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Protein

Proteasome subunit beta type-8

Gene

Psmb8

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Required for adipocyte differentiation (By similarity).By similarity

Miscellaneous

Encoded in the MHC class II region.

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei73NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Threonine protease
Biological processDifferentiation, Immunity

Enzyme and pathway databases

ReactomeiR-RNO-1169091. Activation of NF-kappaB in B cells.
R-RNO-1234176. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
R-RNO-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-RNO-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-RNO-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-RNO-174154. APC/C:Cdc20 mediated degradation of Securin.
R-RNO-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-RNO-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-RNO-187577. SCF(Skp2)-mediated degradation of p27/p21.
R-RNO-195253. Degradation of beta-catenin by the destruction complex.
R-RNO-202424. Downstream TCR signaling.
R-RNO-2467813. Separation of Sister Chromatids.
R-RNO-2871837. FCERI mediated NF-kB activation.
R-RNO-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-RNO-350562. Regulation of ornithine decarboxylase (ODC).
R-RNO-382556. ABC-family proteins mediated transport.
R-RNO-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-RNO-4608870. Asymmetric localization of PCP proteins.
R-RNO-4641257. Degradation of AXIN.
R-RNO-4641258. Degradation of DVL.
R-RNO-5358346. Hedgehog ligand biogenesis.
R-RNO-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-RNO-5607764. CLEC7A (Dectin-1) signaling.
R-RNO-5610780. Degradation of GLI1 by the proteasome.
R-RNO-5610785. GLI3 is processed to GLI3R by the proteasome.
R-RNO-5632684. Hedgehog 'on' state.
R-RNO-5658442. Regulation of RAS by GAPs.
R-RNO-5668541. TNFR2 non-canonical NF-kB pathway.
R-RNO-5676590. NIK-->noncanonical NF-kB signaling.
R-RNO-5687128. MAPK6/MAPK4 signaling.
R-RNO-5689603. UCH proteinases.
R-RNO-5689880. Ub-specific processing proteases.
R-RNO-68827. CDT1 association with the CDC6:ORC:origin complex.
R-RNO-68949. Orc1 removal from chromatin.
R-RNO-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-RNO-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-RNO-69481. G2/M Checkpoints.
R-RNO-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-RNO-8852276. The role of GTSE1 in G2/M progression after G2 checkpoint.
R-RNO-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-RNO-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Protein family/group databases

MEROPSiT01.012.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit beta type-8 (EC:3.4.25.1)
Alternative name(s):
Macropain subunit C13
Multicatalytic endopeptidase complex subunit C13
Proteasome component C13
Proteasome subunit beta-5i
Gene namesi
Name:Psmb8
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 20

Organism-specific databases

RGDi3426. Psmb8.

Subcellular locationi

  • Cytoplasm PROSITE-ProRule annotation
  • Nucleus By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000266011 – 72Removed in mature formBy similarityAdd BLAST72
ChainiPRO_000002660273 – 276Proteasome subunit beta type-8Add BLAST204

Post-translational modificationi

Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei72 – 73Cleavage; by autolysisBy similarity2

Keywords - PTMi

Zymogen

Proteomic databases

PaxDbiP28064.
PRIDEiP28064.

PTM databases

PhosphoSitePlusiP28064.

Expressioni

Inductioni

Up-regulated by interferon gamma (at protein level). Down-regulated by theophylline (THP), a reprotoxic agent thought to induce infertility.1 Publication

Gene expression databases

BgeeiENSRNOG00000000456.
ExpressionAtlasiP28064. baseline and differential.
GenevisibleiP28064. RN.

Interactioni

Subunit structurei

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Component of the immunoproteasome, where it displaces the equivalent housekeeping subunit PSMB5. Component of the spermatoproteasome, a form of the proteasome specifically found in testis. Directly interacts with POMP (By similarity). Interacts with TAP1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000000528.

Structurei

3D structure databases

ProteinModelPortaliP28064.
SMRiP28064.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0175. Eukaryota.
ENOG410XQRP. LUCA.
GeneTreeiENSGT00510000046395.
HOGENOMiHOG000091082.
HOVERGENiHBG108297.
InParanoidiP28064.
KOiK02740.
OMAiATIRVNK.
OrthoDBiEOG091G0BPS.
PhylomeDBiP28064.
TreeFamiTF106223.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiView protein in InterPro
IPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
PfamiView protein in Pfam
PF00227. Proteasome. 1 hit.
PRINTSiPR00141. PROTEASOME.
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiView protein in PROSITE
PS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28064-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALLDLCGAP RGQRPEWAAV DAGSGLRSDP GHYSFSVQAP ELALPRGMQP
60 70 80 90 100
TEFLRSFGDD QERKVQIEMA HGTTTLAFKF QHGVIVAVDS RASAGSYIAT
110 120 130 140 150
IRVNKVIEIN PYLLGTMSGC AADCQYWERL LAKECRLYYL RNGERISVSA
160 170 180 190 200
ASKLLSNMML QYRGMGLSMG SMICGWDKKG PGLYYVDDNG TRLSGQMFST
210 220 230 240 250
GSGNTYAYGV MDSGYRQDLS PEEAYDLARR AIVYATHRDS YSGGVVNMYH
260 270
MKKDGWVKVE STDVSDLLHK YREATL
Length:276
Mass (Da):30,570
Last modified:December 20, 2005 - v3
Checksum:i0955C80D2F969A97
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6L → S (PubMed:1451788).Curated1
Sequence conflicti16 – 24EWAAVDAGS → SGLAVDAE (PubMed:1451788).Curated9
Sequence conflicti54L → S (PubMed:1451788).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX883043 Genomic DNA. Translation: CAE83942.1.
D10729 mRNA. Translation: BAA01572.1.
PIRiS21126.
RefSeqiNP_542945.2. NM_080767.2.
UniGeneiRn.203098.

Genome annotation databases

EnsembliENSRNOT00000000528; ENSRNOP00000000528; ENSRNOG00000000456.
GeneIDi24968.
KEGGirno:24968.
UCSCiRGD:3426. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX883043 Genomic DNA. Translation: CAE83942.1.
D10729 mRNA. Translation: BAA01572.1.
PIRiS21126.
RefSeqiNP_542945.2. NM_080767.2.
UniGeneiRn.203098.

3D structure databases

ProteinModelPortaliP28064.
SMRiP28064.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000000528.

Protein family/group databases

MEROPSiT01.012.

PTM databases

PhosphoSitePlusiP28064.

Proteomic databases

PaxDbiP28064.
PRIDEiP28064.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000000528; ENSRNOP00000000528; ENSRNOG00000000456.
GeneIDi24968.
KEGGirno:24968.
UCSCiRGD:3426. rat.

Organism-specific databases

CTDi5696.
RGDi3426. Psmb8.

Phylogenomic databases

eggNOGiKOG0175. Eukaryota.
ENOG410XQRP. LUCA.
GeneTreeiENSGT00510000046395.
HOGENOMiHOG000091082.
HOVERGENiHBG108297.
InParanoidiP28064.
KOiK02740.
OMAiATIRVNK.
OrthoDBiEOG091G0BPS.
PhylomeDBiP28064.
TreeFamiTF106223.

Enzyme and pathway databases

ReactomeiR-RNO-1169091. Activation of NF-kappaB in B cells.
R-RNO-1234176. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
R-RNO-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-RNO-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-RNO-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-RNO-174154. APC/C:Cdc20 mediated degradation of Securin.
R-RNO-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-RNO-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-RNO-187577. SCF(Skp2)-mediated degradation of p27/p21.
R-RNO-195253. Degradation of beta-catenin by the destruction complex.
R-RNO-202424. Downstream TCR signaling.
R-RNO-2467813. Separation of Sister Chromatids.
R-RNO-2871837. FCERI mediated NF-kB activation.
R-RNO-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-RNO-350562. Regulation of ornithine decarboxylase (ODC).
R-RNO-382556. ABC-family proteins mediated transport.
R-RNO-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-RNO-4608870. Asymmetric localization of PCP proteins.
R-RNO-4641257. Degradation of AXIN.
R-RNO-4641258. Degradation of DVL.
R-RNO-5358346. Hedgehog ligand biogenesis.
R-RNO-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-RNO-5607764. CLEC7A (Dectin-1) signaling.
R-RNO-5610780. Degradation of GLI1 by the proteasome.
R-RNO-5610785. GLI3 is processed to GLI3R by the proteasome.
R-RNO-5632684. Hedgehog 'on' state.
R-RNO-5658442. Regulation of RAS by GAPs.
R-RNO-5668541. TNFR2 non-canonical NF-kB pathway.
R-RNO-5676590. NIK-->noncanonical NF-kB signaling.
R-RNO-5687128. MAPK6/MAPK4 signaling.
R-RNO-5689603. UCH proteinases.
R-RNO-5689880. Ub-specific processing proteases.
R-RNO-68827. CDT1 association with the CDC6:ORC:origin complex.
R-RNO-68949. Orc1 removal from chromatin.
R-RNO-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-RNO-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-RNO-69481. G2/M Checkpoints.
R-RNO-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-RNO-8852276. The role of GTSE1 in G2/M progression after G2 checkpoint.
R-RNO-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-RNO-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

PROiPR:P28064.

Gene expression databases

BgeeiENSRNOG00000000456.
ExpressionAtlasiP28064. baseline and differential.
GenevisibleiP28064. RN.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiView protein in InterPro
IPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
PfamiView protein in Pfam
PF00227. Proteasome. 1 hit.
PRINTSiPR00141. PROTEASOME.
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiView protein in PROSITE
PS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPSB8_RAT
AccessioniPrimary (citable) accession number: P28064
Secondary accession number(s): Q6MGA4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: December 20, 2005
Last modified: May 10, 2017
This is version 147 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.