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P28012

- CFI1_MEDSA

UniProt

P28012 - CFI1_MEDSA

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Protein

Chalcone--flavonone isomerase 1

Gene
CHI1, CHI-1
Organism
Medicago sativa (Alfalfa)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the intramolecular cyclization of bicyclic chalcones into tricyclic (S)-flavanones. Responsible for the isomerization of 4,2',4',6'-tetrahydroxychalcone (also termed chalcone) into naringenin.3 Publications

Catalytic activityi

A chalcone = a flavanone.

Kineticsi

  1. KM=8.4 µM for 4,2',4'-trihydroxychalcone (at pH 7.5 and 25 degrees Celsius)3 Publications
  2. KM=15.7 µM for 6'-deoxychalcone (at pH 7.5 and 25 degrees Celsius)
  3. KM=22.7 µM for 2',4'-dihydroxychalcone (at pH 7.5 and 25 degrees Celsius)
  4. KM=42.5 µM for 4,2'-dihydroxychalcone (at pH 7.5 and 25 degrees Celsius)
  5. KM=112 µM for 4,2',4',6'-tetrahydroxychalcone (at pH 7.5 and 25 degrees Celsius)

pH dependencei

Optimum pH is 7-8.5 with 4,2',4'-trihydroxychalcone as substrate, at 25 degrees Celsius.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei48 – 481Substrate By similarity
Sitei106 – 1061Important for catalytic activity
Binding sitei113 – 1131Substrate By similarity
Binding sitei190 – 1901Substrate By similarity

GO - Molecular functioni

  1. chalcone isomerase activity Source: UniProtKB-EC

GO - Biological processi

  1. flavonoid biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Flavonoid biosynthesis

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-18291.
UniPathwayiUPA00154.

Names & Taxonomyi

Protein namesi
Recommended name:
Chalcone--flavonone isomerase 1 (EC:5.5.1.6)
Short name:
Chalcone isomerase 1
Gene namesi
Name:CHI1
Synonyms:CHI-1
OrganismiMedicago sativa (Alfalfa)
Taxonomic identifieri3879 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeTrifolieaeMedicago

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi48 – 481T → A: Strongly reduced reaction rate. 1 Publication
Mutagenesisi48 – 481T → S: Reduced reaction rate. 1 Publication
Mutagenesisi106 – 1061Y → F: Strongly reduced reaction rate. 2 Publications
Mutagenesisi113 – 1131N → A: Reduced reaction rate. 1 Publication
Mutagenesisi190 – 1901T → A: Reduced reaction rate. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 222222Chalcone--flavonone isomerase 1PRO_0000166434Add
BLAST

Proteomic databases

ProMEXiP28012.

Expressioni

Developmental stagei

Highest expression in young root tips.

Structurei

Secondary structure

1
222
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi8 – 103
Beta strandi13 – 153
Beta strandi17 – 204
Turni22 – 243
Beta strandi27 – 4014
Beta strandi43 – 5513
Helixi58 – 669
Helixi71 – 755
Helixi78 – 869
Beta strandi87 – 893
Beta strandi91 – 999
Helixi103 – 12018
Helixi126 – 13914
Beta strandi142 – 1443
Beta strandi149 – 1557
Turni156 – 1583
Beta strandi159 – 16911
Beta strandi175 – 1795
Helixi181 – 19212
Helixi200 – 21415

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EYPX-ray2.50A/B1-222[»]
1EYQX-ray1.85A/B1-222[»]
1FM7X-ray2.30A/B1-222[»]
1FM8X-ray2.30A/B1-222[»]
1JEPX-ray2.10A/B1-222[»]
1JX0X-ray2.85A/B1-222[»]
1JX1X-ray2.30A/B/C/D/E/F1-222[»]
ProteinModelPortaliP28012.
SMRiP28012. Positions 4-215.

Miscellaneous databases

EvolutionaryTraceiP28012.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.50.70.10. 1 hit.
InterProiIPR016087. Chalcone_isomerase.
IPR016088. Chalcone_isomerase_3-sand.
[Graphical view]
PfamiPF02431. Chalcone. 1 hit.
[Graphical view]
SUPFAMiSSF54626. SSF54626. 1 hit.

Sequencei

Sequence statusi: Complete.

P28012-1 [UniParc]FASTAAdd to Basket

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MAASITAITV ENLEYPAVVT SPVTGKSYFL GGAGERGLTI EGNFIKFTAI    50
GVYLEDIAVA SLAAKWKGKS SEELLETLDF YRDIISGPFE KLIRGSKIRE 100
LSGPEYSRKV MENCVAHLKS VGTYGDAEAE AMQKFAEAFK PVNFPPGASV 150
FYRQSPDGIL GLSFSPDTSI PEKEAALIEN KAVSSAVLET MIGEHAVSPD 200
LKRCLAARLP ALLNEGAFKI GN 222
Length:222
Mass (Da):23,826
Last modified:August 1, 1992 - v1
Checksum:i7767A72084AB4800
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M91079 mRNA. Translation: AAB41524.1.
PIRiS44371.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M91079 mRNA. Translation: AAB41524.1 .
PIRi S44371.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1EYP X-ray 2.50 A/B 1-222 [» ]
1EYQ X-ray 1.85 A/B 1-222 [» ]
1FM7 X-ray 2.30 A/B 1-222 [» ]
1FM8 X-ray 2.30 A/B 1-222 [» ]
1JEP X-ray 2.10 A/B 1-222 [» ]
1JX0 X-ray 2.85 A/B 1-222 [» ]
1JX1 X-ray 2.30 A/B/C/D/E/F 1-222 [» ]
ProteinModelPortali P28012.
SMRi P28012. Positions 4-215.
ModBasei Search...
MobiDBi Search...

Proteomic databases

ProMEXi P28012.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00154 .
BioCyci MetaCyc:MONOMER-18291.

Miscellaneous databases

EvolutionaryTracei P28012.

Family and domain databases

Gene3Di 3.50.70.10. 1 hit.
InterProi IPR016087. Chalcone_isomerase.
IPR016088. Chalcone_isomerase_3-sand.
[Graphical view ]
Pfami PF02431. Chalcone. 1 hit.
[Graphical view ]
SUPFAMi SSF54626. SSF54626. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Isolation of chalcone synthase and chalcone isomerase cDNAs from alfalfa (Medicago sativa L.): highest transcript levels occur in young roots and root tips."
    McKhann H.I., Hirsch A.M.
    Plant Mol. Biol. 24:767-777(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Iroquois.
  2. Erratum
    McKhann H.I., Hirsch A.M.
    Plant Mol. Biol. 25:759-759(1994)
  3. "Structure and mechanism of the evolutionarily unique plant enzyme chalcone isomerase."
    Jez J.M., Bowman M.E., Dixon R.A., Noel J.P.
    Nat. Struct. Biol. 7:786-791(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) IN COMPLEX WITH (2S)-NARINGENIN, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF TYR-106.
  4. "Role of hydrogen bonds in the reaction mechanism of chalcone isomerase."
    Jez J.M., Bowman M.E., Noel J.P.
    Biochemistry 41:5168-5176(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS), FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF THR-48; TYR-106; ASN-113 AND THR-190.
  5. "Reaction mechanism of chalcone isomerase. pH dependence, diffusion control, and product binding differences."
    Jez J.M., Noel J.P.
    J. Biol. Chem. 277:1361-1369(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS), FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiCFI1_MEDSA
AccessioniPrimary (citable) accession number: P28012
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: July 9, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Part of the biosynthetic pathway for all classes of flavonoids, a large class of secondary plant metabolites, many of which are brightly colored.

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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