Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Serine/threonine-protein kinase B-raf

Gene

Braf

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Zn2+CuratedNote: Binds 2 Zn2+ ions per subunit.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi219Zinc 1By similarity1
Metal bindingi232Zinc 2By similarity1
Metal bindingi235Zinc 2By similarity1
Metal bindingi245Zinc 1By similarity1
Metal bindingi248Zinc 1By similarity1
Metal bindingi253Zinc 2By similarity1
Metal bindingi256Zinc 2By similarity1
Metal bindingi264Zinc 1By similarity1
Binding sitei520ATPPROSITE-ProRule annotation1
Active sitei613Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi500 – 508ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • alpha-beta T cell differentiation Source: MGI
  • CD4-positive, alpha-beta T cell differentiation Source: MGI
  • CD4-positive or CD8-positive, alpha-beta T cell lineage commitment Source: MGI
  • cell differentiation Source: MGI
  • cellular response to calcium ion Source: MGI
  • cellular response to drug Source: MGI
  • cellular response to nerve growth factor stimulus Source: Ensembl
  • establishment of protein localization to membrane Source: MGI
  • face development Source: MGI
  • head morphogenesis Source: MGI
  • long-term synaptic potentiation Source: MGI
  • MAPK cascade Source: MGI
  • myeloid progenitor cell differentiation Source: MGI
  • negative regulation of apoptotic process Source: MGI
  • negative regulation of endothelial cell apoptotic process Source: MGI
  • negative regulation of fibroblast migration Source: MGI
  • negative regulation of neuron apoptotic process Source: MGI
  • negative regulation of signal transduction Source: GO_Central
  • negative regulation of synaptic vesicle exocytosis Source: MGI
  • positive regulation of axonogenesis Source: MGI
  • positive regulation of axon regeneration Source: MGI
  • positive regulation of ERK1 and ERK2 cascade Source: MGI
  • positive regulation of gene expression Source: MGI
  • positive regulation of glucose transmembrane transport Source: MGI
  • positive regulation of peptidyl-serine phosphorylation Source: MGI
  • positive regulation of stress fiber assembly Source: MGI
  • positive regulation of substrate adhesion-dependent cell spreading Source: MGI
  • positive T cell selection Source: MGI
  • protein heterooligomerization Source: MGI
  • protein phosphorylation Source: BHF-UCL
  • regulation of axon regeneration Source: MGI
  • regulation of cell proliferation Source: MGI
  • regulation of T cell differentiation Source: MGI
  • response to cAMP Source: MGI
  • response to peptide hormone Source: MGI
  • somatic stem cell population maintenance Source: MGI
  • T cell receptor signaling pathway Source: MGI
  • thymus development Source: MGI
  • thyroid gland development Source: MGI
  • trehalose metabolism in response to stress Source: MGI
  • visual learning Source: MGI

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-1295596 Spry regulation of FGF signaling
R-MMU-442742 CREB phosphorylation through the activation of Ras
R-MMU-5673000 RAF activation
R-MMU-5674135 MAP2K and MAPK activation
R-MMU-5674499 Negative feedback regulation of MAPK pathway
R-MMU-5675221 Negative regulation of MAPK pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase B-raf (EC:2.7.11.1)
Alternative name(s):
Proto-oncogene B-Raf
Gene namesi
Name:Braf
Synonyms:B-raf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:88190 Braf

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

Pathology & Biotechi

Involvement in diseasei

Participates in a chromosomal translocation that produces a Tif1a-BRAF (T18) oncogene originally isolated from a furfural-induced hepatoma.

