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Reviewed, UniProtKB/Swiss-Prot P28032 (ADH2_SOLLC)

Last modified September 22, 2009. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Alcohol dehydrogenase 2
    EC=1.1.1.1
Gene names
Name: ADH2
OrganismSolanum lycopersicum (Tomato) (Lycopersicon esculentum)
Taxonomic identifier4081 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum › Lycopersicon

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Protein attributes

Sequence length380 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Catalytic activity

An alcohol + NAD+ = an aldehyde or ketone + NADH.

Cofactor

Binds 2 zinc ions per subunit By similarity.

Subunit structure

Homotetramer.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the zinc-containing alcohol dehydrogenase family.

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Ontologies

Keywords
   Cellular componentCytoplasm
   LigandMetal-binding
NAD
Zinc
   Molecular functionOxidoreductase
Gene Ontology (GO)
   Biological processoxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionalcohol dehydrogenase (NAD) activity

Inferred from electronic annotation. Source: EC

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 380380Alcohol dehydrogenase 2
PRO_0000160702

Regions

Nucleotide binding203 – 2086NAD By similarity
Nucleotide binding296 – 2983NAD By similarity

Sites

Metal binding481Zinc 1; catalytic By similarity
Metal binding701Zinc 1; catalytic By similarity
Metal binding1001Zinc 2 By similarity
Metal binding1031Zinc 2 By similarity
Metal binding1061Zinc 2 By similarity
Metal binding1141Zinc 2 By similarity
Metal binding1781Zinc 1; catalytic By similarity
Binding site2271NAD By similarity
Binding site2321NAD By similarity
Binding site3731NAD By similarity

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Sequences

Sequence LengthMass (Da)Tools
P28032-1 [UniParc].

Last modified July 1, 1993. Version 2.
Checksum: D5B123DF9DE28027

FASTA38041,040
        10         20         30         40         50         60 
MSTTVGQVIR CKAAVAWEAG KPLVMEEVDV APPQKMEVRL KILYTSLCHT DVYFWEAKGQ 

        70         80         90        100        110        120 
NPVFPRILGH EAAGIVESVG EGVTDLAPGD HVLPVFTGEC KDCAHCKSEE SNMCSLLRIN 

       130        140        150        160        170        180 
TDRGVMLNDG KSRFSINGNP IYHFVGTSTF SEYTVVHVGC VAKINPLAPL DKVCVLSCGI 

       190        200        210        220        230        240 
STGLGASLNV AKPTKGSSVA IFGLGAVGLA AAEGARIAGA SRIIGVDLNA SRFEQAKKFG 

       250        260        270        280        290        300 
VTEFVNPKDY SKPVQEVIAE MTDGGVDRSV ECTGHIDAMI SAFECVHDGW GVAVLVGVPH 

       310        320        330        340        350        360 
KEAVFKTHPL NFLNERTLKG TFFGNYKPRS DIPCVVEKYM NKELELEKFI THTLPFAEIN 

       370        380 
KAFDLMLKGE GLRCIITMAD

« Hide

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References

[1]"Isolation of a tomato alcohol dehydrogenase 2-encoding cDNA using phage-promoted antibody screening of a plasmid cDNA library."
Genez A.L., Staraci L.C., Alexander D.C., Rejda V.M., Williamson V.M., Chase T. Jr., Williams B.G.
Gene 123:157-164(1993) [PubMed: 8428654] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[2]"Structure of the tomato Adh2 gene and Adh2 pseudogenes, and a study of Adh2 gene expression in fruit."
Longhurst T., Lee E., Hinde R., Brady C., Speirs J.
Plant Mol. Biol. 26:1073-1084(1994) [PubMed: 7811967] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Strain: cv. De Ruiter 83G38.
[3]"Tomato alcohol dehydrogenase. Expression during fruit ripening and under hypoxic conditions."
van der Straeten D., Pousada R.A.R., Gielen J., van Montagu M.
FEBS Lett. 295:39-42(1991) [PubMed: 1765164] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 252-380.
Strain: cv. Orlando.

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Cross-references

Sequence databases

M86724 mRNA. Translation: AAA34133.1.
X77233 Genomic DNA. Translation: CAA54450.1.
X60600 mRNA. Translation: CAA43055.1.
PIRS51826.
UniGeneLes.19427

3D structure databases

HSSPHSSP built from PDB template 1M6H based on UniProtKB P11766.
ModBaseSearch...

Enzyme and pathway databases

BRENDA1.1.1.1. 281054.

Family and domain databases

InterProIPR013154. ADH_GroES-like.
IPR002085. ADH_SF_Zn.
IPR013149. ADH_Zn-bd.
IPR002328. ADH_Zn_CS.
[Graphical view]
PANTHERPTHR11695. ADH_Sf_Zn. 1 hit.
PfamPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
PROSITEPS00059. ADH_ZINC. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameADH2_SOLLC
AccessionPrimary (citable) accession number: P28032
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: July 1, 1993
Last modified: September 22, 2009
This is version 66 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents