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Protein

Serine/threonine-protein kinase B-raf

Gene

Braf

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Zn2+CuratedNote: Binds 2 Zn2+ ions per subunit.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi219Zinc 1By similarity1
Metal bindingi232Zinc 2By similarity1
Metal bindingi235Zinc 2By similarity1
Metal bindingi245Zinc 1By similarity1
Metal bindingi248Zinc 1By similarity1
Metal bindingi253Zinc 2By similarity1
Metal bindingi256Zinc 2By similarity1
Metal bindingi264Zinc 1By similarity1
Binding sitei520ATPPROSITE-ProRule annotation1
Active sitei613Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi500 – 508ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • alpha-beta T cell differentiation Source: MGI
  • CD4-positive, alpha-beta T cell differentiation Source: MGI
  • CD4-positive or CD8-positive, alpha-beta T cell lineage commitment Source: MGI
  • cell differentiation Source: MGI
  • cellular response to calcium ion Source: MGI
  • cellular response to drug Source: MGI
  • establishment of protein localization to membrane Source: MGI
  • face development Source: MGI
  • glucose transport Source: MGI
  • head morphogenesis Source: MGI
  • long-term synaptic potentiation Source: MGI
  • myeloid progenitor cell differentiation Source: MGI
  • negative regulation of apoptotic process Source: MGI
  • negative regulation of endothelial cell apoptotic process Source: MGI
  • negative regulation of fibroblast migration Source: MGI
  • negative regulation of neuron apoptotic process Source: MGI
  • negative regulation of synaptic vesicle exocytosis Source: MGI
  • positive regulation of axonogenesis Source: MGI
  • positive regulation of axon regeneration Source: MGI
  • positive regulation of ERK1 and ERK2 cascade Source: MGI
  • positive regulation of gene expression Source: MGI
  • positive regulation of peptidyl-serine phosphorylation Source: MGI
  • positive regulation of stress fiber assembly Source: MGI
  • positive regulation of substrate adhesion-dependent cell spreading Source: MGI
  • positive T cell selection Source: MGI
  • protein heterooligomerization Source: Ensembl
  • protein phosphorylation Source: BHF-UCL
  • regulation of axon regeneration Source: MGI
  • regulation of cell proliferation Source: MGI
  • regulation of T cell differentiation Source: MGI
  • response to cAMP Source: Ensembl
  • response to peptide hormone Source: Ensembl
  • somatic stem cell population maintenance Source: MGI
  • T cell receptor signaling pathway Source: MGI
  • thymus development Source: MGI
  • thyroid gland development Source: MGI
  • visual learning Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-1295596. Spry regulation of FGF signaling.
R-MMU-442742. CREB phosphorylation through the activation of Ras.
R-MMU-5673000. RAF activation.
R-MMU-5674135. MAP2K and MAPK activation.
R-MMU-5674499. Negative feedback regulation of MAPK pathway.
R-MMU-5675221. Negative regulation of MAPK pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase B-raf (EC:2.7.11.1)
Alternative name(s):
Proto-oncogene B-Raf
Gene namesi
Name:Braf
Synonyms:B-raf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:88190. Braf.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasm By similarity
  • Cell membrane By similarity

  • Note: Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes.By similarity

GO - Cellular componenti

  • cell body Source: MGI
  • cytoplasm Source: MGI
  • intracellular membrane-bounded organelle Source: MGI
  • mitochondrion Source: MGI
  • neuron projection Source: MGI
  • nucleus Source: UniProtKB-SubCell
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

Pathology & Biotechi

Involvement in diseasei

Participates in a chromosomal translocation that produces a Tif1a-BRAF (T18) oncogene originally isolated from a furfural-induced hepatoma.

Keywords - Diseasei

Proto-oncogene

Chemistry databases

ChEMBLiCHEMBL2331061.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000856662 – 804Serine/threonine-protein kinase B-rafAdd BLAST803

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei135PhosphoserineBy similarity1
Modified residuei316PhosphoserineCombined sources1
Modified residuei348PhosphoserineCombined sources1
Modified residuei356Phosphothreonine; by autocatalysisBy similarity1
Modified residuei431PhosphothreonineBy similarity1
Modified residuei434PhosphoserineBy similarity1
Modified residuei436PhosphothreonineBy similarity1
Modified residuei483PhosphoserineCombined sources1
Modified residuei484PhosphoserineCombined sources1
Cross-linki615Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei708Omega-N-methylarginine; by PRMT5By similarity1
Modified residuei766PhosphoserineBy similarity1
Modified residuei787PhosphoserineCombined sources1
Modified residuei790Phosphothreonine; by MAPK1By similarity1

Post-translational modificationi

Phosphorylation at Ser-348 by SGK1 inhibits its activity.By similarity
Methylation by PRMT5 decreases stability and kinase activity.1 Publication
Ubiquitinated by RNF149; which leads to proteasomal degradation. Polyubiquitinated at Lys-615 in response to EGF (By similarity).By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP28028.
MaxQBiP28028.
PaxDbiP28028.
PeptideAtlasiP28028.
PRIDEiP28028.

