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P28039

- AOAH_HUMAN

UniProt

P28039 - AOAH_HUMAN

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Protein
Acyloxyacyl hydrolase
Gene
AOAH
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Removes the secondary (acyloxyacyl-linked) fatty acyl chains from the lipid A region of bacterial lipopolysaccharides.

Catalytic activityi

3-(acyloxy)acyl group of bacterial toxin = 3-hydroxyacyl group of bacterial toxin + a fatty acid.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei263 – 2631 Reviewed prediction

GO - Molecular functioni

  1. acyloxyacyl hydrolase activity Source: UniProtKB-EC
  2. catalytic activity Source: ProtInc
  3. lipoprotein lipase activity Source: ProtInc

GO - Biological processi

  1. inflammatory response Source: ProtInc
  2. lipid metabolic process Source: ProtInc
  3. lipopolysaccharide metabolic process Source: Ensembl
  4. negative regulation of inflammatory response Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BRENDAi3.1.1.77. 2681.

Names & Taxonomyi

Protein namesi
Recommended name:
Acyloxyacyl hydrolase (EC:3.1.1.77)
Cleaved into the following 2 chains:
Gene namesi
Name:AOAH
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 7

Organism-specific databases

HGNCiHGNC:548. AOAH.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA24838.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323 Reviewed prediction
Add
BLAST
Propeptidei24 – 3411
PRO_0000020739Add
BLAST
Chaini35 – 156122Acyloxyacyl hydrolase small subunit
PRO_0000020740Add
BLAST
Chaini157 – 575419Acyloxyacyl hydrolase large subunit
PRO_0000020741Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi41 ↔ 114 By similarity
Disulfide bondi44 ↔ 108 By similarity
Glycosylationi59 – 591N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi70 ↔ 83 By similarity
Disulfide bondi123 ↔ ?
Glycosylationi207 – 2071N-linked (GlcNAc...) Reviewed prediction
Glycosylationi336 – 3361N-linked (GlcNAc...) Reviewed prediction
Glycosylationi409 – 4091N-linked (GlcNAc...) Reviewed prediction
Glycosylationi466 – 4661N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

Both subunits contain a number of cysteine residues that may form disulfide bridges.

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP28039.
PRIDEiP28039.

PTM databases

PhosphoSiteiP28039.

Expressioni

Gene expression databases

ArrayExpressiP28039.
BgeeiP28039.
CleanExiHS_AOAH.
GenevestigatoriP28039.

Organism-specific databases

HPAiHPA021666.
HPA026714.
HPA026716.

Interactioni

Subunit structurei

Heterodimer.

Protein-protein interaction databases

IntActiP28039. 1 interaction.
STRINGi9606.ENSP00000258749.

Structurei

3D structure databases

ProteinModelPortaliP28039.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini37 – 11882Saposin B-type
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG47076.
HOGENOMiHOG000008100.
HOVERGENiHBG004254.
InParanoidiP28039.
KOiK01065.
OrthoDBiEOG75MVVQ.
PhylomeDBiP28039.
TreeFamiTF329246.

Family and domain databases

Gene3Di1.10.225.10. 1 hit.
3.40.50.1110. 2 hits.
InterProiIPR001087. Lipase_GDSL.
IPR008138. SapB_2.
IPR011001. Saposin-like.
IPR008139. SaposinB.
IPR013831. SGNH_hydro-type_esterase_dom.
[Graphical view]
PfamiPF00657. Lipase_GDSL. 1 hit.
PF03489. SapB_2. 1 hit.
[Graphical view]
SMARTiSM00741. SapB. 1 hit.
[Graphical view]
SUPFAMiSSF47862. SSF47862. 1 hit.
PROSITEiPS50015. SAP_B. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P28039-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MQSPWKILTV APLFLLLSLQ SSASPANDDQ SRPSLSNGHT CVGCVLVVSV    50
IEQLAQVHNS TVQASMERLC SYLPEKLFLK TTCYLVIDKF GSDIIKLLSA 100
DMNADVVCHT LEFCKQNTGQ PLCHLYPLPK ETWKFTLQKA RQIVKKSPIL 150
KYSRSGSDIC SLPVLAKICQ KIKLAMEQSV PFKDVDSDKY SVFPTLRGYH 200
WRGRDCNDSD ESVYPGRRPN NWDVHQDSNC NGIWGVDPKD GVPYEKKFCE 250
GSQPRGIILL GDSAGAHFHI SPEWITASQM SLNSFINLPT ALTNELDWPQ 300
LSGATGFLDS TVGIKEKSIY LRLWKRNHCN HRDYQNISRN GASSRNLKKF 350
IESLSRNKVL DYPAIVIYAM IGNDVCSGKS DPVPAMTTPE KLYSNVMQTL 400
KHLNSHLPNG SHVILYGLPD GTFLWDNLHN RYHPLGQLNK DMTYAQLYSF 450
LNCLQVSPCH GWMSSNKTLR TLTSERAEQL SNTLKKIAAS EKFTNFNLFY 500
MDFAFHEIIQ EWQKRGGQPW QLIEPVDGFH PNEVALLLLA DHFWKKVQLQ 550
WPQILGKENP FNPQIKQVFG DQGGH 575
Length:575
Mass (Da):65,105
Last modified:August 1, 1992 - v1
Checksum:iE3B3853DBD308AF7
GO
Isoform 2 (identifier: P28039-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     43-74: Missing.

Note: No experimental confirmation available.

Show »
Length:543
Mass (Da):61,647
Checksum:iBE14624794189A57
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti28 – 281D → N.2 Publications
Corresponds to variant rs11976480 [ dbSNP | Ensembl ].
VAR_050663
Natural varianti166 – 1661A → T.
Corresponds to variant rs3735384 [ dbSNP | Ensembl ].
VAR_020133
Natural varianti266 – 2661A → G.
Corresponds to variant rs3735386 [ dbSNP | Ensembl ].
VAR_033513

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei43 – 7432Missing in isoform 2.
VSP_042571Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti222 – 2221W → C in BAD97196. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M62840 mRNA. Translation: AAA35506.1.
AK297016 mRNA. Translation: BAH12476.1.
AK223476 mRNA. Translation: BAD97196.1.
AC083876 Genomic DNA. No translation available.
AC087069 Genomic DNA. No translation available.
CH236951 Genomic DNA. Translation: EAL23977.1.
CH471073 Genomic DNA. Translation: EAW94073.1.
BC025698 mRNA. Translation: AAH25698.1.
CCDSiCCDS5448.1. [P28039-1]
CCDS55102.1. [P28039-2]
PIRiA40292.
RefSeqiNP_001170977.1. NM_001177506.1.
NP_001170978.1. NM_001177507.1. [P28039-2]
NP_001628.1. NM_001637.3. [P28039-1]
UniGeneiHs.488007.

Genome annotation databases

EnsembliENST00000258749; ENSP00000258749; ENSG00000136250. [P28039-1]
ENST00000535891; ENSP00000441101; ENSG00000136250. [P28039-2]
GeneIDi313.
KEGGihsa:313.
UCSCiuc003tfh.4. human. [P28039-1]
uc011kba.2. human. [P28039-2]

Polymorphism databases

DMDMi113976.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M62840 mRNA. Translation: AAA35506.1 .
AK297016 mRNA. Translation: BAH12476.1 .
AK223476 mRNA. Translation: BAD97196.1 .
AC083876 Genomic DNA. No translation available.
AC087069 Genomic DNA. No translation available.
CH236951 Genomic DNA. Translation: EAL23977.1 .
CH471073 Genomic DNA. Translation: EAW94073.1 .
BC025698 mRNA. Translation: AAH25698.1 .
CCDSi CCDS5448.1. [P28039-1 ]
CCDS55102.1. [P28039-2 ]
PIRi A40292.
RefSeqi NP_001170977.1. NM_001177506.1.
NP_001170978.1. NM_001177507.1. [P28039-2 ]
NP_001628.1. NM_001637.3. [P28039-1 ]
UniGenei Hs.488007.

3D structure databases

ProteinModelPortali P28039.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P28039. 1 interaction.
STRINGi 9606.ENSP00000258749.

PTM databases

PhosphoSitei P28039.

Polymorphism databases

DMDMi 113976.

Proteomic databases

PaxDbi P28039.
PRIDEi P28039.

Protocols and materials databases

DNASUi 313.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000258749 ; ENSP00000258749 ; ENSG00000136250 . [P28039-1 ]
ENST00000535891 ; ENSP00000441101 ; ENSG00000136250 . [P28039-2 ]
GeneIDi 313.
KEGGi hsa:313.
UCSCi uc003tfh.4. human. [P28039-1 ]
uc011kba.2. human. [P28039-2 ]

Organism-specific databases

CTDi 313.
GeneCardsi GC07M036519.
HGNCi HGNC:548. AOAH.
HPAi HPA021666.
HPA026714.
HPA026716.
MIMi 102593. gene.
neXtProti NX_P28039.
PharmGKBi PA24838.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG47076.
HOGENOMi HOG000008100.
HOVERGENi HBG004254.
InParanoidi P28039.
KOi K01065.
OrthoDBi EOG75MVVQ.
PhylomeDBi P28039.
TreeFami TF329246.

Enzyme and pathway databases

BRENDAi 3.1.1.77. 2681.

Miscellaneous databases

ChiTaRSi AOAH. human.
GeneWikii AOAH.
GenomeRNAii 313.
NextBioi 1273.
PROi P28039.
SOURCEi Search...

Gene expression databases

ArrayExpressi P28039.
Bgeei P28039.
CleanExi HS_AOAH.
Genevestigatori P28039.

Family and domain databases

Gene3Di 1.10.225.10. 1 hit.
3.40.50.1110. 2 hits.
InterProi IPR001087. Lipase_GDSL.
IPR008138. SapB_2.
IPR011001. Saposin-like.
IPR008139. SaposinB.
IPR013831. SGNH_hydro-type_esterase_dom.
[Graphical view ]
Pfami PF00657. Lipase_GDSL. 1 hit.
PF03489. SapB_2. 1 hit.
[Graphical view ]
SMARTi SM00741. SapB. 1 hit.
[Graphical view ]
SUPFAMi SSF47862. SSF47862. 1 hit.
PROSITEi PS50015. SAP_B. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Expression and characterization of recombinant human acyloxyacyl hydrolase, a leukocyte enzyme that deacylates bacterial lipopolysaccharides."
    Hagen F.S., Grant F.J., Kuijper J.L., Slaughter C.A., Moomaw C.R., Orth K., O'Hara P.J., Munford R.S.
    Biochemistry 30:8415-8423(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 35-49 AND 157-185.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Umbilical cord blood.
  3. Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
    Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Synovial cell.
  4. "The DNA sequence of human chromosome 7."
    Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L.
    , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
    Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "Human chromosome 7: DNA sequence and biology."
    Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S.
    , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C.
    Science 300:767-772(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ASN-28.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ASN-28.
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Pancreas.

Entry informationi

Entry nameiAOAH_HUMAN
AccessioniPrimary (citable) accession number: P28039
Secondary accession number(s): A4D1Y5, B7Z490, Q53F13
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: July 9, 2014
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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