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P28033

- CEBPB_MOUSE

UniProt

P28033 - CEBPB_MOUSE

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Protein
CCAAT/enhancer-binding protein beta
Gene
Cebpb
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Important transcription factor regulating the expression of genes involved in immune and inflammatory responses. Binds to regulatory regions of several acute-phase and cytokines genes and probably plays a role in the regulation of acute-phase reaction, inflammation and hemopoiesis. The consensus recognition site is 5'-T[TG]NNGNAA[TG]-3'. Plays an important role in adipose tissue differentiation. Regulates the transcriptional induction of peroxisome proliferator-activated receptor gamma (PPARG) as well as other adipogenesis key player genes.2 Publications
Isoform 3: acts as a dominant negative through heterodimerization with isoform 2 By similarity.2 Publications

GO - Molecular functioni

  1. DNA binding Source: MGI
  2. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: MGI
  3. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Source: UniProtKB
  4. chromatin binding Source: MGI
  5. protein binding Source: UniProtKB
  6. protein heterodimerization activity Source: UniProtKB
  7. protein homodimerization activity Source: UniProtKB
  8. sequence-specific DNA binding Source: MGI
Complete GO annotation...

GO - Biological processi

  1. brown fat cell differentiation Source: UniProtKB
  2. cellular response to amino acid stimulus Source: MGI
  3. embryonic placenta development Source: MGI
  4. fat cell differentiation Source: MGI
  5. mammary gland epithelial cell differentiation Source: MGI
  6. mammary gland epithelial cell proliferation Source: MGI
  7. negative regulation of neuron apoptotic process Source: MGI
  8. negative regulation of transcription, DNA-templated Source: MGI
  9. neuron differentiation Source: MGI
  10. positive regulation of fat cell differentiation Source: UniProtKB
  11. positive regulation of osteoblast differentiation Source: MGI
  12. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  13. positive regulation of transcription, DNA-templated Source: MGI
  14. regulation of interleukin-6 biosynthetic process Source: MGI
  15. regulation of transcription involved in cell fate commitment Source: UniProtKB
  16. regulation of transcription, DNA-templated Source: UniProtKB
  17. response to endoplasmic reticulum stress Source: UniProtKB
  18. response to lipopolysaccharide Source: BHF-UCL
  19. transcription from RNA polymerase II promoter Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Differentiation, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_206033. Senescence-Associated Secretory Phenotype (SASP).
REACT_27166. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Names & Taxonomyi

Protein namesi
Recommended name:
CCAAT/enhancer-binding protein beta
Short name:
C/EBP beta
Alternative name(s):
AGP/EBP
Interleukin-6-dependent-binding protein
Short name:
IL-6DBP
Liver-enriched transcriptional activator
Short name:
LAP
Gene namesi
Name:Cebpb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:88373. Cebpb.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. condensed chromosome, centromeric region Source: MGI
  2. cytoplasm Source: MGI
  3. nuclear chromatin Source: BHF-UCL
  4. nuclear matrix Source: MGI
  5. nucleoplasm Source: Reactome
  6. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice die in 24 hours after birth. Embryos display defects in brown fat tissue development.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 296296CCAAT/enhancer-binding protein beta
PRO_0000076618Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki133 – 133Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity
Modified residuei188 – 1881Phosphothreonine; by RPS6KA11 Publication

Post-translational modificationi

Sumoylated by polymeric chains of SUMO2 or SUMO3 By similarity.

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP28033.
PRIDEiP28033.

PTM databases

PhosphoSiteiP28033.

Expressioni

Tissue specificityi

Abundantly expressed in myoblasts. Enriched in brown adipose tissue (BAT) versus white adipose tissue (WAT).1 Publication

Inductioni

Up-regulated by cold exposure.1 Publication

Gene expression databases

ArrayExpressiP28033.
BgeeiP28033.
CleanExiMM_CEBPB.
GenevestigatoriP28033.

Interactioni

Subunit structurei

Binds DNA as a dimer and can form stable heterodimers with CEBPA, CEBPD and CEBPG. Interacts with TRIM28 and PTGES2. Interacts with PRDM16. Interacts with CCDC85B. Forms a complex with THOC5. Interacts with ZNF638; this interaction increases transcriptional activation. Interacts with CIDEA and CIDEC. Interaction with CIDEA increases transcriptional activation of a subset of CEBPB downstream target genes, including ID2, IGF1, PRLR, SOCS1, SOCS3, XDH. Interaction with CIDEC increases transcriptional activation of SOCS1, SOCS3, TGFB1, TGFBR1, ID2 and XDH. Interacts with DDIT3/CHOP. Interacts with EP300; recruits EP300 to chromatin. Interacts with RORA; the interaction disrupts interaction with EP300.7 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Bhlhe41Q99PV55EBI-1029979,EBI-6143801

Protein-protein interaction databases

BioGridi198669. 12 interactions.
DIPiDIP-37539N.
IntActiP28033. 7 interactions.
STRINGi10090.ENSMUSP00000069850.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi240 – 28243

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CI6X-ray2.60B224-285[»]
ProteinModelPortaliP28033.
SMRiP28033. Positions 239-285.

