Reviewed, UniProtKB/Swiss-Prot P28033 (CEBPB_MOUSE)

Names and origin

Protein names

Recommended name:
    CCAAT/enhancer-binding protein beta
      Short name=C/EBP beta
Alternative name(s):
    Interleukin-6-dependent-binding protein
      Short name=IL-6DBP
    Liver-enriched transcriptional activator
      Short name=LAP
    AGP/EBP

Gene names

Name:

Cebpb

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	296 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in interleukin-6 signal transduction, including the transcriptional activation of acute-phase genes. Functions in brown adipose tissue (BAT) differentiation. Ref.7
Subunit structure	Binds DNA as a dimer and can form stable heterodimers with C/EBP alpha and gamma. Interacts with TRIM28 and PTGES2. Interacts with PRDM16. Interacts with CCDC85B. Ref.7 Ref.4 Ref.5 Ref.6
Subcellular location	Nucleus.
Tissue specificity	Abundantly expressed in myoblasts. Enriched in brown adipose tissue (BAT) versus white adipose tissue (WAT). Ref.7
Induction	Up-regulated by cold exposure. Ref.7
Disruption phenotype	Mice die in 24 hours after birth. Embryos display defects in brown fat tissue development. Ref.7
Sequence similarities	Belongs to the bZIP family. C/EBP subfamily. Contains 1 bZIP domain.

Ontologies

Keywords
Biological process	Differentiation Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Activator
PTM	Isopeptide bond Ubl conjugation
Technical term	3D-structure
Gene Ontology (GO)
Biological process	anti-apoptosis Inferred from direct assay. Source: MGI brown fat cell differentiation Ref.7 Inferred from mutant phenotype. Source: UniProtKB embryonic placenta development Inferred from genetic interaction. Source: MGI induction of apoptosis Inferred from direct assay. Source: MGI mammary gland epithelial cell differentiation Inferred from mutant phenotype. Source: MGI mammary gland epithelial cell proliferation Inferred from mutant phenotype. Source: MGI neuron differentiation Inferred from direct assay. Source: MGI positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay. Source: MGI regulation of interleukin-6 biosynthetic process Inferred from direct assay. Source: MGI transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from direct assay. Source: MGI nucleus Inferred from direct assay. Source: MGI
Molecular function	RNA polymerase II transcription factor activity, enhancer binding Ref.7 Inferred from direct assay. Source: UniProtKB protein heterodimerization activity Inferred from direct assay. Source: UniProtKB protein homodimerization activity Inferred from direct assay. Source: UniProtKB sequence-specific DNA binding Inferred from direct assay. Source: MGI transcription activator activity Inferred from direct assay. Source: MGI
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ATF4	P18848	1	EBI-1029979,EBI-492498	From a different organism.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 296

296

CCAAT/enhancer-binding protein beta

PRO_0000076618

Regions

Domain

257 – 285

29

Leucine-zipper

DNA binding

228 – 244

17

Basic motif

Region

1 – 22

22

Required for Lys-174 sumoylation By similarity

Compositional bias

120 – 129

10

Pro-rich

Compositional bias

170 – 191

22

Pro/Ser-rich

Amino acid modifications

Cross-link

133

Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity

Secondary structure

1

.

.

.

296

Helix Strand Turn

Details...

Helix

240 – 282

43

Sequences

	Sequence		Length	Mass (Da)
	P28033-1 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 827AC4AFC209AE89	FASTA	296	31,446
	10 20 30 40 50 60 MHRLLAWDAA CLPPPPAAFR PMEVANFYYE PDCLAYGAKA ARAAPRAPAA EPAIGEHERA 70 80 90 100 110 120 IDFSPYLEPL APAADFAAPA PAHHDFLSDL FADDYGAKPS KKPADYGYVS LGRAGAKAAP 130 140 150 160 170 180 PACFPPPPPA ALKAEPGFEP ADCKRADDAP AMAAGFPFAL RAYLGYQATP SGSSGSLSTS 190 200 210 220 230 240 SSSSPPGTPS PADAKAAPAA CFAGPPAAPA KAKAKKTVDK LSDEYKMRRE RNNIAVRKSR 250 260 270 280 290 DKAKMRNLET QHKVLELTAE NERLQKKVEQ LSRELSTLRN LFKQLPEPLL ASAGHC

