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Protein

Acyloxyacyl hydrolase

Gene

AOAH

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Removes the secondary (acyloxyacyl-linked) fatty acyl chains from the lipid A region of bacterial lipopolysaccharides.

Catalytic activityi

3-(acyloxy)acyl group of bacterial toxin = 3-hydroxyacyl group of bacterial toxin + a fatty acid.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei263Sequence analysis1

GO - Molecular functioni

  • acyloxyacyl hydrolase activity Source: UniProtKB-EC
  • catalytic activity Source: ProtInc
  • lipoprotein lipase activity Source: ProtInc

GO - Biological processi

  • inflammatory response Source: ProtInc
  • lipid metabolic process Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciZFISH:HS06138-MONOMER.
BRENDAi3.1.1.77. 2681.

Names & Taxonomyi

Protein namesi
Recommended name:
Acyloxyacyl hydrolase (EC:3.1.1.77)
Cleaved into the following 2 chains:
Gene namesi
Name:AOAH
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:548. AOAH.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi313.
OpenTargetsiENSG00000136250.
PharmGKBiPA24838.

Chemistry databases

GuidetoPHARMACOLOGYi2873.

Polymorphism and mutation databases

BioMutaiAOAH.
DMDMi113976.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
PropeptideiPRO_000002073924 – 341 PublicationAdd BLAST11
ChainiPRO_000002074035 – 156Acyloxyacyl hydrolase small subunitAdd BLAST122
ChainiPRO_0000020741157 – 575Acyloxyacyl hydrolase large subunitAdd BLAST419

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi41 ↔ 114PROSITE-ProRule annotation
Disulfide bondi44 ↔ 108PROSITE-ProRule annotation
Glycosylationi59N-linked (GlcNAc...)PROSITE-ProRule annotation1
Disulfide bondi70 ↔ 83PROSITE-ProRule annotation
Disulfide bondi123 ↔ ?
Glycosylationi207N-linked (GlcNAc...)PROSITE-ProRule annotation1
Glycosylationi336N-linked (GlcNAc...)PROSITE-ProRule annotation1
Glycosylationi409N-linked (GlcNAc...)PROSITE-ProRule annotation1
Glycosylationi466N-linked (GlcNAc...)PROSITE-ProRule annotation1

Post-translational modificationi

Both subunits contain a number of cysteine residues that may form disulfide bridges.

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

EPDiP28039.
PaxDbiP28039.
PeptideAtlasiP28039.
PRIDEiP28039.

PTM databases

iPTMnetiP28039.
PhosphoSitePlusiP28039.

Expressioni

Gene expression databases

BgeeiENSG00000136250.
CleanExiHS_AOAH.
ExpressionAtlasiP28039. baseline and differential.
GenevisibleiP28039. HS.

Organism-specific databases

HPAiHPA021666.
HPA026714.
HPA026716.

Interactioni

Subunit structurei

Heterodimer.

Protein-protein interaction databases

IntActiP28039. 1 interactor.
STRINGi9606.ENSP00000258749.

Structurei

3D structure databases

ProteinModelPortaliP28039.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini37 – 118Saposin B-typePROSITE-ProRule annotationAdd BLAST82

Sequence similaritiesi

Contains 1 saposin B-type domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IFCC. Eukaryota.
ENOG410XTCS. LUCA.
GeneTreeiENSGT00390000008427.
HOGENOMiHOG000008100.
HOVERGENiHBG004254.
InParanoidiP28039.
KOiK01065.
PhylomeDBiP28039.
TreeFamiTF329246.

Family and domain databases

Gene3Di1.10.225.10. 1 hit.
3.40.50.1110. 2 hits.
InterProiIPR001087. GDSL.
IPR008138. SapB_2.
IPR011001. Saposin-like.
IPR008139. SaposinB_dom.
IPR013830. SGNH_hydro.
[Graphical view]
PfamiPF00657. Lipase_GDSL. 1 hit.
PF03489. SapB_2. 1 hit.
[Graphical view]
SMARTiSM00741. SapB. 1 hit.
[Graphical view]
SUPFAMiSSF47862. SSF47862. 1 hit.
SSF52266. SSF52266. 2 hits.
PROSITEiPS50015. SAP_B. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P28039-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQSPWKILTV APLFLLLSLQ SSASPANDDQ SRPSLSNGHT CVGCVLVVSV
60 70 80 90 100
IEQLAQVHNS TVQASMERLC SYLPEKLFLK TTCYLVIDKF GSDIIKLLSA
110 120 130 140 150
DMNADVVCHT LEFCKQNTGQ PLCHLYPLPK ETWKFTLQKA RQIVKKSPIL
160 170 180 190 200
KYSRSGSDIC SLPVLAKICQ KIKLAMEQSV PFKDVDSDKY SVFPTLRGYH
210 220 230 240 250
WRGRDCNDSD ESVYPGRRPN NWDVHQDSNC NGIWGVDPKD GVPYEKKFCE
260 270 280 290 300
GSQPRGIILL GDSAGAHFHI SPEWITASQM SLNSFINLPT ALTNELDWPQ
310 320 330 340 350
LSGATGFLDS TVGIKEKSIY LRLWKRNHCN HRDYQNISRN GASSRNLKKF
360 370 380 390 400
IESLSRNKVL DYPAIVIYAM IGNDVCSGKS DPVPAMTTPE KLYSNVMQTL
410 420 430 440 450
KHLNSHLPNG SHVILYGLPD GTFLWDNLHN RYHPLGQLNK DMTYAQLYSF
460 470 480 490 500
LNCLQVSPCH GWMSSNKTLR TLTSERAEQL SNTLKKIAAS EKFTNFNLFY
510 520 530 540 550
MDFAFHEIIQ EWQKRGGQPW QLIEPVDGFH PNEVALLLLA DHFWKKVQLQ
560 570
WPQILGKENP FNPQIKQVFG DQGGH
Length:575
Mass (Da):65,105
Last modified:August 1, 1992 - v1
Checksum:iE3B3853DBD308AF7
GO
Isoform 2 (identifier: P28039-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     43-74: Missing.

