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Protein

Proteasome subunit beta type-5

Gene

Psmb5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). Within the 20S core complex, PSMB5 displays a chymotrypsin-like activity.By similarity

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei60NucleophileBy similarity1
Binding sitei108Bortezomib; via amide nitrogenBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Threonine protease

Protein family/group databases

MEROPSiT01.012.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit beta type-5 (EC:3.4.25.1By similarity)
Alternative name(s):
Macropain epsilon chain
Multicatalytic endopeptidase complex epsilon chain
Proteasome chain 6
Proteasome epsilon chain
Proteasome subunit X
Gene namesi
Name:Psmb5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi61879. Psmb5.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000265931 – 59Removed in mature form1 PublicationAdd BLAST59
ChainiPRO_000002659460 – 263Proteasome subunit beta type-5Add BLAST204

Keywords - PTMi

Zymogen

Proteomic databases

PaxDbiP28075.
PRIDEiP28075.

PTM databases

SwissPalmiP28075.

Expressioni

Inductioni

Down-regulated by theophylline (THP) and up-regulated by 1,3-dinitrobenzene (DNB), two reprotoxic agents thought to induce infertility.1 Publication

Interactioni

Subunit structurei

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is a barrel-shaped complex made of 28 subunits that are arranged in four stacked rings. The two outer rings are each formed by seven alpha subunits, and the two inner rings are formed by seven beta subunits. The proteolytic activity is exerted by three beta-subunits PSMB5, PSMB6 and PSMB7. Directly interacts with POMP. Interacts with ABCB1 and TAP1.By similarity

Protein-protein interaction databases

BioGridi248073. 2 interactors.
IntActiP28075. 1 interactor.
STRINGi10116.ENSRNOP00000018005.

Structurei

3D structure databases

ProteinModelPortaliP28075.
SMRiP28075.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0175. Eukaryota.
ENOG410XQRP. LUCA.
HOGENOMiHOG000091082.
HOVERGENiHBG108297.
InParanoidiP28075.
KOiK02737.
PhylomeDBiP28075.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiView protein in InterPro
IPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
PfamiView protein in Pfam
PF00227. Proteasome. 1 hit.
PRINTSiPR00141. PROTEASOME.
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiView protein in PROSITE
PS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28075-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALASVLQRP MPVNQHGFFG LGGRADLLDL GPGSPGDGLS LAAPSWGVPE
60 70 80 90 100
EPRIEMLHGT TTLAFKFQHG VIVAADSRAT AGPYIASQTV KKVIEINPYL
110 120 130 140 150
LGTMAGGAAD CSFWERLLAR QCRIYELRNK ERISVAAASK LLANMVYQYK
160 170 180 190 200
GMGLSMGTMI CGWDKRGPGL YYVDSEGNRI SGTAFSVGSG SVYAFGVMDR
210 220 230 240 250
GYSYDLQVEE AYDLARRAIY QATYRDAYSG GAVNLYHVRE DGWIRVSSDN
260
VADLHDKYTS SIP
Length:263
Mass (Da):28,585
Last modified:April 29, 2008 - v3
Checksum:i317FC0C827FE65CA
GO

Sequence cautioni

The sequence BAA08204 differs from that shown. Sequencing error.Curated
The sequence BAA08204 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti62T → I AA sequence (PubMed:2335214).Curated1
Sequence conflicti69H → E AA sequence (PubMed:2335214).Curated1
Sequence conflicti73V → L AA sequence (PubMed:2335214).Curated1
Sequence conflicti83P → A in EDM14180 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D45247 mRNA. Translation: BAA08204.1. Sequence problems.
CH474049 Genomic DNA. Translation: EDM14180.1.
PIRiS09087.
RefSeqiNP_001099197.2. NM_001105727.2.
UniGeneiRn.2.

Genome annotation databases

GeneIDi29425.
KEGGirno:29425.
UCSCiRGD:61879. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D45247 mRNA. Translation: BAA08204.1. Sequence problems.
CH474049 Genomic DNA. Translation: EDM14180.1.
PIRiS09087.
RefSeqiNP_001099197.2. NM_001105727.2.
UniGeneiRn.2.

3D structure databases

ProteinModelPortaliP28075.
SMRiP28075.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248073. 2 interactors.
IntActiP28075. 1 interactor.
STRINGi10116.ENSRNOP00000018005.

Protein family/group databases

MEROPSiT01.012.

PTM databases

SwissPalmiP28075.

Proteomic databases

PaxDbiP28075.
PRIDEiP28075.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi29425.
KEGGirno:29425.
UCSCiRGD:61879. rat.

Organism-specific databases

CTDi5693.
RGDi61879. Psmb5.

Phylogenomic databases

eggNOGiKOG0175. Eukaryota.
ENOG410XQRP. LUCA.
HOGENOMiHOG000091082.
HOVERGENiHBG108297.
InParanoidiP28075.
KOiK02737.
PhylomeDBiP28075.

Miscellaneous databases

PROiPR:P28075.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiView protein in InterPro
IPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
PfamiView protein in Pfam
PF00227. Proteasome. 1 hit.
PRINTSiPR00141. PROTEASOME.
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiView protein in PROSITE
PS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPSB5_RAT
AccessioniPrimary (citable) accession number: P28075
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: April 29, 2008
Last modified: May 10, 2017
This is version 130 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.