Keywords - Diseasei

Proto-oncogene

Chemistry databases

ChEMBLiCHEMBL2331061

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000856662 – 804Serine/threonine-protein kinase B-rafAdd BLAST803

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei135PhosphoserineBy similarity1
Modified residuei316PhosphoserineCombined sources1
Modified residuei348PhosphoserineCombined sources1
Modified residuei356Phosphothreonine; by autocatalysisBy similarity1
Modified residuei431PhosphothreonineBy similarity1
Modified residuei434PhosphoserineBy similarity1
Modified residuei436PhosphothreonineBy similarity1
Modified residuei483PhosphoserineCombined sources1
Modified residuei484PhosphoserineCombined sources1
Cross-linki615Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei708Omega-N-methylarginine; by PRMT5By similarity1
Modified residuei766PhosphoserineBy similarity1
Modified residuei787PhosphoserineCombined sources1
Modified residuei790Phosphothreonine; by MAPK1By similarity1

Post-translational modificationi

Phosphorylation at Ser-348 by SGK1 inhibits its activity.By similarity
Methylation by PRMT5 decreases stability and kinase activity.1 Publication
Ubiquitinated by RNF149; which leads to proteasomal degradation. Polyubiquitinated at Lys-615 in response to EGF (By similarity).By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP28028
MaxQBiP28028
PaxDbiP28028
PeptideAtlasiP28028
PRIDEiP28028

PTM databases

iPTMnetiP28028
PhosphoSitePlusiP28028

Expressioni

Gene expression databases

BgeeiENSMUSG00000002413
CleanExiMM_BRAF
ExpressionAtlasiP28028 baseline and differential
GenevisibleiP28028 MM

Interactioni

Subunit structurei

Monomer. Homodimer. Heterodimerizes with RAF1, and the heterodimer possesses a highly increased kinase activity compared to the respective homodimers or monomers. Heterodimerization is mitogen-regulated and enhanced by 14-3-3 proteins. MAPK1/ERK2 activation can induce a negative feedback that promotes the dissociation of the heterodimer by phosphorylating BRAF at Thr-790. Found in a complex with at least BRAF, HRAS, MAP2K1, MAPK3 and RGS14. Interacts with RIT1. Interacts (via N-terminus) with RGS14 (via RBD domains); the interaction mediates the formation of a ternary complex with RAF1, a ternary complex inhibited by GNAI1 (By similarity). Interacts with DGKH (By similarity). Interacts with PRMT5 (By similarity). Interacts with AKAP13, MAP2K1 and KSR1. Identified in a complex with AKAP13, KSR1 and MAP2K1 (PubMed:21102438). Interacts with FNIP1 and FNIP2 (By similarity).By similarity1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi225124, 6 interactors
CORUMiP28028
DIPiDIP-57050N
IntActiP28028, 10 interactors
MINTiP28028
STRINGi10090.ENSMUSP00000002487

Structurei

3D structure databases

ProteinModelPortaliP28028
SMRiP28028
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini139 – 211RBDPROSITE-ProRule annotationAdd BLAST73
Domaini494 – 754Protein kinasePROSITE-ProRule annotationAdd BLAST261

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi6 – 30Poly-GlyAdd BLAST25
Compositional biasi465 – 469Poly-Ser5

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0193 Eukaryota
ENOG410Y4UP LUCA
GeneTreeiENSGT00900000140880
HOGENOMiHOG000252972
HOVERGENiHBG001886
InParanoidiP28028
KOiK04365
OMAiIVFDFEP
OrthoDBiEOG091G09SB
TreeFamiTF317006

