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Protein

H/ACA ribonucleoprotein complex subunit 1

Gene

GAR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for ribosome biogenesis. Part of a complex which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Pseudouridine ("psi") residues may serve to stabilize the conformation of rRNAs. Essential for growth.1 Publication

GO - Molecular functioni

  • box H/ACA snoRNA binding Source: SGD

GO - Biological processi

  • rRNA processing Source: UniProtKB-KW
  • snRNA pseudouridine synthesis Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

Ribosome biogenesis, rRNA processing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31136-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
H/ACA ribonucleoprotein complex subunit 1
Alternative name(s):
snoRNP protein GAR1
Gene namesi
Name:GAR1
Ordered Locus Names:YHR089C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR089C.
SGDiS000001131. GAR1.

Subcellular locationi

GO - Cellular componenti

  • box H/ACA snoRNP complex Source: UniProtKB
  • nucleolus Source: SGD
  • small nucleolar ribonucleoprotein complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 205205H/ACA ribonucleoprotein complex subunit 1PRO_0000208566Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei4 – 41Asymmetric dimethylarginine1 Publication
Modified residuei8 – 81Asymmetric dimethylarginine1 Publication
Modified residuei11 – 111Asymmetric dimethylarginine1 Publication
Modified residuei15 – 151Asymmetric dimethylarginine1 Publication
Modified residuei19 – 191Asymmetric dimethylarginine1 Publication
Modified residuei147 – 1471Asymmetric dimethylarginine1 Publication
Modified residuei154 – 1541Asymmetric dimethylarginine1 Publication
Modified residuei158 – 1581Asymmetric dimethylarginine1 Publication
Modified residuei162 – 1621Asymmetric dimethylarginine1 Publication
Modified residuei165 – 1651Asymmetric dimethylarginine1 Publication
Modified residuei171 – 1711Asymmetric dimethylarginine1 Publication
Modified residuei174 – 1741Asymmetric dimethylarginine1 Publication
Modified residuei180 – 1801Asymmetric dimethylarginine1 Publication
Modified residuei184 – 1841Asymmetric dimethylarginine1 Publication
Modified residuei189 – 1891Asymmetric dimethylarginine1 Publication
Modified residuei193 – 1931Asymmetric dimethylarginine1 Publication
Modified residuei197 – 1971Asymmetric dimethylarginine1 Publication
Modified residuei201 – 2011Asymmetric dimethylarginine1 Publication

Post-translational modificationi

Methylated by HMT1, forming asymmetric dimethylarginines (DMA) within a domain referred to as an RGG box, made up of repeated Gly-Gly dipeptides interspersed with Arg and aromatic residues.1 Publication

Keywords - PTMi

Methylation

Proteomic databases

MaxQBiP28007.
PeptideAtlasiP28007.
PRIDEiP28007.

Interactioni

Subunit structurei

Component of the small nucleolar ribonucleoprotein particles containing H/ACA-type snoRNAs (H/ACA snoRNPs). The protein component of the H/ACA snoRNP contains CBF5, GAR1, NHP2 and NOP10. The complex contains a stable core composed of CBF5 and NOP10, to which GAR1 and NHP2 subsequently bind. Interacts with snoRNAs.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
NHP2P324953EBI-7321,EBI-12014

Protein-protein interaction databases

BioGridi36524. 98 interactions.
DIPiDIP-4484N.
IntActiP28007. 79 interactions.
MINTiMINT-551032.

Structurei

Secondary structure

1
205
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi37 – 4711Combined sources
Beta strandi50 – 556Combined sources
Beta strandi57 – 615Combined sources
Beta strandi66 – 683Combined sources
Beta strandi74 – 8411Combined sources
Beta strandi89 – 946Combined sources
Helixi100 – 1023Combined sources
Beta strandi108 – 1125Combined sources
Helixi113 – 1153Combined sources
Helixi119 – 1224Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3U28X-ray1.90C32-124[»]
3UAIX-ray3.06C32-124[»]
ProteinModelPortaliP28007.
SMRiP28007. Positions 33-124.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni4 – 2118RGG-box 1Add
BLAST
Regioni147 – 20559RGG-box 2Add
BLAST

Sequence similaritiesi

Belongs to the GAR1 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00730000111223.
InParanoidiP28007.
KOiK11128.
OMAiHFNAQIF.
OrthoDBiEOG7M0P4G.

Family and domain databases

InterProiIPR021154. H/ACA_rnp_Gar1.
IPR007504. H/ACA_rnp_Gar1/Naf1.
IPR009000. Transl_B-barrel.
[Graphical view]
PANTHERiPTHR23237. PTHR23237. 1 hit.
PfamiPF04410. Gar1. 1 hit.
[Graphical view]
ProDomiPD020235. Gar1_RNA_bd. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF50447. SSF50447. 1 hit.

Sequencei

Sequence statusi: Complete.

