Reviewed,
UniProtKB/Swiss-Prot P28074 (PSB5_HUMAN)
Last modified
July 7, 2009.
Version 107.
History...
Clusters with 100%,
90%,
50% identity |
Documents (6) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Proteasome subunit beta type-5 EC=3.4.25.1 Alternative name(s): Proteasome epsilon chain Macropain epsilon chain Multicatalytic endopeptidase complex epsilon chain Proteasome subunit X Proteasome chain 6 Proteasome subunit MB1 | ||||
| Gene names |
| ||||
| Organism | Homo sapiens (Human) [Complete proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 263 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. May catalyze basal processing of intracellular antigens. |
| Catalytic activity | Cleavage of peptide bonds with very broad specificity. |
| Subunit structure | The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. This subunit can be displaced by the equivalent immune-specific subunit PSMB8. Directly interacts with POMP. Ref.11 |
| Subcellular location | |
| Sequence similarities | Belongs to the peptidase T1B family. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| LSM1 | O15116 | 1 | EBI-357828,EBI-347619 | |
| PSMD6 | Q15008 | 1 | EBI-357828,EBI-359701 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Propeptide | 1 – 59 | 59 | Ref.7 | PRO_0000026589 | |||||
| Chain | 60 – 263 | 204 | Proteasome subunit beta type-5 | PRO_0000026590 | |||||
Sites | |||||||||
| Active site | 60 | 1 | Nucleophile | ||||||
Natural variations | |||||||||
| Natural variant | 24 | 1 | R → C: dbSNP rs11543947. | VAR_051549 | |||||
Experimental info | |||||||||
| Sequence conflict | 3 – 6 | 4 | LASV → IRGR in BAA06097. Ref.4 | ||||||
| Sequence conflict | 3 – 5 | 3 | LAS → HEG in BC004146. Ref.3 | ||||||
| Sequence conflict | 85 | 1 | I → F AA sequence Ref.7 | ||||||
| Sequence conflict | 109 | 1 | A → G in AAB33092. Ref.5 | ||||||
| Sequence conflict | 158 | 1 | T → S in AAB33092. Ref.5 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Divergent intron arrangement in the MB1/LMP7 proteasome gene pair." Abdulla S., Beck S., Belich M., Jackson A., Nakamura T., Trowsdale J. Immunogenetics 44:254-258(1996) [PubMed: 8753855] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Blocker H., Heubner D., Hoerlein A., Michel G., Wedler H., Kohrer K., Ottenwalder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Endometrial adenocarcinoma. |
| [3] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Hypothalamus and Skin. |
| [4] | "cDNA cloning and interferon gamma down-regulation of proteasomal subunits X and Y." Akiyama K.-Y., Yokota K.-Y., Kagawa S., Shimbara N., Tamura T., Akioka H., Nothwang H.G., Noda C., Tanaka K., Ichihara A. Science 265:1231-1234(1994) [PubMed: 8066462] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-263. |
| [5] | "Proteasome components with reciprocal expression to that of the MHC-encoded LMP proteins." Belich M.P., Glynne R.J., Senger G., Sheer D., Trowsdale J. Curr. Biol. 4:769-776(1994) [PubMed: 7820546] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 49-263. |
| [6] | "Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 56-263. |
| [7] | "Relationships among the subunits of the high molecular weight proteinase, macropain (proteasome)." Lee L.W., Moomaw C.R., Orth K., McGuire M.J., DeMartino G.N., Slaughter C.A. Biochim. Biophys. Acta 1037:178-185(1990) [PubMed: 2306472] [Abstract] Cited for: PROTEIN SEQUENCE OF 60-85. |
| [8] | "Human proteasome subunits from 2-dimensional gels identified by partial sequencing." Kristensen P., Johnsen A.H., Uerkvitz W., Tanaka K., Hendil K.B. Biochem. Biophys. Res. Commun. 205:1785-1789(1994) [PubMed: 7811265] [Abstract] Cited for: PROTEIN SEQUENCE OF 201-216 AND 226-239. |
| [9] | "Replacement of proteasome subunits X and Y by LMP7 and LMP2 induced by interferon-gamma for acquirement of the functional diversity responsible for antigen processing." Akiyama K., Kagawa S., Tamura T., Shimbara N., Takashina M., Kristensen P., Hendil K.B., Tanaka K., Ichihara A. FEBS Lett. 343:85-88(1994) [PubMed: 8163024] [Abstract] Cited for: PROTEIN SEQUENCE OF 201-216. Tissue: Kidney. |
| [10] | Erratum Kristensen P., Johnsen A.H., Uerkvitz W., Tanaka K., Hendil K.B. Biochem. Biophys. Res. Commun. 207:1059-1059(1995) [PubMed: 7864893] [Abstract] |
| [11] | "IFN-gamma-induced immune adaptation of the proteasome system is an accelerated and transient response." Heink S., Ludwig D., Kloetzel P.-M., Krueger E. Proc. Natl. Acad. Sci. U.S.A. 102:9241-9246(2005) [PubMed: 15944226] [Abstract] Cited for: INTERACTION WITH POMP. |
| [12] | "Mass spectrometric characterization of the affinity-purified human 26S proteasome complex." Wang X., Chen C.-F., Baker P.R., Chen P.-L., Kaiser P., Huang L. Biochemistry 46:3553-3565(2007) [PubMed: 17323924] [Abstract] Cited for: PROTEOLYTIC PROCESSING [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. |
| [13] | Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| X95586 Genomic DNA. Translation: CAA64838.1. BX538001 mRNA. Translation: CAD97956.1. Different initiation. BC004146 mRNA. No translation available. BC057840 mRNA. Translation: AAH57840.1. BC107720 mRNA. Translation: AAI07721.1. D29011 mRNA. Translation: BAA06097.1. Different initiation. S74378 mRNA. Translation: AAB33092.1. BT006777 mRNA. Translation: AAP35423.1. Different initiation. | |
| IPI | IPI00479306. |
| PIR | A54589. I52906. PC2328. S08189. |
| RefSeq | NP_001124197.1. NP_002788.1. |
| UniGene | Hs.422990 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1RYP based on UniProtKB P30656. |
| SMR | P28074. Positions 5-205. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP:27540N. |
| IntAct | P28074. 20 interactions. |
Protein family/group databases | |
| MEROPS | T01.012. |
PTM databases | |
| PhosphoSite | P28074. |
2-D gel databases | |
| REPRODUCTION-2DPAGE | IPI00479306. |
Proteomic databases | |
| PRIDE | P28074. |
Genome annotation databases | |
| Ensembl | ENSG00000100804. Homo sapiens. [Contig view] |
| GeneID | 5693. |
| KEGG | hsa:5693. |
| UCSC | uc001wii.1. human. |
Organism-specific databases | |
| GeneCards | GC14M022564. |
| H-InvDB | HIX0011529. HIX0026673. |
| HGNC | HGNC:9542. PSMB5. |
| MIM | 600306. gene. |
| PharmGKB | PA33887. |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOVERGEN | P28074. |
| OMA | P28074. QTFAYGV. |
Enzyme and pathway databases | |
| BRENDA | 3.4.25.1. 247. |
| Reactome | REACT_11045. Signaling by Wnt. REACT_13. Metabolism of amino acids. REACT_13635. Regulation of activated PAK-2p34 by proteasome mediated degradation. REACT_152. Cell Cycle, Mitotic. REACT_1538. Cell Cycle Checkpoints. REACT_383. DNA Replication. REACT_578. Apoptosis. REACT_6185. HIV Infection. REACT_6850. Cdc20:Phospho-APC/C mediated degradation of Cyclin A. REACT_9035. APC/C:Cdh1-mediated degradation of Skp2. |
Gene expression databases | |
| ArrayExpress | P28074. |
| Bgee | P28074. |
| CleanEx | HS_PSMB5. |
| GermOnline | ENSG00000100804. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR000243. Pept_T1A_subB. IPR001353. Proteasome_alpha_beta_su. IPR016050. Proteasome_bsu_CS. [Graphical view] |
| Pfam | PF00227. Proteasome. 1 hit. [Graphical view] |
| PRINTS | PR00141. PROTEASOME. |
| PROSITE | PS00854. PROTEASOME_B. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| DrugBank | DB00188. Bortezomib. |
| NextBio | 22114. |
| SOURCE | Search... |
Entry information
| Entry name | PSB5_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P28074 Secondary accession number(s): Q16242 Q9TNN9 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 14 Human chromosome 14: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| Peptidase families Classification of peptidase families and list of entries |
| SIMILARITY comments Index of protein domains and families |

Clusters with


