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P28033

- CEBPB_MOUSE

UniProt

P28033 - CEBPB_MOUSE

Protein

CCAAT/enhancer-binding protein beta

Gene

Cebpb

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 140 (01 Oct 2014)
      Sequence version 1 (01 Aug 1992)
      Previous versions | rss
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    Functioni

    Important transcription factor regulating the expression of genes involved in immune and inflammatory responses. Binds to regulatory regions of several acute-phase and cytokines genes and probably plays a role in the regulation of acute-phase reaction, inflammation and hemopoiesis. The consensus recognition site is 5'-T[TG]NNGNAA[TG]-3'. Plays an important role in adipose tissue differentiation. Regulates the transcriptional induction of peroxisome proliferator-activated receptor gamma (PPARG) as well as other adipogenesis key player genes.2 Publications
    Isoform 3: acts as a dominant negative through heterodimerization with isoform 2.By similarity

    GO - Molecular functioni

    1. chromatin binding Source: MGI
    2. DNA binding Source: MGI
    3. protein binding Source: UniProtKB
    4. protein heterodimerization activity Source: UniProtKB
    5. protein homodimerization activity Source: UniProtKB
    6. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: MGI
    7. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Source: UniProtKB
    8. sequence-specific DNA binding Source: MGI

    GO - Biological processi

    1. brown fat cell differentiation Source: UniProtKB
    2. cellular response to amino acid stimulus Source: MGI
    3. embryonic placenta development Source: MGI
    4. fat cell differentiation Source: MGI
    5. mammary gland epithelial cell differentiation Source: MGI
    6. mammary gland epithelial cell proliferation Source: MGI
    7. negative regulation of neuron apoptotic process Source: MGI
    8. negative regulation of transcription, DNA-templated Source: MGI
    9. neuron differentiation Source: MGI
    10. positive regulation of fat cell differentiation Source: UniProtKB
    11. positive regulation of osteoblast differentiation Source: MGI
    12. positive regulation of transcription, DNA-templated Source: MGI
    13. positive regulation of transcription from RNA polymerase II promoter Source: MGI
    14. regulation of interleukin-6 biosynthetic process Source: MGI
    15. regulation of transcription, DNA-templated Source: UniProtKB
    16. regulation of transcription involved in cell fate commitment Source: UniProtKB
    17. response to endoplasmic reticulum stress Source: UniProtKB
    18. response to lipopolysaccharide Source: BHF-UCL
    19. transcription from RNA polymerase II promoter Source: GOC

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Differentiation, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_206033. Senescence-Associated Secretory Phenotype (SASP).
    REACT_27166. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    CCAAT/enhancer-binding protein beta
    Short name:
    C/EBP beta
    Alternative name(s):
    AGP/EBP
    Interleukin-6-dependent-binding protein
    Short name:
    IL-6DBP
    Liver-enriched transcriptional activator
    Short name:
    LAP
    Gene namesi
    Name:Cebpb
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:88373. Cebpb.

    Subcellular locationi

    Nucleus 1 PublicationPROSITE-ProRule annotation

    GO - Cellular componenti

    1. condensed chromosome, centromeric region Source: MGI
    2. cytoplasm Source: MGI
    3. nuclear chromatin Source: BHF-UCL
    4. nuclear matrix Source: MGI
    5. nucleoplasm Source: Reactome
    6. nucleus Source: MGI

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Mice die in 24 hours after birth. Embryos display defects in brown fat tissue development.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 296296CCAAT/enhancer-binding protein betaPRO_0000076618Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki133 – 133Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
    Modified residuei188 – 1881Phosphothreonine; by RPS6KA11 Publication

    Post-translational modificationi

    Sumoylated by polymeric chains of SUMO2 or SUMO3.By similarity

    Keywords - PTMi

    Isopeptide bond, Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiP28033.
    PRIDEiP28033.

    PTM databases

    PhosphoSiteiP28033.

