P28005 (POP5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonucleases P/MRP protein subunit POP5
EC=3.1.26.5

Alternative name(s):
RNA-processing protein POP5
RNases P/MRP 19.6 kDa subunit

Gene names

Name:	POP5
Ordered Locus Names:	YAL033W
ORF Names:	FUN53

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	173 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of RNase MRP, which cleaves pre-rRNA sequences. Ref.4
Catalytic activity	Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.
Subunit structure	Component of nuclear RNase P and RNase MRP complexes. RNase P consists of an RNA moiety and at least 9 protein subunits including POP1, POP3, POP4, POP5, POP6, POP7, POP8, RPP1 and RPR2. RNase MRP complex consists of an RNA moiety and at least 10 protein subunits including POP1, POP3, POP4, POP5, POP6, POP7, POP8, RMP1, RPP1 and SNM1, many of which are shared with the RNase P complex.
Subcellular location	Cytoplasm. Nucleus Ref.5.
Miscellaneous	Present with 2230 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the eukaryotic/archaeal RNase P protein component 2 family.

Ontologies

Keywords
Biological process	rRNA processing tRNA processing
Cellular component	Cytoplasm Nucleus
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	intronic box C/D snoRNA processing Inferred from direct assay. Source: SGD mRNA cleavage Inferred from physical interaction. Source: SGD rRNA processing Inferred from direct assay. Source: MGI tRNA processing Traceable author statement Ref.4. Source: SGD
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleolar ribonuclease P complex Inferred from direct assay Ref.4. Source: SGD ribonuclease MRP complex Inferred from direct assay. Source: MGI
Molecular function	ribonuclease MRP activity Inferred from direct assay. Source: MGI ribonuclease P activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 173	173	Ribonucleases P/MRP protein subunit POP5		PRO_0000140015

Sequences

	Sequence		Length	Mass (Da)
	P28005 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 918193631BD790DD	FASTA	173	19,574
	10 20 30 40 50 60 MVRLKSRYIL FEIIFPPTDT NVEESVSKAD ILLSHHRASP ADVSIKSILQ EIRRSLSLNL 70 80 90 100 110 120 GDYGSAKCNS LLQLKYFSNK TSTGIIRCHR EDCDLVIMAL MLMSKIGDVD GLIVNPVKVS 130 140 150 160 170 GTIKKIEQFA MRRNSKILNI IKCSQSSHLS DNDFIINDFK KIGRENENEN EDD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular analysis of Saccharomyces cerevisiae chromosome I. On the number of genes and the identification of essential genes using temperature-sensitive-lethal mutations." Harris S.D., Cheng J., Pugh T.A., Pringle J.R. J. Mol. Biol. 225:53-65(1992) [PubMed: 1583694] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae." Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K. Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed: 7731988] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Purification and characterization of the nuclear RNase P holoenzyme complex reveals extensive subunit overlap with RNase MRP." Chamberlain J.R., Lee Y., Lane W.S., Engelke D.R. Genes Dev. 12:1678-1690(1998) [PubMed: 9620854] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE RNASE P COMPLEX BY MASS SPECTROMETRY.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Characterization and purification of Saccharomyces cerevisiae RNase MRP reveals a new unique protein component." Salinas K., Wierzbicki S., Zhou L., Schmitt M.E. J. Biol. Chem. 280:11352-11360(2005) [PubMed: 15637077] [Abstract] Cited for: IDENTIFICATION IN THE RNASE MRP COMPLEX BY MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X62577 Genomic DNA. Translation: CAA44457.1. U12980 Genomic DNA. Translation: AAC04999.1. BK006935 Genomic DNA. Translation: DAA06955.1.
PIR	S23411.
RefSeq	NP_009369.1. NM_001178178.1.
3D structure databases
ProteinModelPortal	P28005.
SMR	P28005. Positions 1-135.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3789N.
IntAct	P28005. 7 interactions.
MINT	MINT-573609.
STRING	P28005.
Proteomic databases
PeptideAtlas	P28005.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YAL033W; YAL033W; YAL033W.
GeneID	851200.
KEGG	sce:YAL033W.
NMPDR	fig|4932.3.peg.48.
Organism-specific databases
CYGD	YAL033w.
SGD	S000000031. POP5.
Phylogenomic databases
eggNOG	fuNOG10677.
GeneTree	EFGT00050000005133.
HOGENOM	HBG396624.
OMA	YFSNKTS.
OrthoDB	EOG4M9522.
Gene expression databases
ArrayExpress	P28005.
Genevestigator	P28005.
GermOnline	YAL033W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002759. RNase_P/MRP_subunit. [Graphical view]
KO	K03537.
Pfam	PF01900. RNase_P_Rpp14. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	968055.

Entry information

Entry name

POP5_YEAST

Accession

Primary (citable) accession number: P28005
Secondary accession number(s): D6VPI5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	December 14, 2011
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program