P28033 (CEBPB_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CCAAT/enhancer-binding protein beta
Short name=C/EBP beta

Alternative name(s):
AGP/EBP
Interleukin-6-dependent-binding protein
Short name=IL-6DBP
Liver-enriched transcriptional activator
Short name=LAP

Gene names

Name:

Cebpb

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	296 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in interleukin-6 signal transduction, including the transcriptional activation of acute-phase genes. Functions in brown adipose tissue (BAT) differentiation. Regulates the transcriptional induction of peroxisome proliferator-activated receptor gamma (PPARG). Ref.8
Subunit structure	Binds DNA as a dimer and can form stable heterodimers with C/EBP alpha and gamma. Interacts with TRIM28 and PTGES2. Interacts with PRDM16. Interacts with CCDC85B. Forms a complex with THOC5. Interacts with ZNF638; this interaction increases transcriptional activation. Interacts with CIDEA and CIDEC. Interaction with CIDEA increases transcriptional activation of a subset of CEBPB downstream target genes, including ID2, IGF1, PRLR, SOCS1, SOCS3, XDH. Interaction with CIDEC increases transcriptional activation of SOCS1, SOCS3, TGFB1, TGFBR1, ID2 and XDH. Interacts with DDIT3/CHOP By similarity. Ref.4 Ref.5 Ref.6 Ref.8 Ref.9 Ref.10
Subcellular location	Nucleus Ref.7.
Tissue specificity	Abundantly expressed in myoblasts. Enriched in brown adipose tissue (BAT) versus white adipose tissue (WAT). Ref.8
Induction	Up-regulated by cold exposure. Ref.8
Post-translational modification	Sumoylated by polymeric chains of SUMO2 or SUMO3 By similarity. Phosphorylated at Thr-188 by RPS6KA1 By similarity.
Disruption phenotype	Mice die in 24 hours after birth. Embryos display defects in brown fat tissue development. Ref.8
Sequence similarities	Belongs to the bZIP family. C/EBP subfamily. Contains 1 bZIP (basic-leucine zipper) domain.

Ontologies

Keywords
Biological process	Differentiation Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Activator
PTM	Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	brown fat cell differentiation Inferred from mutant phenotype Ref.8. Source: UniProtKB cellular response to amino acid stimulus Inferred from direct assay PubMed 20548288. Source: MGI embryonic placenta development Inferred from genetic interaction PubMed 15509779. Source: MGI induction of apoptosis Inferred from direct assay PubMed 11733516. Source: MGI mammary gland epithelial cell differentiation Inferred from mutant phenotype PubMed 10707954. Source: MGI mammary gland epithelial cell proliferation Inferred from mutant phenotype PubMed 10683373. Source: MGI negative regulation of apoptotic process Inferred from direct assay PubMed 11684016. Source: MGI negative regulation of transcription, DNA-dependent Inferred from direct assay PubMed 15175325. Source: MGI neuron differentiation Inferred from direct assay PubMed 11733516. Source: MGI positive regulation of osteoblast differentiation Inferred from direct assay PubMed 15175325. Source: MGI regulation of interleukin-6 biosynthetic process Inferred from direct assay PubMed 11976687. Source: MGI response to endoplasmic reticulum stress Inferred from sequence or structural similarity. Source: UniProtKB response to lipopolysaccharide Inferred from mutant phenotype PubMed 17724128. Source: BHF-UCL
Cellular_component	cytoplasm Inferred from direct assay PubMed 11684016PubMed 15870285. Source: MGI nuclear chromatin Inferred from direct assay PubMed 15187136. Source: BHF-UCL nuclear matrix Inferred from electronic annotation. Source: Compara nucleoplasm Traceable author statement. Source: Reactome
Molecular_function	RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Inferred from direct assay PubMed 10588870. Source: MGI RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Inferred from direct assay Ref.8. Source: UniProtKB protein heterodimerization activity Inferred from direct assay PubMed 12177065. Source: UniProtKB protein homodimerization activity Inferred from direct assay PubMed 12177065. Source: UniProtKB sequence-specific DNA binding Inferred from direct assay PubMed 10588870PubMed 15659391. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 296

296

CCAAT/enhancer-binding protein beta

PRO_0000076618

Regions

Domain

222 – 285

64

bZIP

Region

1 – 22

22

Required for Lys-174 sumoylation By similarity

Region

226 – 246

21

Basic motif By similarity

Region

248 – 255

8

Leucine-zipper By similarity

Compositional bias

120 – 129

10

Pro-rich

Compositional bias

170 – 191

22

Pro/Ser-rich

Amino acid modifications

Modified residue

188

1

Phosphothreonine; by RPS6KA1 By similarity

Cross-link

133

Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity

Secondary structure

1

.

.

.

296

Helix Strand Turn

Details...