P28007-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFRGGNRGG RGGFRGGFRG GRTGSARSFQ QGPPDTVLEM GAFLHPCEGD
60 70 80 90 100
IVCRSINTKI PYFNAPIYLE NKTQVGKVDE ILGPLNEVFF TIKCGDGVQA
110 120 130 140 150
TSFKEGDKFY IAADKLLPIE RFLPKPKVVG PPKPKNKKKR SGAPGGRGGA
160 170 180 190 200
SMGRGGSRGG FRGGRGGSSF RGGRGGSSFR GGSRGGSFRG GSRGGSRGGF

RGGRR
Length:205
Mass (Da):21,480
Last modified:August 1, 1992 - v1
Checksum:iC197FE85644DD0F8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63617 Genomic DNA. Translation: CAA45162.1.
U00060 Genomic DNA. Translation: AAB68929.1.
AY558284 Genomic DNA. Translation: AAS56610.1.
BK006934 Genomic DNA. Translation: DAA06785.1.
PIRiS19634.
RefSeqiNP_011957.1. NM_001179219.1.

Genome annotation databases

EnsemblFungiiYHR089C; YHR089C; YHR089C.
GeneIDi856489.
KEGGisce:YHR089C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63617 Genomic DNA. Translation: CAA45162.1.
U00060 Genomic DNA. Translation: AAB68929.1.
AY558284 Genomic DNA. Translation: AAS56610.1.
BK006934 Genomic DNA. Translation: DAA06785.1.
PIRiS19634.
RefSeqiNP_011957.1. NM_001179219.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3U28X-ray1.90C32-124[»]
3UAIX-ray3.06C32-124[»]
ProteinModelPortaliP28007.
SMRiP28007. Positions 33-124.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36524. 98 interactions.
DIPiDIP-4484N.
IntActiP28007. 79 interactions.
MINTiMINT-551032.

Proteomic databases

MaxQBiP28007.
PeptideAtlasiP28007.
PRIDEiP28007.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR089C; YHR089C; YHR089C.
GeneIDi856489.
KEGGisce:YHR089C.

Organism-specific databases

EuPathDBiFungiDB:YHR089C.
SGDiS000001131. GAR1.

Phylogenomic databases

GeneTreeiENSGT00730000111223.
InParanoidiP28007.
KOiK11128.
OMAiHFNAQIF.
OrthoDBiEOG7M0P4G.

Enzyme and pathway databases

BioCyciYEAST:G3O-31136-MONOMER.

Miscellaneous databases

PROiP28007.

Family and domain databases

InterProiIPR021154. H/ACA_rnp_Gar1.
IPR007504. H/ACA_rnp_Gar1/Naf1.
IPR009000. Transl_B-barrel.
[Graphical view]
PANTHERiPTHR23237. PTHR23237. 1 hit.
PfamiPF04410. Gar1. 1 hit.
[Graphical view]
ProDomiPD020235. Gar1_RNA_bd. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF50447. SSF50447. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "GAR1 is an essential small nucleolar RNP protein required for pre-rRNA processing in yeast."
    Girard J.-P., Lehtonen H., Caizergues-Ferrer M., Amalric F., Tollervey D., Lapeyre B.
    EMBO J. 11:673-682(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: YNN 281.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "A small nucleolar RNP protein is required for pseudouridylation of eukaryotic ribosomal RNAs."
    Bousquet-Antonelli C., Henry Y., Gelugne J.-P., Caizergues-Ferrer M., Kiss T.
    EMBO J. 16:4770-4776(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. Cited for: SUBCELLULAR LOCATION, IDENTIFICATION IN H/ACA SNORNP COMPLEXES.
  7. "Gar1p binds to the small nucleolar RNAs snR10 and snR30 in vitro through a nontypical RNA binding element."
    Bagni C., Lapeyre B.
    J. Biol. Chem. 273:10868-10873(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH H/ACA SNORNAS.
  8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  10. "In vivo analysis of nucleolar proteins modified by the yeast arginine methyltransferase Hmt1/Rmt1p."
    Xu C., Henry P.A., Setya A., Henry M.F.
    RNA 9:746-759(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, METHYLATION BY HMT1.
  11. "Cbf5p, the putative pseudouridine synthase of H/ACA-type snoRNPs, can form a complex with Gar1p and Nop10p in absence of Nhp2p and box H/ACA snoRNAs."
    Henras A.K., Capeyrou R., Henry Y., Caizergues-Ferrer M.
    RNA 10:1704-1712(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION OF THE H/ACA SNORNP COMPLEX.
  12. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "Structure of the Shq1-Cbf5-Nop10-Gar1 complex and implications for H/ACA RNP biogenesis and dyskeratosis congenita."
    Li S., Duan J., Li D., Ma S., Ye K.
    EMBO J. 30:5010-5020(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.06 ANGSTROMS) OF 32-124 IN A COMPLEX WITH CBF5; NOP10 AND SHQ1.
  14. "Reconstitution and structural analysis of the yeast box H/ACA RNA-guided pseudouridine synthase."
    Li S., Duan J., Li D., Yang B., Dong M., Ye K.
    Genes Dev. 25:2409-2421(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) OF 32-124 IN A COMPLEX WITH CBF5 AND NOP10.

Entry informationi

Entry nameiGAR1_YEAST
AccessioniPrimary (citable) accession number: P28007
Secondary accession number(s): D3DL41
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: June 8, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5730 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.