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Protein

Proteasome subunit beta type-8

Gene

Psmb8

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Required for adipocyte differentiation (By similarity).By similarity

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei72 – 732Cleavage; by autolysisBy similarity
Active sitei73 – 731NucleophileBy similarity

GO - Molecular functioni

  1. threonine-type endopeptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. antigen processing and presentation Source: RGD
  2. fat cell differentiation Source: UniProtKB
  3. proteolysis involved in cellular protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Keywords - Biological processi

Differentiation, Immunity

Enzyme and pathway databases

ReactomeiREACT_272498. APC/C:Cdc20 mediated degradation of Securin.
REACT_274511. Regulation of ornithine decarboxylase (ODC).
REACT_275019. Ubiquitin-dependent degradation of Cyclin D1.
REACT_275816. Hh ligand biogenesis disease.
REACT_277636. Autodegradation of the E3 ubiquitin ligase COP1.
REACT_279869. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
REACT_280071. Regulation of activated PAK-2p34 by proteasome mediated degradation.
REACT_280715. GLI3 is processed to GLI3R by the proteasome.
REACT_287195. Degradation of GLI2 by the proteasome.
REACT_288207. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_289020. Degradation of beta-catenin by the destruction complex.
REACT_290448. Orc1 removal from chromatin.
REACT_295138. Cross-presentation of soluble exogenous antigens (endosomes).
REACT_297906. Separation of Sister Chromatids.
REACT_305105. degradation of DVL.
REACT_309656. SCF-beta-TrCP mediated degradation of Emi1.
REACT_312094. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_317312. SCF(Skp2)-mediated degradation of p27/p21.
REACT_318052. Activation of NF-kappaB in B cells.
REACT_325005. CDT1 association with the CDC6:ORC:origin complex.
REACT_327126. Hedgehog 'on' state.
REACT_333644. Asymmetric localization of PCP proteins.
REACT_334373. degradation of AXIN.
REACT_336450. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
REACT_336611. Hedgehog ligand biogenesis.
REACT_338477. AUF1 (hnRNP D0) destabilizes mRNA.
REACT_343414. ER-Phagosome pathway.
REACT_346077. CDK-mediated phosphorylation and removal of Cdc6.
REACT_348793. Degradation of GLI1 by the proteasome.
REACT_349901. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.

Protein family/group databases

MEROPSiT01.015.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit beta type-8 (EC:3.4.25.1)
Alternative name(s):
Macropain subunit C13
Multicatalytic endopeptidase complex subunit C13
Proteasome component C13
Proteasome subunit beta-5i
Gene namesi
Name:Psmb8
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 20

Organism-specific databases

RGDi3426. Psmb8.

Subcellular locationi

Cytoplasm PROSITE-ProRule annotation. Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. extracellular vesicular exosome Source: Ensembl
  3. nucleus Source: UniProtKB-SubCell
  4. proteasome core complex Source: UniProtKB
  5. spermatoproteasome complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 7272Removed in mature formBy similarityPRO_0000026601Add
BLAST
Chaini73 – 276204Proteasome subunit beta type-8PRO_0000026602Add
BLAST

Post-translational modificationi

Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity.By similarity

Keywords - PTMi

Zymogen

Proteomic databases

PaxDbiP28064.
PRIDEiP28064.

Expressioni

Inductioni

Up-regulated by interferon gamma (at protein level). Down-regulated by theophylline (THP), a reprotoxic agent thought to induce infertility.1 Publication

Gene expression databases

ExpressionAtlasiP28064. baseline and differential.
GenevestigatoriP28064.

Interactioni

Subunit structurei

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Component of the immunoproteasome, where it displaces the equivalent housekeeping subunit PSMB5. Component of the spermatoproteasome, a form of the proteasome specifically found in testis. Directly interacts with POMP (By similarity). Interacts with TAP1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000000528.

Structurei

3D structure databases

SMRiP28064. Positions 73-271.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0638.
GeneTreeiENSGT00510000046395.
HOGENOMiHOG000091082.
HOVERGENiHBG108297.
InParanoidiP28064.
KOiK02740.
OMAiATIRVNK.
OrthoDBiEOG7FNC86.
PhylomeDBiP28064.
TreeFamiTF106223.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
PRINTSiPR00141. PROTEASOME.
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28064-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALLDLCGAP RGQRPEWAAV DAGSGLRSDP GHYSFSVQAP ELALPRGMQP
60 70 80 90 100
TEFLRSFGDD QERKVQIEMA HGTTTLAFKF QHGVIVAVDS RASAGSYIAT
110 120 130 140 150
IRVNKVIEIN PYLLGTMSGC AADCQYWERL LAKECRLYYL RNGERISVSA
160 170 180 190 200
ASKLLSNMML QYRGMGLSMG SMICGWDKKG PGLYYVDDNG TRLSGQMFST
210 220 230 240 250
GSGNTYAYGV MDSGYRQDLS PEEAYDLARR AIVYATHRDS YSGGVVNMYH
260 270
MKKDGWVKVE STDVSDLLHK YREATL
Length:276
Mass (Da):30,570
Last modified:December 19, 2005 - v3
Checksum:i0955C80D2F969A97
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti6 – 61L → S (PubMed:1451788).Curated
Sequence conflicti16 – 249EWAAVDAGS → SGLAVDAE (PubMed:1451788).Curated
Sequence conflicti54 – 541L → S (PubMed:1451788).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX883043 Genomic DNA. Translation: CAE83942.1.
D10729 mRNA. Translation: BAA01572.1.
PIRiS21126.
RefSeqiNP_542945.2. NM_080767.2.
UniGeneiRn.203098.