Miscellaneous databases

EvolutionaryTraceiP28033.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini222 – 28564bZIP
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 2222Required for Lys-174 sumoylation By similarity
Add
BLAST
Regioni226 – 24621Basic motif By similarity
Add
BLAST
Regioni248 – 2558Leucine-zipper By similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi120 – 12910Pro-rich
Compositional biasi170 – 19122Pro/Ser-rich
Add
BLAST

Sequence similaritiesi

Belongs to the bZIP family. C/EBP subfamily.

Phylogenomic databases

eggNOGiNOG299311.
HOGENOMiHOG000013112.
HOVERGENiHBG050879.
InParanoidiP28033.
KOiK10048.
OMAiCKKPAEY.
OrthoDBiEOG7R56TQ.
PhylomeDBiP28033.
TreeFamiTF105008.

Family and domain databases

InterProiIPR004827. bZIP.
IPR016468. CCAAT/enhancer-binding.
[Graphical view]
PfamiPF07716. bZIP_2. 1 hit.
[Graphical view]
PIRSFiPIRSF005879. CCAAT/enhancer-binding. 1 hit.
SMARTiSM00338. BRLZ. 1 hit.
[Graphical view]
PROSITEiPS50217. BZIP. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative initiation. Align

Isoform 1 (identifier: P28033-1) [UniParc]FASTAAdd to Basket

Also known as: a, C/EBPbeta-FL

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MHRLLAWDAA CLPPPPAAFR PMEVANFYYE PDCLAYGAKA ARAAPRAPAA    50
EPAIGEHERA IDFSPYLEPL APAADFAAPA PAHHDFLSDL FADDYGAKPS 100
KKPADYGYVS LGRAGAKAAP PACFPPPPPA ALKAEPGFEP ADCKRADDAP 150
AMAAGFPFAL RAYLGYQATP SGSSGSLSTS SSSSPPGTPS PADAKAAPAA 200
CFAGPPAAPA KAKAKKTVDK LSDEYKMRRE RNNIAVRKSR DKAKMRNLET 250
QHKVLELTAE NERLQKKVEQ LSRELSTLRN LFKQLPEPLL ASAGHC 296
Length:296
Mass (Da):31,446
Last modified:August 1, 1992 - v1
Checksum:i827AC4AFC209AE89
GO
Isoform 2 (identifier: P28033-3) [UniParc]FASTAAdd to Basket

Also known as: b, C/EBPbeta-LAP

The sequence of this isoform differs from the canonical sequence as follows:
     1-21: Missing.

Note: Major isoform.

Show »
Length:275
Mass (Da):29,133
Checksum:i717E36841E48752D
GO
Isoform 3 (identifier: P28033-2) [UniParc]FASTAAdd to Basket

Also known as: c, C/EBPbeta-LIP

The sequence of this isoform differs from the canonical sequence as follows:
     1-151: Missing.

Show »
Length:145
Mass (Da):15,597
Checksum:iC7AFEEE4F98F5978
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 151151Missing in isoform 3.
VSP_053976Add
BLAST
Alternative sequencei1 – 2121Missing in isoform 2.
VSP_053977Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M61007 mRNA. Translation: AAA37192.1.
X62600 mRNA. Translation: CAA44484.1.
S78572 Genomic DNA. No translation available.
CCDSiCCDS17105.1. [P28033-1]
PIRiA36366.
RefSeqiNP_001274667.1. NM_001287738.1. [P28033-3]
NP_001274668.1. NM_001287739.1. [P28033-2]
NP_034013.1. NM_009883.4. [P28033-1]
UniGeneiMm.439656.

Genome annotation databases

EnsembliENSMUST00000070642; ENSMUSP00000069850; ENSMUSG00000056501. [P28033-1]
GeneIDi12608.
KEGGimmu:12608.
UCSCiuc008oaf.1. mouse. [P28033-1]

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M61007 mRNA. Translation: AAA37192.1 .
X62600 mRNA. Translation: CAA44484.1 .
S78572 Genomic DNA. No translation available.
CCDSi CCDS17105.1. [P28033-1 ]
PIRi A36366.
RefSeqi NP_001274667.1. NM_001287738.1. [P28033-3 ]
NP_001274668.1. NM_001287739.1. [P28033-2 ]
NP_034013.1. NM_009883.4. [P28033-1 ]
UniGenei Mm.439656.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1CI6 X-ray 2.60 B 224-285 [» ]
ProteinModelPortali P28033.
SMRi P28033. Positions 239-285.
ModBasei Search...

Protein-protein interaction databases

BioGridi 198669. 12 interactions.
DIPi DIP-37539N.
IntActi P28033. 7 interactions.
STRINGi 10090.ENSMUSP00000069850.

PTM databases

PhosphoSitei P28033.

Proteomic databases

MaxQBi P28033.
PRIDEi P28033.

