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Protein

Proteasome subunit beta type-8

Gene

Psmb8

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Required for adipocyte differentiation (By similarity).By similarity

Miscellaneous

Encoded in the MHC class II region.

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei73NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Threonine protease
Biological processDifferentiation, Immunity

Enzyme and pathway databases

ReactomeiR-RNO-1169091 Activation of NF-kappaB in B cells
R-RNO-1234176 Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha
R-RNO-1236978 Cross-presentation of soluble exogenous antigens (endosomes)
R-RNO-174084 Autodegradation of Cdh1 by Cdh1:APC/C
R-RNO-174113 SCF-beta-TrCP mediated degradation of Emi1
R-RNO-174154 APC/C:Cdc20 mediated degradation of Securin
R-RNO-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
R-RNO-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-RNO-187577 SCF(Skp2)-mediated degradation of p27/p21
R-RNO-195253 Degradation of beta-catenin by the destruction complex
R-RNO-202424 Downstream TCR signaling
R-RNO-2467813 Separation of Sister Chromatids
R-RNO-2871837 FCERI mediated NF-kB activation
R-RNO-349425 Autodegradation of the E3 ubiquitin ligase COP1
R-RNO-350562 Regulation of ornithine decarboxylase (ODC)
R-RNO-382556 ABC-family proteins mediated transport
R-RNO-450408 AUF1 (hnRNP D0) binds and destabilizes mRNA
R-RNO-4608870 Asymmetric localization of PCP proteins
R-RNO-4641257 Degradation of AXIN
R-RNO-4641258 Degradation of DVL
R-RNO-5358346 Hedgehog ligand biogenesis
R-RNO-5607761 Dectin-1 mediated noncanonical NF-kB signaling
R-RNO-5607764 CLEC7A (Dectin-1) signaling
R-RNO-5610780 Degradation of GLI1 by the proteasome
R-RNO-5610785 GLI3 is processed to GLI3R by the proteasome
R-RNO-5632684 Hedgehog 'on' state
R-RNO-5658442 Regulation of RAS by GAPs
R-RNO-5668541 TNFR2 non-canonical NF-kB pathway
R-RNO-5676590 NIK-->noncanonical NF-kB signaling
R-RNO-5687128 MAPK6/MAPK4 signaling
R-RNO-5689603 UCH proteinases
R-RNO-5689880 Ub-specific processing proteases
R-RNO-68827 CDT1 association with the CDC6:ORC:origin complex
R-RNO-68949 Orc1 removal from chromatin
R-RNO-69017 CDK-mediated phosphorylation and removal of Cdc6
R-RNO-69229 Ubiquitin-dependent degradation of Cyclin D1
R-RNO-69481 G2/M Checkpoints
R-RNO-69601 Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
R-RNO-8852276 The role of GTSE1 in G2/M progression after G2 checkpoint
R-RNO-8854050 FBXL7 down-regulates AURKA during mitotic entry and in early mitosis
R-RNO-8939236 RUNX1 regulates transcription of genes involved in differentiation of HSCs
R-RNO-8939902 Regulation of RUNX2 expression and activity
R-RNO-8941858 Regulation of RUNX3 expression and activity
R-RNO-8948751 Regulation of PTEN stability and activity
R-RNO-9020702 Interleukin-1 signaling
R-RNO-983168 Antigen processing: Ubiquitination & Proteasome degradation

Protein family/group databases

MEROPSiT01.012

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit beta type-8 (EC:3.4.25.1)
Alternative name(s):
Macropain subunit C13
Multicatalytic endopeptidase complex subunit C13
Proteasome component C13
Proteasome subunit beta-5i
Gene namesi
Name:Psmb8
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 20

Organism-specific databases

RGDi3426 Psmb8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000266011 – 72Removed in mature formBy similarityAdd BLAST72
ChainiPRO_000002660273 – 276Proteasome subunit beta type-8Add BLAST204

Post-translational modificationi

Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei72 – 73Cleavage; by autolysisBy similarity2

Keywords - PTMi

Zymogen

Proteomic databases

PaxDbiP28064
PRIDEiP28064

PTM databases

PhosphoSitePlusiP28064

Expressioni

Inductioni

Up-regulated by interferon gamma (at protein level). Down-regulated by theophylline (THP), a reprotoxic agent thought to induce infertility.1 Publication

Gene expression databases

BgeeiENSRNOG00000000456
ExpressionAtlasiP28064 baseline and differential
GenevisibleiP28064 RN

Interactioni

Subunit structurei

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Component of the immunoproteasome, where it displaces the equivalent housekeeping subunit PSMB5. Component of the spermatoproteasome, a form of the proteasome specifically found in testis. Directly interacts with POMP (By similarity). Interacts with TAP1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000000528

Structurei

3D structure databases

ProteinModelPortaliP28064
SMRiP28064
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0175 Eukaryota
ENOG410XQRP LUCA
GeneTreeiENSGT00510000046395
HOGENOMiHOG000091082
HOVERGENiHBG108297
InParanoidiP28064
KOiK02740
OMAiATIRVNK
OrthoDBiEOG091G0BPS
PhylomeDBiP28064
TreeFamiTF106223

Family and domain databases

Gene3Di3.60.20.10, 1 hit
InterProiView protein in InterPro
IPR029055 Ntn_hydrolases_N
IPR000243 Pept_T1A_subB
IPR035705 Proteasome_beta8
IPR016050 Proteasome_bsu_CS
IPR001353 Proteasome_sua/b
IPR023333 Proteasome_suB-type
PANTHERiPTHR11599:SF53 PTHR11599:SF53, 1 hit
PfamiView protein in Pfam
PF00227 Proteasome, 1 hit
PRINTSiPR00141 PROTEASOME
SUPFAMiSSF56235 SSF56235, 1 hit
PROSITEiView protein in PROSITE
PS00854 PROTEASOME_BETA_1, 1 hit
PS51476 PROTEASOME_BETA_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28064-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALLDLCGAP RGQRPEWAAV DAGSGLRSDP GHYSFSVQAP ELALPRGMQP
60 70 80 90 100
TEFLRSFGDD QERKVQIEMA HGTTTLAFKF QHGVIVAVDS RASAGSYIAT
110 120 130 140 150
IRVNKVIEIN PYLLGTMSGC AADCQYWERL LAKECRLYYL RNGERISVSA
160 170 180 190 200
ASKLLSNMML QYRGMGLSMG SMICGWDKKG PGLYYVDDNG TRLSGQMFST
210 220 230 240 250
GSGNTYAYGV MDSGYRQDLS PEEAYDLARR AIVYATHRDS YSGGVVNMYH
260 270
MKKDGWVKVE STDVSDLLHK YREATL
Length:276
Mass (Da):30,570
Last modified:December 20, 2005 - v3
Checksum:i0955C80D2F969A97
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6L → S (PubMed:1451788).Curated1
Sequence conflicti16 – 24EWAAVDAGS → SGLAVDAE (PubMed:1451788).Curated9
Sequence conflicti54L → S (PubMed:1451788).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX883043 Genomic DNA Translation: CAE83942.1
D10729 mRNA Translation: BAA01572.1
PIRiS21126
RefSeqiNP_542945.2, NM_080767.2
UniGeneiRn.203098

Genome annotation databases

EnsembliENSRNOT00000000528; ENSRNOP00000000528; ENSRNOG00000000456
GeneIDi24968
KEGGirno:24968
UCSCiRGD:3426 rat

Similar proteinsi

Entry informationi

Entry nameiPSB8_RAT
AccessioniPrimary (citable) accession number: P28064
Secondary accession number(s): Q6MGA4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: December 20, 2005
Last modified: May 23, 2018
This is version 154 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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