PTM databases

iPTMnetiP28028.
PhosphoSitePlusiP28028.

Expressioni

Gene expression databases

BgeeiENSMUSG00000002413.
CleanExiMM_BRAF.
ExpressionAtlasiP28028. baseline and differential.
GenevisibleiP28028. MM.

Interactioni

Subunit structurei

Monomer. Homodimer. Heterodimerizes with RAF1, and the heterodimer possesses a highly increased kinase activity compared to the respective homodimers or monomers. Heterodimerization is mitogen-regulated and enhanced by 14-3-3 proteins. MAPK1/ERK2 activation can induce a negative feedback that promotes the dissociation of the heterodimer by phosphorylating BRAF at Thr-790. Found in a complex with at least BRAF, HRAS, MAP2K1, MAPK3 and RGS14. Interacts with RIT1. Interacts (via N-terminus) with RGS14 (via RBD domains); the interaction mediates the formation of a ternary complex with RAF1, a ternary complex inhibited by GNAI1 (By similarity). Interacts with DGKH (By similarity). Interacts with PRMT5 (By similarity). Interacts with AKAP13, MAP2K1 and KSR1. Identified in a complex with AKAP13, KSR1 and MAP2K1 (PubMed:21102438). Interacts with FNIP1 and FNIP2 (By similarity).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Raf1Q99N572EBI-2584830,EBI-397757

GO - Molecular functioni

Protein-protein interaction databases

BioGridi225124. 3 interactors.
DIPiDIP-57050N.
IntActiP28028. 9 interactors.
MINTiMINT-1518176.
STRINGi10090.ENSMUSP00000002487.

Structurei

3D structure databases

ProteinModelPortaliP28028.
SMRiP28028.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini139 – 211RBDPROSITE-ProRule annotationAdd BLAST73
Domaini494 – 754Protein kinasePROSITE-ProRule annotationAdd BLAST261

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi6 – 30Poly-GlyAdd BLAST25
Compositional biasi465 – 469Poly-Ser5

Sequence similaritiesi

Contains 1 phorbol-ester/DAG-type zinc finger.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 RBD (Ras-binding) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0193. Eukaryota.
ENOG410Y4UP. LUCA.
GeneTreeiENSGT00760000118807.
HOGENOMiHOG000252972.
HOVERGENiHBG001886.
InParanoidiP28028.
KOiK04365.
OMAiCQQRERK.
OrthoDBiEOG091G09SB.
TreeFamiTF317006.

Family and domain databases

CDDicd00029. C1. 1 hit.
InterProiIPR020454. DAG/PE-bd.
IPR011009. Kinase-like_dom.
IPR002219. PE/DAG-bd.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR003116. RBD_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00130. C1_1. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
PF02196. RBD. 1 hit.
[Graphical view]
PRINTSiPR00008. DAGPEDOMAIN.
SMARTiSM00109. C1. 1 hit.
SM00455. RBD. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50898. RBD. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P28028-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAALSGGGGS SSGGGGGGGG GGGGGDGGGG AEQGQALFNG DMEPEAGAGA
60 70 80 90 100
AASSAADPAI PEEVWNIKQM IKLTQEHIEA LLDKFGGEHN PPSIYLEAYE
110 120 130 140 150
EYTSKLDALQ QREQQLLESL VFQTPTDASR NNPKSPQKPI VRVFLPNKQR
160 170 180 190 200
TVVPARCGVT VRDSLKKALM MRGLIPECCA VYRIQDGEKK PIGWDTDISW
210 220 230 240 250
LTGEELHVEV LENVPLTTHN FVRKTFFTLA FCDFCRKLLF QGFRCQTCGY
260 270 280 290 300
KFHQRCSTEV PLMCVNYDQL DLLFVSKFFE HHPVPQEEAS FPETALPSGS
310 320 330 340 350
SSAPPSDSTG PQILTSPSPS KSIPIPQPFR PADEDHRNQF GQRDRSSSAP
360 370 380 390 400
NVHINTIEPV NIDEKFPEVE LQDQRDLIRD QGFRGDGAPL NQLMRCLRKY
410 420 430 440 450
QSRTPSPLLH SVPSEIVFDF EPGPVFRGST TGLSATPPAS LPGSLTNVKA
460 470 480 490 500
LQKSPGPQRE RKSSSSSSSE DRSRMKTLGR RDSSDDWEIP DGQITVGQRI
510 520 530 540 550
GSGSFGTVYK GKWHGDVAVK MLNVTAPTPQ QLQAFKNEVG VLRKTRHVNI
560 570 580 590 600
LLFMGYSTKP QLAIVTQWCE GSSLYHHLHI IETKFEMIKL IDIARQTAQG
610 620 630 640 650
MDYLHAKSII HRDLKSNNIF LHEDLTVKIG DFGLATVKSR WSGSHQFEQL
660 670 680 690 700
SGSILWMAPE VIRMQDKNPY SFQSDVYAFG IVLYELMTGQ LPYSNINNRD
710 720 730 740 750
QIIFMVGRGY LSPDLSKVRS NCPKAMKRLM AECLKKKRDE RPLFPQILAS
760 770 780 790 800
IELLARSLPK IHRSASEPSL NRAGFQTEDF SLYACASPKT PIQAGGYGEF