Family and domain databases

CDDicd00029 C1, 1 hit
InterProiView protein in InterPro
IPR020454 DAG/PE-bd
IPR011009 Kinase-like_dom_sf
IPR002219 PE/DAG-bd
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR003116 RBD_dom
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
IPR008271 Ser/Thr_kinase_AS
IPR029071 Ubiquitin-like_domsf
PfamiView protein in Pfam
PF00130 C1_1, 1 hit
PF07714 Pkinase_Tyr, 1 hit
PF02196 RBD, 1 hit
PRINTSiPR00008 DAGPEDOMAIN
SMARTiView protein in SMART
SM00109 C1, 1 hit
SM00455 RBD, 1 hit
SM00220 S_TKc, 1 hit
SUPFAMiSSF54236 SSF54236, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
PS50898 RBD, 1 hit
PS00479 ZF_DAG_PE_1, 1 hit
PS50081 ZF_DAG_PE_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P28028-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAALSGGGGS SSGGGGGGGG GGGGGDGGGG AEQGQALFNG DMEPEAGAGA
60 70 80 90 100
AASSAADPAI PEEVWNIKQM IKLTQEHIEA LLDKFGGEHN PPSIYLEAYE
110 120 130 140 150
EYTSKLDALQ QREQQLLESL VFQTPTDASR NNPKSPQKPI VRVFLPNKQR
160 170 180 190 200
TVVPARCGVT VRDSLKKALM MRGLIPECCA VYRIQDGEKK PIGWDTDISW
210 220 230 240 250
LTGEELHVEV LENVPLTTHN FVRKTFFTLA FCDFCRKLLF QGFRCQTCGY
260 270 280 290 300
KFHQRCSTEV PLMCVNYDQL DLLFVSKFFE HHPVPQEEAS FPETALPSGS
310 320 330 340 350
SSAPPSDSTG PQILTSPSPS KSIPIPQPFR PADEDHRNQF GQRDRSSSAP
360 370 380 390 400
NVHINTIEPV NIDEKFPEVE LQDQRDLIRD QGFRGDGAPL NQLMRCLRKY
410 420 430 440 450
QSRTPSPLLH SVPSEIVFDF EPGPVFRGST TGLSATPPAS LPGSLTNVKA
460 470 480 490 500
LQKSPGPQRE RKSSSSSSSE DRSRMKTLGR RDSSDDWEIP DGQITVGQRI
510 520 530 540 550
GSGSFGTVYK GKWHGDVAVK MLNVTAPTPQ QLQAFKNEVG VLRKTRHVNI
560 570 580 590 600
LLFMGYSTKP QLAIVTQWCE GSSLYHHLHI IETKFEMIKL IDIARQTAQG
610 620 630 640 650
MDYLHAKSII HRDLKSNNIF LHEDLTVKIG DFGLATVKSR WSGSHQFEQL
660 670 680 690 700
SGSILWMAPE VIRMQDKNPY SFQSDVYAFG IVLYELMTGQ LPYSNINNRD
710 720 730 740 750
QIIFMVGRGY LSPDLSKVRS NCPKAMKRLM AECLKKKRDE RPLFPQILAS
760 770 780 790 800
IELLARSLPK IHRSASEPSL NRAGFQTEDF SLYACASPKT PIQAGGYGEF

AAFK
Length:804
Mass (Da):88,780
Last modified:July 27, 2011 - v3
Checksum:i4C6CC16A2C660A17
GO
Isoform 2 (identifier: P28028-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-474: Missing.
     799-804: EFAAFK → GFPVH

Note: No experimental confirmation available.
Show »
Length:329
Mass (Da):37,118
Checksum:i93913670A27EAB2C
GO

Sequence cautioni

The sequence AAA37320 differs from that shown. Chimeric cDNA.Curated
The sequence AAA37320 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti10 – 11SS → RR in BAE24384 (PubMed:16141072).Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0221691 – 474Missing in isoform 2. 1 PublicationAdd BLAST474
Alternative sequenceiVSP_022170799 – 804EFAAFK → GFPVH in isoform 2. 1 Publication6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK140431 mRNA Translation: BAE24384.1
AC122345 Genomic DNA No translation available.
AC163109 Genomic DNA No translation available.
M64429 mRNA Translation: AAA37320.1 Sequence problems.
CCDSiCCDS39463.1 [P28028-1]
PIRiA40951 TVMSBF
RefSeqiNP_647455.3, NM_139294.5 [P28028-1]
UniGeneiMm.245513
Mm.489691

Genome annotation databases

EnsembliENSMUST00000002487; ENSMUSP00000002487; ENSMUSG00000002413 [P28028-1]
GeneIDi109880
KEGGimmu:109880
UCSCiuc009bme.2 mouse [P28028-1]
uc009bmf.1 mouse [P28028-2]

Keywords - Coding sequence diversityi

Alternative splicing, Chromosomal rearrangement

Similar proteinsi

Entry informationi

Entry nameiBRAF_MOUSE
AccessioniPrimary (citable) accession number: P28028
Secondary accession number(s): E9QNG9, Q3USE9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: July 27, 2011
Last modified: June 20, 2018
This is version 182 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health