P28047 (WNT7B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein Wnt-7b

Gene names

Name:	Wnt7b
Synonyms:	Wnt-7b

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
Subunit structure	Interacts with PORCN. Ref.3
Subcellular location	Secreted › extracellular space › extracellular matrix.
Post-translational modification	Palmitoylation at Ser-206 is required for efficient binding to frizzled receptors. It is also required for subsequent palmitoylation at Cys-73. Palmitoylation is necessary for proper trafficking to cell surface By similarity.
Sequence similarities	Belongs to the Wnt family.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Cellular component	Extracellular matrix Secreted
Domain	Signal
Molecular function	Developmental protein
PTM	Glycoprotein Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	activation of JUN kinase activity Inferred from Biological aspect of Ancestor. Source: RefGenome anatomical structure regression Inferred from mutant phenotype PubMed 16163358. Source: MGI anterior/posterior pattern specification Inferred from Biological aspect of Ancestor. Source: RefGenome axis specification Inferred from Biological aspect of Ancestor. Source: RefGenome axonogenesis Inferred from Biological aspect of Ancestor. Source: RefGenome canonical Wnt receptor signaling pathway Inferred from direct assay PubMed 17804636. Source: MGI cell proliferation in forebrain Inferred from direct assay PubMed 12843296. Source: BHF-UCL cell-cell signaling Traceable author statement PubMed 9889131. Source: MGI central nervous system vasculogenesis Inferred from genetic interaction PubMed 19023080. Source: MGI chorio-allantoic fusion Inferred from mutant phenotype PubMed 11543617. Source: MGI developmental growth involved in morphogenesis Inferred from mutant phenotype PubMed 19060336. Source: MGI establishment or maintenance of polarity of embryonic epithelium Inferred from mutant phenotype PubMed 19060336. Source: MGI forebrain regionalization Inferred from expression pattern PubMed 15164427. Source: UniProtKB induction of apoptosis Inferred from mutant phenotype PubMed 16163358. Source: MGI inner medullary collecting duct development Inferred from mutant phenotype PubMed 19060336. Source: MGI lobar bronchus development Inferred from mutant phenotype PubMed 18367557. Source: MGI lung epithelium development Inferred from mutant phenotype PubMed 18367557. Source: MGI lung morphogenesis Inferred from mutant phenotype PubMed 18367557. Source: MGI lung-associated mesenchyme development Inferred from mutant phenotype PubMed 18367557. Source: MGI metanephric collecting duct development Inferred from mutant phenotype PubMed 19060336. Source: MGI metanephric loop of Henle development Inferred from mutant phenotype PubMed 19060336. Source: MGI metanephros morphogenesis Inferred from mutant phenotype PubMed 19060336. Source: MGI negative regulation of neurogenesis Inferred from direct assay PubMed 12843296. Source: BHF-UCL negative regulation of smoothened signaling pathway Inferred from mutant phenotype PubMed 10781055. Source: MGI negative regulation of transcription from RNA polymerase II promoter Inferred from Biological aspect of Ancestor. Source: RefGenome odontogenesis of dentin-containing tooth Inferred from mutant phenotype PubMed 10781055. Source: MGI outer medullary collecting duct development Inferred from mutant phenotype PubMed 19060336. Source: MGI oxygen homeostasis Inferred from mutant phenotype PubMed 18367557. Source: MGI positive regulation of JNK cascade Inferred from Biological aspect of Ancestor. Source: RefGenome positive regulation of cell proliferation Inferred from mutant phenotype PubMed 19690384. Source: MGI positive regulation of osteoblast differentiation Inferred from direct assay PubMed 15576404. Source: MGI positive regulation of transcription, DNA-dependent Inferred from Biological aspect of Ancestor. Source: RefGenome renal inner medulla development Inferred from mutant phenotype PubMed 19060336. Source: MGI renal outer medulla development Inferred from mutant phenotype PubMed 19060336. Source: MGI smooth muscle cell differentiation Inferred from mutant phenotype PubMed 19690384. Source: MGI stem cell development Inferred from direct assay PubMed 12843296. Source: BHF-UCL synapse organization Inferred from genetic interaction PubMed 16818724. Source: MGI trachea cartilage morphogenesis Inferred from mutant phenotype PubMed 18367557. Source: MGI
Cellular_component	cell surface Inferred from direct assay PubMed 8167409. Source: BHF-UCL extracellular space Inferred from Biological aspect of Ancestor. Source: RefGenome plasma membrane Inferred from Biological aspect of Ancestor. Source: RefGenome proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	receptor agonist activity Inferred by curator PubMed 16163358. Source: BHF-UCL
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 349	325	Protein Wnt-7b		PRO_0000041445
Amino acid modifications
	Lipidation	73	1	S-palmitoyl cysteine By similarity
	Lipidation	206	1	O-palmitoyl serine; by PORCN By similarity
	Glycosylation	83	1	N-linked (GlcNAc...) Potential
	Glycosylation	127	1	N-linked (GlcNAc...) Potential
	Glycosylation	295	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	33	1	G → V in AAH52018. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P28047 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: BDD82AB020DC680E	FASTA	349	39,302
	10 20 30 40 50 60 MHRNFRKWIF YVFLCFGVLY VKLGALSSVV ALGANIICNK IPGLAPRQRA ICQSRPDAII 70 80 90 100 110 120 VIGEGAQMGI DECQHQFRFG RWNCSALGEK TVFGQELRVG SREAAFTYAI TAAGVAHAVT 130 140 150 160 170 180 AACSQGNLSN CGCDREKQGY YNQAEGWKWG GCSADVRYGI DFSRRFVDAR EIKKNARRLM 190 200 210 220 230 240 NLHNNEAGRK VLEDRMKLEC KCHGVSGSCT TKTCWTTLPK FREVGHLLKE KYNAAVQVEV 250 260 270 280 290 300 VRASRLRQPT FLRIKQLRSY QKPMETDLVY IEKSPNYCEE DAATGSVGTQ GRLCNRTSPG 310 320 330 340 ADGCDTMCCG RGYNTHQYTK VWQCNCKFHW CCFVKCNTCS ERTEVFTCK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression of multiple novel Wnt-1/int-1-related genes during fetal and adult mouse development." Gavin B.J., McMahon J.A., McMahon A.P. Genes Dev. 4:2319-2332(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6.
[3]	"The evolutionarily conserved porcupine gene family is involved in the processing of the Wnt family." Tanaka K., Okabayashi H., Asashima M., Perrimon N., Kadowaki T. Eur. J. Biochem. 267:4300-4311(2000) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PORCN.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M89802 mRNA. Translation: AAA40571.1. BC052018 mRNA. Translation: AAH52018.2.
IPI	IPI00116644.
PIR	H36470.
RefSeq	NP_001157105.1. NM_001163633.1. NP_001157106.1. NM_001163634.1. NP_033554.3. NM_009528.3.
UniGene	Mm.306946.
3D structure databases
ProteinModelPortal	P28047.
SMR	P28047. Positions 62-341.
ModBase	Search...
PTM databases
PhosphoSite	P28047.
Proteomic databases
PRIDE	P28047.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000023015; ENSMUSP00000023015; ENSMUSG00000022382.
GeneID	22422.
KEGG	mmu:22422.
UCSC	uc007xdf.2. mouse.
Organism-specific databases
CTD	7477.
MGI	MGI:98962. Wnt7b.
Phylogenomic databases
eggNOG	NOG284879.
GeneTree	ENSGT00700000104317.
HOGENOM	HOG000039528.
HOVERGEN	HBG001595.
KO	K00572.
OrthoDB	EOG4GXFMZ.
Gene expression databases
ArrayExpress	P28047.
Bgee	P28047.
CleanEx	MM_WNT7B.
Genevestigator	P28047.
Family and domain databases
InterPro	IPR005817. Wnt. IPR013300. Wnt7. IPR018161. Wnt_CS. [Graphical view]
PANTHER	PTHR12027. PTHR12027. 1 hit. PTHR12027:SF22. PTHR12027:SF22. 1 hit.
Pfam	PF00110. wnt. 1 hit. [Graphical view]
PRINTS	PR01891. WNT7PROTEIN. PR01349. WNTPROTEIN.
SMART	SM00097. WNT1. 1 hit. [Graphical view]
PROSITE	PS00246. WNT1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	302857.
SOURCE	Search...

Entry information

Entry name

WNT7B_MOUSE

Accession

Primary (citable) accession number: P28047
Secondary accession number(s): Q80US5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	May 1, 2013
This is version 111 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program