Protocols and materials databases

DNASUi 12608.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000070642 ; ENSMUSP00000069850 ; ENSMUSG00000056501 . [P28033-1 ]
GeneIDi 12608.
KEGGi mmu:12608.
UCSCi uc008oaf.1. mouse. [P28033-1 ]

Organism-specific databases

CTDi 1051.
MGIi MGI:88373. Cebpb.

Phylogenomic databases

eggNOGi NOG299311.
HOGENOMi HOG000013112.
HOVERGENi HBG050879.
InParanoidi P28033.
KOi K10048.
OMAi CKKPAEY.
OrthoDBi EOG7R56TQ.
PhylomeDBi P28033.
TreeFami TF105008.

Enzyme and pathway databases

Reactomei REACT_206033. Senescence-Associated Secretory Phenotype (SASP).
REACT_27166. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Miscellaneous databases

ChiTaRSi CEBPB. mouse.
EvolutionaryTracei P28033.
NextBioi 281758.
PROi P28033.
SOURCEi Search...

Gene expression databases

ArrayExpressi P28033.
Bgeei P28033.
CleanExi MM_CEBPB.
Genevestigatori P28033.

Family and domain databases

InterProi IPR004827. bZIP.
IPR016468. CCAAT/enhancer-binding.
[Graphical view ]
Pfami PF07716. bZIP_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF005879. CCAAT/enhancer-binding. 1 hit.
SMARTi SM00338. BRLZ. 1 hit.
[Graphical view ]
PROSITEi PS50217. BZIP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning of a transcription factor, AGP/EBP, that belongs to members of the C/EBP family."
    Chang C.J., Chen T.T., Lei H.Y., Chen D.S., Lee S.C.
    Mol. Cell. Biol. 10:6642-6653(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Liver.
  2. "Regulated expression of three C/EBP isoforms during adipose conversion of 3T3-L1 cells."
    Cao Z., Umek R.M., McKnight S.L.
    Genes Dev. 5:1538-1552(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Autoregulated induction of the acute-phase response transcription factor gene, agp/ebp."
    Chang C.J., Shen B.J., Lee S.C.
    DNA Cell Biol. 14:529-537(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11.
    Tissue: Liver.
  4. "Coactivator TIF1beta interacts with transcription factor C/EBPbeta and glucocorticoid receptor to induce alpha1-acid glycoprotein gene expression."
    Chang C.J., Chen Y.L., Lee S.C.
    Mol. Cell. Biol. 18:5880-5887(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TRIM28.
  5. "Delta-interacting protein A, a new inhibitory partner of CCAAT/enhancer-binding protein beta, implicated in adipocyte differentiation."
    Bezy O., Elabd C., Cochet O., Petersen R.K., Kristiansen K., Dani C., Ailhaud G., Amri E.-Z.
    J. Biol. Chem. 280:11432-11438(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CCDC85B.
  6. "IFN-gamma-stimulated transcriptional activation by IFN-gamma-activated transcriptional element-binding factor 1 occurs via an inducible interaction with CAAAT/enhancer-binding protein-beta."
    Meng Q., Raha A., Roy S., Hu J., Kalvakolanu D.V.
    J. Immunol. 174:6203-6211(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PTGES2.
  7. "THOC5 couples M-CSF receptor signaling to transcription factor expression."
    Carney L., Pierce A., Rijnen M., Gonzalez Sanchez M.B., Hamzah H.G., Zhang L., Tamura T., Whetton A.D.
    Cell. Signal. 21:309-316(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, COMPLEX FORMATION WITH THOC5.
  8. "The orphan nuclear receptor RORalpha restrains adipocyte differentiation through a reduction of C/EBPbeta activity and perilipin gene expression."
    Ohoka N., Kato S., Takahashi Y., Hayashi H., Sato R.
    Mol. Endocrinol. 23:759-771(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN ADIPOGENESIS, INTERACTION WITH EP300 AND RORA, ALTERNATIVE SPLICING (ISOFORMS 2 AND 3), PHOSPHORYLATION AT THR-188.
  9. "Initiation of myoblast to brown fat switch by a PRDM16-C/EBP-beta transcriptional complex."
    Kajimura S., Seale P., Kubota K., Lunsford E., Frangioni J.V., Gygi S.P., Spiegelman B.M.
    Nature 460:1154-1158(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH PRDM16, INDUCTION BY COLD EXPOSURE, TISSUE SPECIFICITY.
  10. "Regulation of adipocyte differentiation by the zinc finger protein ZNF638."
    Meruvu S., Hugendubler L., Mueller E.
    J. Biol. Chem. 286:26516-26523(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ZNF638.
  11. "Cidea is an essential transcriptional coactivator regulating mammary gland secretion of milk lipids."
    Wang W., Lv N., Zhang S., Shui G., Qian H., Zhang J., Chen Y., Ye J., Xie Y., Shen Y., Wenk M.R., Li P.
    Nat. Med. 18:235-243(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CIDEA AND CIDEC.

Entry informationi

Entry nameiCEBPB_MOUSE
AccessioniPrimary (citable) accession number: P28033
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: September 3, 2014
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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