SMRiP28077. Positions 21-216.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000000532.

Protein family/group databases

MEROPSiT01.013.

Proteomic databases

PaxDbiP28077.
PRIDEiP28077.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi24967.
KEGGirno:24967.
UCSCiRGD:3427. rat.

Organism-specific databases

CTDi5698.
RGDi3427. Psmb9.

Phylogenomic databases

eggNOGiNOG291022.
HOGENOMiHOG000091079.
HOVERGENiHBG000123.
InParanoidiP28077.
KOiK02741.
PhylomeDBiP28077.

Miscellaneous databases

NextBioi605004.
PROiP28077.

Gene expression databases

GenevisibleiP28077. RN.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
PRINTSiPR00141. PROTEASOME.
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning of cDNAs for rat proteasomes: deduced primary structures of four other subunits."
    Tamura T., Shimbara N., Aki M., Ishida N., Bey F., Scherrer K., Tanaka K., Ichihara A.
    J. Biochem. 112:530-534(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "N-terminal sequence similarities between components of the multicatalytic proteinase complex."
    Lilley K.S., Davison M.D., Rivett A.J.
    FEBS Lett. 262:327-329(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 21-41.
  3. "Differential expression of genes encoding constitutive and inducible 20S proteasomal core subunits in the testis and epididymis of theophylline- or 1,3-dinitrobenzene-exposed rats."
    Tengowski M.W., Feng D., Sutovsky M., Sutovsky P.
    Biol. Reprod. 76:149-163(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY THP, TISSUE SPECIFICITY.

Entry informationi

Entry nameiPSB9_RAT
AccessioniPrimary (citable) accession number: P28077
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: February 1, 1994
Last modified: June 24, 2015
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.