    Expressioni

    Tissue specificityi

    Abundantly expressed in myoblasts. Enriched in brown adipose tissue (BAT) versus white adipose tissue (WAT).1 Publication

    Inductioni

    Up-regulated by cold exposure.1 Publication

    Gene expression databases

    ArrayExpressiP28033.
    BgeeiP28033.
    CleanExiMM_CEBPB.
    GenevestigatoriP28033.

    Interactioni

    Subunit structurei

    Binds DNA as a dimer and can form stable heterodimers with CEBPA, CEBPD and CEBPG. Interacts with TRIM28 and PTGES2. Interacts with PRDM16. Interacts with CCDC85B. Forms a complex with THOC5. Interacts with ZNF638; this interaction increases transcriptional activation. Interacts with CIDEA and CIDEC. Interaction with CIDEA increases transcriptional activation of a subset of CEBPB downstream target genes, including ID2, IGF1, PRLR, SOCS1, SOCS3, XDH. Interaction with CIDEC increases transcriptional activation of SOCS1, SOCS3, TGFB1, TGFBR1, ID2 and XDH. Interacts with DDIT3/CHOP. Interacts with EP300; recruits EP300 to chromatin. Interacts with RORA; the interaction disrupts interaction with EP300.7 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Bhlhe41Q99PV55EBI-1029979,EBI-6143801

    Protein-protein interaction databases

    BioGridi198669. 12 interactions.
    DIPiDIP-37539N.
    IntActiP28033. 7 interactions.
    STRINGi10090.ENSMUSP00000069850.

    Structurei

    Secondary structure

    1
    296
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi240 – 28243

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1CI6X-ray2.60B224-285[»]
    ProteinModelPortaliP28033.
    SMRiP28033. Positions 239-285.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP28033.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini222 – 28564bZIPPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 2222Required for Lys-174 sumoylationBy similarityAdd
    BLAST
    Regioni226 – 24621Basic motifPROSITE-ProRule annotationAdd
    BLAST
    Regioni248 – 2558Leucine-zipperPROSITE-ProRule annotation

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi120 – 12910Pro-rich
    Compositional biasi170 – 19122Pro/Ser-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the bZIP family. C/EBP subfamily.Curated
    Contains 1 bZIP (basic-leucine zipper) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG299311.
    HOGENOMiHOG000013112.
    HOVERGENiHBG050879.
    InParanoidiP28033.
    KOiK10048.
    OMAiCKKPAEY.
    OrthoDBiEOG7R56TQ.
    PhylomeDBiP28033.
    TreeFamiTF105008.

    Family and domain databases

    InterProiIPR004827. bZIP.
    IPR016468. CCAAT/enhancer-binding.
    [Graphical view]
    PfamiPF07716. bZIP_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005879. CCAAT/enhancer-binding. 1 hit.
    SMARTiSM00338. BRLZ. 1 hit.
    [Graphical view]
    PROSITEiPS50217. BZIP. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative initiation. Align

    Isoform 1 (identifier: P28033-1) [UniParc]FASTAAdd to Basket

    Also known as: a, C/EBPbeta-FL

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MHRLLAWDAA CLPPPPAAFR PMEVANFYYE PDCLAYGAKA ARAAPRAPAA    50
    EPAIGEHERA IDFSPYLEPL APAADFAAPA PAHHDFLSDL FADDYGAKPS 100
    KKPADYGYVS LGRAGAKAAP PACFPPPPPA ALKAEPGFEP ADCKRADDAP 150
    AMAAGFPFAL RAYLGYQATP SGSSGSLSTS SSSSPPGTPS PADAKAAPAA 200
    CFAGPPAAPA KAKAKKTVDK LSDEYKMRRE RNNIAVRKSR DKAKMRNLET 250
    QHKVLELTAE NERLQKKVEQ LSRELSTLRN LFKQLPEPLL ASAGHC 296
    Length:296
    Mass (Da):31,446
    Last modified:August 1, 1992 - v1
    Checksum:i827AC4AFC209AE89
    GO
    Isoform 2 (identifier: P28033-3) [UniParc]FASTAAdd to Basket

    Also known as: b, C/EBPbeta-LAP

    The sequence of this isoform differs from the canonical sequence as follows:
         1-21: Missing.

