Reviewed, UniProtKB/Swiss-Prot P28018 (BLA1_BACMY)

Names and origin

Protein names

Recommended name:
    Beta-lactamase 1
    EC=3.5.2.6
Alternative name(s):
    Beta-lactamase I
    Penicillinase

Gene names

Name:

blaCI

Organism

Bacillus mycoides

Taxonomic identifier

1405 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This protein is a beta-lactamase with a substrate specificity for penicillins.
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Sequence similarities	Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Domain	Signal
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 43	43	By similarity
	Chain	44 – 306	263	Beta-lactamase 1		PRO_0000016972
Regions
	Region	251 – 253	3	Substrate binding By similarity
Sites
	Active site	89	1	Acyl-ester intermediate By similarity
	Active site	185	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P28018-1 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 849BB01FD499E3BB	FASTA	306	33,383
	10 20 30 40 50 60 MKNKRMLKIG MCVGILGLSV TSLEAFTGGA LQVEAKEKTG QVKHKNQATH KEFSQLEKKF 70 80 90 100 110 120 DARLGVYAID TGTNQTISYR HNERFAFAST YKALAAGVLL QQNSIDTLNE VIKFTKEDLV 130 140 150 160 170 180 DYSPVTEKHV DTGMKLGEIA EAAVRSSDNT AGNILFHKIG GPKGYEKALR QIGDRVTMSD 190 200 210 220 230 240 RFETELNEAI PGDIRDTSTA KAIASNLKAF TVGNALPAEK RKILTEWMKG NATGDKLIRA 250 260 270 280 290 300 GVPTDWIVGD KSGAGSYGTR NDIAIVWPPN RAPIIIAILS SKDEKEATYD NQLIAEAPEV IVKSLK

References

[1]	"The beta-lactamase I gene From Bacillus mycoides." Groenstad A., Kristensen T., Hornes E., Kolstoe A.-B. Submitted (SEP-1991) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NI10R.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X62244 Genomic DNA. Translation: CAA44161.1.
PIR	S17339.
3D structure databases
HSSP	HSSP built from PDB template 1I2S based on UniProtKB P00808.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.5.2.6. 119335.
Family and domain databases
InterPro	IPR001466. Beta_lactamase-related. IPR000871. Beta_lactamase_A/D. [Graphical view]
Pfam	PF00144. Beta-lactamase. 1 hit. [Graphical view]
PRINTS	PR00118. BLACTAMASEA.
PROSITE	PS00146. BETA_LACTAMASE_A. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BLA1_BACMY

Accession

Primary (citable) accession number: P28018

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	June 16, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)