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P28064 (PSB8_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Proteasome subunit beta type-8

EC=3.4.25.1
Alternative name(s):
Macropain subunit C13
Multicatalytic endopeptidase complex subunit C13
Proteasome component C13
Proteasome subunit beta-5i
Gene names
Name:Psmb8
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length276 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Required for adipocyte differentiation By similarity.

Catalytic activity

Cleavage of peptide bonds with very broad specificity.

Subunit structure

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Component of the immunoproteasome, where it displaces the equivalent housekeeping subunit PSMB5. Component of the spermatoproteasome, a form of the proteasome specifically found in testis. Directly interacts with POMP By similarity. Interacts with TAP1 By similarity.

Subcellular location

Cytoplasm By similarity. Nucleus By similarity.

Induction

Up-regulated by interferon gamma (at protein level). Down-regulated by theophylline (THP), a reprotoxic agent thought to induce infertility. Ref.3

Post-translational modification

Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity By similarity.

Miscellaneous

Encoded in the MHC class II region.

Sequence similarities

Belongs to the peptidase T1B family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Propeptide1 – 7272Removed in mature form By similarity
PRO_0000026601
Chain73 – 276204Proteasome subunit beta type-8
PRO_0000026602

Sites

Active site731Nucleophile By similarity
Site72 – 732Cleavage; by autocatalysis By similarity

Experimental info

Sequence conflict61L → S Ref.2
Sequence conflict16 – 249EWAAVDAGS → SGLAVDAE Ref.2
Sequence conflict541L → S Ref.2

Sequences

Sequence LengthMass (Da)Tools
P28064 [UniParc].

Last modified December 20, 2005. Version 3.
Checksum: 0955C80D2F969A97

FASTA27630,570
        10         20         30         40         50         60 
MALLDLCGAP RGQRPEWAAV DAGSGLRSDP GHYSFSVQAP ELALPRGMQP TEFLRSFGDD 

        70         80         90        100        110        120 
QERKVQIEMA HGTTTLAFKF QHGVIVAVDS RASAGSYIAT IRVNKVIEIN PYLLGTMSGC 

       130        140        150        160        170        180 
AADCQYWERL LAKECRLYYL RNGERISVSA ASKLLSNMML QYRGMGLSMG SMICGWDKKG 

       190        200        210        220        230        240 
PGLYYVDDNG TRLSGQMFST GSGNTYAYGV MDSGYRQDLS PEEAYDLARR AIVYATHRDS 

       250        260        270 
YSGGVVNMYH MKKDGWVKVE STDVSDLLHK YREATL 

« Hide

References

« Hide 'large scale' references
[1]"The genomic sequence and comparative analysis of the rat major histocompatibility complex."
Hurt P., Walter L., Sudbrak R., Klages S., Mueller I., Shiina T., Inoko H., Lehrach H., Guenther E., Reinhardt R., Himmelbauer H.
Genome Res. 14:631-639(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Brown Norway.
[2]"cDNA cloning of rat proteasome subunit RC1, a homologue of RING10 located in the human MHC class II region."
Aki M., Tamura T., Fuminori T., Iwanaga S., Kawamura Y., Shimbara N., Kagawa S., Tanaka K., Ichihara A.
FEBS Lett. 301:65-68(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-276.
[3]"Differential expression of genes encoding constitutive and inducible 20S proteasomal core subunits in the testis and epididymis of theophylline- or 1,3-dinitrobenzene-exposed rats."
Tengowski M.W., Feng D., Sutovsky M., Sutovsky P.
Biol. Reprod. 76:149-163(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION BY THP AND DNB.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX883043 Genomic DNA. Translation: CAE83942.1.
D10729 mRNA. Translation: BAA01572.1.
PIRS21126.
RefSeqNP_542945.2. NM_080767.2.
UniGeneRn.203098.

3D structure databases

ProteinModelPortalP28064.
SMRP28064. Positions 73-271.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000000528.

Protein family/group databases

MEROPST01.015.

Proteomic databases

PaxDbP28064.
PRIDEP28064.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000000528; ENSRNOP00000000528; ENSRNOG00000000456.
GeneID24968.
KEGGrno:24968.
UCSCRGD:3426. rat.

Organism-specific databases

CTD5696.
RGD3426. Psmb8.

Phylogenomic databases

eggNOGCOG0638.
GeneTreeENSGT00510000046395.
HOGENOMHOG000091082.
HOVERGENHBG108297.
InParanoidP28064.
KOK02740.
OMASDLMHQY.
OrthoDBEOG7FNC86.
PhylomeDBP28064.
TreeFamTF106223.

Gene expression databases

GenevestigatorP28064.

Family and domain databases

Gene3D3.60.20.10. 1 hit.
InterProIPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamPF00227. Proteasome. 1 hit.
[Graphical view]
PRINTSPR00141. PROTEASOME.
SUPFAMSSF56235. SSF56235. 1 hit.
PROSITEPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio605008.
PROP28064.

Entry information

Entry namePSB8_RAT
AccessionPrimary (citable) accession number: P28064
Secondary accession number(s): Q6MGA4
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: December 20, 2005
Last modified: June 11, 2014
This is version 125 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries