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P28012

- CFI1_MEDSA

UniProt

P28012 - CFI1_MEDSA

Protein

Chalcone--flavonone isomerase 1

Gene

CHI1

Organism
Medicago sativa (Alfalfa)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (01 Aug 1992)
      Previous versions | rss
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    Functioni

    Catalyzes the intramolecular cyclization of bicyclic chalcones into tricyclic (S)-flavanones. Responsible for the isomerization of 4,2',4',6'-tetrahydroxychalcone (also termed chalcone) into naringenin.3 Publications

    Catalytic activityi

    A chalcone = a flavanone.

    Kineticsi

    1. KM=8.4 µM for 4,2',4'-trihydroxychalcone (at pH 7.5 and 25 degrees Celsius)3 Publications
    2. KM=15.7 µM for 6'-deoxychalcone (at pH 7.5 and 25 degrees Celsius)3 Publications
    3. KM=22.7 µM for 2',4'-dihydroxychalcone (at pH 7.5 and 25 degrees Celsius)3 Publications
    4. KM=42.5 µM for 4,2'-dihydroxychalcone (at pH 7.5 and 25 degrees Celsius)3 Publications
    5. KM=112 µM for 4,2',4',6'-tetrahydroxychalcone (at pH 7.5 and 25 degrees Celsius)3 Publications

    pH dependencei

    Optimum pH is 7-8.5 with 4,2',4'-trihydroxychalcone as substrate, at 25 degrees Celsius.3 Publications

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei48 – 481SubstrateBy similarity
    Sitei106 – 1061Important for catalytic activity
    Binding sitei113 – 1131SubstrateBy similarity
    Binding sitei190 – 1901SubstrateBy similarity

    GO - Molecular functioni

    1. chalcone isomerase activity Source: UniProtKB-EC

    GO - Biological processi

    1. flavonoid biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Flavonoid biosynthesis

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-18291.
    UniPathwayiUPA00154.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chalcone--flavonone isomerase 1 (EC:5.5.1.6)
    Short name:
    Chalcone isomerase 1
    Gene namesi
    Name:CHI1
    Synonyms:CHI-1
    OrganismiMedicago sativa (Alfalfa)
    Taxonomic identifieri3879 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeTrifolieaeMedicago

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi48 – 481T → A: Strongly reduced reaction rate. 1 Publication
    Mutagenesisi48 – 481T → S: Reduced reaction rate. 1 Publication
    Mutagenesisi106 – 1061Y → F: Strongly reduced reaction rate. 2 Publications
    Mutagenesisi113 – 1131N → A: Reduced reaction rate. 1 Publication
    Mutagenesisi190 – 1901T → A: Reduced reaction rate. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 222222Chalcone--flavonone isomerase 1PRO_0000166434Add
    BLAST

    Proteomic databases

    ProMEXiP28012.

    Expressioni

    Developmental stagei

    Highest expression in young root tips.

    Structurei

    Secondary structure

    1
    222
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi8 – 103
    Beta strandi13 – 153
    Beta strandi17 – 204
    Turni22 – 243
    Beta strandi27 – 4014
    Beta strandi43 – 5513
    Helixi58 – 669
    Helixi71 – 755
    Helixi78 – 869
    Beta strandi87 – 893
    Beta strandi91 – 999
    Helixi103 – 12018
    Helixi126 – 13914
    Beta strandi142 – 1443
    Beta strandi149 – 1557
    Turni156 – 1583
    Beta strandi159 – 16911
    Beta strandi175 – 1795
    Helixi181 – 19212
    Helixi200 – 21415

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1EYPX-ray2.50A/B1-222[»]
    1EYQX-ray1.85A/B1-222[»]
    1FM7X-ray2.30A/B1-222[»]
    1FM8X-ray2.30A/B1-222[»]
    1JEPX-ray2.10A/B1-222[»]
    1JX0X-ray2.85A/B1-222[»]
    1JX1X-ray2.30A/B/C/D/E/F1-222[»]
    ProteinModelPortaliP28012.
    SMRiP28012. Positions 4-215.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP28012.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the chalcone isomerase family.Curated

    Family and domain databases

    Gene3Di3.50.70.10. 1 hit.
    InterProiIPR016087. Chalcone_isomerase.
    IPR016088. Chalcone_isomerase_3-sand.
    [Graphical view]
    PfamiPF02431. Chalcone. 1 hit.
    [Graphical view]
    SUPFAMiSSF54626. SSF54626. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P28012-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAASITAITV ENLEYPAVVT SPVTGKSYFL GGAGERGLTI EGNFIKFTAI    50
    GVYLEDIAVA SLAAKWKGKS SEELLETLDF YRDIISGPFE KLIRGSKIRE 100
    LSGPEYSRKV MENCVAHLKS VGTYGDAEAE AMQKFAEAFK PVNFPPGASV 150
    FYRQSPDGIL GLSFSPDTSI PEKEAALIEN KAVSSAVLET MIGEHAVSPD 200
    LKRCLAARLP ALLNEGAFKI GN 222
    Length:222
    Mass (Da):23,826
    Last modified:August 1, 1992 - v1
    Checksum:i7767A72084AB4800
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M91079 mRNA. Translation: AAB41524.1.
    PIRiS44371.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M91079 mRNA. Translation: AAB41524.1 .
    PIRi S44371.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1EYP X-ray 2.50 A/B 1-222 [» ]
    1EYQ X-ray 1.85 A/B 1-222 [» ]
    1FM7 X-ray 2.30 A/B 1-222 [» ]
    1FM8 X-ray 2.30 A/B 1-222 [» ]
    1JEP X-ray 2.10 A/B 1-222 [» ]
    1JX0 X-ray 2.85 A/B 1-222 [» ]
    1JX1 X-ray 2.30 A/B/C/D/E/F 1-222 [» ]
    ProteinModelPortali P28012.
    SMRi P28012. Positions 4-215.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    ProMEXi P28012.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00154 .
    BioCyci MetaCyc:MONOMER-18291.

    Miscellaneous databases

    EvolutionaryTracei P28012.

    Family and domain databases

    Gene3Di 3.50.70.10. 1 hit.
    InterProi IPR016087. Chalcone_isomerase.
    IPR016088. Chalcone_isomerase_3-sand.
    [Graphical view ]
    Pfami PF02431. Chalcone. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54626. SSF54626. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Isolation of chalcone synthase and chalcone isomerase cDNAs from alfalfa (Medicago sativa L.): highest transcript levels occur in young roots and root tips."
      McKhann H.I., Hirsch A.M.
      Plant Mol. Biol. 24:767-777(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Iroquois.
    2. Erratum
      McKhann H.I., Hirsch A.M.
      Plant Mol. Biol. 25:759-759(1994)
    3. "Structure and mechanism of the evolutionarily unique plant enzyme chalcone isomerase."
      Jez J.M., Bowman M.E., Dixon R.A., Noel J.P.
      Nat. Struct. Biol. 7:786-791(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) IN COMPLEX WITH (2S)-NARINGENIN, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF TYR-106.
    4. "Role of hydrogen bonds in the reaction mechanism of chalcone isomerase."
      Jez J.M., Bowman M.E., Noel J.P.
      Biochemistry 41:5168-5176(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS), FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF THR-48; TYR-106; ASN-113 AND THR-190.
    5. "Reaction mechanism of chalcone isomerase. pH dependence, diffusion control, and product binding differences."
      Jez J.M., Noel J.P.
      J. Biol. Chem. 277:1361-1369(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS), FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.

    Entry informationi

    Entry nameiCFI1_MEDSA
    AccessioniPrimary (citable) accession number: P28012
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: August 1, 1992
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Part of the biosynthetic pathway for all classes of flavonoids, a large class of secondary plant metabolites, many of which are brightly colored.

    Keywords - Technical termi

    3D-structure

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3