Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ribonuclease P/MRP protein subunit POP5

Gene

POP5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of RNase MRP, which cleaves pre-rRNA sequences.1 Publication

Catalytic activityi

Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.

GO - Molecular functioni

  • ribonuclease MRP activity Source: MGI
  • ribonuclease P activity Source: UniProtKB-EC
  • RNA binding Source: SGD

GO - Biological processi

  • intronic box C/D snoRNA processing Source: SGD
  • mRNA cleavage Source: SGD
  • RNA phosphodiester bond hydrolysis Source: GOC
  • RNA phosphodiester bond hydrolysis, endonucleolytic Source: GOC
  • rRNA processing Source: SGD
  • tRNA processing Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

rRNA processing, tRNA processing

Enzyme and pathway databases

BioCyciYEAST:YAL033W-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease P/MRP protein subunit POP5 (EC:3.1.26.5)
Alternative name(s):
RNA-processing protein POP5
RNase P/MRP 19.6 kDa subunit
Gene namesi
Name:POP5
Ordered Locus Names:YAL033W
ORF Names:FUN53
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome I

Organism-specific databases

CYGDiYAL033w.
EuPathDBiFungiDB:YAL033W.
SGDiS000000031. POP5.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nucleolar ribonuclease P complex Source: SGD
  • ribonuclease MRP complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 173173Ribonuclease P/MRP protein subunit POP5PRO_0000140015Add
BLAST

Proteomic databases

MaxQBiP28005.
PaxDbiP28005.
PeptideAtlasiP28005.

Interactioni

Subunit structurei

Component of nuclear RNase P and RNase MRP complexes. RNase P consists of an RNA moiety and at least 9 protein subunits including POP1, POP3, POP4, POP5, POP6, POP7, POP8, RPP1 and RPR2. RNase MRP complex consists of an RNA moiety and at least 10 protein subunits including POP1, POP3, POP4, POP5, POP6, POP7, POP8, RMP1, RPP1 and SNM1, many of which are shared with the RNase P complex.2 Publications

Protein-protein interaction databases

BioGridi31733. 21 interactions.
DIPiDIP-3789N.
IntActiP28005. 7 interactions.
MINTiMINT-573609.
STRINGi4932.YAL033W.

Structurei

3D structure databases

ProteinModelPortaliP28005.
SMRiP28005. Positions 1-135.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1369.
HOGENOMiHOG000216538.
InParanoidiP28005.
KOiK03537.
OMAiGTIKKCE.
OrthoDBiEOG7M6DM5.

Family and domain databases

InterProiIPR016819. RNase_P/MRP_POP5.
IPR002759. RNase_P/MRP_subunit.
[Graphical view]
PfamiPF01900. RNase_P_Rpp14. 1 hit.
[Graphical view]
PIRSFiPIRSF023803. Ribonuclease_P_prd. 1 hit.

Sequencei

Sequence statusi: Complete.

P28005-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVRLKSRYIL FEIIFPPTDT NVEESVSKAD ILLSHHRASP ADVSIKSILQ
60 70 80 90 100
EIRRSLSLNL GDYGSAKCNS LLQLKYFSNK TSTGIIRCHR EDCDLVIMAL
110 120 130 140 150
MLMSKIGDVD GLIVNPVKVS GTIKKIEQFA MRRNSKILNI IKCSQSSHLS
160 170
DNDFIINDFK KIGRENENEN EDD
Length:173
Mass (Da):19,574
Last modified:August 1, 1992 - v1
Checksum:i918193631BD790DD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62577 Genomic DNA. Translation: CAA44457.1.
U12980 Genomic DNA. Translation: AAC04999.1.
BK006935 Genomic DNA. Translation: DAA06955.1.
PIRiS23411.
RefSeqiNP_009369.1. NM_001178178.1.

Genome annotation databases

EnsemblFungiiYAL033W; YAL033W; YAL033W.
GeneIDi851200.
KEGGisce:YAL033W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62577 Genomic DNA. Translation: CAA44457.1.
U12980 Genomic DNA. Translation: AAC04999.1.
BK006935 Genomic DNA. Translation: DAA06955.1.
PIRiS23411.
RefSeqiNP_009369.1. NM_001178178.1.

3D structure databases

ProteinModelPortaliP28005.
SMRiP28005. Positions 1-135.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31733. 21 interactions.
DIPiDIP-3789N.
IntActiP28005. 7 interactions.
MINTiMINT-573609.
STRINGi4932.YAL033W.

Proteomic databases

MaxQBiP28005.
PaxDbiP28005.
PeptideAtlasiP28005.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYAL033W; YAL033W; YAL033W.
GeneIDi851200.
KEGGisce:YAL033W.

Organism-specific databases

CYGDiYAL033w.
EuPathDBiFungiDB:YAL033W.
SGDiS000000031. POP5.

Phylogenomic databases

eggNOGiCOG1369.
HOGENOMiHOG000216538.
InParanoidiP28005.
KOiK03537.
OMAiGTIKKCE.
OrthoDBiEOG7M6DM5.

Enzyme and pathway databases

BioCyciYEAST:YAL033W-MONOMER.

Miscellaneous databases

NextBioi968055.
PROiP28005.

Family and domain databases

InterProiIPR016819. RNase_P/MRP_POP5.
IPR002759. RNase_P/MRP_subunit.
[Graphical view]
PfamiPF01900. RNase_P_Rpp14. 1 hit.
[Graphical view]
PIRSFiPIRSF023803. Ribonuclease_P_prd. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular analysis of Saccharomyces cerevisiae chromosome I. On the number of genes and the identification of essential genes using temperature-sensitive-lethal mutations."
    Harris S.D., Cheng J., Pugh T.A., Pringle J.R.
    J. Mol. Biol. 225:53-65(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Purification and characterization of the nuclear RNase P holoenzyme complex reveals extensive subunit overlap with RNase MRP."
    Chamberlain J.R., Lee Y., Lane W.S., Engelke D.R.
    Genes Dev. 12:1678-1690(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN THE RNASE P COMPLEX BY MASS SPECTROMETRY.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Characterization and purification of Saccharomyces cerevisiae RNase MRP reveals a new unique protein component."
    Salinas K., Wierzbicki S., Zhou L., Schmitt M.E.
    J. Biol. Chem. 280:11352-11360(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE RNASE MRP COMPLEX BY MASS SPECTROMETRY.

Entry informationi

Entry nameiPOP5_YEAST
AccessioniPrimary (citable) accession number: P28005
Secondary accession number(s): D6VPI5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: June 24, 2015
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2230 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome I
    Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.