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P28019 (DYR_AEDAL) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dihydrofolate reductase

EC=1.5.1.3
Gene names
Name:DHFR
OrganismAedes albopictus (Asian tiger mosquito) (Stegomyia albopicta)
Taxonomic identifier7160 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeCulicinaeAediniAedesStegomyia

Protein attributes

Sequence length186 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis By similarity.

Catalytic activity

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.

Pathway

Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.

Sequence similarities

Belongs to the dihydrofolate reductase family.

Contains 1 DHFR (dihydrofolate reductase) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 186186Dihydrofolate reductase
PRO_0000186369

Regions

Domain3 – 183181DHFR
Nucleotide binding15 – 217NADP By similarity
Nucleotide binding53 – 553NADP By similarity
Nucleotide binding75 – 773NADP By similarity
Nucleotide binding116 – 1238NADP By similarity
Region29 – 346Substrate binding By similarity

Sites

Binding site91NADP; via amide nitrogen and carbonyl oxygen By similarity
Binding site691Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
P28019 [UniParc].

Last modified August 1, 1992. Version 1.
Checksum: AFCD7F469779D83C

FASTA18621,447
        10         20         30         40         50         60 
MKKFSLIVAV CANGGIGIKG DLPWRLRQEL KYFSRMTKKI QDSGKRNAII MGRKTYFGVP 

        70         80         90        100        110        120 
ESKRPLPERL NIILTRDPSA NAYPSEVMVC TSMQEALKKL DEAPLVNEIE NVWIVGGNAV 

       130        140        150        160        170        180 
YKEAMQSDRC HRIYLTEIKE TFECDAFFPE ITSDFQLVKN DDDVPEDIQE ENGIQYQYRI 


YEKVPK 

« Hide

References

[1]"An amplified insect dihydrofolate reductase gene contains a single intron."
Shotkoski F.A., Fallon A.M.
Eur. J. Biochem. 201:157-160(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X60192 Genomic DNA. Translation: CAA42748.1.
PIRS17984.

3D structure databases

ProteinModelPortalP28019.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00077; UER00158.

Family and domain databases

Gene3D3.40.430.10. 1 hit.
InterProIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamPF00186. DHFR_1. 1 hit.
[Graphical view]
PIRSFPIRSF000194. DHFR. 1 hit.
PRINTSPR00070. DHFR.
SUPFAMSSF53597. SSF53597. 1 hit.
PROSITEPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDYR_AEDAL
AccessionPrimary (citable) accession number: P28019
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: October 16, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways