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P28026

- WNT8_XENLA

UniProt

P28026 - WNT8_XENLA

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Protein
Protein Wnt-8
Gene
wnt8
Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Ligand for members of the frizzled family of seven transmembrane receptors. Plays a role in ventral mesodermal patterning during embryogenesis. Mimics Nieuwkoop center activity. Causes dorsal mesodermal differentiation of animal cap ectoderm when coexpressed with noggin and nuclear, sequence-specific DNA-binding protein xBra. None of these molecules causes dorsal mesoderm formation when expressed alone.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei39 – 402Cleavage; by tiki1 and tiki2
Sitei42 – 432Cleavage; by tiki1 and tiki2

GO - Molecular functioni

  1. protein binding Source: UniProtKB

GO - Biological processi

  1. Spemann organizer formation Source: BHF-UCL
  2. Wnt signaling pathway Source: BHF-UCL
  3. canonical Wnt signaling pathway Source: BHF-UCL
  4. canonical Wnt signaling pathway involved in neural crest cell differentiation Source: BHF-UCL
  5. embryonic axis specification Source: BHF-UCL
  6. negative regulation of cardiac cell fate specification Source: BHF-UCL
  7. neural crest cell fate commitment Source: BHF-UCL
  8. positive regulation of sequence-specific DNA binding transcription factor activity Source: BHF-UCL
  9. regulation of transcription involved in anterior/posterior axis specification Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Wnt signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Wnt-8
Short name:
XWnt-8
Gene namesi
Name:wnt8
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-866281. wnt8a.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: Reactome
  2. proteinaceous extracellular matrix Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222 Reviewed prediction
Add
BLAST
Chaini23 – 358336Protein Wnt-8
PRO_0000041447Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi55 ↔ 661 Publication
Glycosylationi104 – 1041N-linked (GlcNAc...)1 Publication
Disulfide bondi105 ↔ 1131 Publication
Disulfide bondi115 ↔ 1331 Publication
Disulfide bondi181 ↔ 1951 Publication
Disulfide bondi183 ↔ 1901 Publication
Lipidationi187 – 1871O-palmitoyl serine1 Publication
Disulfide bondi260 ↔ 2981 Publication
Glycosylationi263 – 2631N-linked (GlcNAc...)1 Publication
Disulfide bondi276 ↔ 2911 Publication
Glycosylationi282 – 2821N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi295 ↔ 3371 Publication
Disulfide bondi313 ↔ 3281 Publication
Disulfide bondi315 ↔ 3251 Publication
Disulfide bondi320 ↔ 3211 Publication

Post-translational modificationi

Palmitoylation at Ser-187 is required for efficient binding to frizzled receptors. Palmitoylation is necessary for proper trafficking to cell surface By similarity.1 Publication
Proteolytic processing by tiki1 and tiki2 promotes oxidation and formation of large disulfide-bond oligomers, leading to inactivation of wnt8.

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Expressioni

Developmental stagei

Mid-blastula, decline by tailbud.

Interactioni

Subunit structurei

Homooligomer; disulfide-linked, leading to inactivation. Interacts with the long chain of cer1.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ptk7Q6PA072EBI-6257743,EBI-7036323

Protein-protein interaction databases

BioGridi99305. 1 interaction.
IntActiP28026. 2 interactions.
MINTiMINT-8291189.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi34 – 5825
Turni59 – 613
Beta strandi62 – 643
Helixi72 – 754
Helixi84 – 10623
Turni107 – 1093
Turni118 – 1214
Beta strandi122 – 1254
Beta strandi128 – 1303
Helixi137 – 15115
Beta strandi154 – 1563
Helixi157 – 17519
Beta strandi178 – 1836
Helixi186 – 1883
Beta strandi192 – 1987
Helixi202 – 21413
Helixi236 – 2438
Beta strandi247 – 2493
Beta strandi261 – 2633
Turni264 – 2674
Helixi285 – 2884
Helixi290 – 2945
Helixi296 – 2983
Beta strandi301 – 31212
Beta strandi319 – 3213
Beta strandi327 – 33812

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4F0AX-ray3.25B23-338[»]

Family & Domainsi

Sequence similaritiesi

Belongs to the Wnt family.

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG001595.
KOiK00714.

Family and domain databases

InterProiIPR005817. Wnt.
IPR013301. Wnt8.
IPR018161. Wnt_CS.
[Graphical view]
PANTHERiPTHR12027. PTHR12027. 1 hit.
PTHR12027:SF92. PTHR12027:SF92. 1 hit.
PfamiPF00110. wnt. 1 hit.
[Graphical view]
PRINTSiPR01892. WNT8PROTEIN.
PR01349. WNTPROTEIN.
SMARTiSM00097. WNT1. 1 hit.
[Graphical view]
PROSITEiPS00246. WNT1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28026-1 [UniParc]FASTAAdd to Basket

