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P28012

- CFI1_MEDSA

UniProt

P28012 - CFI1_MEDSA

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Protein

Chalcone--flavonone isomerase 1

Gene

CHI1

Organism
Medicago sativa (Alfalfa)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the intramolecular cyclization of bicyclic chalcones into tricyclic (S)-flavanones. Responsible for the isomerization of 4,2',4',6'-tetrahydroxychalcone (also termed chalcone) into naringenin.3 Publications

Catalytic activityi

A chalcone = a flavanone.

Kineticsi

  1. KM=8.4 µM for 4,2',4'-trihydroxychalcone (at pH 7.5 and 25 degrees Celsius)3 Publications
  2. KM=15.7 µM for 6'-deoxychalcone (at pH 7.5 and 25 degrees Celsius)3 Publications
  3. KM=22.7 µM for 2',4'-dihydroxychalcone (at pH 7.5 and 25 degrees Celsius)3 Publications
  4. KM=42.5 µM for 4,2'-dihydroxychalcone (at pH 7.5 and 25 degrees Celsius)3 Publications
  5. KM=112 µM for 4,2',4',6'-tetrahydroxychalcone (at pH 7.5 and 25 degrees Celsius)3 Publications

pH dependencei

Optimum pH is 7-8.5 with 4,2',4'-trihydroxychalcone as substrate, at 25 degrees Celsius.3 Publications

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei48 – 481SubstrateBy similarity
Sitei106 – 1061Important for catalytic activity
Binding sitei113 – 1131SubstrateBy similarity
Binding sitei190 – 1901SubstrateBy similarity

GO - Molecular functioni

  1. chalcone isomerase activity Source: UniProtKB-EC

GO - Biological processi

  1. flavonoid biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Flavonoid biosynthesis

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-18291.
SABIO-RKP28012.
UniPathwayiUPA00154.

Names & Taxonomyi

Protein namesi
Recommended name:
Chalcone--flavonone isomerase 1 (EC:5.5.1.6)
Short name:
Chalcone isomerase 1
Gene namesi
Name:CHI1
Synonyms:CHI-1
OrganismiMedicago sativa (Alfalfa)
Taxonomic identifieri3879 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeTrifolieaeMedicago

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi48 – 481T → A: Strongly reduced reaction rate. 1 Publication
Mutagenesisi48 – 481T → S: Reduced reaction rate. 1 Publication
Mutagenesisi106 – 1061Y → F: Strongly reduced reaction rate. 2 Publications
Mutagenesisi113 – 1131N → A: Reduced reaction rate. 1 Publication
Mutagenesisi190 – 1901T → A: Reduced reaction rate. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 222222Chalcone--flavonone isomerase 1PRO_0000166434Add
BLAST

Expressioni

Developmental stagei

Highest expression in young root tips.

Structurei

Secondary structure

1
222
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi8 – 103Combined sources
Beta strandi13 – 153Combined sources
Beta strandi17 – 204Combined sources
Turni22 – 243Combined sources
Beta strandi27 – 4014Combined sources
Beta strandi43 – 5513Combined sources
Helixi58 – 669Combined sources
Helixi71 – 755Combined sources
Helixi78 – 869Combined sources
Beta strandi87 – 893Combined sources
Beta strandi91 – 999Combined sources
Helixi103 – 12018Combined sources
Helixi126 – 13914Combined sources
Beta strandi142 – 1443Combined sources
Beta strandi149 – 1557Combined sources
Turni156 – 1583Combined sources
Beta strandi159 – 16911Combined sources
Beta strandi175 – 1795Combined sources
Helixi181 – 19212Combined sources
Helixi200 – 21415Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EYPX-ray2.50A/B1-222[»]
1EYQX-ray1.85A/B1-222[»]
1FM7X-ray2.30A/B1-222[»]
1FM8X-ray2.30A/B1-222[»]
1JEPX-ray2.10A/B1-222[»]
1JX0X-ray2.85A/B1-222[»]
1JX1X-ray2.30A/B/C/D/E/F1-222[»]
ProteinModelPortaliP28012.
SMRiP28012. Positions 4-215.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP28012.