Genome annotation databases

EnsembliENSRNOT00000000528; ENSRNOP00000000528; ENSRNOG00000000456.
GeneIDi24968.
KEGGirno:24968.
UCSCiRGD:3426. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX883043 Genomic DNA. Translation: CAE83942.1.
D10729 mRNA. Translation: BAA01572.1.
PIRiS21126.
RefSeqiNP_542945.2. NM_080767.2.
UniGeneiRn.203098.

3D structure databases

SMRiP28064. Positions 73-271.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000000528.

Protein family/group databases

MEROPSiT01.015.

Proteomic databases

PaxDbiP28064.
PRIDEiP28064.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000000528; ENSRNOP00000000528; ENSRNOG00000000456.
GeneIDi24968.
KEGGirno:24968.
UCSCiRGD:3426. rat.

Organism-specific databases

CTDi5696.
RGDi3426. Psmb8.

Phylogenomic databases

eggNOGiCOG0638.
GeneTreeiENSGT00510000046395.
HOGENOMiHOG000091082.
HOVERGENiHBG108297.
InParanoidiP28064.
KOiK02740.
OMAiATIRVNK.
OrthoDBiEOG7FNC86.
PhylomeDBiP28064.
TreeFamiTF106223.

Enzyme and pathway databases

ReactomeiREACT_272498. APC/C:Cdc20 mediated degradation of Securin.
REACT_274511. Regulation of ornithine decarboxylase (ODC).
REACT_275019. Ubiquitin-dependent degradation of Cyclin D1.
REACT_275816. Hh ligand biogenesis disease.
REACT_277636. Autodegradation of the E3 ubiquitin ligase COP1.
REACT_279869. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
REACT_280071. Regulation of activated PAK-2p34 by proteasome mediated degradation.
REACT_280715. GLI3 is processed to GLI3R by the proteasome.
REACT_287195. Degradation of GLI2 by the proteasome.
REACT_288207. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_289020. Degradation of beta-catenin by the destruction complex.
REACT_290448. Orc1 removal from chromatin.
REACT_295138. Cross-presentation of soluble exogenous antigens (endosomes).
REACT_297906. Separation of Sister Chromatids.
REACT_305105. degradation of DVL.
REACT_309656. SCF-beta-TrCP mediated degradation of Emi1.
REACT_312094. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_317312. SCF(Skp2)-mediated degradation of p27/p21.
REACT_318052. Activation of NF-kappaB in B cells.
REACT_325005. CDT1 association with the CDC6:ORC:origin complex.
REACT_327126. Hedgehog 'on' state.
REACT_333644. Asymmetric localization of PCP proteins.
REACT_334373. degradation of AXIN.
REACT_336450. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
REACT_336611. Hedgehog ligand biogenesis.
REACT_338477. AUF1 (hnRNP D0) destabilizes mRNA.
REACT_343414. ER-Phagosome pathway.
REACT_346077. CDK-mediated phosphorylation and removal of Cdc6.
REACT_348793. Degradation of GLI1 by the proteasome.
REACT_349901. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.

Miscellaneous databases

NextBioi605008.
PROiP28064.

Gene expression databases

ExpressionAtlasiP28064. baseline and differential.
GenevestigatoriP28064.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
PRINTSiPR00141. PROTEASOME.
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genomic sequence and comparative analysis of the rat major histocompatibility complex."
    Hurt P., Walter L., Sudbrak R., Klages S., Mueller I., Shiina T., Inoko H., Lehrach H., Guenther E., Reinhardt R., Himmelbauer H.
    Genome Res. 14:631-639(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Brown Norway.
  2. "cDNA cloning of rat proteasome subunit RC1, a homologue of RING10 located in the human MHC class II region."
    Aki M., Tamura T., Fuminori T., Iwanaga S., Kawamura Y., Shimbara N., Kagawa S., Tanaka K., Ichihara A.
    FEBS Lett. 301:65-68(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-276.
  3. "Differential expression of genes encoding constitutive and inducible 20S proteasomal core subunits in the testis and epididymis of theophylline- or 1,3-dinitrobenzene-exposed rats."
    Tengowski M.W., Feng D., Sutovsky M., Sutovsky P.
    Biol. Reprod. 76:149-163(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY THP AND DNB.

Entry informationi

Entry nameiPSB8_RAT
AccessioniPrimary (citable) accession number: P28064
Secondary accession number(s): Q6MGA4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 31, 1992
Last sequence update: December 19, 2005
Last modified: March 31, 2015
This is version 131 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Encoded in the MHC class II region.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.