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P28039

- AOAH_HUMAN

UniProt

P28039 - AOAH_HUMAN

Protein

Acyloxyacyl hydrolase

Gene

AOAH

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 1 (01 Aug 1992)
      Previous versions | rss
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    Functioni

    Removes the secondary (acyloxyacyl-linked) fatty acyl chains from the lipid A region of bacterial lipopolysaccharides.

    Catalytic activityi

    3-(acyloxy)acyl group of bacterial toxin = 3-hydroxyacyl group of bacterial toxin + a fatty acid.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei263 – 2631Sequence Analysis

    GO - Molecular functioni

    1. acyloxyacyl hydrolase activity Source: UniProtKB-EC
    2. catalytic activity Source: ProtInc
    3. lipoprotein lipase activity Source: ProtInc

    GO - Biological processi

    1. inflammatory response Source: ProtInc
    2. lipid metabolic process Source: ProtInc
    3. lipopolysaccharide metabolic process Source: Ensembl
    4. negative regulation of inflammatory response Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase

    Enzyme and pathway databases

    BRENDAi3.1.1.77. 2681.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acyloxyacyl hydrolase (EC:3.1.1.77)
    Cleaved into the following 2 chains:
    Gene namesi
    Name:AOAH
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 7

    Organism-specific databases

    HGNCiHGNC:548. AOAH.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA24838.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Propeptidei24 – 34111 PublicationPRO_0000020739Add
    BLAST
    Chaini35 – 156122Acyloxyacyl hydrolase small subunitPRO_0000020740Add
    BLAST
    Chaini157 – 575419Acyloxyacyl hydrolase large subunitPRO_0000020741Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi41 ↔ 114PROSITE-ProRule annotation
    Disulfide bondi44 ↔ 108PROSITE-ProRule annotation
    Glycosylationi59 – 591N-linked (GlcNAc...)PROSITE-ProRule annotation
    Disulfide bondi70 ↔ 83PROSITE-ProRule annotation
    Disulfide bondi123 ↔ ?
    Glycosylationi207 – 2071N-linked (GlcNAc...)PROSITE-ProRule annotation
    Glycosylationi336 – 3361N-linked (GlcNAc...)PROSITE-ProRule annotation
    Glycosylationi409 – 4091N-linked (GlcNAc...)PROSITE-ProRule annotation
    Glycosylationi466 – 4661N-linked (GlcNAc...)PROSITE-ProRule annotation

    Post-translational modificationi

    Both subunits contain a number of cysteine residues that may form disulfide bridges.

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Zymogen

    Proteomic databases

    PaxDbiP28039.
    PRIDEiP28039.

    PTM databases

    PhosphoSiteiP28039.

    Expressioni

    Gene expression databases

    ArrayExpressiP28039.
    BgeeiP28039.
    CleanExiHS_AOAH.
    GenevestigatoriP28039.

    Organism-specific databases

    HPAiHPA021666.
    HPA026714.
    HPA026716.

    Interactioni

    Subunit structurei

    Heterodimer.

    Protein-protein interaction databases

    IntActiP28039. 1 interaction.
    STRINGi9606.ENSP00000258749.

    Structurei

    3D structure databases

    ProteinModelPortaliP28039.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini37 – 11882Saposin B-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 saposin B-type domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG47076.
    HOGENOMiHOG000008100.
    HOVERGENiHBG004254.
    InParanoidiP28039.
    KOiK01065.
    OrthoDBiEOG75MVVQ.
    PhylomeDBiP28039.
    TreeFamiTF329246.

    Family and domain databases

    Gene3Di1.10.225.10. 1 hit.
    3.40.50.1110. 2 hits.
    InterProiIPR001087. Lipase_GDSL.
    IPR008138. SapB_2.
    IPR011001. Saposin-like.
    IPR008139. SaposinB.
    IPR013831. SGNH_hydro-type_esterase_dom.
    [Graphical view]
    PfamiPF00657. Lipase_GDSL. 1 hit.
    PF03489. SapB_2. 1 hit.
    [Graphical view]
    SMARTiSM00741. SapB. 1 hit.
    [Graphical view]
    SUPFAMiSSF47862. SSF47862. 1 hit.
    PROSITEiPS50015. SAP_B. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P28039-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MQSPWKILTV APLFLLLSLQ SSASPANDDQ SRPSLSNGHT CVGCVLVVSV    50
    IEQLAQVHNS TVQASMERLC SYLPEKLFLK TTCYLVIDKF GSDIIKLLSA 100
    DMNADVVCHT LEFCKQNTGQ PLCHLYPLPK ETWKFTLQKA RQIVKKSPIL 150
    KYSRSGSDIC SLPVLAKICQ KIKLAMEQSV PFKDVDSDKY SVFPTLRGYH 200
    WRGRDCNDSD ESVYPGRRPN NWDVHQDSNC NGIWGVDPKD GVPYEKKFCE 250
    GSQPRGIILL GDSAGAHFHI SPEWITASQM SLNSFINLPT ALTNELDWPQ 300
    LSGATGFLDS TVGIKEKSIY LRLWKRNHCN HRDYQNISRN GASSRNLKKF 350
    IESLSRNKVL DYPAIVIYAM IGNDVCSGKS DPVPAMTTPE KLYSNVMQTL 400
    KHLNSHLPNG SHVILYGLPD GTFLWDNLHN RYHPLGQLNK DMTYAQLYSF 450
    LNCLQVSPCH GWMSSNKTLR TLTSERAEQL SNTLKKIAAS EKFTNFNLFY 500
    MDFAFHEIIQ EWQKRGGQPW QLIEPVDGFH PNEVALLLLA DHFWKKVQLQ 550
    WPQILGKENP FNPQIKQVFG DQGGH 575
    Length:575
    Mass (Da):65,105
    Last modified:August 1, 1992 - v1
    Checksum:iE3B3853DBD308AF7
    GO
    Isoform 2 (identifier: P28039-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         43-74: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:543
    Mass (Da):61,647
    Checksum:iBE14624794189A57
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti222 – 2221W → C in BAD97196. 1 PublicationCurated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti28 – 281D → N.2 Publications
    Corresponds to variant rs11976480 [ dbSNP | Ensembl ].
    VAR_050663
    Natural varianti166 – 1661A → T.
    Corresponds to variant rs3735384 [ dbSNP | Ensembl ].
    VAR_020133
    Natural varianti266 – 2661A → G.
    Corresponds to variant rs3735386 [ dbSNP | Ensembl ].
    VAR_033513

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei43 – 7432Missing in isoform 2. 1 PublicationVSP_042571Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M62840 mRNA. Translation: AAA35506.1.
    AK297016 mRNA. Translation: BAH12476.1.
    AK223476 mRNA. Translation: BAD97196.1.
    AC083876 Genomic DNA. No translation available.
    AC087069 Genomic DNA. No translation available.
    CH236951 Genomic DNA. Translation: EAL23977.1.
    CH471073 Genomic DNA. Translation: EAW94073.1.
    BC025698 mRNA. Translation: AAH25698.1.
    CCDSiCCDS5448.1. [P28039-1]
    CCDS55102.1. [P28039-2]
    PIRiA40292.
    RefSeqiNP_001170977.1. NM_001177506.1.
    NP_001170978.1. NM_001177507.1. [P28039-2]
    NP_001628.1. NM_001637.3. [P28039-1]
    UniGeneiHs.488007.

    Genome annotation databases

    EnsembliENST00000258749; ENSP00000258749; ENSG00000136250. [P28039-1]
    ENST00000535891; ENSP00000441101; ENSG00000136250. [P28039-2]
    GeneIDi313.
    KEGGihsa:313.
    UCSCiuc003tfh.4. human. [P28039-1]
    uc011kba.2. human. [P28039-2]

