P28004 (PRP45_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pre-mRNA-processing protein 45

Gene names

Name:	PRP45
Synonyms:	FUN20
Ordered Locus Names:	YAL032C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	379 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in pre-mRNA splicing. Associated with the spliceosome throughout the splicing reactions, until after the second catalytic step. Ref.5 Ref.9
Subunit structure	Belongs to the CWC complex, a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least BUD13, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2. Interacts with CLF1, PRP22 and PRP46. Ref.9
Subcellular location	Nucleus Ref.5 Ref.7.
Miscellaneous	Present with 1670 molecules/cell in log phase SD medium. Ref.8
Sequence similarities	Belongs to the SNW family.
Sequence caution	The sequence CAA44455.1 differs from that shown. Reason: Frameshift at position 312.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus Spliceosome
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	generation of catalytic spliceosome for second transesterification step Inferred from mutant phenotype. Source: SGD
Cellular component	U2-type catalytic step 1 spliceosome Inferred from direct assay Ref.9. Source: SGD U2-type catalytic step 2 spliceosome Inferred from direct assay Ref.9. Source: SGD post-mRNA release spliceosomal complex Inferred from direct assay Ref.9. Source: SGD
Molecular function	protein binding Inferred from physical interaction Ref.6. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PRP46	Q12417	6	EBI-20640,EBI-710
SYF1	Q04048	3	EBI-20640,EBI-540

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 379	379	Pre-mRNA-processing protein 45		PRO_0000084825

Sequences

	Sequence		Length	Mass (Da)
	P28004 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: FE5A724186D7FE84	FASTA	379	42,483
	10 20 30 40 50 60 MFSNRLPPPK HSQGRVSTAL SSDRVEPAIL TDQIAKNVKL DDFIPKRQSN FELSVPLPTK 70 80 90 100 110 120 AEIQECTART KSYIQRLVNA KLANSNNRAS SRYVTETHQA PANLLLNNSH HIEVVSKQMD 130 140 150 160 170 180 PLLPRFVGKK ARKVVAPTEN DEVVPVLHMD GSNDRGEADP NEWKIPAAVS NWKNPNGYTV 190 200 210 220 230 240 ALERRVGKAL DNENNTINDG FMKLSEALEN ADKKARQEIR SKMELKRLAM EQEMLAKESK 250 260 270 280 290 300 LKELSQRARY HNGTPQTGAI VKPKKQTSTV ARLKELAYSQ GRDVSEKIIL GAAKRSEQPD 310 320 330 340 350 360 LQYDSRFFTR GANASAKRHE DQVYDNPLFV QQDIESIYKT NYEKLDEAVN VKSEGASGSH 370 GPIQFTKAES DDKSDNYGA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular analysis of Saccharomyces cerevisiae chromosome I. On the number of genes and the identification of essential genes using temperature-sensitive-lethal mutations." Harris S.D., Cheng J., Pugh T.A., Pringle J.R. J. Mol. Biol. 225:53-65(1992) [PubMed: 1583694] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae." Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K. Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed: 7731988] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Vo D.T. Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Functional mapping of Saccharomyces cerevisiae Prp45 identifies the SNW domain as essential for viability." Martinkova K., Lebduska P., Skruzny M., Folk P., Puta F. J. Biochem. 132:557-563(2002) [PubMed: 12359070] [Abstract] Cited for: FUNCTION, DOMAIN, SUBCELLULAR LOCATION.
[6]	"Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs." Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L. Mol. Cell. Biol. 22:2011-2024(2002) [PubMed: 11884590] [Abstract] Cited for: MASS SPECTROMETRY, IDENTIFICATION IN THE CWC COMPLEX.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Identification and characterization of Prp45p and Prp46p, essential pre-mRNA splicing factors." Albers M., Diment A., Muraru M., Russell C.S., Beggs J.D. RNA 9:138-150(2003) [PubMed: 12554883] [Abstract] Cited for: FUNCTION, INTERACTION WITH CLF1; PRP22 AND PRP46, ASSOCIATION WITH THE SPLICEOSOME.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X62577 Genomic DNA. Translation: CAA44455.1. Frameshift. U12980 Genomic DNA. Translation: AAC05000.1. BK006935 Genomic DNA. Translation: DAA06956.1.
PIR	S23409.
RefSeq	NP_009370.1. NM_001178177.1.
3D structure databases
ProteinModelPortal	P28004.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2774N.
IntAct	P28004. 34 interactions.
MINT	MINT-409776.
STRING	P28004.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YAL032C; YAL032C; YAL032C.
GeneID	851201.
KEGG	sce:YAL032C.
NMPDR	fig|4932.3.peg.49.
Organism-specific databases
CYGD	YAL032c.
SGD	S000000030. PRP45.
Phylogenomic databases
eggNOG	fuNOG06899.
GeneTree	EFGT00050000005023.
HOGENOM	HBG203624.
OMA	PIQFTKA.
OrthoDB	EOG4FJCK5.
Gene expression databases
ArrayExpress	P28004.
Genevestigator	P28004.
GermOnline	YAL032C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR017862. SKI-int_prot_SKIP. IPR004015. SKI-int_prot_SKIP_SNW-dom. [Graphical view]
KO	K06063.
PANTHER	PTHR12096. SKIP_SNW. 1 hit.
Pfam	PF02731. SKIP_SNW. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	968058.

Entry information

Entry name

PRP45_YEAST

Accession

Primary (citable) accession number: P28004
Secondary accession number(s): D6VPI6

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	October 1, 1996
Last modified:	January 25, 2012
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program