    Note: Major isoform.

    Show »
    Length:275
    Mass (Da):29,133
    Checksum:i717E36841E48752D
    GO
    Isoform 3 (identifier: P28033-2) [UniParc]FASTAAdd to Basket

    Also known as: c, C/EBPbeta-LIP

    The sequence of this isoform differs from the canonical sequence as follows:
         1-151: Missing.

    Show »
    Length:145
    Mass (Da):15,597
    Checksum:iC7AFEEE4F98F5978
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 151151Missing in isoform 3. CuratedVSP_053976Add
    BLAST
    Alternative sequencei1 – 2121Missing in isoform 2. CuratedVSP_053977Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M61007 mRNA. Translation: AAA37192.1.
    X62600 mRNA. Translation: CAA44484.1.
    S78572 Genomic DNA. No translation available.
    CCDSiCCDS17105.1. [P28033-1]
    PIRiA36366.
    RefSeqiNP_001274667.1. NM_001287738.1. [P28033-3]
    NP_001274668.1. NM_001287739.1. [P28033-2]
    NP_034013.1. NM_009883.4. [P28033-1]
    UniGeneiMm.439656.

    Genome annotation databases

    EnsembliENSMUST00000070642; ENSMUSP00000069850; ENSMUSG00000056501. [P28033-1]
    GeneIDi12608.
    KEGGimmu:12608.
    UCSCiuc008oaf.1. mouse. [P28033-1]

    Keywords - Coding sequence diversityi

    Alternative initiation

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M61007 mRNA. Translation: AAA37192.1 .
    X62600 mRNA. Translation: CAA44484.1 .
    S78572 Genomic DNA. No translation available.
    CCDSi CCDS17105.1. [P28033-1 ]
    PIRi A36366.
    RefSeqi NP_001274667.1. NM_001287738.1. [P28033-3 ]
    NP_001274668.1. NM_001287739.1. [P28033-2 ]
    NP_034013.1. NM_009883.4. [P28033-1 ]
    UniGenei Mm.439656.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1CI6 X-ray 2.60 B 224-285 [» ]
    ProteinModelPortali P28033.
    SMRi P28033. Positions 239-285.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 198669. 12 interactions.
    DIPi DIP-37539N.
    IntActi P28033. 7 interactions.
    STRINGi 10090.ENSMUSP00000069850.

    PTM databases

    PhosphoSitei P28033.

    Proteomic databases

    MaxQBi P28033.
    PRIDEi P28033.

    Protocols and materials databases

    DNASUi 12608.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000070642 ; ENSMUSP00000069850 ; ENSMUSG00000056501 . [P28033-1 ]
    GeneIDi 12608.
    KEGGi mmu:12608.
    UCSCi uc008oaf.1. mouse. [P28033-1 ]

    Organism-specific databases

    CTDi 1051.
    MGIi MGI:88373. Cebpb.

    Phylogenomic databases

    eggNOGi NOG299311.
    HOGENOMi HOG000013112.
    HOVERGENi HBG050879.
    InParanoidi P28033.
    KOi K10048.
    OMAi CKKPAEY.
    OrthoDBi EOG7R56TQ.
    PhylomeDBi P28033.
    TreeFami TF105008.

    Enzyme and pathway databases

    Reactomei REACT_206033. Senescence-Associated Secretory Phenotype (SASP).
    REACT_27166. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

    Miscellaneous databases

    ChiTaRSi CEBPB. mouse.
    EvolutionaryTracei P28033.
    NextBioi 281758.
    PROi P28033.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P28033.
    Bgeei P28033.
    CleanExi MM_CEBPB.
    Genevestigatori P28033.

    Family and domain databases

    InterProi IPR004827. bZIP.
    IPR016468. CCAAT/enhancer-binding.
    [Graphical view ]
    Pfami PF07716. bZIP_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005879. CCAAT/enhancer-binding. 1 hit.
    SMARTi SM00338. BRLZ. 1 hit.
    [Graphical view ]
    PROSITEi PS50217. BZIP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of a transcription factor, AGP/EBP, that belongs to members of the C/EBP family."
      Chang C.J., Chen T.T., Lei H.Y., Chen D.S., Lee S.C.
      Mol. Cell. Biol. 10:6642-6653(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
      Tissue: Liver.
    2. "Regulated expression of three C/EBP isoforms during adipose conversion of 3T3-L1 cells."
      Cao Z., Umek R.M., McKnight S.L.
      Genes Dev. 5:1538-1552(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Autoregulated induction of the acute-phase response transcription factor gene, agp/ebp."
      Chang C.J., Shen B.J., Lee S.C.
      DNA Cell Biol. 14:529-537(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11.
      Tissue: Liver.
    4. "Coactivator TIF1beta interacts with transcription factor C/EBPbeta and glucocorticoid receptor to induce alpha1-acid glycoprotein gene expression."
      Chang C.J., Chen Y.L., Lee S.C.
      Mol. Cell. Biol. 18:5880-5887(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH TRIM28.
    5. "Delta-interacting protein A, a new inhibitory partner of CCAAT/enhancer-binding protein beta, implicated in adipocyte differentiation."
      Bezy O., Elabd C., Cochet O., Petersen R.K., Kristiansen K., Dani C., Ailhaud G., Amri E.-Z.
      J. Biol. Chem. 280:11432-11438(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CCDC85B.
    6. "IFN-gamma-stimulated transcriptional activation by IFN-gamma-activated transcriptional element-binding factor 1 occurs via an inducible interaction with CAAAT/enhancer-binding protein-beta."
      Meng Q., Raha A., Roy S., Hu J., Kalvakolanu D.V.
      J. Immunol. 174:6203-6211(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PTGES2.
    7. "THOC5 couples M-CSF receptor signaling to transcription factor expression."
      Carney L., Pierce A., Rijnen M., Gonzalez Sanchez M.B., Hamzah H.G., Zhang L., Tamura T., Whetton A.D.
      Cell. Signal. 21:309-316(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, COMPLEX FORMATION WITH THOC5.
    8. "The orphan nuclear receptor RORalpha restrains adipocyte differentiation through a reduction of C/EBPbeta activity and perilipin gene expression."
      Ohoka N., Kato S., Takahashi Y., Hayashi H., Sato R.
      Mol. Endocrinol. 23:759-771(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN ADIPOGENESIS, INTERACTION WITH EP300 AND RORA, ALTERNATIVE SPLICING (ISOFORMS 2 AND 3), PHOSPHORYLATION AT THR-188.
    9. "Initiation of myoblast to brown fat switch by a PRDM16-C/EBP-beta transcriptional complex."
      Kajimura S., Seale P., Kubota K., Lunsford E., Frangioni J.V., Gygi S.P., Spiegelman B.M.
      Nature 460:1154-1158(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH PRDM16, INDUCTION BY COLD EXPOSURE, TISSUE SPECIFICITY.
    10. "Regulation of adipocyte differentiation by the zinc finger protein ZNF638."
      Meruvu S., Hugendubler L., Mueller E.
      J. Biol. Chem. 286:26516-26523(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ZNF638.
    11. "Cidea is an essential transcriptional coactivator regulating mammary gland secretion of milk lipids."
      Wang W., Lv N., Zhang S., Shui G., Qian H., Zhang J., Chen Y., Ye J., Xie Y., Shen Y., Wenk M.R., Li P.
      Nat. Med. 18:235-243(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CIDEA AND CIDEC.

    Entry informationi

    Entry nameiCEBPB_MOUSE
    AccessioniPrimary (citable) accession number: P28033
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: August 1, 1992
    Last modified: October 1, 2014
    This is version 140 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3