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P28026

- WNT8_XENLA

UniProt

P28026 - WNT8_XENLA

Protein

Protein Wnt-8

Gene

wnt8

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 2 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Ligand for members of the frizzled family of seven transmembrane receptors. Plays a role in ventral mesodermal patterning during embryogenesis. Mimics Nieuwkoop center activity. Causes dorsal mesodermal differentiation of animal cap ectoderm when coexpressed with noggin and nuclear, sequence-specific DNA-binding protein xBra. None of these molecules causes dorsal mesoderm formation when expressed alone.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei39 – 402Cleavage; by tiki1 and tiki2
    Sitei42 – 432Cleavage; by tiki1 and tiki2

    GO - Molecular functioni

    1. protein binding Source: UniProtKB

    GO - Biological processi

    1. canonical Wnt signaling pathway Source: BHF-UCL
    2. canonical Wnt signaling pathway involved in neural crest cell differentiation Source: BHF-UCL
    3. embryonic axis specification Source: BHF-UCL
    4. negative regulation of cardiac cell fate specification Source: BHF-UCL
    5. neural crest cell fate commitment Source: BHF-UCL
    6. positive regulation of sequence-specific DNA binding transcription factor activity Source: BHF-UCL
    7. regulation of transcription involved in anterior/posterior axis specification Source: BHF-UCL
    8. Spemann organizer formation Source: BHF-UCL
    9. Wnt signaling pathway Source: BHF-UCL

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Wnt signaling pathway

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein Wnt-8
    Short name:
    XWnt-8
    Gene namesi
    Name:wnt8
    OrganismiXenopus laevis (African clawed frog)
    Taxonomic identifieri8355 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

    Organism-specific databases

    XenbaseiXB-GENE-866281. wnt8a.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: Reactome
    2. proteinaceous extracellular matrix Source: BHF-UCL

    Keywords - Cellular componenti

    Extracellular matrix, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222Sequence AnalysisAdd
    BLAST
    Chaini23 – 358336Protein Wnt-8PRO_0000041447Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi55 ↔ 661 Publication
    Glycosylationi104 – 1041N-linked (GlcNAc...)1 Publication
    Disulfide bondi105 ↔ 1131 Publication
    Disulfide bondi115 ↔ 1331 Publication
    Disulfide bondi181 ↔ 1951 Publication
    Disulfide bondi183 ↔ 1901 Publication
    Lipidationi187 – 1871O-palmitoyl serine1 Publication
    Disulfide bondi260 ↔ 2981 Publication
    Glycosylationi263 – 2631N-linked (GlcNAc...)1 Publication
    Disulfide bondi276 ↔ 2911 Publication
    Glycosylationi282 – 2821N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi295 ↔ 3371 Publication
    Disulfide bondi313 ↔ 3281 Publication
    Disulfide bondi315 ↔ 3251 Publication
    Disulfide bondi320 ↔ 3211 Publication

    Post-translational modificationi

    Palmitoylation at Ser-187 is required for efficient binding to frizzled receptors. Palmitoylation is necessary for proper trafficking to cell surface By similarity.By similarity
    Proteolytic processing by tiki1 and tiki2 promotes oxidation and formation of large disulfide-bond oligomers, leading to inactivation of wnt8.1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

    Expressioni

    Developmental stagei

    Mid-blastula, decline by tailbud.

    Interactioni

    Subunit structurei

    Homooligomer; disulfide-linked, leading to inactivation. Interacts with the long chain of cer1.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ptk7Q6PA072EBI-6257743,EBI-7036323

    Protein-protein interaction databases

    BioGridi99305. 1 interaction.
    IntActiP28026. 2 interactions.
    MINTiMINT-8291189.

    Structurei

    Secondary structure

    1
    358
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi34 – 5825
    Turni59 – 613
    Beta strandi62 – 643
    Helixi72 – 754
    Helixi84 – 10623
    Turni107 – 1093
    Turni118 – 1214
    Beta strandi122 – 1254
    Beta strandi128 – 1303
    Helixi137 – 15115
    Beta strandi154 – 1563
    Helixi157 – 17519
    Beta strandi178 – 1836
    Helixi186 – 1883
    Beta strandi192 – 1987
    Helixi202 – 21413
    Helixi236 – 2438
    Beta strandi247 – 2493
    Beta strandi261 – 2633
    Turni264 – 2674
    Helixi285 – 2884
    Helixi290 – 2945
    Helixi296 – 2983
    Beta strandi301 – 31212
    Beta strandi319 – 3213
    Beta strandi327 – 33812

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4F0AX-ray3.25B23-338[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the Wnt family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    HOVERGENiHBG001595.
    KOiK00714.

    Family and domain databases

    InterProiIPR005817. Wnt.
    IPR013301. Wnt8.
    IPR018161. Wnt_CS.
    [Graphical view]
    PANTHERiPTHR12027. PTHR12027. 1 hit.
    PTHR12027:SF92. PTHR12027:SF92. 1 hit.
    PfamiPF00110. wnt. 1 hit.
    [Graphical view]
    PRINTSiPR01892. WNT8PROTEIN.
    PR01349. WNTPROTEIN.
    SMARTiSM00097. WNT1. 1 hit.
    [Graphical view]
    PROSITEiPS00246. WNT1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P28026-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQNTTLFILA TLLIFCPFFT ASAWSVNNFL MTGPKAYLTY SASVAVGAQN    50
    GIEECKYQFA WERWNCPEST LQLATHNGLR SATRETSFVH AISSAGVMYT 100
    LTRNCSMGDF DNCGCDDSRN GRIGGRGWVW GGCSDNAEFG ERISKLFVDG 150
    LETGQDARAL MNLHNNEAGR LAVKETMKRT CKCHGISGSC SIQTCWLQLA 200
    EFRDIGNHLK IKHDQALKLE MDKRKMRSGN SADNRGAIAD AFSSVAGSEL 250
    IFLEDSPDYC LKNISLGLQG TEGRECLQSG KNLSQWERRS CKRLCTDCGL 300
    RVEEKKTEII SSCNCKFHWC CTVKCEQCKQ VVIKHFCARR ERDSNMLNTK 350
    RKNRGHRR 358
    Length:358
    Mass (Da):40,176
    Last modified:October 1, 1996 - v2
    Checksum:i8BC8B9E20016E504
    GO

    Sequence cautioni

    The sequence AAA50012.1 differs from that shown. Reason:

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti243 – 2431S → T in AAA50012. (PubMed:1991549)Curated
    Sequence conflicti292 – 2921K → R in AAA50012. (PubMed:1991549)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X57234 mRNA. Translation: CAA40510.1.
    M55058 mRNA. Translation: AAA50012.1. Sequence problems.
    PIRiS18771.
    RefSeqiNP_001081637.1. NM_001088168.1.
    UniGeneiXl.49.

    Genome annotation databases

    GeneIDi397970.
    KEGGixla:397970.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X57234 mRNA. Translation: CAA40510.1 .
    M55058 mRNA. Translation: AAA50012.1 . Sequence problems.
    PIRi S18771.
    RefSeqi NP_001081637.1. NM_001088168.1.
    UniGenei Xl.49.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4F0A X-ray 3.25 B 23-338 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 99305. 1 interaction.
    IntActi P28026. 2 interactions.
    MINTi MINT-8291189.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 397970.
    KEGGi xla:397970.

    Organism-specific databases

    CTDi 7478.
    Xenbasei XB-GENE-866281. wnt8a.

    Phylogenomic databases

    HOVERGENi HBG001595.
    KOi K00714.

    Family and domain databases

    InterProi IPR005817. Wnt.
    IPR013301. Wnt8.
    IPR018161. Wnt_CS.
    [Graphical view ]
    PANTHERi PTHR12027. PTHR12027. 1 hit.
    PTHR12027:SF92. PTHR12027:SF92. 1 hit.
    Pfami PF00110. wnt. 1 hit.
    [Graphical view ]
    PRINTSi PR01892. WNT8PROTEIN.
    PR01349. WNTPROTEIN.
    SMARTi SM00097. WNT1. 1 hit.
    [Graphical view ]
    PROSITEi PS00246. WNT1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Xwnt-8, a Xenopus Wnt-1/int-1-related gene responsive to mesoderm-inducing growth factors, may play a role in ventral mesodermal patterning during embryogenesis."
      Christian J.L., McMahon J.A., McMahon A.P., Moon R.T.
      Development 111:1045-1055(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Neurula.
    2. "Xwnt-8b: a maternally expressed Xenopus Wnt gene with a potential role in establishing the dorsoventral axis."
      Cui Y., Brown J.D., Moon R.T., Christian J.L.
      Development 121:2177-2186(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: SEQUENCE REVISION TO C-TERMINUS.
    3. "Isolation of cDNAs partially encoding four Xenopus Wnt-1/int-1-related proteins and characterization of their transient expression during embryonic development."
      Christian J.L., Gavin B.J., McMahon A.P., Moon R.T.
      Dev. Biol. 143:230-234(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 181-321.
      Tissue: Neurula.
    4. "Specification of mesodermal pattern in Xenopus laevis by interactions between Brachyury, noggin and Xwnt-8."
      Cunliffe V., Smith J.C.
      EMBO J. 13:349-359(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "The head inducer Cerberus is a multifunctional antagonist of Nodal, BMP and Wnt signals."
      Piccolo S., Agius E., Leyns L., Bhattacharyya S., Grunz H., Bouwmeester T., De Robertis E.M.
      Nature 397:707-710(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CER1.
    6. "Tiki1 is required for head formation via Wnt cleavage-oxidation and inactivation."
      Zhang X., Abreu J.G., Yokota C., Macdonald B.T., Singh S., Coburn K.L., Cheong S.M., Zhang M.M., Ye Q.Z., Hang H.C., Steen H., He X.
      Cell 149:1565-1577(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEOLYTIC PROCESSING BY TIKI1 AND TIKI2, SUBUNIT.
    7. "Structural basis of Wnt recognition by Frizzled."
      Janda C.Y., Waghray D., Levin A.M., Thomas C., Garcia K.C.
      Science 337:59-64(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.25 ANGSTROMS) OF 23-338 IN COMPLEX WITH MOUSE FZD8, GLYCOSYLATION AT ASN-104 AND ASN-263, PALMITOYLATION AT SER-187, DISULFIDE BONDS.

    Entry informationi

    Entry nameiWNT8_XENLA
    AccessioniPrimary (citable) accession number: P28026
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 90 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3