AAFK
Length:804
Mass (Da):88,780
Last modified:July 27, 2011 - v3
Checksum:i4C6CC16A2C660A17
GO
Isoform 2 (identifier: P28028-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-474: Missing.
     799-804: EFAAFK → GFPVH

Note: No experimental confirmation available.
Show »
Length:329
Mass (Da):37,118
Checksum:i93913670A27EAB2C
GO

Sequence cautioni

The sequence AAA37320 differs from that shown. Chimeric cDNA.Curated
The sequence AAA37320 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti10 – 11SS → RR in BAE24384 (PubMed:16141072).Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0221691 – 474Missing in isoform 2. 1 PublicationAdd BLAST474
Alternative sequenceiVSP_022170799 – 804EFAAFK → GFPVH in isoform 2. 1 Publication6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK140431 mRNA. Translation: BAE24384.1.
AC122345 Genomic DNA. No translation available.
AC163109 Genomic DNA. No translation available.
M64429 mRNA. Translation: AAA37320.1. Sequence problems.
CCDSiCCDS39463.1. [P28028-1]
PIRiA40951. TVMSBF.
RefSeqiNP_647455.3. NM_139294.5. [P28028-1]
UniGeneiMm.245513.
Mm.489691.

Genome annotation databases

EnsembliENSMUST00000002487; ENSMUSP00000002487; ENSMUSG00000002413. [P28028-1]
GeneIDi109880.
KEGGimmu:109880.
UCSCiuc009bme.2. mouse. [P28028-1]
uc009bmf.1. mouse. [P28028-2]

Keywords - Coding sequence diversityi

Alternative splicing, Chromosomal rearrangement

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK140431 mRNA. Translation: BAE24384.1.
AC122345 Genomic DNA. No translation available.
AC163109 Genomic DNA. No translation available.
M64429 mRNA. Translation: AAA37320.1. Sequence problems.
CCDSiCCDS39463.1. [P28028-1]
PIRiA40951. TVMSBF.
RefSeqiNP_647455.3. NM_139294.5. [P28028-1]
UniGeneiMm.245513.
Mm.489691.

3D structure databases

ProteinModelPortaliP28028.
SMRiP28028.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi225124. 3 interactors.
DIPiDIP-57050N.
IntActiP28028. 9 interactors.
MINTiMINT-1518176.
STRINGi10090.ENSMUSP00000002487.

Chemistry databases

ChEMBLiCHEMBL2331061.

PTM databases

iPTMnetiP28028.
PhosphoSitePlusiP28028.

Proteomic databases

EPDiP28028.
MaxQBiP28028.
PaxDbiP28028.
PeptideAtlasiP28028.
PRIDEiP28028.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000002487; ENSMUSP00000002487; ENSMUSG00000002413. [P28028-1]
GeneIDi109880.
KEGGimmu:109880.
UCSCiuc009bme.2. mouse. [P28028-1]
uc009bmf.1. mouse. [P28028-2]

Organism-specific databases

CTDi673.
MGIiMGI:88190. Braf.

Phylogenomic databases

eggNOGiKOG0193. Eukaryota.
ENOG410Y4UP. LUCA.
GeneTreeiENSGT00760000118807.
HOGENOMiHOG000252972.
HOVERGENiHBG001886.
InParanoidiP28028.
KOiK04365.
OMAiCQQRERK.
OrthoDBiEOG091G09SB.
TreeFamiTF317006.

Enzyme and pathway databases

ReactomeiR-MMU-1295596. Spry regulation of FGF signaling.
R-MMU-442742. CREB phosphorylation through the activation of Ras.
R-MMU-5673000. RAF activation.
R-MMU-5674135. MAP2K and MAPK activation.
R-MMU-5674499. Negative feedback regulation of MAPK pathway.
R-MMU-5675221. Negative regulation of MAPK pathway.

Miscellaneous databases

ChiTaRSiBraf. mouse.
PROiP28028.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000002413.
CleanExiMM_BRAF.
ExpressionAtlasiP28028. baseline and differential.
GenevisibleiP28028. MM.

Family and domain databases

CDDicd00029. C1. 1 hit.
InterProiIPR020454. DAG/PE-bd.
IPR011009. Kinase-like_dom.
IPR002219. PE/DAG-bd.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR003116. RBD_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00130. C1_1. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
PF02196. RBD. 1 hit.
[Graphical view]
PRINTSiPR00008. DAGPEDOMAIN.
SMARTiSM00109. C1. 1 hit.
SM00455. RBD. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50898. RBD. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBRAF_MOUSE
AccessioniPrimary (citable) accession number: P28028
Secondary accession number(s): E9QNG9, Q3USE9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 169 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.