Note: No experimental confirmation available.
Show »
Length:543
Mass (Da):61,647
Checksum:iBE14624794189A57
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti222W → C in BAD97196 (Ref. 6) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05066328D → N.2 PublicationsCorresponds to variant rs11976480dbSNPEnsembl.1
Natural variantiVAR_020133166A → T.Corresponds to variant rs3735384dbSNPEnsembl.1
Natural variantiVAR_033513266A → G.Corresponds to variant rs3735386dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04257143 – 74Missing in isoform 2. 1 PublicationAdd BLAST32

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62840 mRNA. Translation: AAA35506.1.
AK297016 mRNA. Translation: BAH12476.1.
AK223476 mRNA. Translation: BAD97196.1.
AC083876 Genomic DNA. No translation available.
AC087069 Genomic DNA. No translation available.
CH236951 Genomic DNA. Translation: EAL23977.1.
CH471073 Genomic DNA. Translation: EAW94073.1.
BC025698 mRNA. Translation: AAH25698.1.
CCDSiCCDS5448.1. [P28039-1]
CCDS55102.1. [P28039-2]
PIRiA40292.
RefSeqiNP_001170977.1. NM_001177506.1.
NP_001170978.1. NM_001177507.1. [P28039-2]
NP_001628.1. NM_001637.3. [P28039-1]
UniGeneiHs.488007.
Hs.689297.

Genome annotation databases

EnsembliENST00000612871; ENSP00000484305; ENSG00000136250. [P28039-2]
ENST00000617537; ENSP00000483783; ENSG00000136250. [P28039-1]
GeneIDi313.
KEGGihsa:313.
UCSCiuc032zjw.2. human. [P28039-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62840 mRNA. Translation: AAA35506.1.
AK297016 mRNA. Translation: BAH12476.1.
AK223476 mRNA. Translation: BAD97196.1.
AC083876 Genomic DNA. No translation available.
AC087069 Genomic DNA. No translation available.
CH236951 Genomic DNA. Translation: EAL23977.1.
CH471073 Genomic DNA. Translation: EAW94073.1.
BC025698 mRNA. Translation: AAH25698.1.
CCDSiCCDS5448.1. [P28039-1]
CCDS55102.1. [P28039-2]
PIRiA40292.
RefSeqiNP_001170977.1. NM_001177506.1.
NP_001170978.1. NM_001177507.1. [P28039-2]
NP_001628.1. NM_001637.3. [P28039-1]
UniGeneiHs.488007.
Hs.689297.

3D structure databases

ProteinModelPortaliP28039.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP28039. 1 interactor.
STRINGi9606.ENSP00000258749.

Chemistry databases

GuidetoPHARMACOLOGYi2873.

PTM databases

iPTMnetiP28039.
PhosphoSitePlusiP28039.

Polymorphism and mutation databases

BioMutaiAOAH.
DMDMi113976.

Proteomic databases

EPDiP28039.
PaxDbiP28039.
PeptideAtlasiP28039.
PRIDEiP28039.

Protocols and materials databases

DNASUi313.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000612871; ENSP00000484305; ENSG00000136250. [P28039-2]
ENST00000617537; ENSP00000483783; ENSG00000136250. [P28039-1]
GeneIDi313.
KEGGihsa:313.
UCSCiuc032zjw.2. human. [P28039-1]

Organism-specific databases

CTDi313.
DisGeNETi313.
GeneCardsiAOAH.
HGNCiHGNC:548. AOAH.
HPAiHPA021666.
HPA026714.
HPA026716.
MIMi102593. gene.
neXtProtiNX_P28039.
OpenTargetsiENSG00000136250.
PharmGKBiPA24838.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IFCC. Eukaryota.
ENOG410XTCS. LUCA.
GeneTreeiENSGT00390000008427.
HOGENOMiHOG000008100.
HOVERGENiHBG004254.
InParanoidiP28039.
KOiK01065.
PhylomeDBiP28039.
TreeFamiTF329246.

Enzyme and pathway databases

BioCyciZFISH:HS06138-MONOMER.
BRENDAi3.1.1.77. 2681.

Miscellaneous databases

ChiTaRSiAOAH. human.
GeneWikiiAOAH.
GenomeRNAii313.
PROiP28039.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000136250.
CleanExiHS_AOAH.
ExpressionAtlasiP28039. baseline and differential.
GenevisibleiP28039. HS.

Family and domain databases

Gene3Di1.10.225.10. 1 hit.
3.40.50.1110. 2 hits.
InterProiIPR001087. GDSL.
IPR008138. SapB_2.
IPR011001. Saposin-like.
IPR008139. SaposinB_dom.
IPR013830. SGNH_hydro.
[Graphical view]
PfamiPF00657. Lipase_GDSL. 1 hit.
PF03489. SapB_2. 1 hit.
[Graphical view]
SMARTiSM00741. SapB. 1 hit.
[Graphical view]
SUPFAMiSSF47862. SSF47862. 1 hit.
SSF52266. SSF52266. 2 hits.
PROSITEiPS50015. SAP_B. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAOAH_HUMAN
AccessioniPrimary (citable) accession number: P28039
Secondary accession number(s): A4D1Y5, B7Z490, Q53F13
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: November 2, 2016
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.