Family & Domainsi

Sequence similaritiesi

Belongs to the chalcone isomerase family.Curated

Family and domain databases

Gene3Di3.50.70.10. 1 hit.
InterProiIPR016087. Chalcone_isomerase.
IPR016088. Chalcone_isomerase_3-sand.
[Graphical view]
PfamiPF02431. Chalcone. 1 hit.
[Graphical view]
SUPFAMiSSF54626. SSF54626. 1 hit.

Sequencei

Sequence statusi: Complete.

P28012-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAASITAITV ENLEYPAVVT SPVTGKSYFL GGAGERGLTI EGNFIKFTAI
60 70 80 90 100
GVYLEDIAVA SLAAKWKGKS SEELLETLDF YRDIISGPFE KLIRGSKIRE
110 120 130 140 150
LSGPEYSRKV MENCVAHLKS VGTYGDAEAE AMQKFAEAFK PVNFPPGASV
160 170 180 190 200
FYRQSPDGIL GLSFSPDTSI PEKEAALIEN KAVSSAVLET MIGEHAVSPD
210 220
LKRCLAARLP ALLNEGAFKI GN
Length:222
Mass (Da):23,826
Last modified:August 1, 1992 - v1
Checksum:i7767A72084AB4800
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M91079 mRNA. Translation: AAB41524.1.
PIRiS44371.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M91079 mRNA. Translation: AAB41524.1 .
PIRi S44371.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1EYP X-ray 2.50 A/B 1-222 [» ]
1EYQ X-ray 1.85 A/B 1-222 [» ]
1FM7 X-ray 2.30 A/B 1-222 [» ]
1FM8 X-ray 2.30 A/B 1-222 [» ]
1JEP X-ray 2.10 A/B 1-222 [» ]
1JX0 X-ray 2.85 A/B 1-222 [» ]
1JX1 X-ray 2.30 A/B/C/D/E/F 1-222 [» ]
ProteinModelPortali P28012.
SMRi P28012. Positions 4-215.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00154 .
BioCyci MetaCyc:MONOMER-18291.
SABIO-RK P28012.

Miscellaneous databases

EvolutionaryTracei P28012.

Family and domain databases

Gene3Di 3.50.70.10. 1 hit.
InterProi IPR016087. Chalcone_isomerase.
IPR016088. Chalcone_isomerase_3-sand.
[Graphical view ]
Pfami PF02431. Chalcone. 1 hit.
[Graphical view ]
SUPFAMi SSF54626. SSF54626. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Isolation of chalcone synthase and chalcone isomerase cDNAs from alfalfa (Medicago sativa L.): highest transcript levels occur in young roots and root tips."
    McKhann H.I., Hirsch A.M.
    Plant Mol. Biol. 24:767-777(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Iroquois.
  2. Erratum
    McKhann H.I., Hirsch A.M.
    Plant Mol. Biol. 25:759-759(1994)
  3. "Structure and mechanism of the evolutionarily unique plant enzyme chalcone isomerase."
    Jez J.M., Bowman M.E., Dixon R.A., Noel J.P.
    Nat. Struct. Biol. 7:786-791(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) IN COMPLEX WITH (2S)-NARINGENIN, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF TYR-106.
  4. "Role of hydrogen bonds in the reaction mechanism of chalcone isomerase."
    Jez J.M., Bowman M.E., Noel J.P.
    Biochemistry 41:5168-5176(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS), FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF THR-48; TYR-106; ASN-113 AND THR-190.
  5. "Reaction mechanism of chalcone isomerase. pH dependence, diffusion control, and product binding differences."
    Jez J.M., Noel J.P.
    J. Biol. Chem. 277:1361-1369(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS), FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiCFI1_MEDSA
AccessioniPrimary (citable) accession number: P28012
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: November 26, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Part of the biosynthetic pathway for all classes of flavonoids, a large class of secondary plant metabolites, many of which are brightly colored.

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3