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Protein

Acyloxyacyl hydrolase

Gene

AOAH

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Removes the secondary (acyloxyacyl-linked) fatty acyl chains from the lipid A region of bacterial lipopolysaccharides (PubMed:1883828, PubMed:8089145). By breaking down LPS, terminates the host response to bacterial infection and prevents prolonged and damaging inflammatory responses (By similarity). In peritoneal macrophages, seems to be important for recovery from a state of immune tolerance following infection by Gram-negative bacteria (By similarity).By similarity2 Publications

Catalytic activityi

3-(acyloxy)acyl group of bacterial toxin = 3-hydroxyacyl group of bacterial toxin + a fatty acid.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2631 Publication1

GO - Molecular functioni

  • acyloxyacyl hydrolase activity Source: UniProtKB-EC
  • catalytic activity Source: ProtInc
  • lipoprotein lipase activity Source: ProtInc

GO - Biological processi

  • inflammatory response Source: ProtInc
  • lipid metabolic process Source: ProtInc

Keywordsi

Molecular functionHydrolase

Enzyme and pathway databases

BRENDAi3.1.1.77. 2681.

Names & Taxonomyi

Protein namesi
Recommended name:
Acyloxyacyl hydrolase1 Publication (EC:3.1.1.772 Publications)
Cleaved into the following 2 chains:
Acyloxyacyl hydrolase small subunit1 Publication
Acyloxyacyl hydrolase large subunit1 Publication
Gene namesi
Name:AOAH1 Publication
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

EuPathDBiHostDB:ENSG00000136250.11.
HGNCiHGNC:548. AOAH.
MIMi102593. gene.
neXtProtiNX_P28039.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasmic vesicle, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi61T → A: Loss of glycosylation. No effect on enzyme activity or localization to cytoplasmic vesicles. 1 Publication1
Mutagenesisi263S → L: Significantly impairs catalytic activity. 1 Publication1

Organism-specific databases

DisGeNETi313.
OpenTargetsiENSG00000136250.
PharmGKBiPA24838.

Chemistry databases

GuidetoPHARMACOLOGYi2873.

Polymorphism and mutation databases

BioMutaiAOAH.
DMDMi113976.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 231 PublicationAdd BLAST23
PropeptideiPRO_000002073924 – 341 PublicationAdd BLAST11
ChainiPRO_000002074035 – 156Acyloxyacyl hydrolase small subunit1 PublicationAdd BLAST122
ChainiPRO_0000020741157 – 575Acyloxyacyl hydrolase large subunit1 PublicationAdd BLAST419

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi41 ↔ 114PROSITE-ProRule annotation
Disulfide bondi44 ↔ 108PROSITE-ProRule annotation
Glycosylationi59N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi70 ↔ 83PROSITE-ProRule annotation
Glycosylationi466N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

The small subunit is N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

EPDiP28039.
PaxDbiP28039.
PeptideAtlasiP28039.
PRIDEiP28039.

PTM databases

iPTMnetiP28039.
PhosphoSitePlusiP28039.

Expressioni

Gene expression databases

BgeeiENSG00000136250.
CleanExiHS_AOAH.
ExpressionAtlasiP28039. baseline and differential.
GenevisibleiP28039. HS.

Organism-specific databases

HPAiHPA021666.
HPA026716.

Interactioni

Subunit structurei

Heterodimer of the large and small subunits; disulfide-linked.2 Publications

Protein-protein interaction databases

IntActiP28039. 2 interactors.
STRINGi9606.ENSP00000258749.

Structurei

Secondary structure

1575
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi38 – 57Combined sources20
Helixi62 – 72Combined sources11
Helixi76 – 78Combined sources3
Helixi79 – 99Combined sources21
Helixi104 – 110Combined sources7
Beta strandi118 – 120Combined sources3
Helixi130 – 143Combined sources14
Helixi159 – 161Combined sources3
Helixi165 – 178Combined sources14
Beta strandi192 – 198Combined sources7
Turni199 – 201Combined sources3
Helixi221 – 223Combined sources3
Beta strandi224 – 226Combined sources3
Beta strandi230 – 232Combined sources3
Turni238 – 240Combined sources3
Helixi244 – 249Combined sources6
Beta strandi256 – 261Combined sources6
Turni263 – 267Combined sources5
Helixi272 – 274Combined sources3
Helixi277 – 279Combined sources3
Helixi282 – 285Combined sources4
Helixi288 – 293Combined sources6
Turni294 – 296Combined sources3
Helixi299 – 301Combined sources3
Turni303 – 305Combined sources3
Beta strandi311 – 313Combined sources3
Helixi319 – 326Combined sources8
Helixi328 – 330Combined sources3
Beta strandi334 – 338Combined sources5
Turni344 – 346Combined sources3
Helixi347 – 349Combined sources3
Helixi351 – 353Combined sources3
Turni358 – 360Combined sources3
Beta strandi364 – 369Combined sources6
Turni373 – 375Combined sources3
Beta strandi379 – 381Combined sources3
Turni383 – 385Combined sources3
Helixi389 – 404Combined sources16
Beta strandi411 – 416Combined sources6
Helixi423 – 428Combined sources6
Helixi434 – 436Combined sources3
Turni437 – 440Combined sources4
Helixi444 – 454Combined sources11
Turni460 – 462Combined sources3
Beta strandi463 – 465Combined sources3
Helixi467 – 490Combined sources24
Beta strandi496 – 501Combined sources6
Helixi505 – 514Combined sources10
Helixi519 – 522Combined sources4
Turni525 – 527Combined sources3
Beta strandi528 – 531Combined sources4
Helixi533 – 550Combined sources18
Helixi552 – 555Combined sources4
Helixi562 – 569Combined sources8
Turni570 – 573Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5W78X-ray2.27A24-152[»]
B153-575[»]
5W7CX-ray2.23A/B24-152[»]
C/D153-575[»]
ProteinModelPortaliP28039.
SMRiP28039.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini37 – 118Saposin B-typePROSITE-ProRule annotationAdd BLAST82

