Reviewed, UniProtKB/Swiss-Prot P28007 (GAR1_YEAST)

Names and origin

Protein names

Recommended name:
    H/ACA ribonucleoprotein complex subunit 1
Alternative name(s):
    snoRNP protein GAR1

Gene names

Name:	GAR1
Ordered Locus Names:	YHR089C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	205 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for ribosome biogenesis. Part of a complex which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Pseudouridine ("psi") residues may serve to stabilize the conformation of rRNAs. Essential for growth. Ref.4
Subunit structure	Component of the small nucleolar ribonucleoprotein particles containing H/ACA-type snoRNAs (H/ACA snoRNPs). The protein component of the H/ACA snoRNP contains CBF5, GAR1, NHP2 and NOP10. The complex contains a stable core composed of CBF5 and NOP10, to which GAR1 and NHP2 subsequently bind. Interacts with snoRNAs. Ref.6
Subcellular location	Nucleus › nucleolus.
Post-translational modification	Asymmetric arginine dimethylation by HMT1.
Miscellaneous	Present with 5730 molecules/cell in log phase SD medium. Ref.8
Sequence similarities	Belongs to the GAR1 family.

Ontologies

Keywords
Biological process	Ribosome biogenesis rRNA processing
Cellular component	Nucleus
Domain	Repeat
Ligand	RNA-binding
Molecular function	Ribonucleoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA processing Traceable author statement. Source: SGD snRNA pseudouridine synthesis Inferred from direct assay. Source: SGD
Cellular component	box H/ACA snoRNP complex Ref.5 Inferred from physical interaction. Source: UniProtKB
Molecular function	box H/ACA snoRNA binding Ref.6 Inferred from physical interaction. Source: SGD identical protein binding Inferred from physical interaction. Source: IntAct rRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-7321,EBI-7321
BRX1	Q08235	1	EBI-7321,EBI-3775
DBP2	P24783	1	EBI-7321,EBI-5602
DBP3	P20447	1	EBI-7321,EBI-5608
FPR4	Q06205	1	EBI-7321,EBI-6956
GIS2	P53849	1	EBI-7321,EBI-29244
LRP1	P38801	1	EBI-7321,EBI-1909
MBF1	O14467	1	EBI-7321,EBI-2043025
NHP2	P32495	1	EBI-7321,EBI-12014
NOC2	P39744	1	EBI-7321,EBI-29259
NOP10	Q6Q547	1	EBI-7321,EBI-2052475
NSR1	P27476	1	EBI-7321,EBI-12274
PRP43	P53131	1	EBI-7321,EBI-505
PWP1	P21304	1	EBI-7321,EBI-14328
PXR1	P53335	1	EBI-7321,EBI-23652
RPA1	P10964	1	EBI-7321,EBI-15730
RPA43	P46669	1	EBI-7321,EBI-15745
SRP40	P32583	1	EBI-7321,EBI-17992
TMA23	Q03525	1	EBI-7321,EBI-27597
UBP10	P53874	1	EBI-7321,EBI-19873
UTP23	Q12339	1	EBI-7321,EBI-30231

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 205	205	H/ACA ribonucleoprotein complex subunit 1		PRO_0000208566
Regions
	Region	4 – 21	18	RGG-box 1
	Region	147 – 205	59	RGG-box 2

Sequences

	Sequence		Length	Mass (Da)
	P28007-1 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: C197FE85644DD0F8	FASTA	205	21,480
	10 20 30 40 50 60 MSFRGGNRGG RGGFRGGFRG GRTGSARSFQ QGPPDTVLEM GAFLHPCEGD IVCRSINTKI 70 80 90 100 110 120 PYFNAPIYLE NKTQVGKVDE ILGPLNEVFF TIKCGDGVQA TSFKEGDKFY IAADKLLPIE 130 140 150 160 170 180 RFLPKPKVVG PPKPKNKKKR SGAPGGRGGA SMGRGGSRGG FRGGRGGSSF RGGRGGSSFR 190 200 GGSRGGSFRG GSRGGSRGGF RGGRR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"GAR1 is an essential small nucleolar RNP protein required for pre-rRNA processing in yeast." Girard J.-P., Lehtonen H., Caizergues-Ferrer M., Amalric F., Tollervey D., Lapeyre B. EMBO J. 11:673-682(1992) [PubMed: 1531632] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: YNN 281.
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"A small nucleolar RNP protein is required for pseudouridylation of eukaryotic ribosomal RNAs." Bousquet-Antonelli C., Henry Y., Gelugne J.-P., Caizergues-Ferrer M., Kiss T. EMBO J. 16:4770-4776(1997) [PubMed: 9303321] [Abstract] Cited for: FUNCTION.
[5]	"Nhp2p and Nop10p are essential for the function of H/ACA snoRNPs." Henras A., Henry Y., Bousquet-Antonelli C., Noaillac-Depeyre J., Gelugne J.-P., Caizergues-Ferrer M. EMBO J. 17:7078-7090(1998) [PubMed: 9843512] [Abstract] Cited for: SUBCELLULAR LOCATION, IDENTIFICATION IN H/ACA SNORNP COMPLEXES.
[6]	"Gar1p binds to the small nucleolar RNAs snR10 and snR30 in vitro through a nontypical RNA binding element." Bagni C., Lapeyre B. J. Biol. Chem. 273:10868-10873(1998) [PubMed: 9556561] [Abstract] Cited for: INTERACTION WITH H/ACA SNORNAS.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"In vivo analysis of nucleolar proteins modified by the yeast arginine methyltransferase Hmt1/Rmt1p." Xu C., Henry P.A., Setya A., Henry M.F. RNA 9:746-759(2003) [PubMed: 12756332] [Abstract] Cited for: SUBCELLULAR LOCATION, METHYLATION BY HMT1.
[10]	"Cbf5p, the putative pseudouridine synthase of H/ACA-type snoRNPs, can form a complex with Gar1p and Nop10p in absence of Nhp2p and box H/ACA snoRNAs." Henras A.K., Capeyrou R., Henry Y., Caizergues-Ferrer M. RNA 10:1704-1712(2004) [PubMed: 15388873] [Abstract] Cited for: CHARACTERIZATION OF THE H/ACA SNORNP COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X63617 Genomic DNA. Translation: CAA45162.1. U00060 Genomic DNA. Translation: AAB68929.1. AY558284 Genomic DNA. Translation: AAS56610.1.
PIR	S19634.
RefSeq	NP_011957.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4484N.
IntAct	P28007. 77 interactions.
STRING	P28007.
Proteomic databases
PeptideAtlas	P28007.
PRIDE	P28007.
Genome annotation databases
Ensembl	YHR089C; YHR089C; YHR089C; Saccharomyces cerevisiae. [Genome view]
GeneID	856489.
KEGG	sce:YHR089C.
NMPDR	fig|4932.3.peg.3118.
Organism-specific databases
CYGD	YHR089c.
SGD	S000001131. GAR1.
Phylogenomic databases
HOGENOM	P28007.
OMA	IYLENKT
OrthoDB	EOG91K0VJ
Gene expression databases
ArrayExpress	P28007.
Genevestigator	P28007.
GermOnline	YHR089C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007504. Gar1_RNA-bd_reg. [Graphical view]
PANTHER	PTHR23237. Gar1_RNA_bd. 1 hit.
Pfam	PF04410. Gar1. 1 hit. [Graphical view]
ProDom	PD020235. Gar1_RNA_bd. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...
Other Resources
NextBio	982191.

Entry information

Entry name

GAR1_YEAST

Accession

Primary (citable) accession number: P28007

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	November 24, 2009
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)