« Hide

MQNTTLFILA TLLIFCPFFT ASAWSVNNFL MTGPKAYLTY SASVAVGAQN    50
GIEECKYQFA WERWNCPEST LQLATHNGLR SATRETSFVH AISSAGVMYT 100
LTRNCSMGDF DNCGCDDSRN GRIGGRGWVW GGCSDNAEFG ERISKLFVDG 150
LETGQDARAL MNLHNNEAGR LAVKETMKRT CKCHGISGSC SIQTCWLQLA 200
EFRDIGNHLK IKHDQALKLE MDKRKMRSGN SADNRGAIAD AFSSVAGSEL 250
IFLEDSPDYC LKNISLGLQG TEGRECLQSG KNLSQWERRS CKRLCTDCGL 300
RVEEKKTEII SSCNCKFHWC CTVKCEQCKQ VVIKHFCARR ERDSNMLNTK 350
RKNRGHRR 358
Length:358
Mass (Da):40,176
Last modified:October 1, 1996 - v2
Checksum:i8BC8B9E20016E504
GO

Sequence cautioni

The sequence AAA50012.1 differs from that shown. Reason:

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti243 – 2431S → T in AAA50012. 1 Publication
Sequence conflicti292 – 2921K → R in AAA50012. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X57234 mRNA. Translation: CAA40510.1.
M55058 mRNA. Translation: AAA50012.1. Sequence problems.
PIRiS18771.
RefSeqiNP_001081637.1. NM_001088168.1.
UniGeneiXl.49.

Genome annotation databases

GeneIDi397970.
KEGGixla:397970.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X57234 mRNA. Translation: CAA40510.1 .
M55058 mRNA. Translation: AAA50012.1 . Sequence problems.
PIRi S18771.
RefSeqi NP_001081637.1. NM_001088168.1.
UniGenei Xl.49.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4F0A X-ray 3.25 B 23-338 [» ]
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 99305. 1 interaction.
IntActi P28026. 2 interactions.
MINTi MINT-8291189.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 397970.
KEGGi xla:397970.

Organism-specific databases

CTDi 7478.
Xenbasei XB-GENE-866281. wnt8a.

Phylogenomic databases

HOVERGENi HBG001595.
KOi K00714.

Family and domain databases

InterProi IPR005817. Wnt.
IPR013301. Wnt8.
IPR018161. Wnt_CS.
[Graphical view ]
PANTHERi PTHR12027. PTHR12027. 1 hit.
PTHR12027:SF92. PTHR12027:SF92. 1 hit.
Pfami PF00110. wnt. 1 hit.
[Graphical view ]
PRINTSi PR01892. WNT8PROTEIN.
PR01349. WNTPROTEIN.
SMARTi SM00097. WNT1. 1 hit.
[Graphical view ]
PROSITEi PS00246. WNT1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Xwnt-8, a Xenopus Wnt-1/int-1-related gene responsive to mesoderm-inducing growth factors, may play a role in ventral mesodermal patterning during embryogenesis."
    Christian J.L., McMahon J.A., McMahon A.P., Moon R.T.
    Development 111:1045-1055(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Neurula.
  2. "Xwnt-8b: a maternally expressed Xenopus Wnt gene with a potential role in establishing the dorsoventral axis."
    Cui Y., Brown J.D., Moon R.T., Christian J.L.
    Development 121:2177-2186(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION TO C-TERMINUS.
  3. "Isolation of cDNAs partially encoding four Xenopus Wnt-1/int-1-related proteins and characterization of their transient expression during embryonic development."
    Christian J.L., Gavin B.J., McMahon A.P., Moon R.T.
    Dev. Biol. 143:230-234(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 181-321.
    Tissue: Neurula.
  4. "Specification of mesodermal pattern in Xenopus laevis by interactions between Brachyury, noggin and Xwnt-8."
    Cunliffe V., Smith J.C.
    EMBO J. 13:349-359(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "The head inducer Cerberus is a multifunctional antagonist of Nodal, BMP and Wnt signals."
    Piccolo S., Agius E., Leyns L., Bhattacharyya S., Grunz H., Bouwmeester T., De Robertis E.M.
    Nature 397:707-710(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CER1.
  6. "Tiki1 is required for head formation via Wnt cleavage-oxidation and inactivation."
    Zhang X., Abreu J.G., Yokota C., Macdonald B.T., Singh S., Coburn K.L., Cheong S.M., Zhang M.M., Ye Q.Z., Hang H.C., Steen H., He X.
    Cell 149:1565-1577(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEOLYTIC PROCESSING BY TIKI1 AND TIKI2, SUBUNIT.
  7. "Structural basis of Wnt recognition by Frizzled."
    Janda C.Y., Waghray D., Levin A.M., Thomas C., Garcia K.C.
    Science 337:59-64(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.25 ANGSTROMS) OF 23-338 IN COMPLEX WITH MOUSE FZD8, GLYCOSYLATION AT ASN-104 AND ASN-263, PALMITOYLATION AT SER-187, DISULFIDE BONDS.

Entry informationi

Entry nameiWNT8_XENLA
AccessioniPrimary (citable) accession number: P28026
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: October 1, 1996
Last modified: September 3, 2014
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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