Reviewed, UniProtKB/Swiss-Prot P28038 (FL3H_HORVU)

Names and origin

Protein names	Recommended name:     Naringenin,2-oxoglutarate 3-dioxygenase     EC=1.14.11.9 Alternative name(s):     Flavonone-3-hydroxylase       Short name=F3H     FHT
Organism	Hordeum vulgare (Barley)
Taxonomic identifier	4513 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Hordeum

Protein attributes

Sequence length	377 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the 3-beta-hydroxylation of 2S-flavonones to 2R,3R-dihydroflavonols which are intermediates in the biosynthesis of flavonols, anthocyanidins, catechins and proanthocyanidins in plants.
Catalytic activity	A flavanone + 2-oxoglutarate + O2 = a dihydroflavonol + succinate + CO2.
Cofactor	Iron. Ascorbate.
Pathway	Secondary metabolite biosynthesis; flavonoid biosynthesis.
Sequence similarities	Belongs to the iron/ascorbate-dependent oxidoreductase family.

Ontologies

Keywords
Biological process	Flavonoid biosynthesis
Ligand	Iron Metal-binding Vitamin C
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	flavonoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-ascorbic acid binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW naringenin 3-dioxygenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 377	377	Naringenin,2-oxoglutarate 3-dioxygenase		PRO_0000067286
Sites
	Metal binding	78	1	Iron Potential
	Metal binding	220	1	Iron Potential
	Metal binding	222	1	Iron Potential
	Metal binding	278	1	Iron Potential

Sequences

	Sequence		Length	Mass (Da)
	P28038-1 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 13813A85BB826EF0	FASTA	377	42,057
	10 20 30 40 50 60 MAPVSNETFL PTEAWGEATL RPSFVRDEDE RPKVAHDRFS DAVPLISLHG IDGARRAQIR 70 80 90 100 110 120 DRVAAACEDW GIFQVIDHGV DADLIADMTR LAREFFALPA EDKLRYDMSG GKKGGFIVSS 130 140 150 160 170 180 HLQGEAVQDW REIVTYFSYP VKARDYGRWP EKPAGWCAVV ERYSERLMGL SCNLMGVLSE 190 200 210 220 230 240 AMGLETEALA KACVDMDQKV VVNFYPRCPQ PDLTLGLKRH TDPGTITLLL QDLVGGLQAT 250 260 270 280 290 300 RDGGKNWITV QPISGAFVVN LGDHGHFMSN GRFKNADHQA VVNGESSRLS IATFQNPAPD 310 320 330 340 350 360 ARVWPLAVRE GEEPILEEPI TFTEMYRRKM ERDLDLAKRK KQAKDQLMQQ QLQLQQQQAV 370 AAAPMPTATK PLNEILA

References

[1]

"Expression of chalcone synthase, dihydroflavonol reductase and flavanone-3-hydroxylase in mutants of barley deficient in anthocyanin and proantocyanidin biosynthesis." Meldgaard M. Theor. Appl. Genet. 83:695-706(1992) [Agricola: IND92043518] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Triumph. Tissue: Testa.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X58138 mRNA. Translation: CAA41146.1.
PIR	S14647.
3D structure databases
HSSP	HSSP built from PDB template 1GP6 based on UniProtKB Q96323.
ModBase	Search...
Organism-specific databases
Gramene	P28038.
Enzyme and pathway databases
BRENDA	1.14.11.9. 283.
Family and domain databases
InterPro	IPR005123. Oxoglutarate/Fe-dep_Oase. [Graphical view]
Pfam	PF03171. 2OG-FeII_Oxy. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FL3H_HORVU

Accession

Primary (citable) accession number: P28038

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	May 5, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)