Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein Wnt-8

Gene

wnt8

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ligand for members of the frizzled family of seven transmembrane receptors. Plays a role in ventral mesodermal patterning during embryogenesis. Mimics Nieuwkoop center activity. Causes dorsal mesodermal differentiation of animal cap ectoderm when coexpressed with noggin and nuclear, sequence-specific DNA-binding protein xBra. None of these molecules causes dorsal mesoderm formation when expressed alone.1 Publication

GO - Molecular functioni

  • frizzled binding Source: ParkinsonsUK-UCL
  • protease binding Source: ParkinsonsUK-UCL

GO - Biological processi

  • canonical Wnt signaling pathway Source: BHF-UCL
  • canonical Wnt signaling pathway involved in neural crest cell differentiation Source: BHF-UCL
  • embryonic axis specification Source: BHF-UCL
  • negative regulation of cardiac cell fate specification Source: BHF-UCL
  • neural crest cell fate commitment Source: BHF-UCL
  • positive regulation of sequence-specific DNA binding transcription factor activity Source: BHF-UCL
  • regulation of transcription involved in anterior/posterior axis specification Source: BHF-UCL
  • Spemann organizer formation Source: BHF-UCL
  • Wnt signaling pathway Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Wnt signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Wnt-8
Short name:
XWnt-8
Gene namesi
Name:wnt8
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-866281. wnt8a.

Subcellular locationi

GO - Cellular componenti

  • extracellular region Source: Reactome
  • proteinaceous extracellular matrix Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000004144723 – 358Protein Wnt-8Add BLAST336

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi55 ↔ 661 Publication
Glycosylationi104N-linked (GlcNAc...)1 Publication1
Disulfide bondi105 ↔ 1131 Publication
Disulfide bondi115 ↔ 1331 Publication
Disulfide bondi181 ↔ 1951 Publication
Disulfide bondi183 ↔ 1901 Publication
Lipidationi187O-palmitoleyl serine1 Publication1
Disulfide bondi260 ↔ 2981 Publication
Glycosylationi263N-linked (GlcNAc...)1 Publication1
Disulfide bondi276 ↔ 2911 Publication
Glycosylationi282N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi295 ↔ 3371 Publication
Disulfide bondi313 ↔ 3281 Publication
Disulfide bondi315 ↔ 3251 Publication
Disulfide bondi320 ↔ 3211 Publication

Post-translational modificationi

Palmitoleylation is required for efficient binding to frizzled receptors (PubMed:22653731). Depalmitoleylation leads to Wnt signaling pathway inhibition (By similarity).By similarity1 Publication
Proteolytic processing by tiki1 and tiki2 promotes oxidation and formation of large disulfide-bond oligomers, leading to inactivation of wnt8.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei39 – 40Cleavage; by tiki1 and tiki21 Publication2
Sitei42 – 43Cleavage; by tiki1 and tiki21 Publication2

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein

Expressioni

Developmental stagei

Mid-blastula, decline by tailbud.

Interactioni

Subunit structurei

Homooligomer; disulfide-linked, leading to inactivation. Interacts with the long chain of cer1.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ptk7Q6PA072EBI-6257743,EBI-7036323

GO - Molecular functioni

  • frizzled binding Source: ParkinsonsUK-UCL
  • protease binding Source: ParkinsonsUK-UCL

Protein-protein interaction databases

BioGridi99305. 1 interactor.
IntActiP28026. 2 interactors.
MINTiMINT-8291189.

Structurei

Secondary structure

1358
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi34 – 58Combined sources25
Turni59 – 61Combined sources3
Beta strandi62 – 64Combined sources3
Helixi72 – 75Combined sources4
Helixi84 – 106Combined sources23
Turni107 – 109Combined sources3
Turni118 – 121Combined sources4
Beta strandi122 – 125Combined sources4
Beta strandi128 – 130Combined sources3
Helixi137 – 151Combined sources15
Beta strandi154 – 156Combined sources3
Helixi157 – 175Combined sources19
Beta strandi178 – 183Combined sources6
Helixi186 – 188Combined sources3
Beta strandi192 – 198Combined sources7
Helixi202 – 214Combined sources13
Helixi236 – 243Combined sources8
Beta strandi247 – 249Combined sources3
Beta strandi261 – 263Combined sources3
Turni264 – 267Combined sources4
Helixi285 – 288Combined sources4
Helixi290 – 294Combined sources5
Helixi296 – 298Combined sources3
Beta strandi301 – 312Combined sources12
Beta strandi319 – 321Combined sources3
Beta strandi327 – 338Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4F0AX-ray3.25B23-338[»]
SMRiP28026.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Wnt family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG001595.
KOiK00714.

Family and domain databases

InterProiIPR005817. Wnt.
IPR013301. Wnt8.
IPR018161. Wnt_CS.
[Graphical view]
PANTHERiPTHR12027. PTHR12027. 1 hit.
PTHR12027:SF92. PTHR12027:SF92. 1 hit.
PfamiPF00110. wnt. 1 hit.
[Graphical view]
PRINTSiPR01892. WNT8PROTEIN.
PR01349. WNTPROTEIN.
SMARTiSM00097. WNT1. 1 hit.
[Graphical view]
PROSITEiPS00246. WNT1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28026-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQNTTLFILA TLLIFCPFFT ASAWSVNNFL MTGPKAYLTY SASVAVGAQN
60 70 80 90 100
GIEECKYQFA WERWNCPEST LQLATHNGLR SATRETSFVH AISSAGVMYT
110 120 130 140 150
LTRNCSMGDF DNCGCDDSRN GRIGGRGWVW GGCSDNAEFG ERISKLFVDG
160 170 180 190 200
LETGQDARAL MNLHNNEAGR LAVKETMKRT CKCHGISGSC SIQTCWLQLA
210 220 230 240 250
EFRDIGNHLK IKHDQALKLE MDKRKMRSGN SADNRGAIAD AFSSVAGSEL
260 270 280 290 300
IFLEDSPDYC LKNISLGLQG TEGRECLQSG KNLSQWERRS CKRLCTDCGL
310 320 330 340 350
RVEEKKTEII SSCNCKFHWC CTVKCEQCKQ VVIKHFCARR ERDSNMLNTK

RKNRGHRR
Length:358
Mass (Da):40,176
Last modified:October 1, 1996 - v2
Checksum:i8BC8B9E20016E504
GO

Sequence cautioni

The sequence AAA50012 differs from that shown.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti243S → T in AAA50012 (PubMed:1991549).Curated1
Sequence conflicti292K → R in AAA50012 (PubMed:1991549).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57234 mRNA. Translation: CAA40510.1.
M55058 mRNA. Translation: AAA50012.1. Sequence problems.
PIRiS18771.
RefSeqiNP_001081637.1. NM_001088168.1.
UniGeneiXl.49.

Genome annotation databases

GeneIDi397970.
KEGGixla:397970.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57234 mRNA. Translation: CAA40510.1.
M55058 mRNA. Translation: AAA50012.1. Sequence problems.
PIRiS18771.
RefSeqiNP_001081637.1. NM_001088168.1.
UniGeneiXl.49.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4F0AX-ray3.25B23-338[»]
SMRiP28026.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi99305. 1 interactor.
IntActiP28026. 2 interactors.
MINTiMINT-8291189.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi397970.
KEGGixla:397970.

Organism-specific databases

CTDi7478.
XenbaseiXB-GENE-866281. wnt8a.

Phylogenomic databases

HOVERGENiHBG001595.
KOiK00714.

Family and domain databases

InterProiIPR005817. Wnt.
IPR013301. Wnt8.
IPR018161. Wnt_CS.
[Graphical view]
PANTHERiPTHR12027. PTHR12027. 1 hit.
PTHR12027:SF92. PTHR12027:SF92. 1 hit.
PfamiPF00110. wnt. 1 hit.
[Graphical view]
PRINTSiPR01892. WNT8PROTEIN.
PR01349. WNTPROTEIN.
SMARTiSM00097. WNT1. 1 hit.
[Graphical view]
PROSITEiPS00246. WNT1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiWNT8_XENLA
AccessioniPrimary (citable) accession number: P28026
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.