References

[1]	"Molecular cloning of a transcription factor, AGP/EBP, that belongs to members of the C/EBP family." Chang C.J., Chen T.T., Lei H.Y., Chen D.S., Lee S.C. Mol. Cell. Biol. 10:6642-6653(1990) [PubMed: 1701020] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Liver.
[2]	"Regulated expression of three C/EBP isoforms during adipose conversion of 3T3-L1 cells." Cao Z., Umek R.M., McKnight S.L. Genes Dev. 5:1538-1552(1991) [PubMed: 1840554] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[3]	"Autoregulated induction of the acute-phase response transcription factor gene, agp/ebp." Chang C.J., Shen B.J., Lee S.C. DNA Cell Biol. 14:529-537(1995) [PubMed: 7598808] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11. Tissue: Liver.
[4]	"Coactivator TIF1beta interacts with transcription factor C/EBPbeta and glucocorticoid receptor to induce alpha1-acid glycoprotein gene expression." Chang C.J., Chen Y.L., Lee S.C. Mol. Cell. Biol. 18:5880-5887(1998) [PubMed: 9742105] [Abstract] Cited for: INTERACTION WITH TRIM28.
[5]	"Delta-interacting protein A, a new inhibitory partner of CCAAT/enhancer-binding protein beta, implicated in adipocyte differentiation." Bezy O., Elabd C., Cochet O., Petersen R.K., Kristiansen K., Dani C., Ailhaud G., Amri E.-Z. J. Biol. Chem. 280:11432-11438(2005) [PubMed: 15644333] [Abstract] Cited for: INTERACTION WITH CCDC85B.
[6]	"IFN-gamma-stimulated transcriptional activation by IFN-gamma-activated transcriptional element-binding factor 1 occurs via an inducible interaction with CAAAT/enhancer-binding protein-beta." Meng Q., Raha A., Roy S., Hu J., Kalvakolanu D.V. J. Immunol. 174:6203-6211(2005) [PubMed: 15879117] [Abstract] Cited for: INTERACTION WITH PTGES2.
[7]	"Initiation of myoblast to brown fat switch by a PRDM16-C/EBP-beta transcriptional complex." Kajimura S., Seale P., Kubota K., Lunsford E., Frangioni J.V., Gygi S.P., Spiegelman B.M. Nature 460:1154-1158(2009) [PubMed: 19641492] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH PRDM16, INDUCTION BY COLD EXPOSURE, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X62600 mRNA. Translation: CAA44484.1.
M61007 mRNA. Translation: AAA37192.1.
S78572 Genomic DNA. No translation available.

IPI

IPI00116613.

PIR

A36366.

RefSeq

NP_034013.1.

UniGene

Mm.439656

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1CI6	X-ray	2.60	B	224-285	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P28033. 1 interaction.

STRING

P28033.

PTM databases

PhosphoSite

P28033.

Proteomic databases

PRIDE

P28033.

Genome annotation databases

Ensembl

ENSMUST00000070642; ENSMUSP00000069850; ENSMUSG00000056501; Mus musculus. [Genome view]

GeneID

12608.

KEGG

mmu:12608.

UCSC

uc008oaf.1. mouse.

Organism-specific databases

CTD

12608.

MGI

MGI:88373. Cebpb.

Phylogenomic databases

HOGENOM

P28033.

HOVERGEN

P28033.

OMA

KAPPAAC.

Gene expression databases

ArrayExpress

P28033.

Bgee

P28033.

CleanEx

MM_CEBPB.

Genevestigator

P28033.

GermOnline

ENSMUSG00000056501. Mus musculus.

Family and domain databases

InterPro

IPR011700. bZIP_2.
IPR016468. CCAAT/enhancer-binding.
IPR004827. TF_bZIP.
[Graphical view]

Pfam

PF07716. bZIP_2. 1 hit.
[Graphical view]

PIRSF

PIRSF005879. CCAAT/enhancer-binding. 1 hit.

SMART

SM00338. BRLZ. 1 hit.
[Graphical view]

PROSITE

PS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. False negative.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

281758.

SOURCE

Search...

Entry information

Entry name

CEBPB_MOUSE

Accession

Primary (citable) accession number: P28033

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	November 3, 2009
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)