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni38 – 70Important for enzyme activity, localization to cytoplasmic vesicles, and protein stability1 PublicationAdd BLAST33

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IFCC. Eukaryota.
ENOG410XTCS. LUCA.
GeneTreeiENSGT00390000008427.
HOGENOMiHOG000008100.
HOVERGENiHBG004254.
InParanoidiP28039.
KOiK01065.
PhylomeDBiP28039.
TreeFamiTF329246.

Family and domain databases

InterProiView protein in InterPro
IPR001087. GDSL.
IPR008138. SapB_2.
IPR011001. Saposin-like.
IPR008139. SaposinB_dom.
PfamiView protein in Pfam
PF00657. Lipase_GDSL. 1 hit.
PF03489. SapB_2. 1 hit.
SMARTiView protein in SMART
SM00741. SapB. 1 hit.
SUPFAMiSSF47862. SSF47862. 1 hit.
PROSITEiView protein in PROSITE
PS50015. SAP_B. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P28039-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQSPWKILTV APLFLLLSLQ SSASPANDDQ SRPSLSNGHT CVGCVLVVSV
60 70 80 90 100
IEQLAQVHNS TVQASMERLC SYLPEKLFLK TTCYLVIDKF GSDIIKLLSA
110 120 130 140 150
DMNADVVCHT LEFCKQNTGQ PLCHLYPLPK ETWKFTLQKA RQIVKKSPIL
160 170 180 190 200
KYSRSGSDIC SLPVLAKICQ KIKLAMEQSV PFKDVDSDKY SVFPTLRGYH
210 220 230 240 250
WRGRDCNDSD ESVYPGRRPN NWDVHQDSNC NGIWGVDPKD GVPYEKKFCE
260 270 280 290 300
GSQPRGIILL GDSAGAHFHI SPEWITASQM SLNSFINLPT ALTNELDWPQ
310 320 330 340 350
LSGATGFLDS TVGIKEKSIY LRLWKRNHCN HRDYQNISRN GASSRNLKKF
360 370 380 390 400
IESLSRNKVL DYPAIVIYAM IGNDVCSGKS DPVPAMTTPE KLYSNVMQTL
410 420 430 440 450
KHLNSHLPNG SHVILYGLPD GTFLWDNLHN RYHPLGQLNK DMTYAQLYSF
460 470 480 490 500
LNCLQVSPCH GWMSSNKTLR TLTSERAEQL SNTLKKIAAS EKFTNFNLFY
510 520 530 540 550
MDFAFHEIIQ EWQKRGGQPW QLIEPVDGFH PNEVALLLLA DHFWKKVQLQ
560 570
WPQILGKENP FNPQIKQVFG DQGGH
Length:575
Mass (Da):65,105
Last modified:August 1, 1992 - v1
Checksum:iE3B3853DBD308AF7
GO
Isoform 2 (identifier: P28039-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     43-74: Missing.

Note: No experimental confirmation available.
Show »
Length:543
Mass (Da):61,647
Checksum:iBE14624794189A57
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti222W → C in BAD97196 (Ref. 6) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05066328D → N2 PublicationsCorresponds to variant dbSNP:rs2228410Ensembl.1
Natural variantiVAR_020133166A → T. Corresponds to variant dbSNP:rs3735384Ensembl.1
Natural variantiVAR_033513266A → G. Corresponds to variant dbSNP:rs3735386Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04257143 – 74Missing in isoform 2. 1 PublicationAdd BLAST32

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62840 mRNA. Translation: AAA35506.1.
AK297016 mRNA. Translation: BAH12476.1.
AK223476 mRNA. Translation: BAD97196.1.
AC083876 Genomic DNA. No translation available.
AC087069 Genomic DNA. No translation available.
CH236951 Genomic DNA. Translation: EAL23977.1.
CH471073 Genomic DNA. Translation: EAW94073.1.
BC025698 mRNA. Translation: AAH25698.1.
CCDSiCCDS5448.1. [P28039-1]
CCDS55102.1. [P28039-2]
PIRiA40292.
RefSeqiNP_001170977.1. NM_001177506.1.
NP_001170978.1. NM_001177507.1. [P28039-2]
NP_001628.1. NM_001637.3. [P28039-1]
UniGeneiHs.488007.
Hs.689297.

Genome annotation databases

EnsembliENST00000612871; ENSP00000484305; ENSG00000136250. [P28039-2]
ENST00000617537; ENSP00000483783; ENSG00000136250. [P28039-1]
GeneIDi313.
KEGGihsa:313.
UCSCiuc032zjw.2. human. [P28039-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiAOAH_HUMAN
AccessioniPrimary (citable) accession number: P28039
Secondary accession number(s): A4D1Y5, B7Z490, Q53F13
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: March 28, 2018
This is version 154 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome