P28019 (DYR_AEDAL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydrofolate reductase
EC=1.5.1.3

Gene names

Name:

DHFR

Organism

Aedes albopictus (Asian tiger mosquito) (Culex albopictus)

Taxonomic identifier

7160 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Nematocera › Culicoidea › Culicidae › Culicinae › Aedini › Aedes › Stegomyia

Protein attributes

Sequence length	186 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis By similarity.
Catalytic activity	5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.
Pathway	Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.
Sequence similarities	Belongs to the dihydrofolate reductase family. Contains 1 DHFR (dihydrofolate reductase) domain.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	glycine biosynthetic process Inferred from electronic annotation. Source: InterPro nucleotide biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro dihydrofolate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 186	186	Dihydrofolate reductase		PRO_0000186369
Regions
	Domain	3 – 183	181	DHFR
	Nucleotide binding	15 – 21	7	NADP By similarity
	Nucleotide binding	53 – 55	3	NADP By similarity
	Nucleotide binding	75 – 77	3	NADP By similarity
	Nucleotide binding	116 – 123	8	NADP By similarity
	Region	29 – 34	6	Substrate binding By similarity
Sites
	Binding site	9	1	NADP; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	69	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P28019 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: AFCD7F469779D83C	FASTA	186	21,447
	10 20 30 40 50 60 MKKFSLIVAV CANGGIGIKG DLPWRLRQEL KYFSRMTKKI QDSGKRNAII MGRKTYFGVP 70 80 90 100 110 120 ESKRPLPERL NIILTRDPSA NAYPSEVMVC TSMQEALKKL DEAPLVNEIE NVWIVGGNAV 130 140 150 160 170 180 YKEAMQSDRC HRIYLTEIKE TFECDAFFPE ITSDFQLVKN DDDVPEDIQE ENGIQYQYRI YEKVPK

References

[1]	"An amplified insect dihydrofolate reductase gene contains a single intron." Shotkoski F.A., Fallon A.M. Eur. J. Biochem. 201:157-160(1991) [PubMed: 1915358] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X60192 Genomic DNA. Translation: CAA42748.1.
PIR	S17984.
3D structure databases
ProteinModelPortal	P28019.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR012259. DHFR. IPR024072. DHFR-like_dom. IPR017925. DHFR_CS. IPR001796. DHFR_dom. [Graphical view]
Gene3D	G3DSA:3.40.430.10. G3DSA:3.40.430.10. 1 hit.
PANTHER	PTHR11549:SF1. DHFR. 1 hit.
Pfam	PF00186. DHFR_1. 1 hit. [Graphical view]
PIRSF	PIRSF000194. DHFR. 1 hit.
PRINTS	PR00070. DHFR.
SUPFAM	SSF53597. SSF53597. 1 hit.
PROSITE	PS00075. DHFR_1. 1 hit. PS51330. DHFR_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DYR_AEDAL

Accession

Primary (citable) accession number: P28019

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	January 25, 2012
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)