Helix

240 – 282

43

Sequences

	Sequence		Length	Mass (Da)
	P28033 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 827AC4AFC209AE89	FASTA	296	31,446
	10 20 30 40 50 60 MHRLLAWDAA CLPPPPAAFR PMEVANFYYE PDCLAYGAKA ARAAPRAPAA EPAIGEHERA 70 80 90 100 110 120 IDFSPYLEPL APAADFAAPA PAHHDFLSDL FADDYGAKPS KKPADYGYVS LGRAGAKAAP 130 140 150 160 170 180 PACFPPPPPA ALKAEPGFEP ADCKRADDAP AMAAGFPFAL RAYLGYQATP SGSSGSLSTS 190 200 210 220 230 240 SSSSPPGTPS PADAKAAPAA CFAGPPAAPA KAKAKKTVDK LSDEYKMRRE RNNIAVRKSR 250 260 270 280 290 DKAKMRNLET QHKVLELTAE NERLQKKVEQ LSRELSTLRN LFKQLPEPLL ASAGHC

References

[1]	"Molecular cloning of a transcription factor, AGP/EBP, that belongs to members of the C/EBP family." Chang C.J., Chen T.T., Lei H.Y., Chen D.S., Lee S.C. Mol. Cell. Biol. 10:6642-6653(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Liver.
[2]	"Regulated expression of three C/EBP isoforms during adipose conversion of 3T3-L1 cells." Cao Z., Umek R.M., McKnight S.L. Genes Dev. 5:1538-1552(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Autoregulated induction of the acute-phase response transcription factor gene, agp/ebp." Chang C.J., Shen B.J., Lee S.C. DNA Cell Biol. 14:529-537(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11. Tissue: Liver.
[4]	"Coactivator TIF1beta interacts with transcription factor C/EBPbeta and glucocorticoid receptor to induce alpha1-acid glycoprotein gene expression." Chang C.J., Chen Y.L., Lee S.C. Mol. Cell. Biol. 18:5880-5887(1998) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH TRIM28.
[5]	"Delta-interacting protein A, a new inhibitory partner of CCAAT/enhancer-binding protein beta, implicated in adipocyte differentiation." Bezy O., Elabd C., Cochet O., Petersen R.K., Kristiansen K., Dani C., Ailhaud G., Amri E.-Z. J. Biol. Chem. 280:11432-11438(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CCDC85B.
[6]	"IFN-gamma-stimulated transcriptional activation by IFN-gamma-activated transcriptional element-binding factor 1 occurs via an inducible interaction with CAAAT/enhancer-binding protein-beta." Meng Q., Raha A., Roy S., Hu J., Kalvakolanu D.V. J. Immunol. 174:6203-6211(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PTGES2.
[7]	"THOC5 couples M-CSF receptor signaling to transcription factor expression." Carney L., Pierce A., Rijnen M., Gonzalez Sanchez M.B., Hamzah H.G., Zhang L., Tamura T., Whetton A.D. Cell. Signal. 21:309-316(2009) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, COMPLEX FORMATION WITH THOC5.
[8]	"Initiation of myoblast to brown fat switch by a PRDM16-C/EBP-beta transcriptional complex." Kajimura S., Seale P., Kubota K., Lunsford E., Frangioni J.V., Gygi S.P., Spiegelman B.M. Nature 460:1154-1158(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH PRDM16, INDUCTION BY COLD EXPOSURE, TISSUE SPECIFICITY.
[9]	"Regulation of adipocyte differentiation by the zinc finger protein ZNF638." Meruvu S., Hugendubler L., Mueller E. J. Biol. Chem. 286:26516-26523(2011) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ZNF638.
[10]	"Cidea is an essential transcriptional coactivator regulating mammary gland secretion of milk lipids." Wang W., Lv N., Zhang S., Shui G., Qian H., Zhang J., Chen Y., Ye J., Xie Y., Shen Y., Wenk M.R., Li P. Nat. Med. 18:235-243(2012) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CIDEA AND CIDEC.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M61007 mRNA. Translation: AAA37192.1.
X62600 mRNA. Translation: CAA44484.1.
S78572 Genomic DNA. No translation available.

IPI

IPI00116613.

PIR

A36366.

RefSeq

NP_034013.1. NM_009883.3.

UniGene

Mm.439656.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1CI6	X-ray	2.60	B	224-285	[»]

ProteinModelPortal

P28033.

SMR

P28033. Positions 239-285.

ModBase

Search...

Protein-protein interaction databases

IntAct

P28033. 1 interaction.

STRING

10090.ENSMUSP00000069850.

PTM databases

PhosphoSite

P28033.

Proteomic databases

PRIDE

P28033.

Protocols and materials databases

DNASU

12608.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000070642; ENSMUSP00000069850; ENSMUSG00000056501.

GeneID

12608.

KEGG

mmu:12608.

Organism-specific databases

CTD

1051.

MGI

MGI:88373. Cebpb.

Phylogenomic databases

eggNOG

NOG299311.

HOGENOM

HOG000013112.

HOVERGEN

HBG050879.

InParanoid

P28033.

KO

K10048.

OMA

CKKPAEY.

OrthoDB

EOG4M65JR.

Enzyme and pathway databases

Reactome

REACT_127416. Developmental Biology.
REACT_27166. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Gene expression databases

ArrayExpress

P28033.

Bgee

P28033.

CleanEx

MM_CEBPB.

Genevestigator

P28033.

GermOnline

ENSMUSG00000056501. Mus musculus.

Family and domain databases

InterPro

IPR004827. bZIP.
IPR016468. CCAAT/enhancer-binding.
[Graphical view]

Pfam

PF07716. bZIP_2. 1 hit.
[Graphical view]

PIRSF

PIRSF005879. CCAAT/enhancer-binding. 1 hit.

SMART

SM00338. BRLZ. 1 hit.
[Graphical view]

PROSITE

PS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. False negative.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

CEBPB. mouse.

EvolutionaryTrace

P28033.

NextBio

281758.

SOURCE

Search...

Entry information

Entry name

CEBPB_MOUSE

Accession

Primary (citable) accession number: P28033

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	April 3, 2013
This is version 126 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program