    Polymorphism databases

    DMDMi113976.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M62840 mRNA. Translation: AAA35506.1 .
    AK297016 mRNA. Translation: BAH12476.1 .
    AK223476 mRNA. Translation: BAD97196.1 .
    AC083876 Genomic DNA. No translation available.
    AC087069 Genomic DNA. No translation available.
    CH236951 Genomic DNA. Translation: EAL23977.1 .
    CH471073 Genomic DNA. Translation: EAW94073.1 .
    BC025698 mRNA. Translation: AAH25698.1 .
    CCDSi CCDS5448.1. [P28039-1 ]
    CCDS55102.1. [P28039-2 ]
    PIRi A40292.
    RefSeqi NP_001170977.1. NM_001177506.1.
    NP_001170978.1. NM_001177507.1. [P28039-2 ]
    NP_001628.1. NM_001637.3. [P28039-1 ]
    UniGenei Hs.488007.

    3D structure databases

    ProteinModelPortali P28039.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P28039. 1 interaction.
    STRINGi 9606.ENSP00000258749.

    PTM databases

    PhosphoSitei P28039.

    Polymorphism databases

    DMDMi 113976.

    Proteomic databases

    PaxDbi P28039.
    PRIDEi P28039.

    Protocols and materials databases

    DNASUi 313.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000258749 ; ENSP00000258749 ; ENSG00000136250 . [P28039-1 ]
    ENST00000535891 ; ENSP00000441101 ; ENSG00000136250 . [P28039-2 ]
    GeneIDi 313.
    KEGGi hsa:313.
    UCSCi uc003tfh.4. human. [P28039-1 ]
    uc011kba.2. human. [P28039-2 ]

    Organism-specific databases

    CTDi 313.
    GeneCardsi GC07M036519.
    HGNCi HGNC:548. AOAH.
    HPAi HPA021666.
    HPA026714.
    HPA026716.
    MIMi 102593. gene.
    neXtProti NX_P28039.
    PharmGKBi PA24838.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG47076.
    HOGENOMi HOG000008100.
    HOVERGENi HBG004254.
    InParanoidi P28039.
    KOi K01065.
    OrthoDBi EOG75MVVQ.
    PhylomeDBi P28039.
    TreeFami TF329246.

    Enzyme and pathway databases

    BRENDAi 3.1.1.77. 2681.

    Miscellaneous databases

    ChiTaRSi AOAH. human.
    GeneWikii AOAH.
    GenomeRNAii 313.
    NextBioi 1273.
    PROi P28039.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P28039.
    Bgeei P28039.
    CleanExi HS_AOAH.
    Genevestigatori P28039.

    Family and domain databases

    Gene3Di 1.10.225.10. 1 hit.
    3.40.50.1110. 2 hits.
    InterProi IPR001087. Lipase_GDSL.
    IPR008138. SapB_2.
    IPR011001. Saposin-like.
    IPR008139. SaposinB.
    IPR013831. SGNH_hydro-type_esterase_dom.
    [Graphical view ]
    Pfami PF00657. Lipase_GDSL. 1 hit.
    PF03489. SapB_2. 1 hit.
    [Graphical view ]
    SMARTi SM00741. SapB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47862. SSF47862. 1 hit.
    PROSITEi PS50015. SAP_B. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Expression and characterization of recombinant human acyloxyacyl hydrolase, a leukocyte enzyme that deacylates bacterial lipopolysaccharides."
      Hagen F.S., Grant F.J., Kuijper J.L., Slaughter C.A., Moomaw C.R., Orth K., O'Hara P.J., Munford R.S.
      Biochemistry 30:8415-8423(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 35-49 AND 157-185.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Umbilical cord blood.
    3. Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
      Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Synovial cell.
    4. "The DNA sequence of human chromosome 7."
      Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L.
      , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
      Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "Human chromosome 7: DNA sequence and biology."
      Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S.
      , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C.
      Science 300:767-772(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ASN-28.
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ASN-28.
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Pancreas.

    Entry informationi

    Entry nameiAOAH_HUMAN
    AccessioniPrimary (citable) accession number: P28039
    Secondary accession number(s): A4D1Y5, B7Z490, Q53F13
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: August 1, 1992
    Last modified: October 1, 2014
    This is version 127 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 7
      Human chromosome 7: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3